(Circular) (Six-base) MAPSORT of: pFN21AE5729.seq Check: 8645 from: 1 to: 6625 pFN21AE5729 6625 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3032 3894 4435 278 Size: 53 83 186 936 1496 862 541 2468 Fragments arranged by size: 2468 1496 936 862 541 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2360 2909 3041 4688 6072 6340 1632 Size: 416 312 549 132 1647 1384 268 1917 Fragments arranged by size: 1917 1647 1384 549 416 312 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 2064 5524 6501 1203 Size: 861 3460 977 1327 Fragments arranged by size: 3460 1327 977 861 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6625 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2774 2806 2774 Size: 32 6593 AlwNI CAG_nnn'CTG Cuts at: 3832 3886 4368 5940 3832 Size: 54 482 1572 4517 Fragments arranged by size: 4517 1572 482 54 ApaI G_GGCC'C Cuts at: 2057 2142 3277 3813 2057 Size: 85 1135 536 4869 Fragments arranged by size: 4869 1135 536 85 ApaLI G'TGCA_C Cuts at: 3085 4682 5838 3085 Size: 1597 1156 3872 Fragments arranged by size: 3872 1597 1156 ApoI r'AATT_y Cuts at: 1493 2879 3506 3934 4160 4312 6421 1493 Size: 1386 627 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 627 428 226 152 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 3950 1870 Size: 73 2007 4545 Fragments arranged by size: 4545 2007 73 BamHI G'GATC_C Cuts at: 3955 4322 3955 Size: 367 6258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2692 3845 3946 5401 618 Size: 325 217 627 905 1153 101 1455 1842 Fragments arranged by size: 1842 1455 1153 905 627 325 217 101 BanII G_rGCy'C Cuts at: 1391 2057 2142 2769 3075 3277 3602 3706 3813 Size: 666 85 627 306 202 325 104 107 Cuts at: 3813 3944 1391 Size: 131 4072 Fragments arranged by size: 4072 666 627 325 306 202 131 107 104 85 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 3155 928 Size: 971 1023 233 4398 Fragments arranged by size: 4398 1023 971 233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2841 2875 4816 4850 1903 Size: 34 50 34 820 34 1941 34 3678 Fragments arranged by size: 3678 1941 820 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 1548 Size: 6625 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3582 5236 136 Size: 107 122 71 631 605 1910 1654 1525 Fragments arranged by size: 1910 1654 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2550 3289 6620 2550 Size: 739 3331 2555 Fragments arranged by size: 3331 2555 739 BlpI GC'TnA_GC Cuts at: 2396 2536 2763 4042 2396 Size: 140 227 1279 4979 Fragments arranged by size: 4979 1279 227 140 Bme1580I G_kGCm'C Cuts at: 1790 2057 2142 3089 3277 3813 4686 5842 1790 Size: 267 85 947 188 536 873 1156 2573 Fragments arranged by size: 2573 1156 947 873 536 267 188 85 BmgBI CAC'GTC Cuts at: 1318 2790 3424 1318 Size: 1472 634 4519 Fragments arranged by size: 4519 1472 634 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2602 2842 3280 Size: 416 53 83 399 24 1185 240 438 Cuts at: 3280 3352 3813 3816 4356 5314 442 Size: 72 461 3 540 958 1753 Fragments arranged by size: 1753 1185 958 540 461 438 416 399 240 83 72 53 24 3 BmtI G_CTAG'C Cuts at: 1056 2307 1056 Size: 1251 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 5285 1239 Size: 1154 2892 2579 Fragments arranged by size: 2892 2579 1154 Bpu10I CC'TnA_GC Cuts at: 2391 3464 2391 Size: 1073 5552 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2248 4750 5615 5913 2248 Size: 2502 865 298 2960 Fragments arranged by size: 2960 2502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3311 5288 882 Size: 617 1812 1977 2219 Fragments arranged by size: 2219 1977 1812 617 BsaAI yAC'GTr Cuts at: 493 1204 2011 2067 4416 493 Size: 711 807 56 2349 2702 Fragments arranged by size: 2702 2349 807 711 56 BsaBI GATnn'nnATC Cuts at: 3371 3635 3656 6619 3371 Size: 264 21 2963 3377 Fragments arranged by size: 3377 2963 264 21 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3029 3891 4432 4814 Size: 53 83 186 936 1496 862 541 382 Cuts at: 4814 5449 275 Size: 635 1451 Fragments arranged by size: 1496 1451 936 862 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2619 2778 5058 5456 5730 5877 6288 2619 Size: 159 2280 398 274 147 411 2956 Fragments arranged by size: 2956 2280 411 398 274 159 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3851 3881 1178 Size: 30 165 30 2448 30 3922 Fragments arranged by size: 3922 2448 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6625 BseYI C'CCAG_C Cuts at: 2232 5828 6324 2232 Size: 3596 496 2533 Fragments arranged by size: 3596 2533 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6052 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2946 4075 4836 5864 1540 Size: 275 186 945 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 945 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3089 4686 4771 5842 3089 Size: 1597 85 1071 3872 Fragments arranged by size: 3872 1597 1071 85 BsmI GAATG_Cn' Cuts at: 4136 4229 4136 Size: 93 6532 BsmBI CGTCTCn'nnnn_ Cuts at: 2251 3020 2251 Size: 769 5856 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2142 2769 3075 3089 3277 3602 Size: 399 267 85 627 306 14 188 325 Cuts at: 3602 3706 3813 3944 4686 4771 5842 1391 Size: 104 107 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 627 399 325 306 267 188 131 107 104 85 85 14 BspEI T'CCGG_A Cuts at: 2619 6288 2619 Size: 3669 2956 BspHI T'CATG_A Cuts at: 4514 6244 4514 Size: 1730 4895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 3329 3980 844 Size: 1187 1298 651 3489 Fragments arranged by size: 3489 1298 1187 651 BsrBI CCG'CTC Cuts at: 1394 1823 3439 4512 1394 Size: 429 1616 1073 3507 Fragments arranged by size: 3507 1616 1073 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2305 3277 3355 5120 5294 65 Size: 1183 1057 972 78 1765 174 1396 Fragments arranged by size: 1765 1396 1183 1057 972 174 78 BsrFI r'CCGG_y Cuts at: 1700 1810 5269 5456 1700 Size: 110 3459 187 2869 Fragments arranged by size: 3459 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 2966 3044 4685 5697 2966 Size: 78 1641 1012 3894 Fragments arranged by size: 3894 1641 1012 78 BstAPI GCAn_nnn'nTGC Cuts at: 794 2044 3832 794 Size: 1250 1788 3587 Fragments arranged by size: 3587 1788 1250 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstXI CCAn_nnnn'nTGG Cuts at: 3379 3379 Size: 6625 BstYI r'GATC_y Cuts at: 1972 2550 3289 3955 4322 4707 4724 6176 6262 Size: 578 739 666 367 385 17 1452 86 Cuts at: 6262 6274 6620 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 739 666 578 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2110 2271 2487 2563 2940 3232 3378 Size: 552 1045 161 216 76 377 292 146 Cuts at: 3378 3735 513 Size: 357 3403 Fragments arranged by size: 3403 1045 552 377 357 292 216 161 146 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 2207 3224 4141 4935 4955 2207 Size: 1017 917 794 20 3877 Fragments arranged by size: 3877 1017 917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 3929 3929 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3028 5632 809 Size: 507 1712 2604 1802 Fragments arranged by size: 2604 1802 1712 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2577 2862 2943 Size: 54 1425 50 275 18 747 285 81 Cuts at: 2943 3208 3574 3678 3738 4072 4961 6590 8 Size: 265 366 104 60 334 889 1629 43 Fragments arranged by size: 1629 1425 889 747 366 334 285 275 265 104 81 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2943 4072 1537 Size: 275 1131 1129 4090 Fragments arranged by size: 4090 1131 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 4555 1353 Size: 830 2372 3423 Fragments arranged by size: 3423 2372 830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5212 5586 5732 387 Size: 1064 138 3623 374 146 1280 Fragments arranged by size: 3623 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2360 2393 2909 3041 4688 5285 Size: 393 416 312 33 516 132 1647 597 Cuts at: 5285 6072 6340 1239 Size: 787 268 1524 Fragments arranged by size: 1647 1524 787 597 516 416 393 312 268 132 33 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2027 Size: 6625 EcoO109I rG'GnC_Cy Cuts at: 2032 2053 2054 2138 2220 2344 2604 3106 3810 Size: 21 1 84 82 124 260 502 704 Cuts at: 3810 3829 2032 Size: 19 4828 Fragments arranged by size: 4828 704 502 260 124 84 82 21 19 1 EcoRI G'AATT_C Cuts at: 2879 3934 4312 2879 Size: 1055 378 5192 Fragments arranged by size: 5192 1055 378 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 5772 1678 Size: 4094 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2960 2992 5266 5298 5340 Size: 32 135 32 1388 32 2274 32 42 Cuts at: 5340 5372 1373 Size: 32 2626 Fragments arranged by size: 2626 2274 1388 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3969 4215 669 Size: 988 281 2031 246 3079 Fragments arranged by size: 3079 2031 988 281 246 HindIII A'AGCT_T Cuts at: 748 3053 748 Size: 2305 4320 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5739 5923 1305 Size: 4434 184 2007 Fragments arranged by size: 4434 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 3210 3576 3680 10 Size: 54 1425 343 747 631 366 104 2955 Fragments arranged by size: 2955 1425 747 631 366 343 104 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2406 3329 3377 4583 4942 5101 Size: 648 843 397 923 48 1206 359 159 Cuts at: 5101 518 Size: 2042 Fragments arranged by size: 2042 1206 923 843 648 397 359 159 48 MspA1I CmG'CkG Cuts at: 1606 3697 3886 4041 4718 5866 6111 1606 Size: 2091 189 155 677 1148 245 2120 Fragments arranged by size: 2120 2091 1148 677 245 189 155 NcoI C'CATG_G Cuts at: 513 1065 2271 2487 3378 513 Size: 552 1206 216 891 3760 Fragments arranged by size: 3760 1206 891 552 216 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NheI G'CTAG_C Cuts at: 1052 2303 1052 Size: 1251 5374 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 3334 3983 5528 1132 Size: 97 2105 649 1545 2229 Fragments arranged by size: 2229 2105 1545 649 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 5524 5524 Size: 6625 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 1108 Size: 1380 5245 PfoI T'CCnGG_A Cuts at: 2370 2898 2370 Size: 528 6097 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PmlI CAC'GTG Cuts at: 2011 2011 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 2344 2604 3106 2220 Size: 124 260 502 5739 Fragments arranged by size: 5739 502 260 124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PspOMI G'GGCC_C Cuts at: 2053 2138 3273 3809 2053 Size: 85 1135 536 4869 Fragments arranged by size: 4869 1135 536 85 PstI C_TGCA'G Cuts at: 830 2049 2670 3882 3977 830 Size: 1219 621 1212 95 3478 Fragments arranged by size: 3478 1219 1212 621 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SapI GCTCTTCn'nnn_ Cuts at: 2183 2183 Size: 6625 SbfI CC_TGCA'GG Cuts at: 2670 3977 2670 Size: 1307 5318 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SfcI C'TryA_G Cuts at: 826 1045 2045 2666 3076 3124 3293 3878 3973 Size: 219 1000 621 410 48 169 585 95 Cuts at: 3973 5108 5789 5980 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1000 681 621 585 410 219 191 169 95 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2227 4729 5630 5892 820 Size: 197 926 284 2502 901 262 1553 Fragments arranged by size: 2502 1553 926 901 284 262 197 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 3158 3401 1517 Size: 1641 243 4741 Fragments arranged by size: 4741 1641 243 StyI C'CwwG_G Cuts at: 513 1065 2271 2487 3378 3675 4064 513 Size: 552 1206 216 891 297 389 3074 Fragments arranged by size: 3074 1206 891 552 389 297 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 3371 3560 4652 4669 4822 5007 Size: 1255 543 1248 189 1092 17 153 185 Cuts at: 5007 5452 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1248 1092 543 445 189 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3360 4871 6583 Size: 274 80 33 51 1443 1383 1511 1712 Cuts at: 6583 96 Size: 138 Fragments arranged by size: 1712 1511 1443 1383 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 6585 6617 2439 Size: 32 4114 32 2447 Fragments arranged by size: 4114 2447 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2921 1316 Size: 1605 5020 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3664 3764 3664 Size: 100 6525 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 4754 4754 Size: 6625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3030 3892 4433 276 Size: 53 83 186 936 1496 862 541 2468 Fragments arranged by size: 2468 1496 936 862 541 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI