(Linear) (Six-base) MAPSORT of: KIEE2402.seq Check: 4668 from: 1 to: 2082 KIEE2402.orf With 182 enzymes: SgfI * February 28, 2012 13:56 .. AccI GT'mk_AC Cuts at: 0 1959 2082 Size: 1959 123 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 733 2082 Size: 397 336 1349 Fragments arranged by size: 1349 397 336 AleI CACnn'nnGTG Cuts at: 0 256 2082 Size: 256 1826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 248 2082 Size: 248 1834 AlwNI CAG_nnn'CTG Cuts at: 0 948 1536 2082 Size: 948 588 546 Fragments arranged by size: 948 588 546 ApaI G_GGCC'C Cuts at: 0 38 194 592 608 632 883 1139 1162 Size: 38 156 398 16 24 251 256 23 Cuts at: 1162 2033 2082 Size: 871 49 Fragments arranged by size: 871 398 256 251 156 49 38 24 23 16 ApaLI G'TGCA_C Cuts at: 0 228 1888 2082 Size: 228 1660 194 Fragments arranged by size: 1660 228 194 ApoI r'AATT_y Cuts at: 0 316 2082 Size: 316 1766 AvaI C'yCGr_G Cuts at: 0 193 765 835 1070 1274 1306 2082 Size: 193 572 70 235 204 32 776 Fragments arranged by size: 776 572 235 204 193 70 32 BamHI G'GATC_C Cuts at: 0 1255 2082 Size: 1255 827 BanI G'GyrC_C Cuts at: 0 517 707 888 957 2082 Size: 517 190 181 69 1125 Fragments arranged by size: 1125 517 190 181 69 BanII G_rGCy'C Cuts at: 0 38 194 592 608 632 830 883 1139 Size: 38 156 398 16 24 198 53 256 Cuts at: 1139 1162 1232 1582 2033 2082 Size: 23 70 350 451 49 Fragments arranged by size: 451 398 350 256 198 156 70 53 49 38 24 23 16 BbeI G_GCGC'C Cuts at: 0 521 2082 Size: 521 1561 BbsI GAAGACnn'nnnn_ Cuts at: 0 1287 2082 Size: 1287 795 BbvCI CC'TCA_GC Cuts at: 0 392 1468 2082 Size: 392 1076 614 Fragments arranged by size: 1076 614 392 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 95 128 129 162 339 373 1990 2024 Size: 95 33 1 33 177 34 1617 34 Cuts at: 2024 2082 Size: 58 Fragments arranged by size: 1617 177 95 58 34 34 33 33 1 BglI GCCn_nnn'nGGC Cuts at: 0 603 2017 2082 Size: 603 1414 65 Fragments arranged by size: 1414 603 65 BlpI GC'TnA_GC Cuts at: 0 1581 1795 2082 Size: 1581 214 287 Fragments arranged by size: 1581 287 214 Bme1580I G_kGCm'C Cuts at: 0 38 194 232 436 592 608 632 883 Size: 38 156 38 204 156 16 24 251 Cuts at: 883 1015 1139 1162 1461 1892 2013 2033 2082 Size: 132 124 23 299 431 121 20 49 Fragments arranged by size: 431 299 251 204 156 156 132 124 121 49 38 38 24 23 20 16 BmgBI CAC'GTC Cuts at: 0 621 1827 1865 2082 Size: 621 1206 38 217 Fragments arranged by size: 1206 621 217 38 BmrI ACTGGGnnnn_n' Cuts at: 0 852 1832 2082 Size: 852 980 250 Fragments arranged by size: 980 852 250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1210 2082 Size: 1210 872 Bpu10I CC'TnA_GC Cuts at: 0 392 1226 1396 1468 1810 2082 Size: 392 834 170 72 342 272 Fragments arranged by size: 834 392 342 272 170 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2056 2082 Size: 2056 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 1873 2082 Size: 1873 209 BsaAI yAC'GTr Cuts at: 0 1200 2082 Size: 1200 882 BsaHI Gr'CG_yC Cuts at: 0 52 102 518 731 2082 Size: 52 50 416 213 1351 Fragments arranged by size: 1351 416 213 52 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 976 982 1006 1012 1963 1993 2082 Size: 976 6 24 6 951 30 89 Fragments arranged by size: 976 951 89 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 526 565 730 960 969 1840 2071 2082 Size: 526 39 165 230 9 871 231 11 Fragments arranged by size: 871 526 231 230 165 39 11 9 BseYI C'CCAG_C Cuts at: 0 280 425 882 1161 1643 1790 2082 Size: 280 145 457 279 482 147 292 Fragments arranged by size: 482 457 292 280 279 147 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 426 2082 Size: 426 1656 BsiHKAI G_wGCw'C Cuts at: 0 232 245 1544 1892 2082 Size: 232 13 1299 348 190 Fragments arranged by size: 1299 348 232 190 13 BsmBI CGTCTCn'nnnn_ Cuts at: 0 722 1774 2082 Size: 722 1052 308 Fragments arranged by size: 1052 722 308 Bsp1286I G_dGCh'C Cuts at: 0 38 194 232 245 436 592 608 632 Size: 38 156 38 13 191 156 16 24 Cuts at: 632 830 883 1015 1139 1162 1232 1461 1544 Size: 198 53 132 124 23 70 229 83 Cuts at: 1544 1582 1892 2013 2033 2082 Size: 38 310 121 20 49 Fragments arranged by size: 310 229 198 191 156 156 132 124 121 83 70 53 49 38 38 38 24 23 20 16 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1729 1982 2082 Size: 1729 253 100 Fragments arranged by size: 1729 253 100 BsrBI CCG'CTC Cuts at: 0 533 1194 2082 Size: 533 661 888 Fragments arranged by size: 888 661 533 BsrFI r'CCGG_y Cuts at: 0 1400 2082 Size: 1400 682 BsrGI T'GTAC_A Cuts at: 0 340 2082 Size: 340 1742 BssHII G'CGCG_C Cuts at: 0 94 2082 Size: 94 1988 BstAPI GCAn_nnn'nTGC Cuts at: 0 1203 2082 Size: 1203 879 BstYI r'GATC_y Cuts at: 0 413 1255 2082 Size: 413 842 827 Fragments arranged by size: 842 827 413 Bsu36I CC'TnA_GG Cuts at: 0 2054 2082 Size: 2054 28 BtgI C'CryG_G Cuts at: 0 1098 1312 1701 2082 Size: 1098 214 389 381 Fragments arranged by size: 1098 389 381 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1001 2082 Size: 1001 1081 BtsI GCAGTG_nn' Cuts at: 0 230 232 1134 1588 1815 1926 2082 Size: 230 2 902 454 227 111 156 Fragments arranged by size: 902 454 230 227 156 111 2 DraIII CAC_nnn'GTG Cuts at: 0 1203 2082 Size: 1203 879 EaeI y'GGCC_r Cuts at: 0 486 1063 2082 Size: 486 577 1019 Fragments arranged by size: 1019 577 486 EarI CTCTTCn'nnn_ Cuts at: 0 305 978 1011 2082 Size: 305 673 33 1071 Fragments arranged by size: 1071 673 305 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1419 1933 2082 Size: 1419 514 149 Fragments arranged by size: 1419 514 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 733 1210 2082 Size: 397 336 477 872 Fragments arranged by size: 872 477 397 336 EcoNI CCTnn'n_nnAGG Cuts at: 0 715 2082 Size: 715 1367 EcoO109I rG'GnC_Cy Cuts at: 0 35 190 481 588 589 605 629 879 Size: 35 155 291 107 1 16 24 250 Cuts at: 879 1135 1136 1833 2030 2082 Size: 256 1 697 197 52 Fragments arranged by size: 697 291 256 250 197 155 107 52 35 24 16 1 1 HaeII r_GCGC'y Cuts at: 0 521 1802 2082 Size: 521 1281 280 Fragments arranged by size: 1281 521 280 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 955 976 982 987 988 1008 1014 1020 Size: 955 21 6 5 1 20 6 6 Cuts at: 1020 1689 1721 1963 1995 2082 Size: 669 32 242 32 87 Fragments arranged by size: 955 669 242 87 32 32 21 20 6 6 6 5 1 HindIII A'AGCT_T Cuts at: 0 2003 2082 Size: 2003 79 KasI G'GCGC_C Cuts at: 0 517 2082 Size: 517 1565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1019 1025 1283 2082 Size: 1019 6 258 799 Fragments arranged by size: 1019 799 258 6 MslI CAynn'nnrTG Cuts at: 0 256 2082 Size: 256 1826 MspA1I CmG'CkG Cuts at: 0 113 122 256 886 945 1148 1188 1314 Size: 113 9 134 630 59 203 40 126 Cuts at: 1314 1358 1472 1619 1794 1938 1949 2023 2048 Size: 44 114 147 175 144 11 74 25 Cuts at: 2048 2082 Size: 34 Fragments arranged by size: 630 203 175 147 144 134 126 114 113 74 59 44 40 34 25 11 9 NaeI GCC'GGC Cuts at: 0 1402 2082 Size: 1402 680 NarI GG'CG_CC Cuts at: 0 518 2082 Size: 518 1564 NgoMIV G'CCGG_C Cuts at: 0 1400 2082 Size: 1400 682 NspI r_CATG'y Cuts at: 0 1335 2082 Size: 1335 747 PasI CC'CwG_GG Cuts at: 0 782 823 1155 1575 2082 Size: 782 41 332 420 507 Fragments arranged by size: 782 507 420 332 41 PflMI CCAn_nnn'nTGG Cuts at: 0 425 948 2082 Size: 425 523 1134 Fragments arranged by size: 1134 523 425 PfoI T'CCnGG_A Cuts at: 0 1607 1649 2082 Size: 1607 42 433 Fragments arranged by size: 1607 433 42 PmlI CAC'GTG Cuts at: 0 1200 2082 Size: 1200 882 PpuMI rG'GwC_Cy Cuts at: 0 1833 2082 Size: 1833 249 PshAI GACnn'nnGTC Cuts at: 0 1373 1480 2082 Size: 1373 107 602 Fragments arranged by size: 1373 602 107 PspOMI G'GGCC_C Cuts at: 0 34 190 588 604 628 879 1135 1158 Size: 34 156 398 16 24 251 256 23 Cuts at: 1158 2029 2082 Size: 871 53 Fragments arranged by size: 871 398 256 251 156 53 34 24 23 16 PstI C_TGCA'G Cuts at: 0 876 1532 1715 2082 Size: 876 656 183 367 Fragments arranged by size: 876 656 367 183 PvuII CAG'CTG Cuts at: 0 256 945 1148 1188 1472 1794 2023 2082 Size: 256 689 203 40 284 322 229 59 Fragments arranged by size: 689 322 284 256 229 203 59 40 SacII CC_GC'GG Cuts at: 0 1315 2082 Size: 1315 767 SanDI GG'GwC_CC Cuts at: 0 1833 2082 Size: 1833 249 SbfI CC_TGCA'GG Cuts at: 0 1532 2082 Size: 1532 550 SfcI C'TryA_G Cuts at: 0 872 1528 1711 2082 Size: 872 656 183 371 Fragments arranged by size: 872 656 371 183 SfoI GGC'GCC Cuts at: 0 519 2082 Size: 519 1563 SmaI CCC'GGG Cuts at: 0 195 767 1072 1276 2082 Size: 195 572 305 204 806 Fragments arranged by size: 806 572 305 204 195 SmlI C'TyrA_G Cuts at: 0 2071 2082 Size: 2071 11 SphI G_CATG'C Cuts at: 0 1335 2082 Size: 1335 747 SrfI GCCC'GGGC Cuts at: 0 195 767 2082 Size: 195 572 1315 Fragments arranged by size: 1315 572 195 StuI AGG'CCT Cuts at: 0 1526 2082 Size: 1526 556 StyI C'CwwG_G Cuts at: 0 676 1244 2082 Size: 676 568 838 Fragments arranged by size: 838 676 568 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 460 712 2082 Size: 460 252 1370 Fragments arranged by size: 1370 460 252 TatI w'GTAC_w Cuts at: 0 340 2082 Size: 340 1742 XmaI C'CCGG_G Cuts at: 0 193 765 1070 1274 2082 Size: 193 572 305 204 808 Fragments arranged by size: 808 572 305 204 193 XmnI GAAnn'nnTTC Cuts at: 0 181 2082 Size: 181 1901 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SbfI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI BplI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TstI Tth111I XbaI XcmI XhoI ZraI