Restriction map for
pFN21AE2739
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccI AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BbrPI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BspDI
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
MaeII MamI MfeI MluI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RsrII SacI SalI SbfI ScaI
SfcI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp509I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 953 GGTACC G^GTACC
AccIII 481 TCCGGA T^CCGGA
AflIII 473 ACATGT A^C[GA][CT]GT
AocI 1042 CCTGAGG CC^T[ACGT]AGG
Asp718I 953 GGTACC G^GTACC
AviII 386 TGCGCA TGC^GCA
BalI 234 TGGCCA TGG^CCA
BamHI 732 GGATCC G^GATCC
BclI 998 TGATCA T^GATCA
BglI 43 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 461 AGATCT A^GATCT
BsaWI 481 TCCGGA [AT]^CCGG[AT]
BseAI 481 TCCGGA T^CCGGA
BsmBI 855 CGTCTC CGTCTC(1/5)
BspEI 481 TCCGGA T^CCGGA
BspHI 1976 TCATGA T^CATGA
BspLU11I 473 ACATGT A^CATGT
BsrDI 84 CATTGC GCAATG(2/0)
BssSI 661 CACGAG CACGAG(-5/-1)
BstXI 1910 CCAGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1042 CCTGAGG CC^T[ACGT]AGG
DrdI 73 GACATCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 402 CGGCCG C^GGCCG
EclXI 402 CGGCCG C^GGCCG
Eco52I 402 CGGCCG C^GGCCG
Eco81I 1042 CCTGAGG CC^T[ACGT]AGG
EcoNI 1881 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 998 TGATCA T^GATCA
FspI 386 TGCGCA TGC^GCA
HincII 1957 GTCAAC GT[CT]^[GA]AC
HindII 1957 GTCAAC GT[CT]^[GA]AC
KpnI 953 GGTACC GGTAC^C
KspI 1099 CCGCGG CCGC^GG
MluNI 234 TGGCCA TGG^CCA
MroI 481 TCCGGA T^CCGGA
MscI 234 TGGCCA TGG^CCA
MseI 1439 TTAA T^TAA
NaeI 1249 GCCGGC GCC^GGC
NgoMIV 1249 GCCGGC G^CCGGC
NotI 401 GCGGCCGC GC^GGCCGC
NspI 473 ACATGT [GA]CATG^[CT]
PshAI 612 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1527 CAGCTG CAG^CTG
RcaI 1976 TCATGA T^CATGA
SacII 1099 CCGCGG CCGC^GG
SexAI 881 ACCAGGT A^CC[AT]GGT
SfiI 42 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1248 CGCCGGCG C[GA]^CCGG[CT]G
Tru9I 1439 TTAA T^TAA
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# TWO CUT ENZYMES ( 32 enzymes) #
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Alw44I 1570 GTGCAC G^TGCAC
1638 GTGCAC
Aor51HI 392 AGCGCT AGC^GCT
896 AGCGCT
ApaI 41 GGGCCC GGGCC^C
1946 GGGCCC
ApaLI 1570 GTGCAC G^TGCAC
1638 GTGCAC
AspI 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
880 GACCAGGTC
BbeI 631 GGCGCC GGCGC^C
1450 GGCGCC
BbsI 1408 GTCTTC GAAGAC(2/6)
1678 GTCTTC
BfaI 79 CTAG C^TAG
1379 CTAG
BpmI 753 CTCCAG CTGGAG(16/14)
1918 CTGGAG
BpuAI 1408 GTCTTC GAAGAC(2/6)
1678 GTCTTC
BsaI 682 GGTCTC GGTCTC(1/5)
1629 GGTCTC
BsiEI 402 CGGCCG CG[GA][CT]^CG
1254 CGACCG
BsmI 300 GAATGC GAATGC(1/-1)
1007 GCATTC
BspMI 383 ACCTGC ACCTGC(4/8)
791 ACCTGC
BsrFI 195 GCCGGT [GA]^CCGG[CT]
1249 GCCGGC
Cfr10I 195 GCCGGT [GA]^CCGG[CT]
1249 GCCGGC
DdeI 465 CTCAG C^T[ACGT]AG
1043 CTGAG
Eco47III 392 AGCGCT AGC^GCT
896 AGCGCT
EcoT14I 1217 CCATGG C^C[AT][AT]GG
1778 CCATGG
HgaI 176 GACGC GACGC(5/10)
966 GACGC
KasI 631 GGCGCC G^GCGCC
1450 GGCGCC
MaeI 79 CTAG C^TAG
1379 CTAG
NarI 631 GGCGCC GG^CGCC
1450 GGCGCC
NcoI 1217 CCATGG C^CATGG
1778 CCATGG
PflFI 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
880 GACCAGGTC
PpuMI 1712 AGGACCT [GA]G^G[AT]CC[CT]
1934 AGGACCC
SfoI 631 GGCGCC GGC^GCC
1450 GGCGCC
StyI 1217 CCATGG C^C[AT][AT]GG
1778 CCATGG
TfiI 290 GATTC G^A[AT]TC
1500 GATTC
Tsp45I 568 GTGAC ^GT[GC]AC
808 GTGAC
Tth111I 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
880 GACCAGGTC
XcmI 458 CCAAGATCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
971 CCATGTTTCTCATGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcgI BcnI
BmyI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SspBI TaqI
TseI TspRI TthHB8I Van91I XhoII