Restriction map for
pFN21AE9670
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# NO CUT ENZYMES ( 43 enzymes) #
##################################
AccIII AscI AseI AsnI BamHI
BclI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DrdI EcoRI EcoT22I
FbaI KspI MluI MroI NdeI
NheI NotI NsiI PacI PmeI
PshBI PvuI RsrII SacII SalI
SbfI SfiI SnaBI Sse8387I SspBI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 9566 GACGTC GACGT^C
AccI 4905 GTCTAC GT^[AC][GT]AC
AccII 2401 CGCG CG^CG
AclI 2060 AACGTT AA^CGTT
AflII 6102 CTTAAG C^TTAAG
AflIII 4596 ACATGT A^C[GA][CT]GT
Alw44I 3715 GTGCAC G^TGCAC
ApaLI 3715 GTGCAC G^TGCAC
Asp700I 578 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 7321 CCTAGG C^CTAGG
BbeI 3048 GGCGCC GGCGC^C
BbrPI 4204 CACGTG CAC^GTG
BcgI 9356 CGACCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6102 CTTAAG C^TTAAG
BglII 7749 AGATCT A^GATCT
BlnI 7321 CCTAGG C^CTAGG
BsaAI 4204 CACGTG [CT]AC^GT[GA]
BsaBI 2800 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4596 ACATGT A^CATGT
BsrBI 5489 CCGCTC CCGCTC(-3/-3)
BstBI 8240 TTCGAA TT^CGAA
BstUI 2401 CGCG CG^CG
DraI 2077 TTTAAA TTT^AAA
EagI 5399 CGGCCG C^GGCCG
EclXI 5399 CGGCCG C^GGCCG
Eco52I 5399 CGGCCG C^GGCCG
EcoRV 6757 GATATC GAT^ATC
FseI 6856 GGCCGGCC GGCCGG^CC
HindIII 1906 AAGCTT A^AGCTT
HpaI 4041 GTTAAC GTT^AAC
KasI 3048 GGCGCC G^GCGCC
MamI 2800 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6025 CAATTG C^AATTG
MunI 6025 CAATTG C^AATTG
MvnI 2401 CGCG CG^CG
NarI 3048 GGCGCC GG^CGCC
NruI 2400 TCGCGA TCG^CGA
NspV 8240 TTCGAA TT^CGAA
PmaCI 4204 CACGTG CAC^GTG
PmlI 4204 CACGTG CAC^GTG
Psp1406I 2060 AACGTT AA^CGTT
SfoI 3048 GGCGCC GGC^GCC
SfuI 8240 TTCGAA TT^CGAA
SgrAI 7135 CACCGGCG C[GA]^CCGG[CT]G
SpeI 8350 ACTAGT A^CTAGT
XmnI 578 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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Acc65I 4941 GGTACC G^GTACC
5172 GGTACC
AcsI 6808 GAATTT [GA]^AATT[CT]
6952 AAATTC
AgeI 2730 ACCGGT A^CCGGT
4938 ACCGGT
Aor51HI 2270 AGCGCT AGC^GCT
6938 AGCGCT
ApoI 6808 GAATTT [GA]^AATT[CT]
6952 AAATTC
Asp718I 4941 GGTACC G^GTACC
5172 GGTACC
BciVI 1242 GGATAC GTATCC(6/5)
5512 GTATCC
BsiEI 2728 CGACCG CG[GA][CT]^CG
5399 CGGCCG
BsmBI 2304 GAGACG CGTCTC(1/5)
8531 GAGACG
BspHI 1068 TCATGA T^CATGA
2660 TCATGA
DraIII 3718 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
6178 CACCGAGTG
Eco47III 2270 AGCGCT AGC^GCT
6938 AGCGCT
KpnI 4941 GGTACC GGTAC^C
5172 GGTACC
PinAI 2730 ACCGGT A^CCGGT
4938 ACCGGT
RcaI 1068 TCATGA T^CATGA
2660 TCATGA
ScaI 725 AGTACT AGT^ACT
1655 AGTACT
SexAI 3887 ACCAGGT A^CC[AT]GGT
4560 ACCTGGT
SphI 4630 GCATGC GCATG^C
5970 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI