(Circular) (Six-base) MAPSORT of: pFN21AE9670.seq Check: 4286 from: 1 to: 14395 pFN21AE9670 14395 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5176 5344 10159 10573 5176 Size: 168 4815 414 8998 Fragments arranged by size: 8998 4815 414 168 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 11575 12205 278 Size: 53 83 186 936 10039 630 2468 Fragments arranged by size: 10039 2468 936 630 186 83 53 AccI GT'mk_AC Cuts at: 1937 6911 11738 1937 Size: 4974 4827 4594 Fragments arranged by size: 4974 4827 4594 Acc65I G'GTAC_C Cuts at: 6946 7177 11716 6946 Size: 231 4539 9625 Fragments arranged by size: 9625 4539 231 AclI AA'CG_TT Cuts at: 1563 4066 12522 12895 1563 Size: 2503 8456 373 3063 Fragments arranged by size: 8456 3063 2503 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 3120 3132 3609 4756 6150 6174 7338 Size: 755 733 12 477 1147 1394 24 1164 Cuts at: 7338 7785 8703 9333 9936 9980 10104 11547 12458 Size: 447 918 630 603 44 124 1443 911 Cuts at: 12458 13842 14110 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1443 1394 1384 1164 1147 918 911 755 733 630 603 477 447 268 124 44 24 12 AfeI AGC'GCT Cuts at: 4277 8945 4277 Size: 4668 9727 AflII C'TTAA_G Cuts at: 820 1017 8107 820 Size: 197 7090 7108 Fragments arranged by size: 7108 7090 197 AflIII A'CryG_T Cuts at: 1203 6601 13294 14271 1203 Size: 5398 6693 977 1327 Fragments arranged by size: 6693 5398 1327 977 AgeI A'CCGG_T Cuts at: 4735 6943 13226 4735 Size: 2208 6283 5904 Fragments arranged by size: 6283 5904 2208 AhdI GACnn_n'nnGTC Cuts at: 2743 6567 7909 13124 2743 Size: 3824 1342 5215 4014 Fragments arranged by size: 5215 4014 3824 1342 AleI CACnn'nnGTG Cuts at: 1166 4034 5477 6113 10334 1166 Size: 2868 1443 636 4221 5227 Fragments arranged by size: 5227 4221 2868 1443 636 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3372 8925 10352 1184 Size: 2188 5553 1427 5227 Fragments arranged by size: 5553 5227 2188 1427 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7701 7733 9047 9079 7701 Size: 32 1314 32 13017 Fragments arranged by size: 13017 1314 32 32 AlwNI CAG_nnn'CTG Cuts at: 3380 3500 3776 5093 6857 7045 7198 7240 7366 Size: 120 276 1317 1764 188 153 42 126 Cuts at: 7366 8399 8421 9392 9770 9884 9983 10622 10691 Size: 1033 22 971 378 114 99 639 69 Cuts at: 10691 10721 12138 13710 3380 Size: 30 1417 1572 4065 Fragments arranged by size: 4065 1764 1572 1417 1317 1033 971 639 378 276 188 153 126 120 114 99 69 42 30 22 ApaI G_GGCC'C Cuts at: 2264 2554 3450 3729 5066 5178 6413 7289 7403 Size: 290 896 279 1337 112 1235 876 114 Cuts at: 7403 7744 10428 10752 11434 2264 Size: 341 2684 324 682 5225 Fragments arranged by size: 5225 2684 1337 1235 896 876 682 341 324 290 279 114 112 ApaLI G'TGCA_C Cuts at: 5720 12452 13608 5720 Size: 6732 1156 6507 Fragments arranged by size: 6732 6507 1156 ApoI r'AATT_y Cuts at: 1493 8813 8957 11704 11930 12082 14191 1493 Size: 7320 144 2747 226 152 2109 1697 Fragments arranged by size: 7320 2747 2109 1697 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 12949 160 Size: 872 11917 1606 Fragments arranged by size: 11917 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 14395 AvaI C'yCGr_G Cuts at: 1870 1943 3087 3315 5955 6087 7021 8052 8478 Size: 73 1144 228 2640 132 934 1031 426 Cuts at: 8478 8892 9210 10366 10526 11102 11720 1870 Size: 414 318 1156 160 576 618 4545 Fragments arranged by size: 4545 2640 1156 1144 1031 934 618 576 426 414 318 228 160 132 73 AvrII C'CTAG_G Cuts at: 9326 9326 Size: 14395 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7500 7533 7500 Size: 33 14362 BamHI G'GATC_C Cuts at: 11725 12092 11725 Size: 367 14028 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2383 2517 4071 4902 Size: 325 217 627 414 182 134 1554 831 Cuts at: 4902 5053 5663 5896 6445 6484 6652 6841 6946 Size: 151 610 233 549 39 168 189 105 Cuts at: 6946 7177 7207 7490 7747 7957 9134 9874 10642 Size: 231 30 283 257 210 1177 740 768 Cuts at: 10642 11716 13171 618 Size: 1074 1455 1842 Fragments arranged by size: 1842 1554 1455 1177 1074 831 768 740 627 610 549 414 325 283 257 233 231 217 210 189 182 168 151 134 105 39 30 BanII G_rGCy'C Cuts at: 1391 2264 2394 2554 3123 3450 3729 4108 5066 Size: 873 130 160 569 327 279 379 958 Cuts at: 5066 5100 5178 5357 6011 6094 6413 7028 7289 Size: 34 78 179 654 83 319 615 261 Cuts at: 7289 7379 7403 7744 8046 8059 9000 9211 9966 Size: 90 24 341 302 13 941 211 755 Cuts at: 9966 10428 10752 11434 11714 1391 Size: 462 324 682 280 4072 Fragments arranged by size: 4072 958 941 873 755 682 654 615 569 462 379 341 327 324 319 302 280 279 261 211 179 160 130 90 83 78 34 24 13 BbeI G_GCGC'C Cuts at: 5057 5057 Size: 14395 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3956 4384 6543 9191 928 Size: 971 2057 428 2159 2648 6132 Fragments arranged by size: 6132 2648 2159 2057 971 428 BbvCI CC'TCA_GC Cuts at: 2034 2812 3027 3261 5870 6934 8874 9888 9973 Size: 778 215 234 2609 1064 1940 1014 85 Cuts at: 9973 10092 2034 Size: 119 6337 Fragments arranged by size: 6337 2609 1940 1064 1014 778 234 215 119 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 11350 11384 12586 12620 1903 Size: 34 50 34 9329 34 1202 34 3678 Fragments arranged by size: 9329 3678 1202 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3241 7528 12287 13497 3241 Size: 4287 4759 1210 4139 Fragments arranged by size: 4759 4287 4139 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 14395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3983 5582 5774 Size: 107 122 71 631 605 2311 1599 192 Cuts at: 5774 7812 7874 8780 10261 13006 136 Size: 2038 62 906 1481 2745 1525 Fragments arranged by size: 2745 2311 2038 1599 1525 1481 906 631 605 192 122 107 71 62 BglII A'GATC_T Cuts at: 9754 14390 9754 Size: 4636 9759 BlpI GC'TnA_GC Cuts at: 2337 3302 8802 8898 11812 2337 Size: 965 5500 96 2914 4920 Fragments arranged by size: 5500 4920 2914 965 96 Bme1580I G_kGCm'C Cuts at: 1790 2214 2264 2554 3450 3729 4076 5066 5178 Size: 424 50 290 896 279 347 990 112 Cuts at: 5178 5668 5724 6413 7289 7403 7744 8673 9609 Size: 490 56 689 876 114 341 929 936 Cuts at: 9609 9670 9747 10428 10752 11434 12456 13612 1790 Size: 61 77 681 324 682 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 990 936 929 896 876 689 682 681 490 424 347 341 324 290 279 114 112 77 61 56 50 BmgBI CAC'GTC Cuts at: 1318 8009 1318 Size: 6691 7704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2036 2422 4012 Size: 416 53 83 399 24 619 386 1590 Cuts at: 4012 4057 5066 5117 5336 7232 7379 8089 9380 Size: 45 1009 51 219 1896 147 710 1291 Cuts at: 9380 9392 10679 11429 12126 13084 442 Size: 12 1287 750 697 958 1753 Fragments arranged by size: 1896 1753 1590 1291 1287 1009 958 750 710 697 619 416 399 386 219 147 83 53 51 45 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 14395 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4441 9014 10565 10663 4441 Size: 4573 1551 98 8173 Fragments arranged by size: 8173 4573 1551 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2169 2237 2641 2758 2806 3705 3770 4802 Size: 930 68 404 117 48 899 65 1032 Cuts at: 4802 5070 5352 6687 6711 6792 6900 7146 7170 Size: 268 282 1335 24 81 108 246 24 Cuts at: 7170 7236 7254 7263 7302 7691 8666 8747 8984 Size: 66 18 9 39 389 975 81 237 Cuts at: 8984 8996 9327 9531 9669 9716 9980 10028 10042 Size: 12 331 204 138 47 264 48 14 Cuts at: 10042 10596 13055 1239 Size: 554 2459 2579 Fragments arranged by size: 2579 2459 1335 1032 975 930 899 554 404 389 331 282 268 264 246 237 204 138 117 108 81 81 68 66 65 48 48 47 39 24 24 18 14 12 9 Bpu10I CC'TnA_GC Cuts at: 2034 2460 2812 3027 3261 4793 5010 5094 5351 Size: 426 352 215 234 1532 217 84 257 Cuts at: 5351 5870 6708 6934 8442 8874 9888 9973 10092 Size: 519 838 226 1508 432 1014 85 119 Cuts at: 10092 2034 Size: 6337 Fragments arranged by size: 6337 1532 1508 1014 838 519 432 426 352 257 234 226 217 215 119 85 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2145 2687 4109 6113 7393 8153 9207 9664 12520 Size: 542 1422 2004 1280 760 1054 457 2856 Cuts at: 12520 13385 13683 2145 Size: 865 298 2857 Fragments arranged by size: 2857 2856 2004 1422 1280 1054 865 760 542 457 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 10351 10614 10694 11341 11385 13058 882 Size: 617 8852 263 80 647 44 1673 2219 Fragments arranged by size: 8852 2219 1673 647 617 263 80 44 BsaAI yAC'GTr Cuts at: 493 1204 6211 12186 493 Size: 711 5007 5975 2702 Fragments arranged by size: 5975 5007 2702 711 BsaBI GATnn'nnATC Cuts at: 4809 14389 4809 Size: 9580 4815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3827 4312 5054 7624 Size: 53 83 186 936 2294 485 742 2570 Cuts at: 7624 10168 11572 12202 12584 13219 275 Size: 2544 1404 630 382 635 1451 Fragments arranged by size: 2570 2544 2294 1451 1404 936 742 635 630 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3510 4735 6832 6886 6943 12828 13226 13500 13647 Size: 1225 2097 54 57 5885 398 274 147 Cuts at: 13647 14058 3510 Size: 411 3847 Fragments arranged by size: 5885 3847 2097 1225 411 398 274 147 57 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4043 4073 4302 4315 4332 Size: 30 165 30 2640 30 229 13 17 Cuts at: 4332 4345 4346 4376 5407 5437 7300 7330 8353 Size: 13 1 30 1031 30 1863 30 1023 Cuts at: 8353 8383 9047 9077 9493 9523 9529 9559 1178 Size: 30 664 30 416 30 6 30 6014 Fragments arranged by size: 6014 2640 1863 1031 1023 664 416 229 165 30 30 30 30 30 30 30 30 30 30 17 13 13 6 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 2576 3353 3459 3557 3647 4280 5073 Size: 1354 36 777 106 98 90 633 793 Cuts at: 5073 5365 6192 6253 6804 6807 6816 8537 8702 Size: 292 827 61 551 3 9 1721 165 Cuts at: 8702 8897 8993 9008 9071 9095 9098 9101 9447 Size: 195 96 15 63 24 3 3 346 Cuts at: 9447 9498 9570 9718 9938 10152 11528 11555 11558 Size: 51 72 148 220 214 1376 27 3 Cuts at: 11558 1186 Size: 4023 Fragments arranged by size: 4023 1721 1376 1354 827 793 777 633 551 346 292 220 214 195 165 148 106 98 96 90 72 63 61 51 36 27 24 15 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2116 4076 5106 5356 5843 5989 6352 6412 6457 Size: 1960 1030 250 487 146 363 60 45 Cuts at: 6457 6631 7057 7288 7294 7348 7432 7450 7577 Size: 174 426 231 6 54 84 18 127 Cuts at: 7577 7832 7890 9101 9824 10467 10501 10622 10752 Size: 255 58 1211 723 643 34 121 130 Cuts at: 10752 10767 11060 13598 14094 2116 Size: 15 293 2538 496 2417 Fragments arranged by size: 2538 2417 1960 1211 1030 723 643 496 487 426 363 293 255 250 231 174 146 130 127 121 84 60 58 54 45 34 18 15 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5481 7379 8790 9484 9804 1163 Size: 573 3745 1898 1411 694 320 5754 Fragments arranged by size: 5754 3745 1898 1411 694 573 320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4736 7407 11845 12606 13634 1540 Size: 275 186 2735 2671 4438 761 1028 2301 Fragments arranged by size: 4438 2735 2671 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5100 5724 6011 8046 8193 9000 9966 10546 12456 Size: 624 287 2035 147 807 966 580 1910 Cuts at: 12456 12541 13612 5100 Size: 85 1071 5883 Fragments arranged by size: 5883 2035 1910 1071 966 807 624 580 287 147 85 BsmI GAATG_Cn' Cuts at: 3921 8220 11235 11906 11999 3921 Size: 4299 3015 671 93 6317 Fragments arranged by size: 6317 4299 3015 671 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4303 10530 4303 Size: 6227 8168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2264 2394 2554 3123 3450 3729 Size: 399 424 50 130 160 569 327 279 Cuts at: 3729 4076 4108 5066 5100 5178 5357 5668 5724 Size: 347 32 958 34 78 179 311 56 Cuts at: 5724 6011 6094 6413 7028 7289 7379 7403 7744 Size: 287 83 319 615 261 90 24 341 Cuts at: 7744 8046 8059 8193 8673 9000 9211 9609 9670 Size: 302 13 134 480 327 211 398 61 Cuts at: 9670 9747 9966 10428 10546 10752 11434 11714 12456 Size: 77 219 462 118 206 682 280 742 Cuts at: 12456 12541 13612 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 958 742 682 615 569 480 462 424 399 398 347 341 327 327 319 311 302 287 280 279 261 219 211 206 179 160 134 130 118 90 85 83 78 77 61 56 50 34 32 24 13 BspEI T'CCGG_A Cuts at: 14058 14058 Size: 14395 BspHI T'CATG_A Cuts at: 3073 4665 12284 14014 3073 Size: 1592 7619 1730 3454 Fragments arranged by size: 7619 3454 1730 1592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2703 5176 5344 8271 10159 10573 11750 844 Size: 1859 2473 168 2927 1888 414 1177 3489 Fragments arranged by size: 3489 2927 2473 1888 1859 1177 414 168 BsrBI CCG'CTC Cuts at: 1394 1823 7496 12282 1394 Size: 429 5673 4786 3507 Fragments arranged by size: 5673 4786 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2632 2944 3762 7559 9958 10479 12890 Size: 1183 1384 312 818 3797 2399 521 2411 Cuts at: 12890 13064 65 Size: 174 1396 Fragments arranged by size: 3797 2411 2399 1396 1384 1183 818 521 312 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 6057 6943 8862 9141 11586 13039 Size: 110 2925 1322 886 1919 279 2445 1453 Cuts at: 13039 13226 1700 Size: 187 2869 Fragments arranged by size: 2925 2869 2445 1919 1453 1322 886 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 14395 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 14395 BssSI C'ACGA_G Cuts at: 2522 5726 9183 10839 12455 13467 2522 Size: 3204 3457 1656 1616 1012 3450 Fragments arranged by size: 3457 3450 3204 1656 1616 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6737 7490 8340 9368 794 Size: 5943 753 850 1028 5821 Fragments arranged by size: 5943 5821 1028 850 753 BstBI TT'CG_AA Cuts at: 10246 13199 10246 Size: 2953 11442 BstEII G'GTnAC_C Cuts at: 1184 3892 4738 6569 1184 Size: 2708 846 1831 9010 Fragments arranged by size: 9010 2708 1831 846 BstXI CCAn_nnnn'nTGG Cuts at: 3875 6763 7995 10760 11263 3875 Size: 2888 1232 2765 503 7007 Fragments arranged by size: 7007 2888 2765 1232 503 BstYI r'GATC_y Cuts at: 1972 8276 9754 9777 10717 11725 12092 12477 12494 Size: 6304 1478 23 940 1008 367 385 17 Cuts at: 12494 13946 14032 14044 14390 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 6304 1977 1478 1452 1008 940 385 367 346 86 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2379 3126 3471 5545 9651 10137 11499 11533 2379 Size: 747 345 2074 4106 486 1362 34 5241 Fragments arranged by size: 5241 4106 2074 1362 747 486 345 34 BtgI C'CryG_G Cuts at: 513 1065 2595 3294 3333 3450 4800 5048 5454 Size: 552 1530 699 39 117 1350 248 406 Cuts at: 5454 5790 5907 6624 7512 8248 8433 11256 513 Size: 336 117 717 888 736 185 2823 3652 Fragments arranged by size: 3652 2823 1530 1350 888 736 717 699 552 406 336 248 185 117 117 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4388 6831 487 Size: 683 56 3162 2443 8051 Fragments arranged by size: 8051 3162 2443 683 56 BtsI GCAGTG_nn' Cuts at: 2444 3394 3712 5225 5484 7590 8157 10451 10544 Size: 950 318 1513 259 2106 567 2294 93 Cuts at: 10544 10943 10959 11911 12705 12725 2444 Size: 399 16 952 794 20 4114 Fragments arranged by size: 4114 2294 2106 1513 952 950 794 567 399 318 259 93 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 14395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3968 4003 529 Size: 35 3404 35 10921 Fragments arranged by size: 10921 3404 35 35 DraI TTT'AAA Cuts at: 4084 11699 4084 Size: 7615 6780 DraIII CAC_nnn'GTG Cuts at: 5728 8188 5728 Size: 2460 11935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 13402 809 Size: 507 12086 1802 Fragments arranged by size: 12086 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 3807 6055 Size: 54 1425 50 275 18 768 1209 2248 Cuts at: 6055 6531 6669 7404 8251 8470 8571 8703 8860 Size: 476 138 735 847 219 101 132 157 Cuts at: 8860 9711 10214 11842 12731 14360 8 Size: 851 503 1628 889 1629 43 Fragments arranged by size: 2248 1629 1628 1425 1209 889 851 847 768 735 503 476 275 219 157 138 132 101 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7404 11842 1537 Size: 275 5592 4438 4090 Fragments arranged by size: 5592 4438 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2363 2424 2794 2812 2886 2997 3111 Size: 917 93 61 370 18 74 111 114 Cuts at: 3111 3201 3219 3237 3456 3585 3624 3675 6129 Size: 90 18 18 219 129 39 51 2454 Cuts at: 6129 6202 6244 6601 6834 8546 8679 8688 8751 Size: 73 42 357 233 1712 133 9 63 Cuts at: 8751 9006 10023 11015 11237 12325 1353 Size: 255 1017 992 222 1088 3423 Fragments arranged by size: 3423 2454 1712 1088 1017 992 917 370 357 255 233 222 219 133 129 114 111 93 90 74 73 63 61 51 42 39 18 18 18 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9056 9241 12982 13356 13502 387 Size: 1064 138 7467 185 3741 374 146 1280 Fragments arranged by size: 7467 3741 1280 1064 374 185 146 138 EcoICRI GAG'CTC Cuts at: 5098 6009 8044 8998 9964 5098 Size: 911 2035 954 966 9529 Fragments arranged by size: 9529 2035 966 954 911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2237 2387 2641 2758 2806 3120 Size: 393 537 68 150 254 117 48 314 Cuts at: 3120 3132 3609 3705 3770 4756 4802 5070 5352 Size: 12 477 96 65 986 46 268 282 Cuts at: 5352 6150 6174 6687 6711 6792 6900 7146 7170 Size: 798 24 513 24 81 108 246 24 Cuts at: 7170 7236 7254 7263 7302 7338 7691 7785 8666 Size: 66 18 9 39 36 353 94 881 Cuts at: 8666 8703 8747 8984 8996 9327 9333 9531 9669 Size: 37 44 237 12 331 6 198 138 Cuts at: 9669 9716 9936 9980 10028 10042 10104 10596 11547 Size: 47 220 44 48 14 62 492 951 Cuts at: 11547 12458 13055 13842 14110 1239 Size: 911 597 787 268 1524 Fragments arranged by size: 1524 986 951 911 881 798 787 597 537 513 492 477 393 353 331 314 282 268 268 254 246 237 220 198 150 138 117 108 96 94 81 68 66 65 62 48 48 47 46 44 44 39 37 36 24 24 24 18 14 12 12 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3102 3720 4493 5085 5783 8537 3102 Size: 618 773 592 698 2754 8960 Fragments arranged by size: 8960 2754 773 698 618 592 EcoO109I rG'GnC_Cy Cuts at: 2189 2260 3041 3446 3725 4007 5063 5090 5175 Size: 71 781 405 279 282 1056 27 85 Cuts at: 5175 5278 5289 7130 7740 7741 10020 10142 10232 Size: 103 11 1841 610 1 2279 122 90 Cuts at: 10232 10425 10590 10749 11430 11431 11495 11559 2189 Size: 193 165 159 681 1 64 64 5025 Fragments arranged by size: 5025 2279 1841 1056 781 681 610 405 282 279 193 165 159 122 103 90 85 71 64 64 27 11 1 1 EcoRI G'AATT_C Cuts at: 11704 12082 11704 Size: 378 14017 EcoRV GAT'ATC Cuts at: 8764 8764 Size: 14395 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3344 3344 Size: 14395 FseI GG_CCGG'CC Cuts at: 8866 8866 Size: 14395 FspI TGC'GCA Cuts at: 2504 8090 11630 12901 2504 Size: 5586 3540 1271 3998 Fragments arranged by size: 5586 3998 3540 1271 FspAI rTGC'GCAy Cuts at: 2504 8090 2504 Size: 5586 8809 HaeII r_GCGC'y Cuts at: 1678 2470 4228 4279 5057 5509 7427 8947 11640 Size: 792 1758 51 778 452 1918 1520 2693 Cuts at: 11640 13542 1678 Size: 1902 2531 Fragments arranged by size: 2693 2531 1918 1902 1758 1520 792 778 452 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2311 2343 2729 2761 3593 Size: 32 135 32 739 32 386 32 832 Cuts at: 3593 3625 3952 3984 4302 4315 4334 4346 4347 Size: 32 327 32 318 13 19 12 1 Cuts at: 4347 4378 6553 6585 7228 7260 7300 7332 7895 Size: 31 2175 32 643 32 40 32 563 Cuts at: 7895 7927 8178 8210 8873 8905 10007 10039 10871 Size: 32 251 32 663 32 1102 32 832 Cuts at: 10871 10903 13036 13068 13110 13142 1373 Size: 32 2133 32 42 32 2626 Fragments arranged by size: 2626 2175 2133 1102 832 832 739 663 643 563 386 327 318 251 135 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 19 13 12 1 HincII GTy'rAC Cuts at: 669 1657 1938 4598 4987 6048 6423 11739 11985 Size: 988 281 2660 389 1061 375 5316 246 Cuts at: 11985 669 Size: 3079 Fragments arranged by size: 5316 3079 2660 1061 988 389 375 281 246 HindIII A'AGCT_T Cuts at: 748 3911 748 Size: 3163 11232 HpaI GTT'AAC Cuts at: 6048 11985 6048 Size: 5937 8458 KasI G'GCGC_C Cuts at: 5053 5053 Size: 14395 KpnI G_GTAC'C Cuts at: 6950 7181 11720 6950 Size: 231 4539 9625 Fragments arranged by size: 9625 4539 231 MfeI C'AATT_G Cuts at: 8030 11994 8030 Size: 3964 10431 MluI A'CGCG_T Cuts at: 14271 14271 Size: 14395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 2453 3015 3114 3252 5513 6980 7163 Size: 741 407 562 99 138 2261 1467 183 Cuts at: 7163 7253 7744 10559 10877 11295 13509 13693 1305 Size: 90 491 2815 318 418 2214 184 2007 Fragments arranged by size: 2815 2261 2214 2007 1467 741 562 491 418 407 318 184 183 138 99 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 3809 6533 6671 8253 Size: 54 1425 343 768 1209 2724 138 1582 Cuts at: 8253 8472 8573 8705 9713 10 Size: 219 101 132 1008 4692 Fragments arranged by size: 4692 2724 1582 1425 1209 1008 768 343 219 138 132 101 54 MslI CAynn'nnrTG Cuts at: 518 1166 3078 3192 4034 4685 5477 6113 8204 Size: 648 1912 114 842 651 792 636 2091 Cuts at: 8204 10334 11261 12353 12712 12871 518 Size: 2130 927 1092 359 159 2042 Fragments arranged by size: 2130 2091 2042 1912 1092 927 842 792 651 648 636 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2533 2816 3031 3260 3776 4578 4693 4822 Size: 927 283 215 229 516 802 115 129 Cuts at: 4822 5704 6023 6461 7693 7909 8457 8929 9288 Size: 882 319 438 1232 216 548 472 359 Cuts at: 9288 10091 10315 11811 12488 13636 13881 1606 Size: 803 224 1496 677 1148 245 2120 Fragments arranged by size: 2120 1496 1232 1148 927 882 803 802 677 548 516 472 438 359 319 283 245 229 224 216 215 129 115 NaeI GCC'GGC Cuts at: 6059 8864 11588 6059 Size: 2805 2724 8866 Fragments arranged by size: 8866 2805 2724 NarI GG'CG_CC Cuts at: 5054 5054 Size: 14395 NcoI C'CATG_G Cuts at: 513 1065 2595 3294 3333 5907 7512 8248 8433 Size: 552 1530 699 39 2574 1605 736 185 Cuts at: 8433 11256 513 Size: 2823 3652 Fragments arranged by size: 3652 2823 2574 1605 1530 736 699 552 185 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 14395 NgoMIV G'CCGG_C Cuts at: 6057 8862 11586 6057 Size: 2805 2724 8866 Fragments arranged by size: 8866 2805 2724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 14395 NotI GC'GGCC_GC Cuts at: 11842 11842 Size: 14395 NruI TCG'CGA Cuts at: 4407 4407 Size: 14395 NspI r_CATG'y Cuts at: 1132 1229 4571 6605 6639 7979 8089 10393 11753 Size: 97 3342 2034 34 1340 110 2304 1360 Cuts at: 11753 13298 1132 Size: 1545 2229 Fragments arranged by size: 3342 2304 2229 2034 1545 1360 1340 110 97 34 PasI CC'CwG_GG Cuts at: 1348 5059 5272 6227 7126 7396 10691 10856 11137 Size: 3711 213 955 899 270 3295 165 281 Cuts at: 11137 11491 1348 Size: 354 4252 Fragments arranged by size: 4252 3711 3295 955 899 354 281 270 213 165 PciI A'CATG_T Cuts at: 6601 13294 6601 Size: 6693 7702 PflMI CCAn_nnn'nTGG Cuts at: 1108 3380 5791 7848 9676 10691 11441 1108 Size: 2272 2411 2057 1828 1015 750 4062 Fragments arranged by size: 4062 2411 2272 2057 1828 1015 750 PfoI T'CCnGG_A Cuts at: 3858 6466 6916 7770 8022 8961 10725 3858 Size: 2608 450 854 252 939 1764 7528 Fragments arranged by size: 7528 2608 1764 939 854 450 252 PmeI GTTT'AAAC Cuts at: 11699 11699 Size: 14395 PmlI CAC'GTG Cuts at: 6211 6211 Size: 14395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9047 9079 12464 12496 13977 14009 9047 Size: 32 3385 32 1481 32 9433 Fragments arranged by size: 9433 3385 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4007 5090 5278 5289 7130 10020 10142 11559 4007 Size: 1083 188 11 1841 2890 122 1417 6843 Fragments arranged by size: 6843 2890 1841 1417 1083 188 122 11 PshAI GACnn'nnGTC Cuts at: 1613 4833 7766 8457 9185 1613 Size: 3220 2933 691 728 6823 Fragments arranged by size: 6823 3220 2933 728 691 PsiI TTA'TAA Cuts at: 11965 11965 Size: 14395 PspOMI G'GGCC_C Cuts at: 2260 2550 3446 3725 5062 5174 6409 7285 7399 Size: 290 896 279 1337 112 1235 876 114 Cuts at: 7399 7740 10424 10748 11430 2260 Size: 341 2684 324 682 5225 Fragments arranged by size: 5225 2684 1337 1235 896 876 682 341 324 290 279 114 112 PspXI vC'TCGA_Gb Cuts at: 6087 6087 Size: 14395 PstI C_TGCA'G Cuts at: 830 3772 4194 4583 5001 5232 9293 10876 11747 Size: 2942 422 389 418 231 4061 1583 871 Cuts at: 11747 830 Size: 3478 Fragments arranged by size: 4061 3478 2942 1583 871 422 418 389 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 14395 PvuII CAG'CTG Cuts at: 2816 3031 3260 3776 4693 6023 7909 8929 10091 Size: 215 229 516 917 1330 1886 1020 1162 Cuts at: 10091 2816 Size: 7120 Fragments arranged by size: 7120 1886 1330 1162 1020 917 516 229 215 SacI G_AGCT'C Cuts at: 5100 6011 8046 9000 9966 5100 Size: 911 2035 954 966 9529 Fragments arranged by size: 9529 2035 966 954 911 SalI G'TCGA_C Cuts at: 1936 11737 1936 Size: 9801 4594 SanDI GG'GwC_CC Cuts at: 4007 7130 11559 4007 Size: 3123 4429 6843 Fragments arranged by size: 6843 4429 3123 SapI GCTCTTCn'nnn_ Cuts at: 2270 2794 2812 2997 3111 3456 3675 9006 10023 Size: 524 18 185 114 345 219 5331 1017 Cuts at: 10023 2270 Size: 6642 Fragments arranged by size: 6642 5331 1017 524 345 219 185 114 18 SbfI CC_TGCA'GG Cuts at: 11747 11747 Size: 14395 ScaI AGT'ACT Cuts at: 2732 3662 12643 14355 2732 Size: 930 8981 1712 2772 Fragments arranged by size: 8981 2772 1712 930 SexAI A'CCwGG_T Cuts at: 5892 6565 5892 Size: 673 13722 SfcI C'TryA_G Cuts at: 826 1045 2048 3252 3768 4190 4579 4997 5228 Size: 219 1003 1204 516 422 389 418 231 Cuts at: 5228 6291 9289 10872 11474 11743 12878 13559 13750 Size: 1063 2998 1583 602 269 1135 681 191 Cuts at: 13750 826 Size: 1471 Fragments arranged by size: 2998 1583 1471 1204 1135 1063 1003 681 602 516 422 418 389 269 231 219 191 SfoI GGC'GCC Cuts at: 5055 5055 Size: 14395 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 14395 SgrAI Cr'CCGG_yG Cuts at: 1700 9141 1700 Size: 7441 6954 SmaI CCC'GGG Cuts at: 1872 8054 8480 8894 9212 10368 11722 1872 Size: 6182 426 414 318 1156 1354 4545 Fragments arranged by size: 6182 4545 1354 1156 426 414 318 SmlI C'TyrA_G Cuts at: 820 1017 1943 2160 2666 3315 4124 6087 6128 Size: 197 926 217 506 649 809 1963 41 Cuts at: 6128 7372 8107 8168 9222 9643 11102 12499 13400 Size: 1244 735 61 1054 421 1459 1397 901 Cuts at: 13400 13662 820 Size: 262 1553 Fragments arranged by size: 1963 1553 1459 1397 1244 1054 926 901 809 735 649 506 421 262 217 197 61 41 SnaBI TAC'GTA Cuts at: 493 12186 493 Size: 11693 2702 SpeI A'CTAG_T Cuts at: 152 10355 152 Size: 10203 4192 SphI G_CATG'C Cuts at: 1132 6639 7979 11753 1132 Size: 5507 1340 3774 3774 Fragments arranged by size: 5507 3774 3774 1340 SrfI GCCC'GGGC Cuts at: 9212 9212 Size: 14395 SspI AAT'ATT Cuts at: 5 52 12319 5 Size: 47 12267 2081 Fragments arranged by size: 12267 2081 47 StuI AGG'CCT Cuts at: 1517 4205 4507 6121 8303 8969 9331 9649 1517 Size: 2688 302 1614 2182 666 362 318 6263 Fragments arranged by size: 6263 2688 2182 1614 666 362 318 302 StyI C'CwwG_G Cuts at: 513 1065 2595 2601 3182 3294 3333 3792 5907 Size: 552 1530 6 581 112 39 459 2115 Cuts at: 5907 6480 7419 7512 7536 7994 8248 8433 8784 Size: 573 939 93 24 458 254 185 351 Cuts at: 8784 9051 9326 9636 9834 11143 11256 11608 11834 Size: 267 275 310 198 1309 113 352 226 Cuts at: 11834 513 Size: 3074 Fragments arranged by size: 3074 2115 1530 1309 939 581 573 552 459 458 352 351 310 275 267 254 226 198 185 113 112 93 39 24 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6121 7084 8001 8108 8216 8792 9152 Size: 1255 4541 963 917 107 108 576 360 Cuts at: 9152 9838 10932 11026 12422 12439 12592 12777 13222 Size: 686 1094 94 1396 17 153 185 445 Cuts at: 13222 325 Size: 1498 Fragments arranged by size: 4541 1498 1396 1255 1094 963 917 686 576 445 360 185 153 108 107 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 3660 7879 Size: 274 80 33 51 1443 753 930 4219 Cuts at: 7879 12641 14353 96 Size: 4762 1712 138 Fragments arranged by size: 4762 4219 1712 1443 930 753 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2408 2440 2467 2499 5407 5439 6200 6232 6328 Size: 32 27 32 2908 32 761 32 96 Cuts at: 6328 6357 6360 6389 6438 6470 8353 8385 9493 Size: 29 3 29 49 32 1883 32 1108 Cuts at: 9493 9525 14355 14387 2408 Size: 32 4830 32 2416 Fragments arranged by size: 4830 2908 2416 1883 1108 761 96 49 32 32 32 32 32 32 32 32 29 29 27 3 Tth111I GACn'n_nGTC Cuts at: 1316 7150 7198 7240 10685 1316 Size: 5834 48 42 3445 5026 Fragments arranged by size: 5834 5026 3445 48 42 XbaI T'CTAG_A Cuts at: 11731 11731 Size: 14395 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 5114 6073 6109 8959 9832 10183 2212 Size: 2902 959 36 2850 873 351 6424 Fragments arranged by size: 6424 2902 2850 959 873 351 36 XhoI C'TCGA_G Cuts at: 1943 3315 6087 11102 1943 Size: 1372 2772 5015 5236 Fragments arranged by size: 5236 5015 2772 1372 XmaI C'CCGG_G Cuts at: 1870 8052 8478 8892 9210 10366 11720 1870 Size: 6182 426 414 318 1156 1354 4545 Fragments arranged by size: 6182 4545 1354 1156 426 414 318 XmnI GAAnn'nnTTC Cuts at: 2587 12524 2587 Size: 9937 4458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 11573 12203 276 Size: 53 83 186 936 10039 630 2468 Fragments arranged by size: 10039 2468 936 630 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BfrBI BsiWI BstZ17I NsiI PacI PsrI RsrII SacII SfiI SwaI