(Circular) (Six-base) MAPSORT of: pFN21AE2402.seq Check: 1782 from: 1 to: 6787 pFN21AE2402 6787 bp With 182 enzymes: SgfI * February 28, 2012 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4597 278 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3964 4130 1937 Size: 2027 166 4594 Fragments arranged by size: 4594 2027 166 Acc65I G'GTAC_C Cuts at: 4108 4108 Size: 6787 AclI AA'CG_TT Cuts at: 1563 4914 5287 1563 Size: 3351 373 3063 Fragments arranged by size: 3351 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2402 2738 4850 6234 6502 1632 Size: 770 336 2112 1384 268 1917 Fragments arranged by size: 2112 1917 1384 770 336 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6590 AflIII A'CryG_T Cuts at: 1203 5686 6663 1203 Size: 4483 977 1327 Fragments arranged by size: 4483 1327 977 AgeI A'CCGG_T Cuts at: 5618 5618 Size: 6787 AhdI GACnn_n'nnGTC Cuts at: 5516 5516 Size: 6787 AleI CACnn'nnGTG Cuts at: 1166 2261 1166 Size: 1095 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2253 1184 Size: 1069 5718 AlwNI CAG_nnn'CTG Cuts at: 2953 3541 4530 6102 2953 Size: 588 989 1572 3638 Fragments arranged by size: 3638 1572 989 588 ApaI G_GGCC'C Cuts at: 2043 2199 2597 2613 2637 2888 3144 3167 4038 Size: 156 398 16 24 251 256 23 871 Cuts at: 4038 2043 Size: 4792 Fragments arranged by size: 4792 871 398 256 251 156 24 23 16 ApaLI G'TGCA_C Cuts at: 2233 3893 4844 6000 2233 Size: 1660 951 1156 3020 Fragments arranged by size: 3020 1660 1156 951 ApoI r'AATT_y Cuts at: 1493 2321 4096 4322 4474 6583 1493 Size: 828 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 828 226 152 AseI AT'TA_AT Cuts at: 160 1032 5341 160 Size: 872 4309 1606 Fragments arranged by size: 4309 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 AvaI C'yCGr_G Cuts at: 1870 1943 2198 2770 2840 3075 3279 3311 4112 Size: 73 255 572 70 235 204 32 801 Cuts at: 4112 1870 Size: 4545 Fragments arranged by size: 4545 801 572 255 235 204 73 70 32 BamHI G'GATC_C Cuts at: 3260 4117 4484 3260 Size: 857 367 5563 Fragments arranged by size: 5563 857 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2522 2712 2893 2962 4108 Size: 325 217 627 735 190 181 69 1146 Cuts at: 4108 5563 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1146 735 627 325 217 190 181 69 BanII G_rGCy'C Cuts at: 1391 2043 2199 2597 2613 2637 2835 2888 3144 Size: 652 156 398 16 24 198 53 256 Cuts at: 3144 3167 3237 3587 4038 4106 1391 Size: 23 70 350 451 68 4072 Fragments arranged by size: 4072 652 451 398 350 256 198 156 70 68 53 24 23 16 BbeI G_GCGC'C Cuts at: 2526 2526 Size: 6787 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3292 928 Size: 971 1393 4423 Fragments arranged by size: 4423 1393 971 BbvCI CC'TCA_GC Cuts at: 2397 3473 2397 Size: 1076 5711 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2100 2133 2134 2167 2344 2378 3995 Size: 34 163 33 1 33 177 34 1617 Cuts at: 3995 4029 4978 5012 1903 Size: 34 949 34 3678 Fragments arranged by size: 3678 1617 949 177 163 34 34 34 34 33 33 1 BciVI GTATCCnnnnn_n' Cuts at: 4679 5889 4679 Size: 1210 5577 BclI T'GATC_A Cuts at: 1548 1548 Size: 6787 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2608 4022 5398 Size: 107 122 71 631 605 936 1414 1376 Cuts at: 5398 136 Size: 1525 Fragments arranged by size: 1525 1414 1376 936 631 605 122 107 71 BglII A'GATC_T Cuts at: 6782 6782 Size: 6787 BlpI GC'TnA_GC Cuts at: 3586 3800 4204 3586 Size: 214 404 6169 Fragments arranged by size: 6169 404 214 Bme1580I G_kGCm'C Cuts at: 1790 2043 2199 2237 2441 2597 2613 2637 2888 Size: 253 156 38 204 156 16 24 251 Cuts at: 2888 3020 3144 3167 3466 3897 4018 4038 4848 Size: 132 124 23 299 431 121 20 810 Cuts at: 4848 6004 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 810 431 299 253 251 204 156 156 132 124 121 38 24 23 20 16 BmgBI CAC'GTC Cuts at: 1318 2626 3832 3870 1318 Size: 1308 1206 38 4235 Fragments arranged by size: 4235 1308 1206 38 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2857 3837 4518 Size: 416 53 83 399 24 1440 980 681 Cuts at: 4518 5476 442 Size: 958 1753 Fragments arranged by size: 1753 1440 980 958 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3215 5447 1239 Size: 1976 2232 2579 Fragments arranged by size: 2579 2232 1976 Bpu10I CC'TnA_GC Cuts at: 2397 3231 3401 3473 3815 2397 Size: 834 170 72 342 5369 Fragments arranged by size: 5369 834 342 170 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4061 4912 5777 6075 4061 Size: 851 865 298 4773 Fragments arranged by size: 4773 865 851 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3878 5450 882 Size: 617 2379 1572 2219 Fragments arranged by size: 2379 2219 1572 617 BsaAI yAC'GTr Cuts at: 493 1204 3205 4578 493 Size: 711 2001 1373 2702 Fragments arranged by size: 2702 2001 1373 711 BsaBI GATnn'nnATC Cuts at: 6781 6781 Size: 6787 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2107 2523 2736 Size: 53 83 186 936 524 50 416 213 Cuts at: 2736 4594 4976 5611 275 Size: 1858 382 635 1451 Fragments arranged by size: 1858 1451 936 635 524 416 382 213 186 83 53 50 BsaWI w'CCGG_w Cuts at: 5220 5618 5892 6039 6450 5220 Size: 398 274 147 411 5557 Fragments arranged by size: 5557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2981 2987 3011 3017 3968 Size: 30 165 30 1578 6 24 6 951 Cuts at: 3968 3998 1178 Size: 30 3967 Fragments arranged by size: 3967 1578 951 165 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2531 2570 2735 2965 2974 3845 4076 1186 Size: 1345 39 165 230 9 871 231 3897 Fragments arranged by size: 3897 1345 871 231 230 165 39 9 BseYI C'CCAG_C Cuts at: 2285 2430 2887 3166 3648 3795 5990 6486 2285 Size: 145 457 279 482 147 2195 496 2586 Fragments arranged by size: 2586 2195 496 482 457 279 147 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2431 1163 Size: 573 695 5519 Fragments arranged by size: 5519 695 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4237 4998 6026 1540 Size: 275 186 2236 761 1028 2301 Fragments arranged by size: 2301 2236 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2250 3549 3897 4848 4933 6004 2237 Size: 13 1299 348 951 85 1071 3020 Fragments arranged by size: 3020 1299 1071 951 348 85 13 BsmI GAATG_Cn' Cuts at: 4298 4391 4298 Size: 93 6694 BsmBI CGTCTCn'nnnn_ Cuts at: 2727 3779 2727 Size: 1052 5735 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2199 2237 2250 2441 2597 2613 Size: 399 253 156 38 13 191 156 16 Cuts at: 2613 2637 2835 2888 3020 3144 3167 3237 3466 Size: 24 198 53 132 124 23 70 229 Cuts at: 3466 3549 3587 3897 4018 4038 4106 4848 4933 Size: 83 38 310 121 20 68 742 85 Cuts at: 4933 6004 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 310 253 229 198 191 156 156 132 124 121 85 83 70 68 53 38 38 24 23 20 16 13 BspEI T'CCGG_A Cuts at: 6450 6450 Size: 6787 BspHI T'CATG_A Cuts at: 4676 6406 4676 Size: 1730 5057 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 3987 4142 844 Size: 2890 253 155 3489 Fragments arranged by size: 3489 2890 253 155 BsrBI CCG'CTC Cuts at: 1394 1823 2538 3199 4674 1394 Size: 429 715 661 1475 3507 Fragments arranged by size: 3507 1475 715 661 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5282 5456 65 Size: 1183 4034 174 1396 Fragments arranged by size: 4034 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3405 5431 5618 1700 Size: 110 1595 2026 187 2869 Fragments arranged by size: 2869 2026 1595 187 110 BsrGI T'GTAC_A Cuts at: 96 2345 96 Size: 2249 4538 BssHII G'CGCG_C Cuts at: 1925 2099 1925 Size: 174 6613 BssSI C'ACGA_G Cuts at: 4847 5859 4847 Size: 1012 5775 BstAPI GCAn_nnn'nTGC Cuts at: 794 3208 794 Size: 2414 4373 BstBI TT'CG_AA Cuts at: 5591 5591 Size: 6787 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6787 BstYI r'GATC_y Cuts at: 1972 2418 3260 4117 4484 4869 4886 6338 6424 Size: 446 842 857 367 385 17 1452 86 Cuts at: 6424 6436 6782 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 857 842 446 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4059 4059 Size: 6787 BtgI C'CryG_G Cuts at: 513 1065 2004 3103 3317 3706 513 Size: 552 939 1099 214 389 3594 Fragments arranged by size: 3594 1099 939 552 389 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3006 487 Size: 683 56 1780 4268 Fragments arranged by size: 4268 1780 683 56 BtsI GCAGTG_nn' Cuts at: 2235 2237 3139 3593 3820 3931 4303 5097 5117 Size: 2 902 454 227 111 372 794 20 Cuts at: 5117 2235 Size: 3905 Fragments arranged by size: 3905 902 794 454 372 227 111 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6787 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6752 DraI TTT'AAA Cuts at: 4091 4091 Size: 6787 DraIII CAC_nnn'GTG Cuts at: 3208 3208 Size: 6787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5794 809 Size: 507 4478 1802 Fragments arranged by size: 4478 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2491 3068 4234 Size: 54 1425 50 275 18 661 577 1166 Cuts at: 4234 5123 6752 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1166 889 661 577 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4234 1537 Size: 275 2422 4090 Fragments arranged by size: 4090 2422 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 2983 3016 4717 1353 Size: 957 673 33 1701 3423 Fragments arranged by size: 3423 1701 957 673 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3424 3938 5374 5748 5894 387 Size: 1064 138 1835 514 1436 374 146 1280 Fragments arranged by size: 1835 1436 1280 1064 514 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 2738 3215 4850 5447 6234 6502 Size: 393 770 336 477 1635 597 787 268 Cuts at: 6502 1239 Size: 1524 Fragments arranged by size: 1635 1524 787 770 597 477 393 336 268 EcoNI CCTnn'n_nnAGG Cuts at: 2720 2720 Size: 6787 EcoO109I rG'GnC_Cy Cuts at: 2040 2195 2486 2593 2594 2610 2634 2884 3140 Size: 155 291 107 1 16 24 250 256 Cuts at: 3140 3141 3838 4035 2040 Size: 1 697 197 4792 Fragments arranged by size: 4792 697 291 256 250 197 155 107 24 16 1 1 EcoRI G'AATT_C Cuts at: 4096 4474 4096 Size: 378 6409 FspI TGC'GCA Cuts at: 5293 5293 Size: 6787 HaeII r_GCGC'y Cuts at: 1678 2526 3807 5934 1678 Size: 848 1281 2127 2531 Fragments arranged by size: 2531 2127 1281 848 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2960 2981 2987 2992 2993 Size: 32 135 32 1388 21 6 5 1 Cuts at: 2993 3013 3019 3025 3694 3726 3968 4000 5428 Size: 20 6 6 669 32 242 32 1428 Cuts at: 5428 5460 5502 5534 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1428 1388 669 242 135 42 32 32 32 32 32 32 21 20 6 6 6 5 1 HincII GTy'rAC Cuts at: 669 1657 1938 4131 4377 669 Size: 988 281 2193 246 3079 Fragments arranged by size: 3079 2193 988 281 246 HindIII A'AGCT_T Cuts at: 748 4008 748 Size: 3260 3527 HpaI GTT'AAC Cuts at: 4377 4377 Size: 6787 KasI G'GCGC_C Cuts at: 2522 2522 Size: 6787 KpnI G_GTAC'C Cuts at: 4112 4112 Size: 6787 MfeI C'AATT_G Cuts at: 4386 4386 Size: 6787 MluI A'CGCG_T Cuts at: 6663 6663 Size: 6787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 3030 3288 5901 6085 1305 Size: 1719 6 258 2613 184 2007 Fragments arranged by size: 2613 2007 1719 258 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4965 Fragments arranged by size: 4965 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2261 4745 5104 5263 518 Size: 648 843 252 2484 359 159 2042 Fragments arranged by size: 2484 2042 843 648 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2118 2127 2261 2891 2950 3153 3193 3319 Size: 512 9 134 630 59 203 40 126 Cuts at: 3319 3363 3477 3624 3799 3943 3954 4028 4053 Size: 44 114 147 175 144 11 74 25 Cuts at: 4053 4203 4880 6028 6273 1606 Size: 150 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 512 245 203 175 150 147 144 134 126 114 74 59 44 40 25 11 9 NaeI GCC'GGC Cuts at: 3407 3407 Size: 6787 NarI GG'CG_CC Cuts at: 2523 2523 Size: 6787 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5296 Fragments arranged by size: 5296 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6787 NgoMIV G'CCGG_C Cuts at: 3405 3405 Size: 6787 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6787 NotI GC'GGCC_GC Cuts at: 4234 4234 Size: 6787 NspI r_CATG'y Cuts at: 1132 1229 3340 4145 5690 1132 Size: 97 2111 805 1545 2229 Fragments arranged by size: 2229 2111 1545 805 97 PasI CC'CwG_GG Cuts at: 1348 2787 2828 3160 3580 1348 Size: 1439 41 332 420 4555 Fragments arranged by size: 4555 1439 420 332 41 PciI A'CATG_T Cuts at: 5686 5686 Size: 6787 PflMI CCAn_nnn'nTGG Cuts at: 1108 2430 2953 1108 Size: 1322 523 4942 Fragments arranged by size: 4942 1322 523 PfoI T'CCnGG_A Cuts at: 3612 3654 3612 Size: 42 6745 PmeI GTTT'AAAC Cuts at: 4091 4091 Size: 6787 PmlI CAC'GTG Cuts at: 3205 3205 Size: 6787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4856 4888 6369 6401 4856 Size: 32 1481 32 5242 Fragments arranged by size: 5242 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3838 3838 Size: 6787 PshAI GACnn'nnGTC Cuts at: 1613 3378 3485 1613 Size: 1765 107 4915 Fragments arranged by size: 4915 1765 107 PsiI TTA'TAA Cuts at: 4357 4357 Size: 6787 PspOMI G'GGCC_C Cuts at: 2039 2195 2593 2609 2633 2884 3140 3163 4034 Size: 156 398 16 24 251 256 23 871 Cuts at: 4034 2039 Size: 4792 Fragments arranged by size: 4792 871 398 256 251 156 24 23 16 PstI C_TGCA'G Cuts at: 830 2881 3537 3720 4139 830 Size: 2051 656 183 419 3478 Fragments arranged by size: 3478 2051 656 419 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6787 PvuII CAG'CTG Cuts at: 2261 2950 3153 3193 3477 3799 4028 2261 Size: 689 203 40 284 322 229 5020 Fragments arranged by size: 5020 689 322 284 229 203 40 SacII CC_GC'GG Cuts at: 3320 3320 Size: 6787 SalI G'TCGA_C Cuts at: 1936 4129 1936 Size: 2193 4594 SanDI GG'GwC_CC Cuts at: 3838 3838 Size: 6787 SbfI CC_TGCA'GG Cuts at: 3537 4139 3537 Size: 602 6185 ScaI AGT'ACT Cuts at: 5035 6747 5035 Size: 1712 5075 SfcI C'TryA_G Cuts at: 826 1045 2877 3533 3716 4135 5270 5951 6142 Size: 219 1832 656 183 419 1135 681 191 Cuts at: 6142 826 Size: 1471 Fragments arranged by size: 1832 1471 1135 681 656 419 219 191 183 SfoI GGC'GCC Cuts at: 2524 2524 Size: 6787 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6787 SmaI CCC'GGG Cuts at: 1872 2200 2772 3077 3281 4114 1872 Size: 328 572 305 204 833 4545 Fragments arranged by size: 4545 833 572 328 305 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 4076 4891 5792 6054 820 Size: 197 926 2133 815 901 262 1553 Fragments arranged by size: 2133 1553 926 901 815 262 197 SnaBI TAC'GTA Cuts at: 493 4578 493 Size: 4085 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6787 SphI G_CATG'C Cuts at: 1132 3340 4145 1132 Size: 2208 805 3774 Fragments arranged by size: 3774 2208 805 SrfI GCCC'GGGC Cuts at: 2200 2772 2200 Size: 572 6215 SspI AAT'ATT Cuts at: 5 52 4711 5 Size: 47 4659 2081 Fragments arranged by size: 4659 2081 47 StuI AGG'CCT Cuts at: 1517 3531 1517 Size: 2014 4773 StyI C'CwwG_G Cuts at: 513 1065 2004 2681 3249 4226 513 Size: 552 939 677 568 977 3074 Fragments arranged by size: 3074 977 939 677 568 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 2717 4814 4831 4984 5169 5614 Size: 1255 885 252 2097 17 153 185 445 Cuts at: 5614 325 Size: 1498 Fragments arranged by size: 2097 1498 1255 885 445 252 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2345 5033 6745 Size: 274 80 33 51 1443 368 2688 1712 Cuts at: 6745 96 Size: 138 Fragments arranged by size: 2688 1712 1443 368 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6747 6779 6747 Size: 32 6755 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6787 XbaI T'CTAG_A Cuts at: 4123 4123 Size: 6787 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6787 XmaI C'CCGG_G Cuts at: 1870 2198 2770 3075 3279 4112 1870 Size: 328 572 305 204 833 4545 Fragments arranged by size: 4545 833 572 328 305 204 XmnI GAAnn'nnTTC Cuts at: 2186 4916 2186 Size: 2730 4057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4595 276 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SwaI XcmI