(Linear) (Six-base) MAPSORT of: KIBB9733.seq Check: 1941 from: 1 to: 1449 KIBB9733.orf With 182 enzymes: SgfI * March 22, 2012 15:46 .. Acc65I G'GTAC_C Cuts at: 0 293 1449 Size: 293 1156 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 679 1449 Size: 679 770 AgeI A'CCGG_T Cuts at: 0 1297 1449 Size: 1297 152 AleI CACnn'nnGTG Cuts at: 0 475 1449 Size: 475 974 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1231 1263 1449 Size: 1231 32 186 Fragments arranged by size: 1231 186 32 ApaI G_GGCC'C Cuts at: 0 92 646 799 818 941 1094 1280 1449 Size: 92 554 153 19 123 153 186 169 Fragments arranged by size: 554 186 169 153 153 123 92 19 AvaI C'yCGr_G Cuts at: 0 619 637 1087 1272 1279 1449 Size: 619 18 450 185 7 170 Fragments arranged by size: 619 450 185 170 18 7 AvrII C'CTAG_G Cuts at: 0 1110 1449 Size: 1110 339 BanI G'GyrC_C Cuts at: 0 165 202 293 309 506 888 918 1140 Size: 165 37 91 16 197 382 30 222 Cuts at: 1140 1449 Size: 309 Fragments arranged by size: 382 309 222 197 165 91 37 30 16 BanII G_rGCy'C Cuts at: 0 92 107 127 253 286 611 646 799 Size: 92 15 20 126 33 325 35 153 Cuts at: 799 818 941 1069 1094 1280 1449 Size: 19 123 128 25 186 169 Fragments arranged by size: 325 186 169 153 128 126 123 92 35 33 25 20 19 15 BbeI G_GCGC'C Cuts at: 0 169 206 313 510 892 1144 1449 Size: 169 37 107 197 382 252 305 Fragments arranged by size: 382 305 252 197 169 107 37 BbvCI CC'TCA_GC Cuts at: 0 739 1449 Size: 739 710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1359 1393 1449 Size: 1359 34 56 Fragments arranged by size: 1359 56 34 BglI GCCn_nnn'nGGC Cuts at: 0 43 163 1449 Size: 43 120 1286 Fragments arranged by size: 1286 120 43 BlpI GC'TnA_GC Cuts at: 0 662 1449 Size: 662 787 Bme1580I G_kGCm'C Cuts at: 0 61 92 646 799 818 941 1094 1280 Size: 61 31 554 153 19 123 153 186 Cuts at: 1280 1449 Size: 169 Fragments arranged by size: 554 186 169 153 153 123 61 31 19 BmgBI CAC'GTC Cuts at: 0 533 1449 Size: 533 916 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 783 1406 1449 Size: 783 623 43 Fragments arranged by size: 783 623 43 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 607 1449 Size: 178 429 842 Fragments arranged by size: 842 429 178 Bpu10I CC'TnA_GC Cuts at: 0 739 1449 Size: 739 710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 496 1449 Size: 367 129 953 Fragments arranged by size: 953 367 129 BsaI GGTCTCn'nnnn_ Cuts at: 0 1313 1449 Size: 1313 136 BsaAI yAC'GTr Cuts at: 0 246 1449 Size: 246 1203 BsaHI Gr'CG_yC Cuts at: 0 166 203 310 368 507 538 763 889 Size: 166 37 107 58 139 31 225 126 Cuts at: 889 1126 1141 1449 Size: 237 15 308 Fragments arranged by size: 308 237 225 166 139 126 107 58 37 31 15 BsaWI w'CCGG_w Cuts at: 0 347 1297 1449 Size: 347 950 152 Fragments arranged by size: 950 347 152 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1231 1261 1449 Size: 1231 30 188 Fragments arranged by size: 1231 188 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 228 583 586 671 1449 Size: 228 355 3 85 778 Fragments arranged by size: 778 355 228 85 3 BseYI C'CCAG_C Cuts at: 0 339 826 1449 Size: 339 487 623 Fragments arranged by size: 623 487 339 BsiEI CG_ry'CG Cuts at: 0 111 218 1449 Size: 111 107 1231 Fragments arranged by size: 1231 111 107 BsiHKAI G_wGCw'C Cuts at: 0 107 404 986 1449 Size: 107 297 582 463 Fragments arranged by size: 582 463 297 107 BsiWI C'GTAC_G Cuts at: 0 242 1449 Size: 242 1207 BsmBI CGTCTCn'nnnn_ Cuts at: 0 718 754 1449 Size: 718 36 695 Fragments arranged by size: 718 695 36 Bsp1286I G_dGCh'C Cuts at: 0 61 92 107 127 253 286 404 611 Size: 61 31 15 20 126 33 118 207 Cuts at: 611 646 799 818 941 986 1069 1094 1280 Size: 35 153 19 123 45 83 25 186 Cuts at: 1280 1449 Size: 169 Fragments arranged by size: 207 186 169 153 126 123 118 83 61 45 35 33 31 25 20 19 15 BsrBI CCG'CTC Cuts at: 0 113 1348 1449 Size: 113 1235 101 Fragments arranged by size: 1235 113 101 BsrFI r'CCGG_y Cuts at: 0 503 808 885 1131 1297 1449 Size: 503 305 77 246 166 152 Fragments arranged by size: 503 305 246 166 152 77 BssHII G'CGCG_C Cuts at: 0 499 1449 Size: 499 950 BssSI C'ACGA_G Cuts at: 0 54 1449 Size: 54 1395 BtgI C'CryG_G Cuts at: 0 212 371 541 908 1449 Size: 212 159 170 367 541 Fragments arranged by size: 541 367 212 170 159 BtsI GCAGTG_nn' Cuts at: 0 1171 1449 Size: 1171 278 DrdI GACnn_nn'nnGTC Cuts at: 0 1191 1449 Size: 1191 258 EaeI y'GGCC_r Cuts at: 0 108 215 446 1266 1449 Size: 108 107 231 820 183 Fragments arranged by size: 820 231 183 108 107 EagI C'GGCC_G Cuts at: 0 108 215 1449 Size: 108 107 1234 Fragments arranged by size: 1234 108 107 EarI CTCTTCn'nnn_ Cuts at: 0 120 560 1449 Size: 120 440 889 Fragments arranged by size: 889 440 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 24 865 953 1449 Size: 24 841 88 496 Fragments arranged by size: 841 496 88 24 EcoICRI GAG'CTC Cuts at: 0 105 1449 Size: 105 1344 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 607 679 1449 Size: 178 429 72 770 Fragments arranged by size: 770 429 178 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 472 1175 1449 Size: 472 703 274 Fragments arranged by size: 703 472 274 EcoO109I rG'GnC_Cy Cuts at: 0 88 89 336 642 643 795 796 815 Size: 88 1 247 306 1 152 1 19 Cuts at: 815 855 928 938 1276 1288 1449 Size: 40 73 10 338 12 161 Fragments arranged by size: 338 306 247 161 152 88 73 40 19 12 10 1 1 1 HaeII r_GCGC'y Cuts at: 0 49 169 206 313 510 886 892 1144 Size: 49 120 37 107 197 376 6 252 Cuts at: 1144 1449 Size: 305 Fragments arranged by size: 376 305 252 197 120 107 49 37 6 KasI G'GCGC_C Cuts at: 0 165 202 309 506 888 1140 1449 Size: 165 37 107 197 382 252 309 Fragments arranged by size: 382 309 252 197 165 107 37 KpnI G_GTAC'C Cuts at: 0 297 1449 Size: 297 1152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1010 1449 Size: 1010 439 MscI TGG'CCA Cuts at: 0 448 1268 1449 Size: 448 820 181 Fragments arranged by size: 820 448 181 MslI CAynn'nnrTG Cuts at: 0 460 475 1262 1316 1449 Size: 460 15 787 54 133 Fragments arranged by size: 787 460 133 54 15 MspA1I CmG'CkG Cuts at: 0 214 543 835 910 1449 Size: 214 329 292 75 539 Fragments arranged by size: 539 329 292 214 75 NaeI GCC'GGC Cuts at: 0 505 887 1133 1449 Size: 505 382 246 316 Fragments arranged by size: 505 382 316 246 NarI GG'CG_CC Cuts at: 0 166 203 310 507 889 1141 1449 Size: 166 37 107 197 382 252 308 Fragments arranged by size: 382 308 252 197 166 107 37 NgoMIV G'CCGG_C Cuts at: 0 503 885 1131 1449 Size: 503 382 246 318 Fragments arranged by size: 503 382 318 246 NotI GC'GGCC_GC Cuts at: 0 215 1449 Size: 215 1234 PasI CC'CwG_GG Cuts at: 0 315 1449 Size: 315 1134 PflMI CCAn_nnn'nTGG Cuts at: 0 1001 1449 Size: 1001 448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1051 1083 1231 1263 1449 Size: 1051 32 148 32 186 Fragments arranged by size: 1051 186 148 32 32 PpuMI rG'GwC_Cy Cuts at: 0 336 855 928 1449 Size: 336 519 73 521 Fragments arranged by size: 521 519 336 73 PspOMI G'GGCC_C Cuts at: 0 88 642 795 814 937 1090 1276 1449 Size: 88 554 153 19 123 153 186 173 Fragments arranged by size: 554 186 173 153 153 123 88 19 PstI C_TGCA'G Cuts at: 0 717 794 1449 Size: 717 77 655 Fragments arranged by size: 717 655 77 RsrII CG'GwC_CG Cuts at: 0 1300 1449 Size: 1300 149 SacI G_AGCT'C Cuts at: 0 107 1449 Size: 107 1342 SacII CC_GC'GG Cuts at: 0 215 544 911 1449 Size: 215 329 367 538 Fragments arranged by size: 538 367 329 215 SanDI GG'GwC_CC Cuts at: 0 336 928 1449 Size: 336 592 521 Fragments arranged by size: 592 521 336 SapI GCTCTTCn'nnn_ Cuts at: 0 120 1449 Size: 120 1329 SbfI CC_TGCA'GG Cuts at: 0 717 1449 Size: 717 732 SexAI A'CCwGG_T Cuts at: 0 484 1449 Size: 484 965 SfcI C'TryA_G Cuts at: 0 713 790 1449 Size: 713 77 659 Fragments arranged by size: 713 659 77 SfoI GGC'GCC Cuts at: 0 167 204 311 508 890 1142 1449 Size: 167 37 107 197 382 252 307 Fragments arranged by size: 382 307 252 197 167 107 37 SgrAI Cr'CCGG_yG Cuts at: 0 503 808 885 1449 Size: 503 305 77 564 Fragments arranged by size: 564 503 305 77 SmaI CCC'GGG Cuts at: 0 1089 1274 1281 1449 Size: 1089 185 7 168 Fragments arranged by size: 1089 185 168 7 SmlI C'TyrA_G Cuts at: 0 382 511 1449 Size: 382 129 938 Fragments arranged by size: 938 382 129 SrfI GCCC'GGGC Cuts at: 0 1281 1449 Size: 1281 168 StuI AGG'CCT Cuts at: 0 388 1449 Size: 388 1061 StyI C'CwwG_G Cuts at: 0 287 425 1110 1449 Size: 287 138 685 339 Fragments arranged by size: 685 339 287 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 487 720 1449 Size: 487 233 729 Fragments arranged by size: 729 487 233 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1284 1316 1449 Size: 1284 32 133 Fragments arranged by size: 1284 133 32 Tth111I GACn'n_nGTC Cuts at: 0 1036 1449 Size: 1036 413 XmaI C'CCGG_G Cuts at: 0 1087 1272 1279 1449 Size: 1087 185 7 170 Fragments arranged by size: 1087 185 170 7 XmnI GAAnn'nnTTC Cuts at: 0 692 1449 Size: 692 757 Enzymes that do cut: Acc65I AcuI AgeI AleI AloI ApaI AvaI AvrII BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PpiI PpuMI PspOMI PstI RsrII SacI SacII SanDI SapI SbfI SexAI SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII TstI Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AhdI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbsI BciVI BclI BfrBI BglII BmrI BmtI BsaBI BsgI BsmI BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI NcoI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII SalI ScaI SfiI SgfI SnaBI SpeI SphI SspI SwaI TatI XbaI XcmI XhoI ZraI