Restriction map for
pFN21AE5024
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# NO CUT ENZYMES ( 78 enzymes) #
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AccIII AclI AgeI Alw44I ApaLI
AscI AseI AsnI AviII BalI
BbeI BbrPI BciVI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoRI EcoRV FspI
KasI MamI MluI MluNI MroI
MscI NarI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1770 GACGTC GACGT^C
Acc65I 211 GGTACC G^GTACC
AccI 2472 GTCTAC GT^[AC][GT]AC
AflII 3238 CTTAAG C^TTAAG
AhdI 2625 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3426 AGCGCT AGC^GCT
ApaI 1497 GGGCCC GGGCC^C
Asp718I 211 GGTACC G^GTACC
AspEI 2625 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3284 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1521 CCTAGG C^CTAGG
BanI 211 GGTACC G^G[CT][GA]CC
BclI 2324 TGATCA T^GATCA
BfrI 3238 CTTAAG C^TTAAG
BlnI 1521 CCTAGG C^CTAGG
BsaWI 1123 ACCGGA [AT]^CCGG[AT]
BsmBI 1768 GAGACG CGTCTC(1/5)
BspHI 3162 TCATGA T^CATGA
BspMI 1061 GCAGGT ACCTGC(4/8)
BsrFI 3592 GCCGGC [GA]^CCGG[CT]
BstEII 1596 GGTCACC G^GT[ACGT]ACC
BstPI 1596 GGTCACC G^GT[ACGT]ACC
Cfr10I 3592 GCCGGC [GA]^CCGG[CT]
Eam1105I 2625 GACATCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3426 AGCGCT AGC^GCT
EcoNI 517 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1596 GGTCACC G^GT[ACGT]ACC
EcoT22I 2068 ATGCAT ATGCA^T
FbaI 2324 TGATCA T^GATCA
FseI 3591 GGCCGGCC GGCCGG^CC
HindIII 1777 AAGCTT A^AGCTT
HpaI 13 GTTAAC GTT^AAC
KpnI 211 GGTACC GGTAC^C
KspI 1661 CCGCGG CCGC^GG
MfeI 442 CAATTG C^AATTG
MunI 442 CAATTG C^AATTG
NaeI 3592 GCCGGC GCC^GGC
NgoMIV 3592 GCCGGC G^CCGGC
NsiI 2068 ATGCAT ATGCA^T
PaeR7I 2281 CTCGAG C^TCGAG
PflFI 3284 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 248 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2967 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3162 TCATGA T^CATGA
SacI 1635 GAGCTC GAGCT^C
SacII 1661 CCGCGG CCGC^GG
SspI 468 AATATT AAT^ATT
Tth111I 3284 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2281 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 1071 CGCG CG^CG
1662 CGCG
AcsI 2157 AAATTT [GA]^AATT[CT]
2698 AAATTT
AcyI 1770 GACGTC G[GA]^CG[CT]C
2242 GACGCC
AocI 2206 CCTGAGG CC^T[ACGT]AGG
2998 CCTCAGG
ApoI 2157 AAATTT [GA]^AATT[CT]
2698 AAATTT
AspHI 312 GAGCAC G[AT]GC[AT]^C
1635 GAGCTC
AvaI 244 CTCGGG C^[CT]CG[GA]G
2281 CTCGAG
BamHI 2669 GGATCC G^GATCC
3393 GGATCC
BbsI 2197 GAAGAC GAAGAC(2/6)
3048 GTCTTC
BbvCI 215 CCTCAGC CCTCAGC(-5/-2)
886 CCTCAGC
BcgI 3146 CGAGTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3662 GCAAGGAGATCG
BglI 1466 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1658 GCCCCGCGGGC
BpuAI 2197 GAAGAC GAAGAC(2/6)
3048 GTCTTC
BsaHI 1770 GACGTC G[GA]^CG[CT]C
2242 GACGCC
BsgI 3278 CTGCAC GTGCAG(16/14)
1059 GTGCAG
BsiHKAI 312 GAGCAC G[AT]GC[AT]^C
1635 GAGCTC
BsoBI 244 CTCGGG C^[CT]CG[GA]G
2281 CTCGAG
BssSI 1272 CACGAG CACGAG(-5/-1)
3675 CACGAG
BstUI 1071 CGCG CG^CG
1662 CGCG
Bsu36I 2206 CCTGAGG CC^T[ACGT]AGG
2998 CCTCAGG
DraI 954 TTTAAA TTT^AAA
2701 TTTAAA
Eco81I 2206 CCTGAGG CC^T[ACGT]AGG
2998 CCTCAGG
Hin1I 1770 GACGTC G[GA]^CG[CT]C
2242 GACGCC
MvnI 1071 CGCG CG^CG
1662 CGCG
NcoI 80 CCATGG C^CATGG
2123 CCATGG
NdeI 1289 CATATG CA^TATG
2071 CATATG
ScaI 1027 AGTACT AGT^ACT
1276 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI Asp700I AvaII BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI