(Circular) (Six-base) MAPSORT of: pFN21AE5535.seq Check: 8303 from: 1 to: 6070 pFN21AE5535 6070 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 2195 3391 2195 Size: 1196 4874 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2035 2732 3245 4133 5517 5785 1632 Size: 403 697 513 888 1384 268 1917 Fragments arranged by size: 1917 1384 888 697 513 403 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 2182 4969 5946 1203 Size: 979 2787 977 1327 Fragments arranged by size: 2787 1327 979 977 AgeI A'CCGG_T Cuts at: 3311 4901 3311 Size: 1590 4480 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 2834 2918 1166 Size: 1668 84 4318 Fragments arranged by size: 4318 1668 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2592 2624 3212 3244 2592 Size: 32 588 32 5418 Fragments arranged by size: 5418 588 32 32 AlwNI CAG_nnn'CTG Cuts at: 2092 2435 3813 5385 2092 Size: 343 1378 1572 2777 Fragments arranged by size: 2777 1572 1378 343 ApaI G_GGCC'C Cuts at: 2986 2986 Size: 6070 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 3379 3605 3757 5866 1493 Size: 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2322 2395 2496 2766 2978 3212 3395 Size: 73 379 73 101 270 212 234 183 Cuts at: 3395 1870 Size: 4545 Fragments arranged by size: 4545 379 270 234 212 183 101 73 73 AvrII C'CTAG_G Cuts at: 2890 2890 Size: 6070 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 2828 3308 3391 4846 Size: 325 217 627 408 633 480 83 1455 Cuts at: 4846 618 Size: 1842 Fragments arranged by size: 1842 1455 633 627 480 408 325 217 83 BanII G_rGCy'C Cuts at: 1391 2323 2889 2986 3389 1391 Size: 932 566 97 403 4072 Fragments arranged by size: 4072 932 566 403 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 2228 928 Size: 971 266 63 4770 Fragments arranged by size: 4770 971 266 63 BbvCI CC'TCA_GC Cuts at: 2259 3089 2259 Size: 830 5240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 2424 1548 Size: 876 5194 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 4681 136 Size: 107 122 71 631 605 478 2531 1525 Fragments arranged by size: 2531 1525 631 605 478 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 2683 2763 2986 4131 5287 1790 Size: 893 80 223 1145 1156 2573 Fragments arranged by size: 2573 1156 1145 893 223 80 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2648 2750 3801 Size: 416 53 83 399 24 1231 102 1051 Cuts at: 3801 4759 442 Size: 958 1753 Fragments arranged by size: 1753 1231 1051 958 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2993 2993 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 2795 2972 4730 1239 Size: 1385 171 177 1758 2579 Fragments arranged by size: 2579 1758 1385 177 171 Bpu10I CC'TnA_GC Cuts at: 2259 3089 2259 Size: 830 5240 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2705 4195 5060 5358 2705 Size: 1490 865 298 3417 Fragments arranged by size: 3417 1490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2063 2642 4733 882 Size: 617 564 579 2091 2219 Fragments arranged by size: 2219 2091 617 579 564 BsaAI yAC'GTr Cuts at: 493 1204 2167 3861 493 Size: 711 963 1694 2702 Fragments arranged by size: 2702 1694 963 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 3877 4259 4894 Size: 53 83 186 936 509 1835 382 635 Cuts at: 4894 275 Size: 1451 Fragments arranged by size: 1835 1451 936 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3297 3311 4503 4901 5175 5322 5733 3297 Size: 14 1192 398 274 147 411 3634 Fragments arranged by size: 3634 1192 411 398 274 147 14 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2792 2822 1178 Size: 30 165 30 1389 30 4426 Fragments arranged by size: 4426 1389 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2327 2660 3359 3362 1186 Size: 1120 21 333 699 3 3894 Fragments arranged by size: 3894 1120 699 333 21 3 BseYI C'CCAG_C Cuts at: 5273 5769 5273 Size: 496 5574 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2421 2741 1163 Size: 573 685 320 4492 Fragments arranged by size: 4492 685 573 320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2071 3520 4281 5309 1540 Size: 275 186 21 49 1449 761 1028 2301 Fragments arranged by size: 2301 1449 1028 761 275 186 49 21 BsiHKAI G_wGCw'C Cuts at: 2323 2505 2889 4131 4216 5287 2323 Size: 182 384 1242 85 1071 3106 Fragments arranged by size: 3106 1242 1071 384 182 85 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 BsmBI CGTCTCn'nnnn_ Cuts at: 2868 2868 Size: 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2323 2505 2683 2763 2889 2986 3389 Size: 399 533 182 178 80 126 97 403 Cuts at: 3389 4131 4216 5287 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 533 403 399 182 178 126 97 85 80 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3425 844 Size: 2581 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2117 4565 4739 65 Size: 1183 869 2448 174 1396 Fragments arranged by size: 2448 1396 1183 869 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 3311 4714 4901 1700 Size: 110 256 1245 1403 187 2869 Fragments arranged by size: 2869 1403 1245 256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 2035 1925 Size: 110 5960 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstXI CCAn_nnnn'nTGG Cuts at: 3331 3331 Size: 6070 BstYI r'GATC_y Cuts at: 1972 2607 3400 3767 4152 4169 5621 5707 5719 Size: 635 793 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 793 635 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2134 2145 2215 2824 2832 513 Size: 552 1069 11 70 609 8 3751 Fragments arranged by size: 3751 1069 609 552 70 11 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 2361 2433 3586 4380 4400 2361 Size: 72 1153 794 20 4031 Fragments arranged by size: 4031 1153 794 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 2865 3374 2865 Size: 509 5561 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2068 2142 Size: 54 1425 50 275 18 189 49 74 Cuts at: 2142 2203 2514 2821 3106 3333 3517 4406 6035 Size: 61 311 307 285 227 184 889 1629 Cuts at: 6035 8 Size: 43 Fragments arranged by size: 1629 1425 889 311 307 285 275 227 189 184 74 61 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 2068 3517 1537 Size: 275 207 49 1449 4090 Fragments arranged by size: 4090 1449 275 207 49 EarI CTCTTCn'nnn_ Cuts at: 1353 4000 1353 Size: 2647 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2321 2887 2321 Size: 566 5504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2624 2732 2795 2972 3245 4133 Size: 393 403 589 108 63 177 273 888 Cuts at: 4133 4730 5517 5785 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 589 403 393 273 268 177 108 63 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2703 3092 2084 Size: 619 389 5062 Fragments arranged by size: 5062 619 389 EcoO109I rG'GnC_Cy Cuts at: 2695 2770 2982 2983 3022 3220 2695 Size: 75 212 1 39 198 5545 Fragments arranged by size: 5545 212 198 75 39 1 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 5217 1678 Size: 3539 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4711 4743 4785 4817 1373 Size: 32 135 32 3139 32 42 32 2626 Fragments arranged by size: 3139 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 2199 3395 2199 Size: 1196 4874 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3155 5184 5368 1305 Size: 1850 2029 184 2007 Fragments arranged by size: 2029 2007 1850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 3335 10 Size: 54 1425 343 684 819 2745 Fragments arranged by size: 2745 1425 819 684 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 2459 2756 2834 2918 2933 4028 Size: 648 1021 272 297 78 84 15 1095 Cuts at: 4028 4387 4546 518 Size: 359 159 2042 Fragments arranged by size: 2042 1095 1021 648 359 297 272 159 84 78 15 MspA1I CmG'CkG Cuts at: 1606 2055 2136 3486 4163 5311 5556 1606 Size: 449 81 1350 677 1148 245 2120 Fragments arranged by size: 2120 1350 1148 677 449 245 81 NaeI GCC'GGC Cuts at: 2068 2068 Size: 6070 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5518 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NgoMIV G'CCGG_C Cuts at: 2066 2066 Size: 6070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 2019 3517 2019 Size: 1498 4572 NspI r_CATG'y Cuts at: 1132 1229 2186 2540 3428 4973 1132 Size: 97 957 354 888 1545 2229 Fragments arranged by size: 2229 1545 957 888 354 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 2182 4969 2182 Size: 2787 3283 PflMI CCAn_nnn'nTGG Cuts at: 1108 3067 1108 Size: 1959 4111 PfoI T'CCnGG_A Cuts at: 2571 2610 2571 Size: 39 6031 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2695 2770 3220 2695 Size: 75 450 5545 Fragments arranged by size: 5545 450 75 PshAI GACnn'nnGTC Cuts at: 1613 2654 1613 Size: 1041 5029 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PspOMI G'GGCC_C Cuts at: 2982 2982 Size: 6070 PstI C_TGCA'G Cuts at: 830 2088 2239 3422 830 Size: 1258 151 1183 3478 Fragments arranged by size: 3478 1258 1183 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 SacI G_AGCT'C Cuts at: 2323 2889 2323 Size: 566 5504 SacII CC_GC'GG Cuts at: 2137 2137 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SanDI GG'GwC_CC Cuts at: 2695 2695 Size: 6070 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SfcI C'TryA_G Cuts at: 826 1045 2084 2235 3418 4553 5234 5425 826 Size: 219 1039 151 1183 1135 681 191 1471 Fragments arranged by size: 1471 1183 1135 1039 681 219 191 151 SfiI GGCCn_nnn'nGGCC Cuts at: 2150 2150 Size: 6070 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2397 2768 3214 3397 1872 Size: 525 371 446 183 4545 Fragments arranged by size: 4545 525 446 371 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2684 4174 5075 5337 820 Size: 197 926 741 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 741 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 2709 1517 Size: 1192 4878 StyI C'CwwG_G Cuts at: 513 1065 2890 3509 513 Size: 552 1825 619 3074 Fragments arranged by size: 3074 1825 619 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4097 4114 4267 4452 4897 325 Size: 1255 2517 17 153 185 445 1498 Fragments arranged by size: 2517 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 4316 6028 Size: 274 80 33 51 1443 831 1508 1712 Cuts at: 6028 96 Size: 138 Fragments arranged by size: 1712 1508 1443 831 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 2567 1316 Size: 1251 4819 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2206 2011 Size: 195 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 2395 2766 3212 3395 1870 Size: 525 371 446 183 4545 Fragments arranged by size: 4545 525 446 371 183 XmnI GAAnn'nnTTC Cuts at: 2783 4199 2783 Size: 1416 4654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SapI SexAI SfoI SrfI SwaI