(Linear) (Six-base) MAPSORT of: KIEE2739.seq Check: 4690 from: 1 to: 2016 KIEE2739.orf With 182 enzymes: SgfI * December 9, 2018 22:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 800 2016 Size: 800 1216 Acc65I G'GTAC_C Cuts at: 0 953 2016 Size: 953 1063 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 979 1665 2016 Size: 979 686 351 Fragments arranged by size: 979 686 351 AfeI AGC'GCT Cuts at: 0 394 898 2016 Size: 394 504 1118 Fragments arranged by size: 1118 504 394 AflIII A'CryG_T Cuts at: 0 473 2016 Size: 473 1543 AleI CACnn'nnGTG Cuts at: 0 1072 2016 Size: 1072 944 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1549 2016 Size: 1549 467 AlwNI CAG_nnn'CTG Cuts at: 0 259 384 507 761 1830 2016 Size: 259 125 123 254 1069 186 Fragments arranged by size: 1069 259 254 186 125 123 ApaI G_GGCC'C Cuts at: 0 45 1950 2016 Size: 45 1905 66 Fragments arranged by size: 1905 66 45 ApaLI G'TGCA_C Cuts at: 0 1570 1638 2016 Size: 1570 68 378 Fragments arranged by size: 1570 378 68 AvaI C'yCGr_G Cuts at: 0 858 1504 1521 1938 2016 Size: 858 646 17 417 78 Fragments arranged by size: 858 646 417 78 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 331 364 2016 Size: 331 33 1652 Fragments arranged by size: 1652 331 33 BamHI G'GATC_C Cuts at: 0 732 2016 Size: 732 1284 BanI G'GyrC_C Cuts at: 0 27 631 800 953 1038 1389 1450 1763 Size: 27 604 169 153 85 351 61 313 Cuts at: 1763 1806 2016 Size: 43 210 Fragments arranged by size: 604 351 313 210 169 153 85 61 43 27 BanII G_rGCy'C Cuts at: 0 45 1796 1950 2016 Size: 45 1751 154 66 Fragments arranged by size: 1751 154 66 45 BbeI G_GCGC'C Cuts at: 0 635 1454 2016 Size: 635 819 562 Fragments arranged by size: 819 635 562 BbsI GAAGACnn'nnnn_ Cuts at: 0 1401 1671 2016 Size: 1401 270 345 Fragments arranged by size: 1401 345 270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 317 378 412 695 729 2016 Size: 283 34 61 34 283 34 1287 Fragments arranged by size: 1287 283 283 61 34 34 34 BclI T'GATC_A Cuts at: 0 998 2016 Size: 998 1018 BglI GCCn_nnn'nGGC Cuts at: 0 49 2016 Size: 49 1967 BglII A'GATC_T Cuts at: 0 461 2016 Size: 461 1555 Bme1580I G_kGCm'C Cuts at: 0 45 1552 1574 1642 1768 1950 2016 Size: 45 1507 22 68 126 182 66 Fragments arranged by size: 1507 182 126 68 66 45 22 BmrI ACTGGGnnnn_n' Cuts at: 0 334 2016 Size: 334 1682 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 39 2016 Size: 39 1977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 738 1939 2016 Size: 738 1201 77 Fragments arranged by size: 1201 738 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 688 1635 2016 Size: 688 947 381 Fragments arranged by size: 947 688 381 BsaHI Gr'CG_yC Cuts at: 0 632 967 1451 2016 Size: 632 335 484 565 Fragments arranged by size: 632 565 484 335 BsaWI w'CCGG_w Cuts at: 0 481 2016 Size: 481 1535 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 463 493 2016 Size: 463 30 1523 Fragments arranged by size: 1523 463 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 829 1606 1731 1906 2016 Size: 19 810 777 125 175 110 Fragments arranged by size: 810 777 175 125 110 19 BseYI C'CCAG_C Cuts at: 0 1551 1943 2016 Size: 1551 392 73 Fragments arranged by size: 1551 392 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 894 913 1071 1351 1555 2016 Size: 153 741 19 158 280 204 461 Fragments arranged by size: 741 461 280 204 158 153 19 BsiEI CG_ry'CG Cuts at: 0 405 1257 2016 Size: 405 852 759 Fragments arranged by size: 852 759 405 BsiHKAI G_wGCw'C Cuts at: 0 11 434 1574 1642 2016 Size: 11 423 1140 68 374 Fragments arranged by size: 1140 423 374 68 11 BsmI GAATG_Cn' Cuts at: 0 306 1007 2016 Size: 306 701 1009 Fragments arranged by size: 1009 701 306 BsmBI CGTCTCn'nnnn_ Cuts at: 0 861 2016 Size: 861 1155 Bsp1286I G_dGCh'C Cuts at: 0 11 45 434 1552 1574 1642 1768 1796 Size: 11 34 389 1118 22 68 126 28 Cuts at: 1796 1950 2016 Size: 154 66 Fragments arranged by size: 1118 389 154 126 68 66 34 28 22 11 BspEI T'CCGG_A Cuts at: 0 481 2016 Size: 481 1535 BspHI T'CATG_A Cuts at: 0 1976 2016 Size: 1976 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 392 800 2016 Size: 392 408 1216 Fragments arranged by size: 1216 408 392 BsrDI GCAATG_nn' Cuts at: 0 83 2016 Size: 83 1933 BsrFI r'CCGG_y Cuts at: 0 195 1249 2016 Size: 195 1054 767 Fragments arranged by size: 1054 767 195 BsrGI T'GTAC_A Cuts at: 0 446 593 1752 2016 Size: 446 147 1159 264 Fragments arranged by size: 1159 446 264 147 BssSI C'ACGA_G Cuts at: 0 661 2016 Size: 661 1355 BstXI CCAn_nnnn'nTGG Cuts at: 0 1917 2016 Size: 1917 99 BstYI r'GATC_y Cuts at: 0 461 732 816 2016 Size: 461 271 84 1200 Fragments arranged by size: 1200 461 271 84 Bsu36I CC'TnA_GG Cuts at: 0 1043 2016 Size: 1043 973 BtgI C'CryG_G Cuts at: 0 740 823 1099 1217 1325 1778 2016 Size: 740 83 276 118 108 453 238 Fragments arranged by size: 740 453 276 238 118 108 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 519 1074 2016 Size: 519 555 942 Fragments arranged by size: 942 555 519 BtsI GCAGTG_nn' Cuts at: 0 237 1991 2016 Size: 237 1754 25 Fragments arranged by size: 1754 237 25 DraIII CAC_nnn'GTG Cuts at: 0 243 666 807 2016 Size: 243 423 141 1209 Fragments arranged by size: 1209 423 243 141 DrdI GACnn_nn'nnGTC Cuts at: 0 79 2016 Size: 79 1937 EaeI y'GGCC_r Cuts at: 0 193 234 402 581 905 1160 1240 1988 Size: 193 41 168 179 324 255 80 748 Cuts at: 1988 2016 Size: 28 Fragments arranged by size: 748 324 255 193 179 168 80 41 28 EagI C'GGCC_G Cuts at: 0 402 2016 Size: 402 1614 EarI CTCTTCn'nnn_ Cuts at: 0 217 313 691 907 1216 1360 1429 1822 Size: 217 96 378 216 309 144 69 393 Cuts at: 1822 2016 Size: 194 Fragments arranged by size: 393 378 309 217 216 194 144 96 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 403 1392 1810 1865 2016 Size: 403 989 418 55 151 Fragments arranged by size: 989 418 403 151 55 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 738 979 1665 1939 2016 Size: 738 241 686 274 77 Fragments arranged by size: 738 686 274 241 77 EcoNI CCTnn'n_nnAGG Cuts at: 0 1885 2016 Size: 1885 131 EcoO109I rG'GnC_Cy Cuts at: 0 41 51 203 1473 1713 1800 1935 2016 Size: 41 10 152 1270 240 87 135 81 Fragments arranged by size: 1270 240 152 135 87 81 41 10 FspI TGC'GCA Cuts at: 0 388 2016 Size: 388 1628 HaeII r_GCGC'y Cuts at: 0 266 396 635 900 1423 1454 2016 Size: 266 130 239 265 523 31 562 Fragments arranged by size: 562 523 266 265 239 130 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 48 80 463 495 801 833 836 868 Size: 48 32 383 32 306 32 3 32 Cuts at: 868 2016 Size: 1148 Fragments arranged by size: 1148 383 306 48 32 32 32 32 3 HincII GTy'rAC Cuts at: 0 1959 2016 Size: 1959 57 KasI G'GCGC_C Cuts at: 0 631 1450 2016 Size: 631 819 566 Fragments arranged by size: 819 631 566 KpnI G_GTAC'C Cuts at: 0 957 2016 Size: 957 1059 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1109 2016 Size: 1109 907 MscI TGG'CCA Cuts at: 0 236 2016 Size: 236 1780 MslI CAynn'nnrTG Cuts at: 0 370 553 643 985 1072 1783 2016 Size: 370 183 90 342 87 711 233 Fragments arranged by size: 711 370 342 233 183 90 87 MspA1I CmG'CkG Cuts at: 0 358 1101 1529 1900 2016 Size: 358 743 428 371 116 Fragments arranged by size: 743 428 371 358 116 NaeI GCC'GGC Cuts at: 0 1251 2016 Size: 1251 765 NarI GG'CG_CC Cuts at: 0 632 1451 2016 Size: 632 819 565 Fragments arranged by size: 819 632 565 NcoI C'CATG_G Cuts at: 0 1217 1778 2016 Size: 1217 561 238 Fragments arranged by size: 1217 561 238 NgoMIV G'CCGG_C Cuts at: 0 1249 2016 Size: 1249 767 NotI GC'GGCC_GC Cuts at: 0 402 2016 Size: 402 1614 NspI r_CATG'y Cuts at: 0 477 2016 Size: 477 1539 PciI A'CATG_T Cuts at: 0 473 2016 Size: 473 1543 PflMI CCAn_nnn'nTGG Cuts at: 0 243 666 1674 2016 Size: 243 423 1008 342 Fragments arranged by size: 1008 423 342 243 PpuMI rG'GwC_Cy Cuts at: 0 1713 1935 2016 Size: 1713 222 81 Fragments arranged by size: 1713 222 81 PshAI GACnn'nnGTC Cuts at: 0 616 2016 Size: 616 1400 PspOMI G'GGCC_C Cuts at: 0 41 1946 2016 Size: 41 1905 70 Fragments arranged by size: 1905 70 41 PvuII CAG'CTG Cuts at: 0 1529 2016 Size: 1529 487 SacII CC_GC'GG Cuts at: 0 1102 2016 Size: 1102 914 SapI GCTCTTCn'nnn_ Cuts at: 0 313 907 1216 1360 1429 2016 Size: 313 594 309 144 69 587 Fragments arranged by size: 594 587 313 309 144 69 SexAI A'CCwGG_T Cuts at: 0 881 2016 Size: 881 1135 SfiI GGCCn_nnn'nGGCC Cuts at: 0 49 2016 Size: 49 1967 SfoI GGC'GCC Cuts at: 0 633 1452 2016 Size: 633 819 564 Fragments arranged by size: 819 633 564 SgrAI Cr'CCGG_yG Cuts at: 0 1249 2016 Size: 1249 767 StyI C'CwwG_G Cuts at: 0 1217 1778 2016 Size: 1217 561 238 Fragments arranged by size: 1217 561 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1275 1751 2016 Size: 1275 476 265 Fragments arranged by size: 1275 476 265 TatI w'GTAC_w Cuts at: 0 446 593 1752 2016 Size: 446 147 1159 264 Fragments arranged by size: 1159 446 264 147 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 808 840 2016 Size: 808 32 1176 Fragments arranged by size: 1176 808 32 Tth111I GACn'n_nGTC Cuts at: 0 723 883 2016 Size: 723 160 1133 Fragments arranged by size: 1133 723 160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 465 978 2016 Size: 465 513 1038 Fragments arranged by size: 1038 513 465 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmrI BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PflMI PpuMI PshAI PspOMI PvuII SacII SapI SexAI SfiI SfoI SgrAI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BbvCI BciVI BfrBI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsiWI BsrBI BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI ZraI