Restriction map for
pFN21AE3486
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
BbeI BfrI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BsrFI BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Bsu36I
Cfr10I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FseI Hin1I HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1502 GTCGAC GT^[AC][GT]AC
Asp700I 4976 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 150 TGCGCA TGC^GCA
AvrII 556 CCTAGG C^CTAGG
BamHI 3778 GGATCC G^GATCC
BanI 1395 GGTGCC G^G[CT][GA]CC
BbrPI 477 CACGTG CAC^GTG
BbvCI 2558 CCTCAGC CCTCAGC(-5/-2)
BcgI 397 GCAATGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4629 TGATCA T^GATCA
BcnI 1726 CCGGG CC^[GC]GG
BglI 352 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 556 CCTAGG C^CTAGG
BlpI 5156 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5156 GCTCAGC GC^T[ACGT]AGC
BsaAI 477 CACGTG [CT]AC^GT[GA]
BsgI 1858 GTGCAG GTGCAG(16/14)
BspDI 2261 ATCGAT AT^CGAT
BspLU11I 883 ACATGT A^CATGT
BssSI 4376 CTCGTG CACGAG(-5/-1)
BstAPI 1318 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 5156 GCTCAGC GC^T[ACGT]AGC
ClaI 2261 ATCGAT AT^CGAT
DsaI 1599 CCGTGG C^C[GA][CT]GG
FauI 1780 CCCGC CCCGC(4/6)
FbaI 4629 TGATCA T^GATCA
FspI 150 TGCGCA TGC^GCA
HaeII 2039 GGCGCT [GA]GCGC^[CT]
HapII 1726 CCGG C^CGG
HindIII 2701 AAGCTT A^AGCTT
HpaII 1726 CCGG C^CGG
MspI 1726 CCGG C^CGG
NciI 1726 CCGGG CC^[GC]GG
PmaCI 477 CACGTG CAC^GTG
PmlI 477 CACGTG CAC^GTG
SacI 3813 GAGCTC GAGCT^C
SalI 1502 GTCGAC G^TCGAC
ScaI 4828 AGTACT AGT^ACT
SexAI 1391 ACCAGGT A^CC[AT]GGT
XmnI 4976 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 318 CGCG CG^CG
3912 CGCG
BalI 414 TGGCCA TGG^CCA
1767 TGGCCA
BanII 61 GGGCTC G[GA]GC[CT]^C
3813 GAGCTC
BsaBI 2306 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4684 GATAAAAATC
BsaI 1038 GAGACC GGTCTC(1/5)
2456 GAGACC
BspHI 593 TCATGA T^CATGA
4626 TCATGA
BsrBI 1367 CCGCTC CCGCTC(-3/-3)
2160 CCGCTC
BsrDI 397 GCAATG GCAATG(2/0)
628 GCAATG
BstUI 318 CGCG CG^CG
3912 CGCG
DraI 2992 TTTAAA TTT^AAA
4318 TTTAAA
EcoRV 1083 GATATC GAT^ATC
2255 GATATC
MamI 2306 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4684 GATAAAAATC
MluNI 414 TGGCCA TGG^CCA
1767 TGGCCA
MscI 414 TGGCCA TGG^CCA
1767 TGGCCA
MslI 898 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5256 CACTGAAGTG
MvnI 318 CGCG CG^CG
3912 CGCG
PstI 136 CTGCAG CTGCA^G
1316 CTGCAG
PvuII 1313 CAGCTG CAG^CTG
3917 CAGCTG
RcaI 593 TCATGA T^CATGA
4626 TCATGA
XbaI 2242 TCTAGA T^CTAGA
4864 TCTAGA
XcmI 3782 CCAGCTCAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5109 CCACAGTCCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspA1I
MunI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII