Restriction map for
pFN21AE6269
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MslI MunI MvnI NarI
NciI NcoI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 13 GAATTT [GA]^AATT[CT]
AluI 332 AGCT AG^CT
AlwI 665 GGATC GGATC(4/5)
AocI 379 CCTGAGG CC^T[ACGT]AGG
ApoI 13 GAATTT [GA]^AATT[CT]
AvaII 628 GGACC G^G[AT]CC
BpmI 596 CTGGAG CTGGAG(16/14)
BsaI 324 GGTCTC GGTCTC(1/5)
BsgI 85 GTGCAG GTGCAG(16/14)
BsmAI 325 GTCTC GTCTC(1/5)
BsmI 396 GAATGC GAATGC(1/-1)
BsrDI 20 GCAATG GCAATG(2/0)
BsrFI 183 GCCGGC [GA]^CCGG[CT]
BssSI 214 CTCGTG CACGAG(-5/-1)
BstYI 665 GGATCT [GA]^GATC[CT]
Bsu36I 379 CCTGAGG CC^T[ACGT]AGG
Cfr10I 183 GCCGGC [GA]^CCGG[CT]
DdeI 380 CTGAG C^T[ACGT]AG
EarI 121 CTCTTC CTCTTC(1/4)
Eco81I 379 CCTGAGG CC^T[ACGT]AGG
EcoRV 571 GATATC GAT^ATC
HapII 184 CCGG C^CGG
HgaI 477 GACGC GACGC(5/10)
HpaII 184 CCGG C^CGG
Ksp632I 121 CTCTTC CTCTTC(1/4)
MflI 665 GGATCT [GA]^GATC[CT]
MspA1I 331 CAGCTG C[AC]G^C[GT]G
MspI 184 CCGG C^CGG
NaeI 183 GCCGGC GCC^GGC
NgoMIV 183 GCCGGC G^CCGGC
PleI 375 GAGTC GAGTC(4/5)
PstI 178 CTGCAG CTGCA^G
PvuII 331 CAGCTG CAG^CTG
SexAI 299 ACCTGGT A^CC[AT]GGT
SfcI 178 CTGCAG C^T[GA][CT]AG
StuI 291 AGGCCT AGG^CCT
TaqI 101 TCGA T^CGA
TfiI 609 GAATC G^A[AT]TC
Tsp509I 14 AATT ^AATT
TthHB8I 101 TCGA T^CGA
XhoII 665 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 72 GTAC GT^AC
318 GTAC
AvaI 337 CTCGGG C^[CT]CG[GA]G
349 CTCGGG
BalI 362 TGGCCA TGG^CCA
641 TGGCCA
BanI 137 GGTGCC G^G[CT][GA]CC
505 GGTGCC
BbsI 519 GAAGAC GAAGAC(2/6)
738 GAAGAC
BpuAI 519 GAAGAC GAAGAC(2/6)
738 GAAGAC
BseRI 310 CTCCTC GAGGAG(10/8)
382 GAGGAG
BsmFI 160 GGGAC GGGAC(10/14)
267 GTCCC
BsoBI 337 CTCGGG C^[CT]CG[GA]G
349 CTCGGG
DsaI 195 CCACGG C^C[GA][CT]GG
566 CCGTGG
EaeI 362 TGGCCA [CT]^GGCC[GA]
641 TGGCCA
EcoT14I 359 CCTTGG C^C[AT][AT]GG
365 CCAAGG
EcoT22I 398 ATGCAT ATGCA^T
498 ATGCAT
FauI 613 CCCGC CCCGC(4/6)
743 CCCGC
HinfI 375 GAGTC G^A[ACGT]TC
609 GAATC
MaeIII 418 GTGAC ^GT[ACGT]AC
562 GTCAC
MluNI 362 TGGCCA TGG^CCA
641 TGGCCA
MscI 362 TGGCCA TGG^CCA
641 TGGCCA
NsiI 398 ATGCAT ATGCA^T
498 ATGCAT
PflMI 195 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCAACCACTGG
RsaI 72 GTAC GT^AC
318 GTAC
StyI 359 CCTTGG C^C[AT][AT]GG
365 CCAAGG
Tsp45I 418 GTGAC ^GT[GC]AC
562 GTCAC
Van91I 195 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCAACCACTGG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI ApaI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HphI ItaI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI