################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## Acc65I AccI AccII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbvCI BciVI BclI BfrI BlnI BseRI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccIII 614 TCCGGA T^CCGGA AflIII 59 ACACGT A^C[GA][CT]GT Alw44I 1080 GTGCAC G^TGCAC ApaLI 1080 GTGCAC G^TGCAC AspHI 1080 GTGCAC G[AT]GC[AT]^C AspI 913 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 4 CACGTG CAC^GTG BcgI 847 GCACTTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1571 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 367 CTGGAG CTGGAG(16/14) BsaI 1312 GAGACC GGTCTC(1/5) BseAI 614 TCCGGA T^CCGGA BsiEI 938 CGGCCG CG[GA][CT]^CG BsiHKAI 1080 GTGCAC G[AT]GC[AT]^C BspEI 614 TCCGGA T^CCGGA BsrBI 1432 GAGCGG CCGCTC(-3/-3) BstXI 1367 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1017 GACAGAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 938 CGGCCG C^GGCCG EarI 172 CTCTTC CTCTTC(1/4) EclXI 938 CGGCCG C^GGCCG Eco52I 938 CGGCCG C^GGCCG EcoNI 18 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 874 GAATTC G^AATTC HindIII 1048 AAGCTT A^AGCTT Ksp632I 172 CTCTTC CTCTTC(1/4) MroI 614 TCCGGA T^CCGGA NheI 298 GCTAGC G^CTAGC NspI 1325 GCATGT [GA]CATG^[CT] PflFI 913 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 477 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4 CACGTG CAC^GTG PmlI 4 CACGTG CAC^GTG SapI 171 GCTCTTC GCTCTTC(1/4) SbfI 660 CCTGCAGG CCTGCA^GG SmlI 222 CTTGAG C^T[CT][GA]AG Sse8387I 660 CCTGCAGG CCTGCA^GG Tth111I 913 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 477 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 1023 GACGTC GACGT^C 1885 GACGTC AcsI 874 GAATTC [GA]^AATT[CT] 1501 GAATTT AcyI 1023 GACGTC G[GA]^CG[CT]C 1885 GACGTC AlwNI 1822 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1876 CAGCCGCTG ApoI 874 GAATTC [GA]^AATT[CT] 1501 GAATTT BanI 687 GGTGCC G^G[CT][GA]CC 1840 GGCACC BbsI 910 GAAGAC GAAGAC(2/6) 1157 GTCTTC BfaI 299 CTAG C^TAG 703 CTAG BglII 545 AGATCT A^GATCT 1284 AGATCT BpuAI 910 GAAGAC GAAGAC(2/6) 1157 GTCTTC BsaAI 4 CACGTG [CT]AC^GT[GA] 60 CACGTA BsaHI 1023 GACGTC G[GA]^CG[CT]C 1885 GACGTC BsaWI 614 TCCGGA [AT]^CCGG[AT] 773 ACCGGA BsmBI 240 CGTCTC CGTCTC(1/5) 1021 GAGACG BspMI 17 ACCTGC ACCTGC(4/8) 1315 ACCTGC BssSI 961 CACGAG CACGAG(-5/-1) 1039 CTCGTG BstAPI 33 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1821 GCAGGCCCTGC BstYI 545 AGATCT [GA]^GATC[CT] 1284 AGATCT Hin1I 1023 GACGTC G[GA]^CG[CT]C 1885 GACGTC MaeI 299 CTAG C^TAG 703 CTAG MflI 545 AGATCT [GA]^GATC[CT] 1284 AGATCT MseI 520 TTAA T^TAA 1505 TTAA MspA1I 1690 CAGCGG C[AC]G^C[GT]G 1879 CCGCTG StuI 1151 AGGCCT AGG^CCT 1394 AGGCCT Tru9I 520 TTAA T^TAA 1505 TTAA Tsp45I 283 GTCAC ^GT[GC]AC 1124 GTGAC XcmI 1652 CCATCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1752 CCACACCAACTTTGG XhoII 545 AGATCT [GA]^GATC[CT] 1284 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AlwI ApaI AvaII BalI BanII BbvI BcnI BlpI BmyI Bpu1102I BsaBI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MamI MboI MboII MluNI MnlI MscI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp509I TspRI TthHB8I