(Linear) (Six-base) MAPSORT of: KIEE3486.seq Check: 7839 from: 1 to: 5376 KIEE3486.orf With 182 enzymes: SgfI * July 3, 2012 17:58 .. AccI GT'mk_AC Cuts at: 0 1503 5376 Size: 1503 3873 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 398 3473 3794 3866 3941 4004 4955 Size: 383 15 3075 321 72 75 63 951 Cuts at: 4955 5279 5376 Size: 324 97 Fragments arranged by size: 3075 951 383 324 321 97 75 72 63 15 AflIII A'CryG_T Cuts at: 0 476 478 883 892 2128 5376 Size: 476 2 405 9 1236 3248 Fragments arranged by size: 3248 1236 476 405 9 2 AleI CACnn'nnGTG Cuts at: 0 5260 5376 Size: 5260 116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3850 5376 Size: 3850 1526 AlwNI CAG_nnn'CTG Cuts at: 0 836 1222 1822 3004 5257 5376 Size: 836 386 600 1182 2253 119 Fragments arranged by size: 2253 1182 836 600 386 119 ApoI r'AATT_y Cuts at: 0 19 198 221 578 937 1918 2613 3596 Size: 19 179 23 357 359 981 695 983 Cuts at: 3596 4115 4123 4314 5376 Size: 519 8 191 1062 Fragments arranged by size: 1062 983 981 695 519 359 357 191 179 23 19 8 AvrII C'CTAG_G Cuts at: 0 556 5376 Size: 556 4820 BamHI G'GATC_C Cuts at: 0 3778 5376 Size: 3778 1598 BanI G'GyrC_C Cuts at: 0 1395 5376 Size: 1395 3981 BanII G_rGCy'C Cuts at: 0 65 3817 5376 Size: 65 3752 1559 Fragments arranged by size: 3752 1559 65 BbsI GAAGACnn'nnnn_ Cuts at: 0 1166 1199 2648 3208 3476 5376 Size: 1166 33 1449 560 268 1900 Fragments arranged by size: 1900 1449 1166 560 268 33 BbvCI CC'TCA_GC Cuts at: 0 2559 5376 Size: 2559 2817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 386 420 5376 Size: 386 34 4956 Fragments arranged by size: 4956 386 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1257 1703 4094 5376 Size: 1257 446 2391 1282 Fragments arranged by size: 2391 1282 1257 446 BclI T'GATC_A Cuts at: 0 4629 5376 Size: 4629 747 BglI GCCn_nnn'nGGC Cuts at: 0 358 5376 Size: 358 5018 BglII A'GATC_T Cuts at: 0 1075 2116 2245 2311 3151 5376 Size: 1075 1041 129 66 840 2225 Fragments arranged by size: 2225 1075 1041 840 129 66 BlpI GC'TnA_GC Cuts at: 0 5157 5376 Size: 5157 219 BmgBI CAC'GTC Cuts at: 0 893 4137 5376 Size: 893 3244 1239 Fragments arranged by size: 3244 1239 893 BmrI ACTGGGnnnn_n' Cuts at: 0 3653 4820 4842 5376 Size: 3653 1167 22 534 Fragments arranged by size: 3653 1167 534 22 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3173 5376 Size: 3173 2203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1199 1643 1787 1799 2561 2567 2756 3842 Size: 1199 444 144 12 762 6 189 1086 Cuts at: 3842 5376 Size: 1534 Fragments arranged by size: 1534 1199 1086 762 444 189 144 12 6 Bpu10I CC'TnA_GC Cuts at: 0 1997 2559 5376 Size: 1997 562 2817 Fragments arranged by size: 2817 1997 562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1619 2303 2345 4593 5376 Size: 1619 684 42 2248 783 Fragments arranged by size: 2248 1619 783 684 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 1032 2450 5376 Size: 1032 1418 2926 Fragments arranged by size: 2926 1418 1032 BsaAI yAC'GTr Cuts at: 0 479 5376 Size: 479 4897 BsaBI GATnn'nnATC Cuts at: 0 2310 4688 5376 Size: 2310 2378 688 Fragments arranged by size: 2378 2310 688 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1703 1733 5376 Size: 1703 30 3643 Fragments arranged by size: 3643 1703 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 736 1001 1341 1590 1691 1727 1742 1790 Size: 736 265 340 249 101 36 15 48 Cuts at: 1790 2306 3146 3205 5376 Size: 516 840 59 2171 Fragments arranged by size: 2171 840 736 516 340 265 249 101 59 48 36 15 BseYI C'CCAG_C Cuts at: 0 335 1137 1511 1783 5086 5098 5376 Size: 335 802 374 272 3303 12 278 Fragments arranged by size: 3303 802 374 335 278 272 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1879 5376 Size: 1879 3497 BsiHKAI G_wGCw'C Cuts at: 0 574 881 1337 2547 3817 5376 Size: 574 307 456 1210 1270 1559 Fragments arranged by size: 1559 1270 1210 574 456 307 Bsp1286I G_dGCh'C Cuts at: 0 65 574 881 1337 2547 3817 5376 Size: 65 509 307 456 1210 1270 1559 Fragments arranged by size: 1559 1270 1210 509 456 307 65 BspHI T'CATG_A Cuts at: 0 593 4626 5376 Size: 593 4033 750 Fragments arranged by size: 4033 750 593 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 143 3117 4041 5376 Size: 143 2974 924 1335 Fragments arranged by size: 2974 1335 924 143 BsrBI CCG'CTC Cuts at: 0 1369 2162 5376 Size: 1369 793 3214 Fragments arranged by size: 3214 1369 793 BsrDI GCAATG_nn' Cuts at: 0 404 635 5376 Size: 404 231 4741 Fragments arranged by size: 4741 404 231 BssSI C'ACGA_G Cuts at: 0 4376 5376 Size: 4376 1000 BstAPI GCAn_nnn'nTGC Cuts at: 0 1324 5376 Size: 1324 4052 BstEII G'GTnAC_C Cuts at: 0 1387 2186 2276 5376 Size: 1387 799 90 3100 Fragments arranged by size: 3100 1387 799 90 BstYI r'GATC_y Cuts at: 0 609 1075 1098 2116 2245 2311 3073 3151 Size: 609 466 23 1018 129 66 762 78 Cuts at: 3151 3778 5376 Size: 627 1598 Fragments arranged by size: 1598 1018 762 627 609 466 129 78 66 23 BtgI C'CryG_G Cuts at: 0 1599 5376 Size: 1599 3777 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 298 5376 Size: 298 5078 BtsI GCAGTG_nn' Cuts at: 0 5154 5376 Size: 5154 222 ClaI AT'CG_AT Cuts at: 0 2262 5376 Size: 2262 3114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1670 1705 3776 3811 5376 Size: 1670 35 2071 35 1565 Fragments arranged by size: 2071 1670 1565 35 35 DraI TTT'AAA Cuts at: 0 2994 4320 5376 Size: 2994 1326 1056 Fragments arranged by size: 2994 1326 1056 EaeI y'GGCC_r Cuts at: 0 414 1767 4657 5376 Size: 414 1353 2890 719 Fragments arranged by size: 2890 1353 719 414 EarI CTCTTCn'nnn_ Cuts at: 0 318 1373 1560 1943 2199 4544 4661 4955 Size: 318 1055 187 383 256 2345 117 294 Cuts at: 4955 5376 Size: 421 Fragments arranged by size: 2345 1055 421 383 318 294 256 187 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1685 5376 Size: 1685 3691 EcoICRI GAG'CTC Cuts at: 0 3815 5376 Size: 3815 1561 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 398 1199 1643 1787 1799 2561 2567 Size: 383 15 801 444 144 12 762 6 Cuts at: 2567 2756 3473 3794 3842 3866 3941 4004 4955 Size: 189 717 321 48 24 75 63 951 Cuts at: 4955 5279 5376 Size: 324 97 Fragments arranged by size: 951 801 762 717 444 383 324 321 189 144 97 75 63 48 24 15 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 230 1802 3113 4568 5376 Size: 230 1572 1311 1455 808 Fragments arranged by size: 1572 1455 1311 808 230 EcoO109I rG'GnC_Cy Cuts at: 0 3118 3578 4989 5376 Size: 3118 460 1411 387 Fragments arranged by size: 3118 1411 460 387 EcoRI G'AATT_C Cuts at: 0 198 221 578 1918 2613 5376 Size: 198 23 357 1340 695 2763 Fragments arranged by size: 2763 1340 695 357 198 23 EcoRV GAT'ATC Cuts at: 0 1085 2257 5376 Size: 1085 1172 3119 Fragments arranged by size: 3119 1172 1085 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1120 5376 Size: 1120 4256 FspI TGC'GCA Cuts at: 0 152 5376 Size: 152 5224 HaeII r_GCGC'y Cuts at: 0 2043 5376 Size: 2043 3333 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 339 371 712 744 882 914 1062 1094 Size: 339 32 341 32 138 32 148 32 Cuts at: 1094 1436 1468 2965 2997 3340 3372 3466 3498 Size: 342 32 1497 32 343 32 94 32 Cuts at: 3498 3697 3729 3964 3985 3996 4017 4507 4539 Size: 199 32 235 21 11 21 490 32 Cuts at: 4539 4660 4692 5376 Size: 121 32 684 Fragments arranged by size: 1497 684 490 343 342 341 339 235 199 148 138 121 94 32 32 32 32 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 0 1504 2448 3710 5376 Size: 1504 944 1262 1666 Fragments arranged by size: 1666 1504 1262 944 HindIII A'AGCT_T Cuts at: 0 2701 5376 Size: 2701 2675 MfeI C'AATT_G Cuts at: 0 302 686 1355 5376 Size: 302 384 669 4021 Fragments arranged by size: 4021 669 384 302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 913 1547 4211 5376 Size: 913 634 2664 1165 Fragments arranged by size: 2664 1165 913 634 MscI TGG'CCA Cuts at: 0 416 1769 5376 Size: 416 1353 3607 Fragments arranged by size: 3607 1353 416 MslI CAynn'nnrTG Cuts at: 0 902 5260 5376 Size: 902 4358 116 Fragments arranged by size: 4358 902 116 MspA1I CmG'CkG Cuts at: 0 1315 1783 3919 5376 Size: 1315 468 2136 1457 Fragments arranged by size: 2136 1457 1315 468 NdeI CA'TA_TG Cuts at: 0 258 681 2415 5376 Size: 258 423 1734 2961 Fragments arranged by size: 2961 1734 423 258 NspI r_CATG'y Cuts at: 0 887 4717 5251 5376 Size: 887 3830 534 125 Fragments arranged by size: 3830 887 534 125 PciI A'CATG_T Cuts at: 0 883 5376 Size: 883 4493 PflMI CCAn_nnn'nTGG Cuts at: 0 529 1498 1783 2779 5376 Size: 529 969 285 996 2597 Fragments arranged by size: 2597 996 969 529 285 PfoI T'CCnGG_A Cuts at: 0 863 2772 5376 Size: 863 1909 2604 Fragments arranged by size: 2604 1909 863 PmlI CAC'GTG Cuts at: 0 479 5376 Size: 479 4897 PpuMI rG'GwC_Cy Cuts at: 0 3118 3578 4989 5376 Size: 3118 460 1411 387 Fragments arranged by size: 3118 1411 460 387 PsiI TTA'TAA Cuts at: 0 17 265 2525 4045 5376 Size: 17 248 2260 1520 1331 Fragments arranged by size: 2260 1520 1331 248 17 PstI C_TGCA'G Cuts at: 0 140 1320 5376 Size: 140 1180 4056 Fragments arranged by size: 4056 1180 140 PvuII CAG'CTG Cuts at: 0 1315 3919 5376 Size: 1315 2604 1457 Fragments arranged by size: 2604 1457 1315 SacI G_AGCT'C Cuts at: 0 3817 5376 Size: 3817 1559 SalI G'TCGA_C Cuts at: 0 1502 5376 Size: 1502 3874 ScaI AGT'ACT Cuts at: 0 4830 5376 Size: 4830 546 SexAI A'CCwGG_T Cuts at: 0 1391 5376 Size: 1391 3985 SfcI C'TryA_G Cuts at: 0 136 665 1316 1584 1923 2067 2888 4679 Size: 136 529 651 268 339 144 821 1791 Cuts at: 4679 5376 Size: 697 Fragments arranged by size: 1791 821 697 651 529 339 268 144 136 SmlI C'TyrA_G Cuts at: 0 1634 2318 2360 4608 5376 Size: 1634 684 42 2248 768 Fragments arranged by size: 2248 1634 768 684 42 SspI AAT'ATT Cuts at: 0 1307 2383 2521 3099 5376 Size: 1307 1076 138 578 2277 Fragments arranged by size: 2277 1307 1076 578 138 StyI C'CwwG_G Cuts at: 0 556 1178 1840 4130 4543 5376 Size: 556 622 662 2290 413 833 Fragments arranged by size: 2290 833 662 622 556 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1151 5376 Size: 1151 4225 TatI w'GTAC_w Cuts at: 0 3661 4057 4828 5376 Size: 3661 396 771 548 Fragments arranged by size: 3661 771 548 396 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 564 596 1449 1481 1703 1735 3065 3097 Size: 564 32 853 32 222 32 1330 32 Cuts at: 3097 5376 Size: 2279 Fragments arranged by size: 2279 1330 853 564 222 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 2242 4864 5376 Size: 2242 2622 512 Fragments arranged by size: 2622 2242 512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3789 5116 5376 Size: 3789 1327 260 Fragments arranged by size: 3789 1327 260 XmnI GAAnn'nnTTC Cuts at: 0 4980 5376 Size: 4980 396 Enzymes that do cut: AccI AcuI AflIII AleI AlfI AlwNI ApoI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NdeI NspI PciI PflMI PfoI PmlI PpuMI PsiI PstI PvuII SacI SalI ScaI SexAI SfcI SmlI SspI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BbeI BfrBI Bme1580I BmtI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I DraIII DrdI EagI FseI FspAI HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI ZraI