Restriction map for
pFN21AE2402
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1958 GTCTAC GT^[AC][GT]AC
AcsI 316 AAATTT [GA]^AATT[CT]
AfaI 341 GTAC GT^AC
AocI 2053 CCTGAGG CC^T[ACGT]AGG
ApoI 316 AAATTT [GA]^AATT[CT]
Asp700I 177 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1255 GGATCC G^GATCC
BbeI 517 GGCGCC GGCGC^C
BbrPI 1198 CACGTG CAC^GTG
BbsI 1294 GTCTTC GAAGAC(2/6)
BpmI 1189 CTGGAG CTGGAG(16/14)
BpuAI 1294 GTCTTC GAAGAC(2/6)
BsaAI 1198 CACGTG [CT]AC^GT[GA]
BsaI 1879 GAGACC GGTCTC(1/5)
BsgI 441 CTGCAC GTGCAG(16/14)
Bsp1407I 340 TGTACA T^GTACA
BsrFI 1400 GCCGGC [GA]^CCGG[CT]
BsrGI 340 TGTACA T^GTACA
BssHII 94 GCGCGC G^CGCGC
BstAPI 1197 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2053 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1400 GCCGGC [GA]^CCGG[CT]
DraIII 1198 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2053 CCTGAGG CC^T[ACGT]AGG
EcoNI 711 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 2003 AAGCTT A^AGCTT
KasI 517 GGCGCC G^GCGCC
KspI 1312 CCGCGG CCGC^GG
MseI 1634 TTAA T^TAA
MslI 252 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1400 GCCGGC GCC^GGC
NarI 517 GGCGCC GG^CGCC
NgoMIV 1400 GCCGGC G^CCGGC
NspI 1331 GCATGC [GA]CATG^[CT]
PmaCI 1198 CACGTG CAC^GTG
PmlI 1198 CACGTG CAC^GTG
PpuMI 1832 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 341 GTAC GT^AC
SacII 1312 CCGCGG CCGC^GG
SbfI 1527 CCTGCAGG CCTGCA^GG
SfaNI 1756 GATGC GCATC(5/9)
SfoI 517 GGCGCC GGC^GCC
SmlI 2071 CTCAAG C^T[CT][GA]AG
SphI 1331 GCATGC GCATG^C
Sse8387I 1527 CCTGCAGG CCTGCA^GG
SspBI 340 TGTACA T^GTACA
StuI 1524 AGGCCT AGG^CCT
Tru9I 1634 TTAA T^TAA
Tsp509I 317 AATT ^AATT
XmnI 177 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 228 GTGCAC G^TGCAC
1888 GTGCAC
AlwNI 943 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1531 CAGGAGCTG
ApaLI 228 GTGCAC G^TGCAC
1888 GTGCAC
BbvCI 1467 CCTCAGC CCTCAGC(-5/-2)
391 GCTGAGG
BfaI 919 CTAG C^TAG
1969 CTAG
BglI 597 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2011 GCCCGGCTGGC
BlpI 1580 GCTCAGC GC^T[ACGT]AGC
1794 GCTGAGC
Bpu1102I 1580 GCTCAGC GC^T[ACGT]AGC
1794 GCTGAGC
BsmBI 1768 CGTCTC CGTCTC(1/5)
728 GAGACG
BspMI 1973 ACCTGC ACCTGC(4/8)
1738 GCAGGT
BsrBI 531 GAGCGG CCGCTC(-3/-3)
1192 GAGCGG
BsrI 842 ACTGG ACTGG(1/-1)
1838 CCAGT
BstYI 413 AGATCC [GA]^GATC[CT]
1255 GGATCC
CelII 1580 GCTCAGC GC^T[ACGT]AGC
1794 GCTGAGC
EaeI 486 TGGCCG [CT]^GGCC[GA]
1063 CGGCCA
EcoT14I 676 CCAAGG C^C[AT][AT]GG
1244 CCTTGG
HaeII 517 GGCGCC [GA]GCGC^[CT]
1798 AGCGCC
MaeI 919 CTAG C^TAG
1969 CTAG
MaeIII 1410 GTCAC ^GT[ACGT]AC
1781 GTCAC
MflI 413 AGATCC [GA]^GATC[CT]
1255 GGATCC
PflMI 419 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
942 CCAGCTGCTGG
PshAI 1369 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1476 GACAGGTGTC
StyI 676 CCAAGG C^C[AT][AT]GG
1244 CCTTGG
Tsp45I 1410 GTCAC ^GT[GC]AC
1781 GTCAC
Van91I 419 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
942 CCAGCTGCTGG
XhoII 413 AGATCC [GA]^GATC[CT]
1255 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcgI BcnI BmyI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII Sau3AI
Sau96I ScrFI SfcI SmaI TaqI
TfiI TseI TspRI TthHB8I XmaCI
XmaI