Restriction map for
pFN21AE5024
(Circular) (Six-base) MAPSORT of: pFN21AE5024.seq Check: 3464 from: 1 to: 8386
pFN21AE5024 8386 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3779 6196 278
Size: 53 83 186 936 2243 2417 2468
Fragments arranged by size:
2468 2417 2243 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4478 5729 1937
Size: 2541 1251 4594
Fragments arranged by size:
4594 2541 1251
Acc65I G'GTAC_C
Cuts at: 2216 5707 2216
Size: 3491 4895
AclI AA'CG_TT
Cuts at: 1563 6513 6886 1563
Size: 4950 373 3063
Fragments arranged by size:
4950 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3357 3770 4388 6449 7833 8101 1632
Size: 1725 413 618 2061 1384 268 1917
Fragments arranged by size:
2061 1917 1725 1384 618 413 268
AfeI AGC'GCT
Cuts at: 5433 5433
Size: 8386
AflII C'TTAA_G
Cuts at: 820 1017 5243 820
Size: 197 4226 3963
Fragments arranged by size:
4226 3963 197
AflIII A'CryG_T
Cuts at: 1203 4854 5126 5265 7285 8262 1203
Size: 3651 272 139 2020 977 1327
Fragments arranged by size:
3651 2020 1327 977 272 139
AgeI A'CCGG_T
Cuts at: 7217 7217
Size: 8386
AhdI GACnn_n'nnGTC
Cuts at: 4635 7115 4635
Size: 2480 5906
AleI CACnn'nnGTG
Cuts at: 1166 2324 3972 4604 1166
Size: 1158 1648 632 4948
Fragments arranged by size:
4948 1648 1158 632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8386
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 8354
AlwNI CAG_nnn'CTG
Cuts at: 2590 2603 3072 3793 4498 5412 6129 7701 2590
Size: 13 469 721 705 914 717 1572 3275
Fragments arranged by size:
3275 1572 914 721 717 705 469 13
ApaI G_GGCC'C
Cuts at: 3506 3506
Size: 8386
ApaLI G'TGCA_C
Cuts at: 6443 7599 6443
Size: 1156 7230
ApoI r'AATT_y
Cuts at: 1493 4162 4703 5695 5921 6073 8182 1493
Size: 2669 541 992 226 152 2109 1697
Fragments arranged by size:
2669 2109 1697 992 541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6940 160
Size: 872 5908 1606
Fragments arranged by size:
5908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8386
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 4286 5711 1870
Size: 73 306 2037 1425 4545
Fragments arranged by size:
4545 2037 1425 306 73
AvrII C'CTAG_G
Cuts at: 3526 3526
Size: 8386
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2134 2167 2134
Size: 33 8353
BamHI G'GATC_C
Cuts at: 4674 5398 5716 6083 4674
Size: 724 318 367 6977
Fragments arranged by size:
6977 724 367 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 5707 7162 618
Size: 325 217 627 429 3491 1455 1842
Fragments arranged by size:
3491 1842 1455 627 429 325 217
BanII G_rGCy'C
Cuts at: 1391 3506 3537 3644 4902 5583 5705 1391
Size: 2115 31 107 1258 681 122 4072
Fragments arranged by size:
4072 2115 1258 681 122 107 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4209 5046 928
Size: 971 2310 837 4268
Fragments arranged by size:
4268 2310 971 837
BbvCI CC'TCA_GC
Cuts at: 2221 2892 2221
Size: 671 7715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 5656 5690 6577 6611 1903
Size: 34 3203 34 482 34 887 34 3678
Fragments arranged by size:
3678 3203 887 482 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6278 7488 6278
Size: 1210 7176
BclI T'GATC_A
Cuts at: 1548 4329 1548
Size: 2781 5605
BfrBI ATG'CAT
Cuts at: 4075 4075
Size: 8386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3477 3669 6997
Size: 107 122 71 631 605 1805 192 3328
Cuts at: 6997 136
Size: 1525
Fragments arranged by size:
3328 1805 1525 631 605 192 122 107
71
BglII A'GATC_T
Cuts at: 3924 4876 5466 8381 3924
Size: 952 590 2915 3929
Fragments arranged by size:
3929 2915 952 590
BlpI GC'TnA_GC
Cuts at: 5803 5803
Size: 8386
Bme1580I G_kGCm'C
Cuts at: 1790 3506 5158 5261 6447 7603 1790
Size: 1716 1652 103 1186 1156 2573
Fragments arranged by size:
2573 1716 1652 1186 1156 103
BmgBI CAC'GTC
Cuts at: 1318 5035 1318
Size: 3717 4669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2276 2332 3909
Size: 416 53 83 399 24 859 56 1577
Cuts at: 3909 4097 4598 4896 5066 6117 7075 442
Size: 188 501 298 170 1051 958 1753
Fragments arranged by size:
1753 1577 1051 958 859 501 416 399
298 188 170 83 56 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8386
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4498 4498
Size: 8386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 3297 5205 5552 5579 5624 7046 1239
Size: 1058 1000 1908 347 27 45 1422 2579
Fragments arranged by size:
2579 1908 1422 1058 1000 347 45 27
Bpu10I CC'TnA_GC
Cuts at: 2221 2892 3255 3638 3762 2221
Size: 671 363 383 124 6845
Fragments arranged by size:
6845 671 383 363 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2511 6511 7376 7674 2511
Size: 4000 865 298 3223
Fragments arranged by size:
4000 3223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2827 4686 4786 7049 882
Size: 617 1328 1859 100 2263 2219
Fragments arranged by size:
2263 2219 1859 1328 617 100
BsaAI yAC'GTr
Cuts at: 493 1204 6177 493
Size: 711 4973 2702
Fragments arranged by size:
4973 2702 711
BsaBI GATnn'nnATC
Cuts at: 8380 8380
Size: 8386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3776 4248 6193 6575
Size: 53 83 186 936 2243 472 1945 382
Cuts at: 6575 7210 275
Size: 635 1451
Fragments arranged by size:
2243 1945 1451 936 635 472 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3128 6819 7217 7491 7638 8049 3128
Size: 3691 398 274 147 411 3465
Fragments arranged by size:
3691 3465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3082 3112 3155 3185 4370
Size: 30 165 30 1679 30 43 30 1185
Cuts at: 4370 4400 5453 5483 1178
Size: 30 1053 30 4081
Fragments arranged by size:
4081 1679 1185 1053 165 43 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2197 4562 4992 5134 1186
Size: 1011 2365 430 142 4438
Fragments arranged by size:
4438 2365 1011 430 142
BseYI C'CCAG_C
Cuts at: 2392 2792 2815 2921 2982 3646 4462 4538 4887
Size: 400 23 106 61 664 816 76 349
Cuts at: 4887 7589 8085 2392
Size: 2702 496 2693
Fragments arranged by size:
2702 2693 816 664 496 400 349 106
76 61 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3085 5268 1163
Size: 573 1349 2183 4281
Fragments arranged by size:
4281 2183 1349 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5836 6597 7625 1540
Size: 275 186 3835 761 1028 2301
Fragments arranged by size:
3835 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2321 3644 6447 6532 7603 2321
Size: 1323 2803 85 1071 3104
Fragments arranged by size:
3104 2803 1323 1071 85
BsmI GAATG_Cn'
Cuts at: 2500 3047 4009 4077 5897 5990 2500
Size: 547 962 68 1820 93 4896
Fragments arranged by size:
4896 1820 962 547 93 68
BsmBI CGTCTCn'nnnn_
Cuts at: 3767 3767
Size: 8386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 3506 3537 3644 4902 5158 5261
Size: 399 531 1185 31 107 1258 256 103
Cuts at: 5261 5583 5705 6447 6532 7603 1391
Size: 322 122 742 85 1071 2174
Fragments arranged by size:
2174 1258 1185 1071 742 531 399 322
256 122 107 103 85 31
BspEI T'CCGG_A
Cuts at: 8049 8049
Size: 8386
BspHI T'CATG_A
Cuts at: 5167 6275 8005 5167
Size: 1108 1730 5548
Fragments arranged by size:
5548 1730 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 5741 844
Size: 2213 2684 3489
Fragments arranged by size:
3489 2684 2213
BsrBI CCG'CTC
Cuts at: 1394 1823 3706 3997 4565 6273 1394
Size: 429 1883 291 568 1708 3507
Fragments arranged by size:
3507 1883 1708 568 429 291
BsrDI GCAATG_nn'
Cuts at: 65 1248 6881 7055 65
Size: 1183 5633 174 1396
Fragments arranged by size:
5633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5597 7030 7217 1700
Size: 110 3787 1433 187 2869
Fragments arranged by size:
3787 2869 1433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8386
BssSI C'ACGA_G
Cuts at: 3277 5680 6446 7458 3277
Size: 2403 766 1012 4205
Fragments arranged by size:
4205 2403 1012 766
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3072 3156 3843 4812 794
Size: 2278 84 687 969 4368
Fragments arranged by size:
4368 2278 969 687 84
BstBI TT'CG_AA
Cuts at: 7190 7190
Size: 8386
BstEII G'GTnAC_C
Cuts at: 1184 3601 1184
Size: 2417 5969
BstXI CCAn_nnnn'nTGG
Cuts at: 2092 4454 5446 2092
Size: 2362 992 5032
Fragments arranged by size:
5032 2362 992
BstYI r'GATC_y
Cuts at: 1972 2433 3108 3924 4089 4674 4876 5398 5466
Size: 461 675 816 165 585 202 522 68
Cuts at: 5466 5716 6083 6468 6485 7937 8023 8035 8381
Size: 250 367 385 17 1452 86 12 346
Cuts at: 8381 1972
Size: 1977
Fragments arranged by size:
1977 1452 816 675 585 522 461 385
367 346 250 202 165 86 68 17
12
Bsu36I CC'TnA_GG
Cuts at: 4212 5004 4212
Size: 792 7594
BtgI C'CryG_G
Cuts at: 513 1065 2085 3666 4128 513
Size: 552 1020 1581 462 4771
Fragments arranged by size:
4771 1581 1020 552 462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4285 5465 487
Size: 683 56 3059 1180 3408
Fragments arranged by size:
3408 3059 1180 683 56
BtsI GCAGTG_nn'
Cuts at: 3012 3679 4713 5524 5626 5902 6696 6716 3012
Size: 667 1034 811 102 276 794 20 4682
Fragments arranged by size:
4682 1034 811 794 667 276 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5110 5145 529
Size: 35 4546 35 3770
Fragments arranged by size:
4546 3770 35 35
DraI TTT'AAA
Cuts at: 2961 4708 5690 2961
Size: 1747 982 5657
Fragments arranged by size:
5657 1747 982
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7393 809
Size: 507 6077 1802
Fragments arranged by size:
6077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 3931 5595
Size: 54 1425 50 275 18 555 1546 1664
Cuts at: 5595 5833 6722 8351 8
Size: 238 889 1629 43
Fragments arranged by size:
1664 1629 1546 1425 889 555 275 238
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5833 1537
Size: 275 4021 4090
Fragments arranged by size:
4090 4021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2728 3426 3582 4276 4489 4685 5212
Size: 720 655 698 156 694 213 196 527
Cuts at: 5212 6316 1353
Size: 1104 3423
Fragments arranged by size:
3423 1104 720 698 694 655 527 213
196 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6973 7347 7493 387
Size: 1064 138 5384 374 146 1280
Fragments arranged by size:
5384 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3642 3642
Size: 8386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 3297 3357 3770 4388 5205 5552
Size: 393 665 1000 60 413 618 817 347
Cuts at: 5552 5579 5624 6449 7046 7833 8101 1239
Size: 27 45 825 597 787 268 1524
Fragments arranged by size:
1524 1000 825 817 787 665 618 597
413 393 347 268 60 45 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2526 2526
Size: 8386
EcoO109I rG'GnC_Cy
Cuts at: 2098 2254 2367 2796 3502 3503 3909 2098
Size: 156 113 429 706 1 406 6575
Fragments arranged by size:
6575 706 429 406 156 113 1
EcoRI G'AATT_C
Cuts at: 5695 6073 5695
Size: 378 8008
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3974 5258 3974
Size: 1284 7102
FseI GG_CCGG'CC
Cuts at: 5601 5601
Size: 8386
FspI TGC'GCA
Cuts at: 6892 6892
Size: 8386
HaeII r_GCGC'y
Cuts at: 1678 3208 3873 5435 7533 1678
Size: 1530 665 1562 2098 2531
Fragments arranged by size:
2531 2098 1562 1530 665
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2948 2980 4074 4103 4106
Size: 32 135 32 1376 32 1094 29 3
Cuts at: 4106 4135 4169 4201 4250 4282 4370 4402 4406
Size: 29 34 32 49 32 88 32 4
Cuts at: 4406 4438 4616 4621 4648 4653 4861 4868 4893
Size: 32 178 5 27 5 208 7 25
Cuts at: 4893 4900 7027 7059 7101 7133 1373
Size: 7 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 1376 1094 208 178 135 88
49 42 34 32 32 32 32 32
32 32 32 32 29 29 27 25
7 7 5 5 4 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2020 4860 4981 5730 5976 669
Size: 988 281 82 2840 121 749 246 3079
Fragments arranged by size:
3079 2840 988 749 281 246 121 82
HindIII A'AGCT_T
Cuts at: 748 3782 748
Size: 3034 5352
HpaI GTT'AAC
Cuts at: 2020 5976 2020
Size: 3956 4430
KpnI G_GTAC'C
Cuts at: 2220 5711 2220
Size: 3491 4895
MfeI C'AATT_G
Cuts at: 2447 5985 2447
Size: 3538 4848
MluI A'CGCG_T
Cuts at: 8262 8262
Size: 8386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2736 4533 4932 5463 7500 7684 1305
Size: 1431 1797 399 531 2037 184 2007
Fragments arranged by size:
2037 2007 1797 1431 531 399 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6564
Fragments arranged by size:
6564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2324 3145 3972 4070 4604 5264
Size: 648 924 234 821 827 98 534 660
Cuts at: 5264 6344 6703 6862 518
Size: 1080 359 159 2042
Fragments arranged by size:
2042 1080 924 827 821 660 648 534
359 234 159 98
MspA1I CmG'CkG
Cuts at: 1606 2124 2136 2190 2443 2590 2603 2718 2750
Size: 518 12 54 253 147 13 115 32
Cuts at: 2750 2819 3333 3650 3668 3861 4934 5802 6479
Size: 69 514 317 18 193 1073 868 677
Cuts at: 6479 7627 7872 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1073 868 677 518 514 317
253 245 193 147 115 69 54 32
18 13 12
NaeI GCC'GGC
Cuts at: 5599 5599
Size: 8386
NcoI C'CATG_G
Cuts at: 513 1065 2085 4128 513
Size: 552 1020 2043 4771
Fragments arranged by size:
4771 2043 1020 552
NdeI CA'TA_TG
Cuts at: 387 3295 4077 387
Size: 2908 782 4696
Fragments arranged by size:
4696 2908 782
NgoMIV G'CCGG_C
Cuts at: 5597 5597
Size: 8386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8386
NotI GC'GGCC_GC
Cuts at: 5833 5833
Size: 8386
NsiI A_TGCA'T
Cuts at: 4077 4077
Size: 8386
NspI r_CATG'y
Cuts at: 1132 1229 3304 4858 4997 5130 5269 5744 7289
Size: 97 2075 1554 139 133 139 475 1545
Cuts at: 7289 1132
Size: 2229
Fragments arranged by size:
2229 2075 1554 1545 475 139 139 133
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8386
PciI A'CATG_T
Cuts at: 4854 5126 5265 7285 4854
Size: 272 139 2020 5955
Fragments arranged by size:
5955 2020 272 139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3572 4610 5412 5504 1108
Size: 2464 1038 802 92 3990
Fragments arranged by size:
3990 2464 1038 802 92
PfoI T'CCnGG_A
Cuts at: 3750 5503 3750
Size: 1753 6633
PmeI GTTT'AAAC
Cuts at: 5690 5690
Size: 8386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 6455 6487 7968 8000 3155
Size: 32 3268 32 1481 32 3541
Fragments arranged by size:
3541 3268 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2254
Size: 8386
PshAI GACnn'nnGTC
Cuts at: 1613 4976 1613
Size: 3363 5023
PsiI TTA'TAA
Cuts at: 5956 5956
Size: 8386
PspOMI G'GGCC_C
Cuts at: 3502 3502
Size: 8386
PstI C_TGCA'G
Cuts at: 830 2045 2424 2953 4059 4246 5163 5738 830
Size: 1215 379 529 1106 187 917 575 3478
Fragments arranged by size:
3478 1215 1106 917 575 529 379 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8386
PvuII CAG'CTG
Cuts at: 2443 2590 2603 3333 3861 2443
Size: 147 13 730 528 6968
Fragments arranged by size:
6968 730 528 147 13
SacI G_AGCT'C
Cuts at: 3644 3644
Size: 8386
SacII CC_GC'GG
Cuts at: 3669 3669
Size: 8386
SalI G'TCGA_C
Cuts at: 1936 5728 1936
Size: 3792 4594
SapI GCTCTTCn'nnn_
Cuts at: 2728 3426 5212 2728
Size: 698 1786 5902
Fragments arranged by size:
5902 1786 698
SbfI CC_TGCA'GG
Cuts at: 5738 5738
Size: 8386
ScaI AGT'ACT
Cuts at: 3034 3283 6634 8346 3034
Size: 249 3351 1712 3074
Fragments arranged by size:
3351 3074 1712 249
SfcI C'TryA_G
Cuts at: 826 1045 2041 2420 2949 3886 3970 4055 4242
Size: 219 996 379 529 937 84 85 187
Cuts at: 4242 4355 4771 5159 5734 6869 7550 7741 826
Size: 113 416 388 575 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 937 681 575 529 416
388 379 219 191 187 113 85 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8386
SmaI CCC'GGG
Cuts at: 1872 5713 1872
Size: 3841 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2526 4286 5243 6490 7391 7653
Size: 197 926 583 1760 957 1247 901 262
Cuts at: 7653 820
Size: 1553
Fragments arranged by size:
1760 1553 1247 957 926 901 583 262
197
SnaBI TAC'GTA
Cuts at: 493 6177 493
Size: 5684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8386
SphI G_CATG'C
Cuts at: 1132 5744 1132
Size: 4612 3774
SspI AAT'ATT
Cuts at: 5 52 2475 6310 5
Size: 47 2423 3835 2081
Fragments arranged by size:
3835 2423 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8386
StyI C'CwwG_G
Cuts at: 513 1065 2085 2322 2976 3177 3526 4128 4758
Size: 552 1020 237 654 201 349 602 630
Cuts at: 4758 4912 5825 513
Size: 154 913 3074
Fragments arranged by size:
3074 1020 913 654 630 602 552 349
237 201 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4418 4552 6413 6430 6583 6768 7213
Size: 1255 2838 134 1861 17 153 185 445
Cuts at: 7213 325
Size: 1498
Fragments arranged by size:
2838 1861 1498 1255 445 185 153 134
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3032 3281 6632
Size: 274 80 33 51 1443 1055 249 3351
Cuts at: 6632 8344 96
Size: 1712 138
Fragments arranged by size:
3351 1712 1443 1055 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 2689 2721 3082 3104 3114 3136 8346
Size: 32 315 32 361 22 10 22 5210
Cuts at: 8346 8378 2342
Size: 32 2350
Fragments arranged by size:
5210 2350 361 315 32 32 32 22
22 10
Tth111I GACn'n_nGTC
Cuts at: 1316 5292 1316
Size: 3976 4410
XbaI T'CTAG_A
Cuts at: 5722 5722
Size: 8386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 3330 5590 2329
Size: 1001 2260 5125
Fragments arranged by size:
5125 2260 1001
XhoI C'TCGA_G
Cuts at: 1943 4286 1943
Size: 2343 6043
XmaI C'CCGG_G
Cuts at: 1870 5711 1870
Size: 3841 4545
XmnI GAAnn'nnTTC
Cuts at: 2460 3167 4034 6515 2460
Size: 707 867 2481 4331
Fragments arranged by size:
4331 2481 867 707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3777 6194 276
Size: 53 83 186 936 2243 2417 2468
Fragments arranged by size:
2468 2417 2243 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BbeI BsiWI BstZ17I DraIII EcoRV FspAI
KasI NarI NruI PacI PmlI PspXI PsrI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI