(Linear) (Six-base) MAPSORT of: KIEE9670.seq Check: 5148 from: 1 to: 9690 KIEE9670.orf With 182 enzymes: SgfI * December 5, 2014 13:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3171 3339 8154 8568 9690 Size: 3171 168 4815 414 1122 Fragments arranged by size: 4815 3171 1122 414 168 AatII G_ACGT'C Cuts at: 0 9570 9690 Size: 9570 120 AccI GT'mk_AC Cuts at: 0 4906 9690 Size: 4906 4784 Acc65I G'GTAC_C Cuts at: 0 4941 5172 9690 Size: 4941 231 4518 Fragments arranged by size: 4941 4518 231 AclI AA'CG_TT Cuts at: 0 2061 9690 Size: 2061 7629 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 1115 1127 1604 2751 4145 4169 5333 Size: 382 733 12 477 1147 1394 24 1164 Cuts at: 5333 5780 6698 7328 7931 7975 8099 9542 9690 Size: 447 918 630 603 44 124 1443 148 Fragments arranged by size: 1443 1394 1164 1147 918 733 630 603 477 447 382 148 124 44 24 12 AfeI AGC'GCT Cuts at: 0 2272 6940 9690 Size: 2272 4668 2750 Fragments arranged by size: 4668 2750 2272 AflII C'TTAA_G Cuts at: 0 6102 9690 Size: 6102 3588 AflIII A'CryG_T Cuts at: 0 4596 9690 Size: 4596 5094 AgeI A'CCGG_T Cuts at: 0 2730 4938 9690 Size: 2730 2208 4752 Fragments arranged by size: 4752 2730 2208 AhdI GACnn_n'nnGTC Cuts at: 0 738 4562 5904 9690 Size: 738 3824 1342 3786 Fragments arranged by size: 3824 3786 1342 738 AleI CACnn'nnGTG Cuts at: 0 2029 3472 4108 8329 9690 Size: 2029 1443 636 4221 1361 Fragments arranged by size: 4221 2029 1443 1361 636 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1367 6920 8347 9690 Size: 1367 5553 1427 1343 Fragments arranged by size: 5553 1427 1367 1343 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 5696 5728 7042 7074 9690 Size: 5696 32 1314 32 2616 Fragments arranged by size: 5696 2616 1314 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1375 1495 1771 3088 4852 5040 5193 5235 Size: 1375 120 276 1317 1764 188 153 42 Cuts at: 5235 5361 6394 6416 7387 7765 7879 7978 8617 Size: 126 1033 22 971 378 114 99 639 Cuts at: 8617 8686 8716 9690 Size: 69 30 974 Fragments arranged by size: 1764 1375 1317 1033 974 971 639 378 276 188 153 126 120 114 99 69 42 30 22 ApaI G_GGCC'C Cuts at: 0 259 549 1445 1724 3061 3173 4408 5284 Size: 259 290 896 279 1337 112 1235 876 Cuts at: 5284 5398 5739 8423 8747 9429 9690 Size: 114 341 2684 324 682 261 Fragments arranged by size: 2684 1337 1235 896 876 682 341 324 290 279 261 259 114 112 ApaLI G'TGCA_C Cuts at: 0 3715 9690 Size: 3715 5975 ApoI r'AATT_y Cuts at: 0 6808 6952 9690 Size: 6808 144 2738 Fragments arranged by size: 6808 2738 144 AvaI C'yCGr_G Cuts at: 0 1082 1310 3950 4082 5016 6047 6473 6887 Size: 1082 228 2640 132 934 1031 426 414 Cuts at: 6887 7205 8361 8521 9097 9690 Size: 318 1156 160 576 593 Fragments arranged by size: 2640 1156 1082 1031 934 593 576 426 414 318 228 160 132 AvrII C'CTAG_G Cuts at: 0 7321 9690 Size: 7321 2369 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 5495 5528 9690 Size: 5495 33 4162 Fragments arranged by size: 5495 4162 33 BanI G'GyrC_C Cuts at: 0 196 378 512 2066 2897 3048 3658 3891 Size: 196 182 134 1554 831 151 610 233 Cuts at: 3891 4440 4479 4647 4836 4941 5172 5202 5485 Size: 549 39 168 189 105 231 30 283 Cuts at: 5485 5742 5952 7129 7869 8637 9690 Size: 257 210 1177 740 768 1053 Fragments arranged by size: 1554 1177 1053 831 768 740 610 549 283 257 233 231 210 196 189 182 168 151 134 105 39 30 BanII G_rGCy'C Cuts at: 0 259 389 549 1118 1445 1724 2103 3061 Size: 259 130 160 569 327 279 379 958 Cuts at: 3061 3095 3173 3352 4006 4089 4408 5023 5284 Size: 34 78 179 654 83 319 615 261 Cuts at: 5284 5374 5398 5739 6041 6054 6995 7206 7961 Size: 90 24 341 302 13 941 211 755 Cuts at: 7961 8423 8747 9429 9690 Size: 462 324 682 261 Fragments arranged by size: 958 941 755 682 654 615 569 462 379 341 327 324 319 302 279 261 261 259 211 179 160 130 90 83 78 34 24 13 BbeI G_GCGC'C Cuts at: 0 3052 9690 Size: 3052 6638 BbsI GAAGACnn'nnnn_ Cuts at: 0 1951 2379 4538 7186 9690 Size: 1951 428 2159 2648 2504 Fragments arranged by size: 2648 2504 2159 1951 428 BbvCI CC'TCA_GC Cuts at: 0 29 807 1022 1256 3865 4929 6869 7883 Size: 29 778 215 234 2609 1064 1940 1014 Cuts at: 7883 7968 8087 9690 Size: 85 119 1603 Fragments arranged by size: 2609 1940 1603 1064 1014 778 234 215 119 85 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 9345 9379 9690 Size: 9345 34 311 Fragments arranged by size: 9345 311 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1236 5523 9690 Size: 1236 4287 4167 Fragments arranged by size: 4287 4167 1236 BglI GCCn_nnn'nGGC Cuts at: 0 1978 3577 3769 5807 5869 6775 8256 9690 Size: 1978 1599 192 2038 62 906 1481 1434 Fragments arranged by size: 2038 1978 1599 1481 1434 906 192 62 BglII A'GATC_T Cuts at: 0 7749 9690 Size: 7749 1941 BlpI GC'TnA_GC Cuts at: 0 332 1297 6797 6893 9690 Size: 332 965 5500 96 2797 Fragments arranged by size: 5500 2797 965 332 96 Bme1580I G_kGCm'C Cuts at: 0 209 259 549 1445 1724 2071 3061 3173 Size: 209 50 290 896 279 347 990 112 Cuts at: 3173 3663 3719 4408 5284 5398 5739 6668 7604 Size: 490 56 689 876 114 341 929 936 Cuts at: 7604 7665 7742 8423 8747 9429 9690 Size: 61 77 681 324 682 261 Fragments arranged by size: 990 936 929 896 876 689 682 681 490 347 341 324 290 279 261 209 114 112 77 61 56 50 BmgBI CAC'GTC Cuts at: 0 6004 9690 Size: 6004 3686 BmrI ACTGGGnnnn_n' Cuts at: 0 31 417 2007 2052 3061 3112 3331 5227 Size: 31 386 1590 45 1009 51 219 1896 Cuts at: 5227 5374 6084 7375 7387 8674 9424 9690 Size: 147 710 1291 12 1287 750 266 Fragments arranged by size: 1896 1590 1291 1287 1009 750 710 386 266 219 147 51 45 31 12 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2436 7009 8560 8658 9690 Size: 2436 4573 1551 98 1032 Fragments arranged by size: 4573 2436 1551 1032 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 164 232 636 753 801 1700 1765 2797 Size: 164 68 404 117 48 899 65 1032 Cuts at: 2797 3065 3347 4682 4706 4787 4895 5141 5165 Size: 268 282 1335 24 81 108 246 24 Cuts at: 5165 5231 5249 5258 5297 5686 6661 6742 6979 Size: 66 18 9 39 389 975 81 237 Cuts at: 6979 6991 7322 7526 7664 7711 7975 8023 8037 Size: 12 331 204 138 47 264 48 14 Cuts at: 8037 8591 9690 Size: 554 1099 Fragments arranged by size: 1335 1099 1032 975 899 554 404 389 331 282 268 264 246 237 204 164 138 117 108 81 81 68 66 65 48 48 47 39 24 24 18 14 12 9 Bpu10I CC'TnA_GC Cuts at: 0 29 455 807 1022 1256 2788 3005 3089 Size: 29 426 352 215 234 1532 217 84 Cuts at: 3089 3346 3865 4703 4929 6437 6869 7883 7968 Size: 257 519 838 226 1508 432 1014 85 Cuts at: 7968 8087 9690 Size: 119 1603 Fragments arranged by size: 1603 1532 1508 1014 838 519 432 426 352 257 234 226 217 215 119 85 84 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 140 682 2104 4108 5388 6148 7202 7659 Size: 140 542 1422 2004 1280 760 1054 457 Cuts at: 7659 9690 Size: 2031 Fragments arranged by size: 2031 2004 1422 1280 1054 760 542 457 140 BsaI GGTCTCn'nnnn_ Cuts at: 0 8346 8609 8689 9336 9380 9690 Size: 8346 263 80 647 44 310 Fragments arranged by size: 8346 647 310 263 80 44 BsaAI yAC'GTr Cuts at: 0 4206 9690 Size: 4206 5484 BsaBI GATnn'nnATC Cuts at: 0 2804 9690 Size: 2804 6886 BsaHI Gr'CG_yC Cuts at: 0 1822 2307 3049 5619 8163 9567 9690 Size: 1822 485 742 2570 2544 1404 123 Fragments arranged by size: 2570 2544 1822 1404 742 485 123 BsaWI w'CCGG_w Cuts at: 0 1505 2730 4827 4881 4938 9690 Size: 1505 1225 2097 54 57 4752 Fragments arranged by size: 4752 2097 1505 1225 57 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2038 2068 2297 2310 2327 2340 2341 2371 Size: 2038 30 229 13 17 13 1 30 Cuts at: 2371 3402 3432 5295 5325 6348 6378 7042 7072 Size: 1031 30 1863 30 1023 30 664 30 Cuts at: 7072 7488 7518 7524 7554 9690 Size: 416 30 6 30 2136 Fragments arranged by size: 2136 2038 1863 1031 1023 664 416 229 30 30 30 30 30 30 30 30 17 13 13 6 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 535 571 1348 1454 1552 1642 2275 3068 Size: 535 36 777 106 98 90 633 793 Cuts at: 3068 3360 4187 4248 4799 4802 4811 6532 6697 Size: 292 827 61 551 3 9 1721 165 Cuts at: 6697 6892 6988 7003 7066 7090 7093 7096 7442 Size: 195 96 15 63 24 3 3 346 Cuts at: 7442 7493 7565 7713 7933 8147 9523 9550 9553 Size: 51 72 148 220 214 1376 27 3 Cuts at: 9553 9690 Size: 137 Fragments arranged by size: 1721 1376 827 793 777 633 551 535 346 292 220 214 195 165 148 137 106 98 96 90 72 63 61 51 36 27 24 15 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 111 2071 3101 3351 3838 3984 4347 4407 Size: 111 1960 1030 250 487 146 363 60 Cuts at: 4407 4452 4626 5052 5283 5289 5343 5427 5445 Size: 45 174 426 231 6 54 84 18 Cuts at: 5445 5572 5827 5885 7096 7819 8462 8496 8617 Size: 127 255 58 1211 723 643 34 121 Cuts at: 8617 8747 8762 9055 9690 Size: 130 15 293 635 Fragments arranged by size: 1960 1211 1030 723 643 635 487 426 363 293 255 250 231 174 146 130 127 121 111 84 60 58 54 45 34 18 15 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3476 5374 6785 7479 7799 9690 Size: 3476 1898 1411 694 320 1891 Fragments arranged by size: 3476 1898 1891 1411 694 320 BsiEI CG_ry'CG Cuts at: 0 2731 5402 9690 Size: 2731 2671 4288 Fragments arranged by size: 4288 2731 2671 BsiHKAI G_wGCw'C Cuts at: 0 3095 3719 4006 6041 6188 6995 7961 8541 Size: 3095 624 287 2035 147 807 966 580 Cuts at: 8541 9690 Size: 1149 Fragments arranged by size: 3095 2035 1149 966 807 624 580 287 147 BsmI GAATG_Cn' Cuts at: 0 1916 6215 9230 9690 Size: 1916 4299 3015 460 Fragments arranged by size: 4299 3015 1916 460 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2298 8525 9690 Size: 2298 6227 1165 Fragments arranged by size: 6227 2298 1165 Bsp1286I G_dGCh'C Cuts at: 0 209 259 389 549 1118 1445 1724 2071 Size: 209 50 130 160 569 327 279 347 Cuts at: 2071 2103 3061 3095 3173 3352 3663 3719 4006 Size: 32 958 34 78 179 311 56 287 Cuts at: 4006 4089 4408 5023 5284 5374 5398 5739 6041 Size: 83 319 615 261 90 24 341 302 Cuts at: 6041 6054 6188 6668 6995 7206 7604 7665 7742 Size: 13 134 480 327 211 398 61 77 Cuts at: 7742 7961 8423 8541 8747 9429 9690 Size: 219 462 118 206 682 261 Fragments arranged by size: 958 682 615 569 480 462 398 347 341 327 327 319 311 302 287 279 261 261 219 211 209 206 179 160 134 130 118 90 83 78 77 61 56 50 34 32 24 13 BspHI T'CATG_A Cuts at: 0 1068 2660 9690 Size: 1068 1592 7030 Fragments arranged by size: 7030 1592 1068 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 698 3171 3339 6266 8154 8568 9690 Size: 698 2473 168 2927 1888 414 1122 Fragments arranged by size: 2927 2473 1888 1122 698 414 168 BsrBI CCG'CTC Cuts at: 0 5491 9690 Size: 5491 4199 BsrDI GCAATG_nn' Cuts at: 0 627 939 1757 5554 7953 8474 9690 Size: 627 312 818 3797 2399 521 1216 Fragments arranged by size: 3797 2399 1216 818 627 521 312 BsrFI r'CCGG_y Cuts at: 0 2730 4052 4938 6857 7136 9581 9690 Size: 2730 1322 886 1919 279 2445 109 Fragments arranged by size: 2730 2445 1919 1322 886 279 109 BssSI C'ACGA_G Cuts at: 0 517 3721 7178 8834 9690 Size: 517 3204 3457 1656 856 Fragments arranged by size: 3457 3204 1656 856 517 BstAPI GCAn_nnn'nTGC Cuts at: 0 4732 5485 6335 7363 9690 Size: 4732 753 850 1028 2327 Fragments arranged by size: 4732 2327 1028 850 753 BstBI TT'CG_AA Cuts at: 0 8241 9690 Size: 8241 1449 BstEII G'GTnAC_C Cuts at: 0 1887 2733 4564 9690 Size: 1887 846 1831 5126 Fragments arranged by size: 5126 1887 1831 846 BstXI CCAn_nnnn'nTGG Cuts at: 0 1870 4758 5990 8755 9258 9690 Size: 1870 2888 1232 2765 503 432 Fragments arranged by size: 2888 2765 1870 1232 503 432 BstYI r'GATC_y Cuts at: 0 6271 7749 7772 8712 9690 Size: 6271 1478 23 940 978 Fragments arranged by size: 6271 1478 978 940 23 Bsu36I CC'TnA_GG Cuts at: 0 374 1121 1466 3540 7646 8132 9494 9528 Size: 374 747 345 2074 4106 486 1362 34 Cuts at: 9528 9690 Size: 162 Fragments arranged by size: 4106 2074 1362 747 486 374 345 162 34 BtgI C'CryG_G Cuts at: 0 590 1289 1328 1445 2795 3043 3449 3785 Size: 590 699 39 117 1350 248 406 336 Cuts at: 3785 3902 4619 5507 6243 6428 9251 9690 Size: 117 717 888 736 185 2823 439 Fragments arranged by size: 2823 1350 888 736 717 699 590 439 406 336 248 185 117 117 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2383 4826 9690 Size: 2383 2443 4864 Fragments arranged by size: 4864 2443 2383 BtsI GCAGTG_nn' Cuts at: 0 439 1389 1707 3220 3479 5585 6152 8446 Size: 439 950 318 1513 259 2106 567 2294 Cuts at: 8446 8539 8938 8954 9690 Size: 93 399 16 736 Fragments arranged by size: 2294 2106 1513 950 736 567 439 399 318 259 93 16 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1963 1998 9690 Size: 1963 35 7692 Fragments arranged by size: 7692 1963 35 DraI TTT'AAA Cuts at: 0 2079 9690 Size: 2079 7611 DraIII CAC_nnn'GTG Cuts at: 0 3723 6183 9690 Size: 3723 2460 3507 Fragments arranged by size: 3723 3507 2460 EaeI y'GGCC_r Cuts at: 0 593 1802 4050 4526 4664 5399 6246 6465 Size: 593 1209 2248 476 138 735 847 219 Cuts at: 6465 6566 6698 6855 7706 8209 9690 Size: 101 132 157 851 503 1481 Fragments arranged by size: 2248 1481 1209 851 847 735 593 503 476 219 157 138 132 101 EagI C'GGCC_G Cuts at: 0 5399 9690 Size: 5399 4291 EarI CTCTTCn'nnn_ Cuts at: 0 265 358 419 789 807 881 992 1106 Size: 265 93 61 370 18 74 111 114 Cuts at: 1106 1196 1214 1232 1451 1580 1619 1670 4124 Size: 90 18 18 219 129 39 51 2454 Cuts at: 4124 4197 4239 4596 4829 6541 6674 6683 6746 Size: 73 42 357 233 1712 133 9 63 Cuts at: 6746 7001 8018 9010 9232 9690 Size: 255 1017 992 222 458 Fragments arranged by size: 2454 1712 1017 992 458 370 357 265 255 233 222 219 133 129 114 111 93 90 74 73 63 61 51 42 39 18 18 18 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 7051 7236 9690 Size: 7051 185 2454 Fragments arranged by size: 7051 2454 185 EcoICRI GAG'CTC Cuts at: 0 3093 4004 6039 6993 7959 9690 Size: 3093 911 2035 954 966 1731 Fragments arranged by size: 3093 2035 1731 966 954 911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 164 232 382 636 753 801 1115 1127 Size: 164 68 150 254 117 48 314 12 Cuts at: 1127 1604 1700 1765 2751 2797 3065 3347 4145 Size: 477 96 65 986 46 268 282 798 Cuts at: 4145 4169 4682 4706 4787 4895 5141 5165 5231 Size: 24 513 24 81 108 246 24 66 Cuts at: 5231 5249 5258 5297 5333 5686 5780 6661 6698 Size: 18 9 39 36 353 94 881 37 Cuts at: 6698 6742 6979 6991 7322 7328 7526 7664 7711 Size: 44 237 12 331 6 198 138 47 Cuts at: 7711 7931 7975 8023 8037 8099 8591 9542 9690 Size: 220 44 48 14 62 492 951 148 Fragments arranged by size: 986 951 881 798 513 492 477 353 331 314 282 268 254 246 237 220 198 164 150 148 138 117 108 96 94 81 68 66 65 62 48 48 47 46 44 44 39 37 36 24 24 24 18 14 12 12 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1097 1715 2488 3080 3778 6532 9690 Size: 1097 618 773 592 698 2754 3158 Fragments arranged by size: 3158 2754 1097 773 698 618 592 EcoO109I rG'GnC_Cy Cuts at: 0 184 255 1036 1441 1720 2002 3058 3085 Size: 184 71 781 405 279 282 1056 27 Cuts at: 3085 3170 3273 3284 5125 5735 5736 8015 8137 Size: 85 103 11 1841 610 1 2279 122 Cuts at: 8137 8227 8420 8585 8744 9425 9426 9490 9554 Size: 90 193 165 159 681 1 64 64 Cuts at: 9554 9690 Size: 136 Fragments arranged by size: 2279 1841 1056 781 681 610 405 282 279 193 184 165 159 136 122 103 90 85 71 64 64 27 11 1 1 EcoRV GAT'ATC Cuts at: 0 6759 9690 Size: 6759 2931 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1339 9690 Size: 1339 8351 FseI GG_CCGG'CC Cuts at: 0 6861 9690 Size: 6861 2829 FspI TGC'GCA Cuts at: 0 499 6085 9625 9690 Size: 499 5586 3540 65 Fragments arranged by size: 5586 3540 499 65 FspAI rTGC'GCAy Cuts at: 0 499 6085 9690 Size: 499 5586 3605 Fragments arranged by size: 5586 3605 499 HaeII r_GCGC'y Cuts at: 0 465 2223 2274 3052 3504 5422 6942 9635 Size: 465 1758 51 778 452 1918 1520 2693 Cuts at: 9635 9690 Size: 55 Fragments arranged by size: 2693 1918 1758 1520 778 465 452 55 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 306 338 724 756 1588 1620 1947 1979 Size: 306 32 386 32 832 32 327 32 Cuts at: 1979 2297 2310 2329 2341 2342 2373 4548 4580 Size: 318 13 19 12 1 31 2175 32 Cuts at: 4580 5223 5255 5295 5327 5890 5922 6173 6205 Size: 643 32 40 32 563 32 251 32 Cuts at: 6205 6868 6900 8002 8034 8866 8898 9690 Size: 663 32 1102 32 832 32 792 Fragments arranged by size: 2175 1102 832 832 792 663 643 563 386 327 318 306 251 40 32 32 32 32 32 32 32 32 32 32 32 32 31 19 13 12 1 HincII GTy'rAC Cuts at: 0 2593 2982 4043 4418 9690 Size: 2593 389 1061 375 5272 Fragments arranged by size: 5272 2593 1061 389 375 HindIII A'AGCT_T Cuts at: 0 1906 9690 Size: 1906 7784 HpaI GTT'AAC Cuts at: 0 4043 9690 Size: 4043 5647 KasI G'GCGC_C Cuts at: 0 3048 9690 Size: 3048 6642 KpnI G_GTAC'C Cuts at: 0 4945 5176 9690 Size: 4945 231 4514 Fragments arranged by size: 4945 4514 231 MfeI C'AATT_G Cuts at: 0 6025 9690 Size: 6025 3665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 41 448 1010 1109 1247 3508 4975 5158 Size: 41 407 562 99 138 2261 1467 183 Cuts at: 5158 5248 5739 8554 8872 9290 9690 Size: 90 491 2815 318 418 400 Fragments arranged by size: 2815 2261 1467 562 491 418 407 400 318 183 138 99 90 41 MscI TGG'CCA Cuts at: 0 595 1804 4528 4666 6248 6467 6568 6700 Size: 595 1209 2724 138 1582 219 101 132 Cuts at: 6700 7708 9690 Size: 1008 1982 Fragments arranged by size: 2724 1982 1582 1209 1008 595 219 138 132 101 MslI CAynn'nnrTG Cuts at: 0 1073 1187 2029 2680 3472 4108 6199 8329 Size: 1073 114 842 651 792 636 2091 2130 Cuts at: 8329 9256 9690 Size: 927 434 Fragments arranged by size: 2130 2091 1073 927 842 792 651 636 434 114 MspA1I CmG'CkG Cuts at: 0 528 811 1026 1255 1771 2573 2688 2817 Size: 528 283 215 229 516 802 115 129 Cuts at: 2817 3699 4018 4456 5688 5904 6452 6924 7283 Size: 882 319 438 1232 216 548 472 359 Cuts at: 7283 8086 8310 9690 Size: 803 224 1380 Fragments arranged by size: 1380 1232 882 803 802 548 528 516 472 438 359 319 283 229 224 216 215 129 115 NaeI GCC'GGC Cuts at: 0 4054 6859 9583 9690 Size: 4054 2805 2724 107 Fragments arranged by size: 4054 2805 2724 107 NarI GG'CG_CC Cuts at: 0 3049 9690 Size: 3049 6641 NcoI C'CATG_G Cuts at: 0 590 1289 1328 3902 5507 6243 6428 9251 Size: 590 699 39 2574 1605 736 185 2823 Cuts at: 9251 9690 Size: 439 Fragments arranged by size: 2823 2574 1605 736 699 590 439 185 39 NgoMIV G'CCGG_C Cuts at: 0 4052 6857 9581 9690 Size: 4052 2805 2724 109 Fragments arranged by size: 4052 2805 2724 109 NruI TCG'CGA Cuts at: 0 2402 9690 Size: 2402 7288 NspI r_CATG'y Cuts at: 0 2566 4600 4634 5974 6084 8388 9690 Size: 2566 2034 34 1340 110 2304 1302 Fragments arranged by size: 2566 2304 2034 1340 1302 110 34 PasI CC'CwG_GG Cuts at: 0 3054 3267 4222 5121 5391 8686 8851 9132 Size: 3054 213 955 899 270 3295 165 281 Cuts at: 9132 9486 9690 Size: 354 204 Fragments arranged by size: 3295 3054 955 899 354 281 270 213 204 165 PciI A'CATG_T Cuts at: 0 4596 9690 Size: 4596 5094 PflMI CCAn_nnn'nTGG Cuts at: 0 1375 3786 5843 7671 8686 9436 9690 Size: 1375 2411 2057 1828 1015 750 254 Fragments arranged by size: 2411 2057 1828 1375 1015 750 254 PfoI T'CCnGG_A Cuts at: 0 1853 4461 4911 5765 6017 6956 8720 9690 Size: 1853 2608 450 854 252 939 1764 970 Fragments arranged by size: 2608 1853 1764 970 939 854 450 252 PmlI CAC'GTG Cuts at: 0 4206 9690 Size: 4206 5484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 7042 7074 9690 Size: 7042 32 2616 Fragments arranged by size: 7042 2616 32 PpuMI rG'GwC_Cy Cuts at: 0 2002 3085 3273 3284 5125 8015 8137 9554 Size: 2002 1083 188 11 1841 2890 122 1417 Cuts at: 9554 9690 Size: 136 Fragments arranged by size: 2890 2002 1841 1417 1083 188 136 122 11 PshAI GACnn'nnGTC Cuts at: 0 2828 5761 6452 7180 9690 Size: 2828 2933 691 728 2510 Fragments arranged by size: 2933 2828 2510 728 691 PspOMI G'GGCC_C Cuts at: 0 255 545 1441 1720 3057 3169 4404 5280 Size: 255 290 896 279 1337 112 1235 876 Cuts at: 5280 5394 5735 8419 8743 9425 9690 Size: 114 341 2684 324 682 265 Fragments arranged by size: 2684 1337 1235 896 876 682 341 324 290 279 265 255 114 112 PspXI vC'TCGA_Gb Cuts at: 0 4082 9690 Size: 4082 5608 PstI C_TGCA'G Cuts at: 0 1767 2189 2578 2996 3227 7288 8871 9690 Size: 1767 422 389 418 231 4061 1583 819 Fragments arranged by size: 4061 1767 1583 819 422 418 389 231 PvuII CAG'CTG Cuts at: 0 811 1026 1255 1771 2688 4018 5904 6924 Size: 811 215 229 516 917 1330 1886 1020 Cuts at: 6924 8086 9690 Size: 1162 1604 Fragments arranged by size: 1886 1604 1330 1162 1020 917 811 516 229 215 SacI G_AGCT'C Cuts at: 0 3095 4006 6041 6995 7961 9690 Size: 3095 911 2035 954 966 1729 Fragments arranged by size: 3095 2035 1729 966 954 911 SanDI GG'GwC_CC Cuts at: 0 2002 5125 9554 9690 Size: 2002 3123 4429 136 Fragments arranged by size: 4429 3123 2002 136 SapI GCTCTTCn'nnn_ Cuts at: 0 265 789 807 992 1106 1451 1670 7001 Size: 265 524 18 185 114 345 219 5331 Cuts at: 7001 8018 9690 Size: 1017 1672 Fragments arranged by size: 5331 1672 1017 524 345 265 219 185 114 18 ScaI AGT'ACT Cuts at: 0 727 1657 9690 Size: 727 930 8033 Fragments arranged by size: 8033 930 727 SexAI A'CCwGG_T Cuts at: 0 3887 4560 9690 Size: 3887 673 5130 Fragments arranged by size: 5130 3887 673 SfcI C'TryA_G Cuts at: 0 43 1247 1763 2185 2574 2992 3223 4286 Size: 43 1204 516 422 389 418 231 1063 Cuts at: 4286 7284 8867 9469 9690 Size: 2998 1583 602 221 Fragments arranged by size: 2998 1583 1204 1063 602 516 422 418 389 231 221 43 SfoI GGC'GCC Cuts at: 0 3050 9690 Size: 3050 6640 SgrAI Cr'CCGG_yG Cuts at: 0 7136 9690 Size: 7136 2554 SmaI CCC'GGG Cuts at: 0 6049 6475 6889 7207 8363 9690 Size: 6049 426 414 318 1156 1327 Fragments arranged by size: 6049 1327 1156 426 414 318 SmlI C'TyrA_G Cuts at: 0 155 661 1310 2119 4082 4123 5367 6102 Size: 155 506 649 809 1963 41 1244 735 Cuts at: 6102 6163 7217 7638 9097 9690 Size: 61 1054 421 1459 593 Fragments arranged by size: 1963 1459 1244 1054 809 735 649 593 506 421 155 61 41 SpeI A'CTAG_T Cuts at: 0 8350 9690 Size: 8350 1340 SphI G_CATG'C Cuts at: 0 4634 5974 9690 Size: 4634 1340 3716 Fragments arranged by size: 4634 3716 1340 SrfI GCCC'GGGC Cuts at: 0 7207 9690 Size: 7207 2483 StuI AGG'CCT Cuts at: 0 2200 2502 4116 6298 6964 7326 7644 9690 Size: 2200 302 1614 2182 666 362 318 2046 Fragments arranged by size: 2200 2182 2046 1614 666 362 318 302 StyI C'CwwG_G Cuts at: 0 590 596 1177 1289 1328 1787 3902 4475 Size: 590 6 581 112 39 459 2115 573 Cuts at: 4475 5414 5507 5531 5989 6243 6428 6779 7046 Size: 939 93 24 458 254 185 351 267 Cuts at: 7046 7321 7631 7829 9138 9251 9603 9690 Size: 275 310 198 1309 113 352 87 Fragments arranged by size: 2115 1309 939 590 581 573 459 458 352 351 310 275 267 254 198 185 113 112 93 87 39 24 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 4116 5079 5996 6103 6211 6787 7147 7833 Size: 4116 963 917 107 108 576 360 686 Cuts at: 7833 8927 9021 9690 Size: 1094 94 669 Fragments arranged by size: 4116 1094 963 917 686 669 576 360 108 107 94 TatI w'GTAC_w Cuts at: 0 725 1655 5874 9690 Size: 725 930 4219 3816 Fragments arranged by size: 4219 3816 930 725 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 403 435 462 494 3402 3434 4195 4227 Size: 403 32 27 32 2908 32 761 32 Cuts at: 4227 4323 4352 4355 4384 4433 4465 6348 6380 Size: 96 29 3 29 49 32 1883 32 Cuts at: 6380 7488 7520 9690 Size: 1108 32 2170 Fragments arranged by size: 2908 2170 1883 1108 761 403 96 49 32 32 32 32 32 32 32 29 29 27 3 Tth111I GACn'n_nGTC Cuts at: 0 5145 5193 5235 8680 9690 Size: 5145 48 42 3445 1010 Fragments arranged by size: 5145 3445 1010 48 42 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 207 3109 4068 4104 6954 7827 8178 9690 Size: 207 2902 959 36 2850 873 351 1512 Fragments arranged by size: 2902 2850 1512 959 873 351 207 36 XhoI C'TCGA_G Cuts at: 0 1310 4082 9097 9690 Size: 1310 2772 5015 593 Fragments arranged by size: 5015 2772 1310 593 XmaI C'CCGG_G Cuts at: 0 6047 6473 6887 7205 8361 9690 Size: 6047 426 414 318 1156 1329 Fragments arranged by size: 6047 1329 1156 426 414 318 XmnI GAAnn'nnTTC Cuts at: 0 582 9690 Size: 582 9108 ZraI GAC'GTC Cuts at: 0 9568 9690 Size: 9568 122 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AseI AsiSI BamHI BclI BfrBI BmtI BsiWI BspEI BsrGI BssHII BstZ17I ClaI DrdI EcoRI MluI NdeI NheI NotI NsiI PacI PmeI PsiI PsrI PvuI RsrII SacII SalI SbfI SfiI SgfI SnaBI SspI SwaI XbaI