################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 25 GGCGTC G[GA]^CG[CT]C AluI 607 AGCT AG^CT Aor51HI 101 AGCGCT AGC^GCT BalI 647 TGGCCA TGG^CCA BclI 399 TGATCA T^GATCA BfaI 82 CTAG C^TAG BglII 566 AGATCT A^GATCT BsaHI 25 GGCGTC G[GA]^CG[CT]C BsaI 240 GAGACC GGTCTC(1/5) BsgI 502 CTGCAC GTGCAG(16/14) BsiEI 507 CGGCCG CG[GA][CT]^CG BsmBI 544 GAGACG CGTCTC(1/5) BsmI 666 GAATGC GAATGC(1/-1) BspHI 29 TCATGA T^CATGA BspMI 441 GCAGGT ACCTGC(4/8) BsrDI 137 GCAATG GCAATG(2/0) BssSI 709 CACGAG CACGAG(-5/-1) BstAPI 441 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DsaI 72 CCATGG C^C[GA][CT]GG EagI 507 CGGCCG C^GGCCG EclXI 507 CGGCCG C^GGCCG Eco47III 101 AGCGCT AGC^GCT Eco52I 507 CGGCCG C^GGCCG EcoNI 591 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 72 CCATGG C^C[AT][AT]GG FbaI 399 TGATCA T^GATCA Hin1I 25 GGCGTC G[GA]^CG[CT]C MaeI 82 CTAG C^TAG MluNI 647 TGGCCA TGG^CCA MscI 647 TGGCCA TGG^CCA NcoI 72 CCATGG C^CATGG NlaIV 704 GGCCCC GG[ACGT]^[ACGT]CC PshAI 33 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 448 CTGCAG CTGCA^G RcaI 29 TCATGA T^CATGA ScaI 653 AGTACT AGT^ACT SexAI 392 ACCTGGT A^CC[AT]GGT SfaNI 630 GATGC GCATC(5/9) SfcI 448 CTGCAG C^T[GA][CT]AG SgrAI 43 CACCGGCG C[GA]^CCGG[CT]G StyI 72 CCATGG C^C[AT][AT]GG TfiI 261 GAATC G^A[AT]TC Tsp509I 279 AATT ^AATT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 558 CGCG CG^CG 677 CGCG AfaI 549 GTAC GT^AC 654 GTAC AlwI 714 GATCC GGATC(4/5) 623 GGATC AlwNI 58 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 442 CAGGTGCTG AvaII 126 GGTCC G^G[AT]CC 785 GGTCC BbsI 372 GTCTTC GAAGAC(2/6) 724 GTCTTC BcnI 510 CCGGG CC^[GC]GG 540 CCGGG BpmI 55 CTCCAG CTGGAG(16/14) 520 CTGGAG BpuAI 372 GTCTTC GAAGAC(2/6) 724 GTCTTC BsaJI 72 CCATGG C^C[ACGT][ACGT]GG 597 CCAGGG BseRI 589 CTCCTC GAGGAG(10/8) 218 GAGGAG BsmAI 240 GAGAC GTCTC(1/5) 544 GAGAC BsmFI 92 GGGAC GGGAC(10/14) 777 GTCCC BsrFI 44 ACCGGC [GA]^CCGG[CT] 361 ACCGGC BstUI 558 CGCG CG^CG 677 CGCG BstYI 566 AGATCT [GA]^GATC[CT] 713 AGATCC Cac8I 77 GCGTGC GC[ACGT]^[ACGT]GC 104 GCTTGC Cfr10I 44 ACCGGC [GA]^CCGG[CT] 361 ACCGGC DdeI 354 CTCAG C^T[ACGT]AG 734 CTGAG EaeI 507 CGGCCG [CT]^GGCC[GA] 647 TGGCCA HaeII 47 GGCGCT [GA]GCGC^[CT] 101 AGCGCT MaeII 95 ACGT A^CGT 547 ACGT MaeIII 223 GTCAC ^GT[ACGT]AC 581 GTCAC MflI 566 AGATCT [GA]^GATC[CT] 713 AGATCC MseI 179 TTAA T^TAA 407 TTAA MvnI 558 CGCG CG^CG 677 CGCG NciI 510 CCGGG CC^[GC]GG 540 CCGGG RsaI 549 GTAC GT^AC 654 GTAC Tru9I 179 TTAA T^TAA 407 TTAA Tsp45I 223 GTCAC ^GT[GC]AC 581 GTCAC XcmI 245 CCATTTATGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 515 CCATCCTGGAGATGG XhoII 566 AGATCT [GA]^GATC[CT] 713 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI BbvI BsiYI BslI BsrI BssKI BstF5I BstNI CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI TaqI TseI TspRI TthHB8I