Restriction map for
pFN21AE5729
(Linear) (Six-base) MAPSORT of: KIEE5729.seq Check: 8894 from: 1 to: 1920
KIEE5729.orf
With 182 enzymes: SgfI *
December 5, 2014 13:08 ..
AatII G_ACGT'C
Cuts at: 0 1027 1889 1920
Size: 1027 862 31
Fragments arranged by size:
1027 862 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 355 904 1036 1920
Size: 43 312 549 132 884
Fragments arranged by size:
884 549 312 132 43
AflIII A'CryG_T
Cuts at: 0 59 1920
Size: 59 1861
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 769 801 1920
Size: 769 32 1119
Fragments arranged by size:
1119 769 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1827 1881 1920
Size: 1827 54 39
Fragments arranged by size:
1827 54 39
ApaI G_GGCC'C
Cuts at: 0 52 137 1272 1808 1920
Size: 52 85 1135 536 112
Fragments arranged by size:
1135 536 112 85 52
ApaLI G'TGCA_C
Cuts at: 0 1080 1920
Size: 1080 840
ApoI r'AATT_y
Cuts at: 0 874 1501 1920
Size: 874 627 419
Fragments arranged by size:
874 627 419
BanI G'GyrC_C
Cuts at: 0 687 1840 1920
Size: 687 1153 80
Fragments arranged by size:
1153 687 80
BanII G_rGCy'C
Cuts at: 0 52 137 764 1070 1272 1597 1701 1808
Size: 52 85 627 306 202 325 104 107
Cuts at: 1808 1920
Size: 112
Fragments arranged by size:
627 325 306 202 112 107 104 85
52
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1150 1920
Size: 917 233 770
Fragments arranged by size:
917 770 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 836 870 1920
Size: 836 34 1050
Fragments arranged by size:
1050 836 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1577 1920
Size: 1577 343
BglII A'GATC_T
Cuts at: 0 545 1284 1920
Size: 545 739 636
Fragments arranged by size:
739 636 545
BlpI GC'TnA_GC
Cuts at: 0 391 531 758 1920
Size: 391 140 227 1162
Fragments arranged by size:
1162 391 227 140
Bme1580I G_kGCm'C
Cuts at: 0 52 137 1084 1272 1808 1920
Size: 52 85 947 188 536 112
Fragments arranged by size:
947 536 188 112 85 52
BmgBI CAC'GTC
Cuts at: 0 785 1419 1920
Size: 785 634 501
Fragments arranged by size:
785 634 501
BmrI ACTGGGnnnn_n'
Cuts at: 0 597 837 1275 1347 1808 1811 1920
Size: 597 240 438 72 461 3 109
Fragments arranged by size:
597 461 438 240 109 72 3
BmtI G_CTAG'C
Cuts at: 0 302 1920
Size: 302 1618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1920
Size: 388 1532
Bpu10I CC'TnA_GC
Cuts at: 0 386 1459 1920
Size: 386 1073 461
Fragments arranged by size:
1073 461 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1920
Size: 243 1677
BsaI GGTCTCn'nnnn_
Cuts at: 0 1306 1920
Size: 1306 614
BsaAI yAC'GTr
Cuts at: 0 6 62 1920
Size: 6 56 1858
Fragments arranged by size:
1858 56 6
BsaBI GATnn'nnATC
Cuts at: 0 1366 1630 1651 1920
Size: 1366 264 21 269
Fragments arranged by size:
1366 269 264 21
BsaHI Gr'CG_yC
Cuts at: 0 1024 1886 1920
Size: 1024 862 34
Fragments arranged by size:
1024 862 34
BsaWI w'CCGG_w
Cuts at: 0 614 773 1920
Size: 614 159 1147
Fragments arranged by size:
1147 614 159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1846 1876 1920
Size: 1846 30 44
Fragments arranged by size:
1846 44 30
BseYI C'CCAG_C
Cuts at: 0 227 1920
Size: 227 1693
BsiEI CG_ry'CG
Cuts at: 0 941 1920
Size: 941 979
BsiHKAI G_wGCw'C
Cuts at: 0 1084 1920
Size: 1084 836
BsmBI CGTCTCn'nnnn_
Cuts at: 0 246 1015 1920
Size: 246 769 905
Fragments arranged by size:
905 769 246
Bsp1286I G_dGCh'C
Cuts at: 0 52 137 764 1070 1084 1272 1597 1701
Size: 52 85 627 306 14 188 325 104
Cuts at: 1701 1808 1920
Size: 107 112
Fragments arranged by size:
627 325 306 188 112 107 104 85
52 14
BspEI T'CCGG_A
Cuts at: 0 614 1920
Size: 614 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 1324 1920
Size: 26 1298 596
Fragments arranged by size:
1298 596 26
BsrBI CCG'CTC
Cuts at: 0 1434 1920
Size: 1434 486
BsrDI GCAATG_nn'
Cuts at: 0 300 1272 1350 1920
Size: 300 972 78 570
Fragments arranged by size:
972 570 300 78
BssSI C'ACGA_G
Cuts at: 0 961 1039 1920
Size: 961 78 881
Fragments arranged by size:
961 881 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 39 1827 1920
Size: 39 1788 93
Fragments arranged by size:
1788 93 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1374 1920
Size: 1374 546
BstYI r'GATC_y
Cuts at: 0 545 1284 1920
Size: 545 739 636
Fragments arranged by size:
739 636 545
BtgI C'CryG_G
Cuts at: 0 105 266 482 558 935 1227 1373 1730
Size: 105 161 216 76 377 292 146 357
Cuts at: 1730 1920
Size: 190
Fragments arranged by size:
377 357 292 216 190 161 146 105
76
BtsI GCAGTG_nn'
Cuts at: 0 202 1219 1920
Size: 202 1017 701
Fragments arranged by size:
1017 701 202
DrdI GACnn_nn'nnGTC
Cuts at: 0 1023 1920
Size: 1023 897
EaeI y'GGCC_r
Cuts at: 0 572 857 938 1203 1569 1673 1733 1920
Size: 572 285 81 265 366 104 60 187
Fragments arranged by size:
572 366 285 265 187 104 81 60
EagI C'GGCC_G
Cuts at: 0 938 1920
Size: 938 982
EarI CTCTTCn'nnn_
Cuts at: 0 178 1920
Size: 178 1742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 355 388 904 1036 1920
Size: 43 312 33 516 132 884
Fragments arranged by size:
884 516 312 132 43 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 1920
Size: 22 1898
EcoO109I rG'GnC_Cy
Cuts at: 0 27 48 49 133 215 339 599 1101
Size: 27 21 1 84 82 124 260 502
Cuts at: 1101 1805 1824 1920
Size: 704 19 96
Fragments arranged by size:
704 502 260 124 96 84 82 27
21 19 1
EcoRI G'AATT_C
Cuts at: 0 874 1920
Size: 874 1046
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 955 987 1920
Size: 955 32 933
Fragments arranged by size:
955 933 32
HindIII A'AGCT_T
Cuts at: 0 1048 1920
Size: 1048 872
MscI TGG'CCA
Cuts at: 0 574 1205 1571 1675 1920
Size: 574 631 366 104 245
Fragments arranged by size:
631 574 366 245 104
MslI CAynn'nnrTG
Cuts at: 0 401 1324 1372 1920
Size: 401 923 48 548
Fragments arranged by size:
923 548 401 48
MspA1I CmG'CkG
Cuts at: 0 1692 1881 1920
Size: 1692 189 39
Fragments arranged by size:
1692 189 39
NcoI C'CATG_G
Cuts at: 0 266 482 1373 1920
Size: 266 216 891 547
Fragments arranged by size:
891 547 266 216
NheI G'CTAG_C
Cuts at: 0 298 1920
Size: 298 1622
NspI r_CATG'y
Cuts at: 0 1329 1920
Size: 1329 591
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1920
Size: 483 1437
PfoI T'CCnGG_A
Cuts at: 0 365 893 1920
Size: 365 528 1027
Fragments arranged by size:
1027 528 365
PmlI CAC'GTG
Cuts at: 0 6 1920
Size: 6 1914
PpuMI rG'GwC_Cy
Cuts at: 0 215 339 599 1101 1920
Size: 215 124 260 502 819
Fragments arranged by size:
819 502 260 215 124
PspOMI G'GGCC_C
Cuts at: 0 48 133 1268 1804 1920
Size: 48 85 1135 536 116
Fragments arranged by size:
1135 536 116 85 48
PstI C_TGCA'G
Cuts at: 0 44 665 1877 1920
Size: 44 621 1212 43
Fragments arranged by size:
1212 621 44 43
SapI GCTCTTCn'nnn_
Cuts at: 0 178 1920
Size: 178 1742
SbfI CC_TGCA'GG
Cuts at: 0 665 1920
Size: 665 1255
SfcI C'TryA_G
Cuts at: 0 40 661 1071 1119 1288 1873 1920
Size: 40 621 410 48 169 585 47
Fragments arranged by size:
621 585 410 169 48 47 40
SmlI C'TyrA_G
Cuts at: 0 222 1920
Size: 222 1698
StuI AGG'CCT
Cuts at: 0 1153 1396 1920
Size: 1153 243 524
Fragments arranged by size:
1153 524 243
StyI C'CwwG_G
Cuts at: 0 266 482 1373 1670 1920
Size: 266 216 891 297 250
Fragments arranged by size:
891 297 266 250 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 1366 1555 1920
Size: 118 1248 189 365
Fragments arranged by size:
1248 365 189 118
TatI w'GTAC_w
Cuts at: 0 1355 1920
Size: 1355 565
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 1920
Size: 434 32 1454
Fragments arranged by size:
1454 434 32
Tth111I GACn'n_nGTC
Cuts at: 0 916 1920
Size: 916 1004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1659 1759 1920
Size: 1659 100 161
Fragments arranged by size:
1659 161 100
ZraI GAC'GTC
Cuts at: 0 1025 1887 1920
Size: 1025 862 33
Fragments arranged by size:
1025 862 33
Enzymes that do cut:
AatII AcuI AflIII AloI AlwNI ApaI ApaLI ApoI
BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstXI
BstYI BtgI BtsI DrdI EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI Hin4I HindIII MscI MslI MspA1I
NcoI NheI NspI PflMI PfoI PmlI PpuMI PspOMI
PstI SapI SbfI SfcI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BfrBI BplI BseRI
BsgI BsiWI BsmI BspHI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmaI XmnI