(Circular) (Six-base) MAPSORT of: pFN21AE6269.seq Check: 9764 from: 1 to: 5470 pFN21AE6269 5470 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2049 2434 2049 Size: 385 5085 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3280 278 Size: 53 83 186 936 1744 2468 Fragments arranged by size: 2468 1744 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2813 1937 Size: 876 4594 Acc65I G'GTAC_C Cuts at: 2791 2791 Size: 5470 AclI AA'CG_TT Cuts at: 1563 3597 3970 1563 Size: 2034 373 3063 Fragments arranged by size: 3063 2034 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2780 3533 4917 5185 1632 Size: 1148 753 1384 268 1917 Fragments arranged by size: 1917 1384 1148 753 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5273 AflIII A'CryG_T Cuts at: 1203 4369 5346 1203 Size: 3166 977 1327 Fragments arranged by size: 3166 1327 977 AgeI A'CCGG_T Cuts at: 4301 4301 Size: 5470 AhdI GACnn_n'nnGTC Cuts at: 4199 4199 Size: 5470 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5470 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5470 AlwNI CAG_nnn'CTG Cuts at: 3213 4785 3213 Size: 1572 3898 ApaI G_GGCC'C Cuts at: 2537 2594 2687 2537 Size: 57 93 5320 Fragments arranged by size: 5320 93 57 ApaLI G'TGCA_C Cuts at: 3527 4683 3527 Size: 1156 4314 ApoI r'AATT_y Cuts at: 1493 2018 2779 3005 3157 5266 1493 Size: 525 761 226 152 2109 1697 Fragments arranged by size: 2109 1697 761 525 226 152 AseI AT'TA_AT Cuts at: 160 1032 4024 160 Size: 872 2992 1606 Fragments arranged by size: 2992 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5470 AvaI C'yCGr_G Cuts at: 1870 1943 2342 2354 2766 2795 1870 Size: 73 399 12 412 29 4545 Fragments arranged by size: 4545 412 399 73 29 12 BamHI G'GATC_C Cuts at: 2800 3167 2800 Size: 367 5103 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2510 2791 4246 618 Size: 325 217 627 355 368 281 1455 1842 Fragments arranged by size: 1842 1455 627 368 355 325 281 217 BanII G_rGCy'C Cuts at: 1391 2120 2220 2537 2594 2687 2789 1391 Size: 729 100 317 57 93 102 4072 Fragments arranged by size: 4072 729 317 102 100 93 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2531 2750 928 Size: 971 632 219 3648 Fragments arranged by size: 3648 971 632 219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3661 3695 1903 Size: 34 1724 34 3678 Fragments arranged by size: 3678 1724 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3362 4572 3362 Size: 1210 4260 BclI T'GATC_A Cuts at: 1548 1548 Size: 5470 BfrBI ATG'CAT Cuts at: 2405 2505 2405 Size: 100 5370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4081 136 Size: 107 122 71 631 605 2409 1525 Fragments arranged by size: 2409 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5465 5465 Size: 5470 BlpI GC'TnA_GC Cuts at: 2887 2887 Size: 5470 Bme1580I G_kGCm'C Cuts at: 1790 2537 2594 2687 3531 4687 1790 Size: 747 57 93 844 1156 2573 Fragments arranged by size: 2573 1156 844 747 93 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 2335 3201 Size: 416 53 83 399 24 767 151 866 Cuts at: 3201 4159 442 Size: 958 1753 Fragments arranged by size: 1753 958 866 767 416 399 151 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5470 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2622 4130 1239 Size: 1383 1508 2579 Fragments arranged by size: 2579 1508 1383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3595 4460 4758 3595 Size: 865 298 4307 Fragments arranged by size: 4307 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2335 4133 882 Size: 617 836 1798 2219 Fragments arranged by size: 2219 1798 836 617 BsaAI yAC'GTr Cuts at: 493 1204 3261 493 Size: 711 2057 2702 Fragments arranged by size: 2702 2057 711 BsaBI GATnn'nnATC Cuts at: 5464 5464 Size: 5470 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3277 3659 4294 275 Size: 53 83 186 936 1744 382 635 1451 Fragments arranged by size: 1744 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3903 4301 4575 4722 5133 3903 Size: 398 274 147 411 4240 Fragments arranged by size: 4240 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5245 Fragments arranged by size: 5245 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2306 2402 1186 Size: 1120 96 4254 Fragments arranged by size: 4254 1120 96 BseYI C'CCAG_C Cuts at: 2113 2233 2585 2680 4673 5169 2113 Size: 120 352 95 1993 496 2414 Fragments arranged by size: 2414 1993 496 352 120 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 1163 Size: 573 375 4522 Fragments arranged by size: 4522 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2920 3681 4709 1540 Size: 275 186 919 761 1028 2301 Fragments arranged by size: 2301 1028 919 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3531 3616 4687 3531 Size: 85 1071 4314 Fragments arranged by size: 4314 1071 85 BsmI GAATG_Cn' Cuts at: 2407 2981 3074 2407 Size: 574 93 4803 Fragments arranged by size: 4803 574 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2220 2537 2594 2687 2789 3531 Size: 399 330 100 317 57 93 102 742 Cuts at: 3531 3616 4687 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 317 102 100 93 85 57 BspEI T'CCGG_A Cuts at: 5133 5133 Size: 5470 BspHI T'CATG_A Cuts at: 3359 5089 3359 Size: 1730 3740 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2434 2644 2825 844 Size: 1205 385 210 181 3489 Fragments arranged by size: 3489 1205 385 210 181 BsrBI CCG'CTC Cuts at: 1394 1823 3357 1394 Size: 429 1534 3507 Fragments arranged by size: 3507 1534 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2032 3965 4139 65 Size: 1183 784 1933 174 1396 Fragments arranged by size: 1933 1396 1183 784 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2188 4114 4301 1700 Size: 110 378 1926 187 2869 Fragments arranged by size: 2869 1926 378 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5470 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5470 BssSI C'ACGA_G Cuts at: 2219 3530 4542 2219 Size: 1311 1012 3147 Fragments arranged by size: 3147 1311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5470 BstBI TT'CG_AA Cuts at: 4274 4274 Size: 5470 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5470 BstYI r'GATC_y Cuts at: 1972 2670 2800 3167 3552 3569 5021 5107 5119 Size: 698 130 367 385 17 1452 86 12 Cuts at: 5119 5465 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 698 385 367 346 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2385 2385 Size: 5470 BtgI C'CryG_G Cuts at: 513 1065 2200 2571 513 Size: 552 1135 371 3412 Fragments arranged by size: 3412 1135 552 371 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4731 Fragments arranged by size: 4731 683 56 BtsI GCAGTG_nn' Cuts at: 2986 3780 3800 2986 Size: 794 20 4656 Fragments arranged by size: 4656 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5435 DraI TTT'AAA Cuts at: 2774 2774 Size: 5470 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4477 809 Size: 507 3161 1802 Fragments arranged by size: 3161 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 2646 2917 Size: 54 1425 50 275 18 537 279 271 Cuts at: 2917 3806 5435 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 537 279 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2917 1537 Size: 275 1105 4090 Fragments arranged by size: 4090 1105 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 3400 1353 Size: 779 1268 3423 Fragments arranged by size: 3423 1268 779 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4057 4431 4577 387 Size: 1064 138 2468 374 146 1280 Fragments arranged by size: 2468 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2622 2780 3533 4130 4917 5185 1239 Size: 393 990 158 753 597 787 268 1524 Fragments arranged by size: 1524 990 787 753 597 393 268 158 EcoO109I rG'GnC_Cy Cuts at: 2533 2534 2590 2591 2533 Size: 1 56 1 5412 Fragments arranged by size: 5412 56 1 1 EcoRI G'AATT_C Cuts at: 2779 3157 2779 Size: 378 5092 EcoRV GAT'ATC Cuts at: 2578 2578 Size: 5470 FspI TGC'GCA Cuts at: 3976 3976 Size: 5470 HaeII r_GCGC'y Cuts at: 1678 4617 1678 Size: 2939 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4111 4143 4185 4217 1373 Size: 32 135 32 2539 32 42 32 2626 Fragments arranged by size: 2626 2539 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2814 3060 669 Size: 988 281 876 246 3079 Fragments arranged by size: 3079 988 876 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5470 HpaI GTT'AAC Cuts at: 3060 3060 Size: 5470 KpnI G_GTAC'C Cuts at: 2795 2795 Size: 5470 MfeI C'AATT_G Cuts at: 3069 3069 Size: 5470 MluI A'CGCG_T Cuts at: 5346 5346 Size: 5470 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2689 4584 4768 1305 Size: 1384 1895 184 2007 Fragments arranged by size: 2007 1895 1384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 2648 10 Size: 54 1425 343 537 279 2832 Fragments arranged by size: 2832 1425 537 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 3428 3787 3946 518 Size: 648 2262 359 159 2042 Fragments arranged by size: 2262 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2338 2886 3563 4711 4956 1606 Size: 732 548 677 1148 245 2120 Fragments arranged by size: 2120 1148 732 677 548 245 NaeI GCC'GGC Cuts at: 2190 2190 Size: 5470 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4918 NdeI CA'TA_TG Cuts at: 387 387 Size: 5470 NgoMIV G'CCGG_C Cuts at: 2188 2188 Size: 5470 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5470 NotI GC'GGCC_GC Cuts at: 2917 2917 Size: 5470 NsiI A_TGCA'T Cuts at: 2407 2507 2407 Size: 100 5370 NspI r_CATG'y Cuts at: 1132 1229 2828 4373 1132 Size: 97 1599 1545 2229 Fragments arranged by size: 2229 1599 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5470 PciI A'CATG_T Cuts at: 4369 4369 Size: 5470 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 2655 1108 Size: 1098 449 3923 Fragments arranged by size: 3923 1098 449 PfoI T'CCnGG_A Cuts at: 2430 2430 Size: 5470 PmeI GTTT'AAAC Cuts at: 2774 2774 Size: 5470 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2378 2410 3539 3571 5052 5084 2378 Size: 32 1129 32 1481 32 2764 Fragments arranged by size: 2764 1481 1129 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5470 PsiI TTA'TAA Cuts at: 3040 3040 Size: 5470 PspOMI G'GGCC_C Cuts at: 2533 2590 2683 2533 Size: 57 93 5320 Fragments arranged by size: 5320 93 57 PstI C_TGCA'G Cuts at: 830 2187 2822 830 Size: 1357 635 3478 Fragments arranged by size: 3478 1357 635 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5470 PvuII CAG'CTG Cuts at: 2338 2338 Size: 5470 SalI G'TCGA_C Cuts at: 1936 2812 1936 Size: 876 4594 SbfI CC_TGCA'GG Cuts at: 2822 2822 Size: 5470 ScaI AGT'ACT Cuts at: 3718 5430 3718 Size: 1712 3758 SexAI A'CCwGG_T Cuts at: 2304 2304 Size: 5470 SfcI C'TryA_G Cuts at: 826 1045 2183 2818 3953 4634 4825 826 Size: 219 1138 635 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 681 635 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5470 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5470 SmaI CCC'GGG Cuts at: 1872 2797 1872 Size: 925 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3574 4475 4737 820 Size: 197 926 1631 901 262 1553 Fragments arranged by size: 1631 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3261 493 Size: 2768 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5470 SphI G_CATG'C Cuts at: 1132 2828 1132 Size: 1696 3774 SspI AAT'ATT Cuts at: 5 52 3394 5 Size: 47 3342 2081 Fragments arranged by size: 3342 2081 47 StuI AGG'CCT Cuts at: 1517 2298 1517 Size: 781 4689 StyI C'CwwG_G Cuts at: 513 1065 2364 2370 2909 513 Size: 552 1299 6 539 3074 Fragments arranged by size: 3074 1299 552 539 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2140 2498 3497 3514 3667 3852 Size: 1255 462 98 358 999 17 153 185 Cuts at: 3852 4297 325 Size: 445 1498 Fragments arranged by size: 1498 1255 999 462 445 358 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3716 5428 96 Size: 274 80 33 51 1443 1739 1712 138 Fragments arranged by size: 1739 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2230 2262 2625 2657 5430 5462 2230 Size: 32 363 32 2773 32 2238 Fragments arranged by size: 2773 2238 363 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5470 XbaI T'CTAG_A Cuts at: 2806 2806 Size: 5470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5470 XmaI C'CCGG_G Cuts at: 1870 2795 1870 Size: 925 4545 XmnI GAAnn'nnTTC Cuts at: 3599 3599 Size: 5470 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3278 276 Size: 53 83 186 936 1744 2468 Fragments arranged by size: 2468 1744 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI