(Linear) (Six-base) MAPSORT of: KIEE5024.seq Check: 9441 from: 1 to: 3681 KIEE5024.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AatII G_ACGT'C Cuts at: 0 1774 3681 Size: 1774 1907 AccI GT'mk_AC Cuts at: 0 2473 3681 Size: 2473 1208 Acc65I G'GTAC_C Cuts at: 0 211 3681 Size: 211 3470 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1352 1765 2383 3681 Size: 1352 413 618 1298 Fragments arranged by size: 1352 1298 618 413 AfeI AGC'GCT Cuts at: 0 3428 3681 Size: 3428 253 AflII C'TTAA_G Cuts at: 0 3238 3681 Size: 3238 443 AflIII A'CryG_T Cuts at: 0 2849 3121 3260 3681 Size: 2849 272 139 421 Fragments arranged by size: 2849 421 272 139 AhdI GACnn_n'nnGTC Cuts at: 0 2630 3681 Size: 2630 1051 AleI CACnn'nnGTG Cuts at: 0 319 1967 2599 3681 Size: 319 1648 632 1082 Fragments arranged by size: 1648 1082 632 319 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1150 1182 3681 Size: 1150 32 2499 Fragments arranged by size: 2499 1150 32 AlwNI CAG_nnn'CTG Cuts at: 0 585 598 1067 1788 2493 3407 3681 Size: 585 13 469 721 705 914 274 Fragments arranged by size: 914 721 705 585 469 274 13 ApaI G_GGCC'C Cuts at: 0 1501 3681 Size: 1501 2180 ApoI r'AATT_y Cuts at: 0 2157 2698 3681 Size: 2157 541 983 Fragments arranged by size: 2157 983 541 AvaI C'yCGr_G Cuts at: 0 244 2281 3681 Size: 244 2037 1400 Fragments arranged by size: 2037 1400 244 AvrII C'CTAG_G Cuts at: 0 1521 3681 Size: 1521 2160 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 129 162 3681 Size: 129 33 3519 Fragments arranged by size: 3519 129 33 BamHI G'GATC_C Cuts at: 0 2669 3393 3681 Size: 2669 724 288 Fragments arranged by size: 2669 724 288 BanI G'GyrC_C Cuts at: 0 211 3681 Size: 211 3470 BanII G_rGCy'C Cuts at: 0 1501 1532 1639 2897 3578 3681 Size: 1501 31 107 1258 681 103 Fragments arranged by size: 1501 1258 681 107 103 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 2204 3041 3681 Size: 2204 837 640 Fragments arranged by size: 2204 837 640 BbvCI CC'TCA_GC Cuts at: 0 216 887 3681 Size: 216 671 2794 Fragments arranged by size: 2794 671 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3135 3169 3651 3681 Size: 3135 34 482 30 Fragments arranged by size: 3135 482 34 30 BclI T'GATC_A Cuts at: 0 2324 3681 Size: 2324 1357 BfrBI ATG'CAT Cuts at: 0 2070 3681 Size: 2070 1611 BglI GCCn_nnn'nGGC Cuts at: 0 1472 1664 3681 Size: 1472 192 2017 Fragments arranged by size: 2017 1472 192 BglII A'GATC_T Cuts at: 0 1919 2871 3461 3681 Size: 1919 952 590 220 Fragments arranged by size: 1919 952 590 220 Bme1580I G_kGCm'C Cuts at: 0 1501 3153 3256 3681 Size: 1501 1652 103 425 Fragments arranged by size: 1652 1501 425 103 BmgBI CAC'GTC Cuts at: 0 3030 3681 Size: 3030 651 BmrI ACTGGGnnnn_n' Cuts at: 0 271 327 1904 2092 2593 2891 3061 3681 Size: 271 56 1577 188 501 298 170 620 Fragments arranged by size: 1577 620 501 298 271 188 170 56 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2493 3681 Size: 2493 1188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 1292 3200 3547 3574 3619 3681 Size: 292 1000 1908 347 27 45 62 Fragments arranged by size: 1908 1000 347 292 62 45 27 Bpu10I CC'TnA_GC Cuts at: 0 216 887 1250 1633 1757 3681 Size: 216 671 363 383 124 1924 Fragments arranged by size: 1924 671 383 363 216 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 506 3681 Size: 506 3175 BsaI GGTCTCn'nnnn_ Cuts at: 0 822 2681 2781 3681 Size: 822 1859 100 900 Fragments arranged by size: 1859 900 822 100 BsaHI Gr'CG_yC Cuts at: 0 1771 2243 3681 Size: 1771 472 1438 Fragments arranged by size: 1771 1438 472 BsaWI w'CCGG_w Cuts at: 0 1123 3681 Size: 1123 2558 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1077 1107 1150 1180 2365 2395 3448 3478 Size: 1077 30 43 30 1185 30 1053 30 Cuts at: 3478 3681 Size: 203 Fragments arranged by size: 1185 1077 1053 203 43 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 192 2557 2987 3129 3681 Size: 192 2365 430 142 552 Fragments arranged by size: 2365 552 430 192 142 BseYI C'CCAG_C Cuts at: 0 387 787 810 916 977 1641 2457 2533 Size: 387 400 23 106 61 664 816 76 Cuts at: 2533 2882 3681 Size: 349 799 Fragments arranged by size: 816 799 664 400 387 349 106 76 61 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1080 3263 3681 Size: 1080 2183 418 Fragments arranged by size: 2183 1080 418 BsiHKAI G_wGCw'C Cuts at: 0 316 1639 3681 Size: 316 1323 2042 Fragments arranged by size: 2042 1323 316 BsmI GAATG_Cn' Cuts at: 0 495 1042 2004 2072 3681 Size: 495 547 962 68 1609 Fragments arranged by size: 1609 962 547 495 68 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1762 3681 Size: 1762 1919 Bsp1286I G_dGCh'C Cuts at: 0 316 1501 1532 1639 2897 3153 3256 3578 Size: 316 1185 31 107 1258 256 103 322 Cuts at: 3578 3681 Size: 103 Fragments arranged by size: 1258 1185 322 316 256 107 103 103 31 BspHI T'CATG_A Cuts at: 0 3162 3681 Size: 3162 519 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1052 3681 Size: 1052 2629 BsrBI CCG'CTC Cuts at: 0 1701 1992 2560 3681 Size: 1701 291 568 1121 Fragments arranged by size: 1701 1121 568 291 BsrFI r'CCGG_y Cuts at: 0 3592 3681 Size: 3592 89 BssSI C'ACGA_G Cuts at: 0 1272 3675 3681 Size: 1272 2403 6 Fragments arranged by size: 2403 1272 6 BstAPI GCAn_nnn'nTGC Cuts at: 0 1067 1151 1838 2807 3681 Size: 1067 84 687 969 874 Fragments arranged by size: 1067 969 874 687 84 BstEII G'GTnAC_C Cuts at: 0 1596 3681 Size: 1596 2085 BstXI CCAn_nnnn'nTGG Cuts at: 0 87 2449 3441 3681 Size: 87 2362 992 240 Fragments arranged by size: 2362 992 240 87 BstYI r'GATC_y Cuts at: 0 428 1103 1919 2084 2669 2871 3393 3461 Size: 428 675 816 165 585 202 522 68 Cuts at: 3461 3681 Size: 220 Fragments arranged by size: 816 675 585 522 428 220 202 165 68 Bsu36I CC'TnA_GG Cuts at: 0 2207 2999 3681 Size: 2207 792 682 Fragments arranged by size: 2207 792 682 BtgI C'CryG_G Cuts at: 0 80 1661 2123 3681 Size: 80 1581 462 1558 Fragments arranged by size: 1581 1558 462 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2280 3460 3681 Size: 2280 1180 221 Fragments arranged by size: 2280 1180 221 BtsI GCAGTG_nn' Cuts at: 0 1007 1674 2708 3519 3621 3681 Size: 1007 667 1034 811 102 60 Fragments arranged by size: 1034 1007 811 667 102 60 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3105 3140 3681 Size: 3105 35 541 Fragments arranged by size: 3105 541 35 DraI TTT'AAA Cuts at: 0 956 2703 3681 Size: 956 1747 978 Fragments arranged by size: 1747 978 956 EaeI y'GGCC_r Cuts at: 0 380 1926 3590 3681 Size: 380 1546 1664 91 Fragments arranged by size: 1664 1546 380 91 EarI CTCTTCn'nnn_ Cuts at: 0 68 723 1421 1577 2271 2484 2680 3207 Size: 68 655 698 156 694 213 196 527 Cuts at: 3207 3681 Size: 474 Fragments arranged by size: 698 694 655 527 474 213 196 156 68 EcoICRI GAG'CTC Cuts at: 0 1637 3681 Size: 1637 2044 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 1292 1352 1765 2383 3200 3547 3574 Size: 292 1000 60 413 618 817 347 27 Cuts at: 3574 3619 3681 Size: 45 62 Fragments arranged by size: 1000 817 618 413 347 292 62 60 45 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 521 3681 Size: 521 3160 EcoO109I rG'GnC_Cy Cuts at: 0 93 249 362 791 1497 1498 1904 3681 Size: 93 156 113 429 706 1 406 1777 Fragments arranged by size: 1777 706 429 406 156 113 93 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1969 3253 3681 Size: 1969 1284 428 Fragments arranged by size: 1969 1284 428 FseI GG_CCGG'CC Cuts at: 0 3596 3681 Size: 3596 85 HaeII r_GCGC'y Cuts at: 0 1203 1868 3430 3681 Size: 1203 665 1562 251 Fragments arranged by size: 1562 1203 665 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 943 975 2069 2098 2101 2130 2164 2196 Size: 943 32 1094 29 3 29 34 32 Cuts at: 2196 2245 2277 2365 2397 2401 2433 2611 2616 Size: 49 32 88 32 4 32 178 5 Cuts at: 2616 2643 2648 2856 2863 2888 2895 3681 Size: 27 5 208 7 25 7 786 Fragments arranged by size: 1094 943 786 208 178 88 49 34 32 32 32 32 32 29 29 27 25 7 7 5 5 4 3 HincII GTy'rAC Cuts at: 0 15 2855 2976 3681 Size: 15 2840 121 705 Fragments arranged by size: 2840 705 121 15 HindIII A'AGCT_T Cuts at: 0 1777 3681 Size: 1777 1904 HpaI GTT'AAC Cuts at: 0 15 3681 Size: 15 3666 KpnI G_GTAC'C Cuts at: 0 215 3681 Size: 215 3466 MfeI C'AATT_G Cuts at: 0 442 3681 Size: 442 3239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 731 2528 2927 3458 3681 Size: 731 1797 399 531 223 Fragments arranged by size: 1797 731 531 399 223 MslI CAynn'nnrTG Cuts at: 0 85 319 1140 1967 2065 2599 3259 3681 Size: 85 234 821 827 98 534 660 422 Fragments arranged by size: 827 821 660 534 422 234 98 85 MspA1I CmG'CkG Cuts at: 0 119 131 185 438 585 598 713 745 Size: 119 12 54 253 147 13 115 32 Cuts at: 745 814 1328 1645 1663 1856 2929 3681 Size: 69 514 317 18 193 1073 752 Fragments arranged by size: 1073 752 514 317 253 193 147 119 115 69 54 32 18 13 12 NaeI GCC'GGC Cuts at: 0 3594 3681 Size: 3594 87 NcoI C'CATG_G Cuts at: 0 80 2123 3681 Size: 80 2043 1558 Fragments arranged by size: 2043 1558 80 NdeI CA'TA_TG Cuts at: 0 1290 2072 3681 Size: 1290 782 1609 Fragments arranged by size: 1609 1290 782 NgoMIV G'CCGG_C Cuts at: 0 3592 3681 Size: 3592 89 NsiI A_TGCA'T Cuts at: 0 2072 3681 Size: 2072 1609 NspI r_CATG'y Cuts at: 0 1299 2853 2992 3125 3264 3681 Size: 1299 1554 139 133 139 417 Fragments arranged by size: 1554 1299 417 139 139 133 PciI A'CATG_T Cuts at: 0 2849 3121 3260 3681 Size: 2849 272 139 421 Fragments arranged by size: 2849 421 272 139 PflMI CCAn_nnn'nTGG Cuts at: 0 1567 2605 3407 3499 3681 Size: 1567 1038 802 92 182 Fragments arranged by size: 1567 1038 802 182 92 PfoI T'CCnGG_A Cuts at: 0 1745 3498 3681 Size: 1745 1753 183 Fragments arranged by size: 1753 1745 183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1150 1182 3681 Size: 1150 32 2499 Fragments arranged by size: 2499 1150 32 PpuMI rG'GwC_Cy Cuts at: 0 249 3681 Size: 249 3432 PshAI GACnn'nnGTC Cuts at: 0 2971 3681 Size: 2971 710 PspOMI G'GGCC_C Cuts at: 0 1497 3681 Size: 1497 2184 PstI C_TGCA'G Cuts at: 0 40 419 948 2054 2241 3158 3681 Size: 40 379 529 1106 187 917 523 Fragments arranged by size: 1106 917 529 523 379 187 40 PvuII CAG'CTG Cuts at: 0 438 585 598 1328 1856 3681 Size: 438 147 13 730 528 1825 Fragments arranged by size: 1825 730 528 438 147 13 SacI G_AGCT'C Cuts at: 0 1639 3681 Size: 1639 2042 SacII CC_GC'GG Cuts at: 0 1664 3681 Size: 1664 2017 SapI GCTCTTCn'nnn_ Cuts at: 0 723 1421 3207 3681 Size: 723 698 1786 474 Fragments arranged by size: 1786 723 698 474 ScaI AGT'ACT Cuts at: 0 1029 1278 3681 Size: 1029 249 2403 Fragments arranged by size: 2403 1029 249 SfcI C'TryA_G Cuts at: 0 36 415 944 1881 1965 2050 2237 2350 Size: 36 379 529 937 84 85 187 113 Cuts at: 2350 2766 3154 3681 Size: 416 388 527 Fragments arranged by size: 937 529 527 416 388 379 187 113 85 84 36 SmlI C'TyrA_G Cuts at: 0 521 2281 3238 3681 Size: 521 1760 957 443 Fragments arranged by size: 1760 957 521 443 SspI AAT'ATT Cuts at: 0 470 3681 Size: 470 3211 StyI C'CwwG_G Cuts at: 0 80 317 971 1172 1521 2123 2753 2907 Size: 80 237 654 201 349 602 630 154 Cuts at: 2907 3681 Size: 774 Fragments arranged by size: 774 654 630 602 349 237 201 154 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2413 2547 3681 Size: 2413 134 1134 Fragments arranged by size: 2413 1134 134 TatI w'GTAC_w Cuts at: 0 1027 1276 3681 Size: 1027 249 2405 Fragments arranged by size: 2405 1027 249 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 337 369 684 716 1077 1099 1109 1131 Size: 337 32 315 32 361 22 10 22 Cuts at: 1131 3681 Size: 2550 Fragments arranged by size: 2550 361 337 315 32 32 22 22 10 Tth111I GACn'n_nGTC Cuts at: 0 3287 3681 Size: 3287 394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 324 1325 3585 3681 Size: 324 1001 2260 96 Fragments arranged by size: 2260 1001 324 96 XhoI C'TCGA_G Cuts at: 0 2281 3681 Size: 2281 1400 XmnI GAAnn'nnTTC Cuts at: 0 455 1162 2029 3681 Size: 455 707 867 1652 Fragments arranged by size: 1652 867 707 455 ZraI GAC'GTC Cuts at: 0 1772 3681 Size: 1772 1909 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AloI AlwNI ApaI ApoI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI ScaI SfcI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI AclI AgeI AlfI ApaLI AscI AseI AsiSI BbeI BciVI BlpI BmtI BsaAI BsaBI BsiEI BsiWI BspEI BsrDI BsrGI BssHII BstBI BstZ17I ClaI DraIII DrdI EagI EciI EcoRI EcoRV FspI FspAI KasI MluI MscI NarI NheI NotI NruI PacI PasI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XmaI