################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AviII BamHI BbrPI BbsI BciVI BclI BfrI BglII BpuAI BsaBI BseAI BsgI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MfeI MflI MluI MroI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI SalI ScaI SfiI SfuI SnaBI SpeI SphI SspBI SspI SwaI TfiI XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 293 GGTACC G^GTACC AgeI 1297 ACCGGT A^CCGGT AlwI 319 GGATC GGATC(4/5) Asp700I 688 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 293 GGTACC G^GTACC AspI 1033 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1110 CCTAGG C^CTAGG BbvCI 738 CCTCAGC CCTCAGC(-5/-2) BcgI 1370 CGAAAATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1110 CCTAGG C^CTAGG BlpI 661 GCTGAGC GC^T[ACGT]AGC Bpu1102I 661 GCTGAGC GC^T[ACGT]AGC BsaAI 244 TACGTG [CT]AC^GT[GA] BsaI 1307 GGTCTC GGTCTC(1/5) BsiWI 242 CGTACG C^GTACG BssHII 499 GCGCGC G^CGCGC BssSI 54 CTCGTG CACGAG(-5/-1) CelII 661 GCTGAGC GC^T[ACGT]AGC CpoI 1299 CGGTCCG CG^G[AT]CCG DrdI 1185 GACGGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC KpnI 293 GGTACC GGTAC^C MaeIII 1146 GTCAC ^GT[ACGT]AC NotI 214 GCGGCCGC GC^GGCCGC PflFI 1033 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 995 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1297 ACCGGT A^CCGGT RsrII 1299 CGGTCCG CG^G[AT]CCG SacI 103 GAGCTC GAGCT^C SapI 113 GCTCTTC GCTCTTC(1/4) SbfI 712 CCTGCAGG CCTGCA^GG SexAI 484 ACCTGGT A^CC[AT]GGT SfaNI 900 GCATC GCATC(5/9) Sse8387I 712 CCTGCAGG CCTGCA^GG StuI 386 AGGCCT AGG^CCT Tsp45I 1146 GTCAC ^GT[GC]AC Tsp509I 1424 AATT ^AATT TspRI 1165 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1033 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 995 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 688 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### BalI 446 TGGCCA TGG^CCA 1266 TGGCCA BfaI 1111 CTAG C^TAG 1396 CTAG BglI 37 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 157 GCCTTCACGGC BpmI 193 CTCCAG CTGGAG(16/14) 586 CTGGAG BsaWI 347 ACCGGA [AT]^CCGG[AT] 1297 ACCGGT BsiEI 108 CGGCCG CG[GA][CT]^CG 215 CGGCCG BsmBI 724 GAGACG CGTCTC(1/5) 760 GAGACG BsrBI 111 CCGCTC CCGCTC(-3/-3) 1346 GAGCGG BsrI 195 CCAGT ACTGG(1/-1) 1386 CCAGT EagI 108 CGGCCG C^GGCCG 215 CGGCCG EarI 114 CTCTTC CTCTTC(1/4) 565 GAAGAG EclXI 108 CGGCCG C^GGCCG 215 CGGCCG Eco52I 108 CGGCCG C^GGCCG 215 CGGCCG EcoNI 468 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1171 CCTTTTCCAGG HinfI 745 GACTC G^A[ACGT]TC 1411 GACTC HphI 455 TCACC GGTGA(8/7) 470 TCACC Ksp632I 114 CTCTTC CTCTTC(1/4) 565 GAAGAG MaeI 1111 CTAG C^TAG 1396 CTAG MaeII 245 ACGT A^CGT 532 ACGT MluNI 446 TGGCCA TGG^CCA 1266 TGGCCA MscI 446 TGGCCA TGG^CCA 1266 TGGCCA MseI 755 TTAA T^TAA 1324 TTAA PleI 745 GACTC GAGTC(4/5) 1411 GACTC PstI 713 CTGCAG CTGCA^G 790 CTGCAG SfcI 713 CTGCAG C^T[GA][CT]AG 790 CTGCAG SmlI 382 CTCAAG C^T[CT][GA]AG 511 CTCAAG TaqI 151 TCGA T^CGA 782 TCGA Tru9I 755 TTAA T^TAA 1324 TTAA TthHB8I 151 TCGA T^CGA 782 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI KspI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PpuMI RsaI SacII Sau3AI Sau96I ScrFI SfoI SgrAI SmaI StyI TseI XmaCI XmaI