################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII AvrII BfrI BlnI BlpI Bpu1102I BsiEI BsiWI Bsp1407I BspDI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SfiI SfuI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 2795 GGTACC G^GTACC Alw44I 1228 GTGCAC G^TGCAC Aor51HI 3072 AGCGCT AGC^GCT ApaI 2498 GGGCCC GGGCC^C ApaLI 1228 GTGCAC G^TGCAC Asp700I 1776 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2795 GGTACC G^GTACC AspI 883 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3003 GGATCC G^GATCC BbeI 3271 GGCGCC GGCGC^C BbrPI 184 CACGTG CAC^GTG BcgI 2019 CGACTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 184 CACGTG [CT]AC^GT[GA] BsaBI 91 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 147 GAATGC GAATGC(1/-1) BspLU11I 2958 ACATGT A^CATGT BsrBI 592 GAGCGG CCGCTC(-3/-3) BstEII 3204 GGTGACC G^GT[ACGT]ACC BstPI 3204 GGTGACC G^GT[ACGT]ACC DraIII 3009 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 3072 AGCGCT AGC^GCT EcoO65I 3204 GGTGACC G^GT[ACGT]ACC KasI 3271 GGCGCC G^GCGCC KpnI 2795 GGTACC GGTAC^C MaeII 185 ACGT A^CGT MamI 91 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 3271 GGCGCC GG^CGCC NheI 3304 GCTAGC G^CTAGC PflFI 883 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 184 CACGTG CAC^GTG PmlI 184 CACGTG CAC^GTG PshAI 1983 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 261 CCTGCAGG CCTGCA^GG ScaI 2259 AGTACT AGT^ACT SexAI 1253 ACCAGGT A^CC[AT]GGT SfoI 3271 GGCGCC GGC^GCC SgrAI 3220 CGCCGGCG C[GA]^CCGG[CT]G SmaI 2766 CCCGGG CCC^GGG Sse8387I 261 CCTGCAGG CCTGCA^GG Tth111I 883 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2766 CCCGGG C^CCGGG XmaI 2766 CCCGGG C^CCGGG XmnI 1776 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccIII 3077 TCCGGA T^CCGGA 3186 TCCGGA AflIII 185 ACGTGT A^C[GA][CT]GT 2958 ACATGT BanI 2795 GGTACC G^G[CT][GA]CC 3271 GGCGCC BclI 1058 TGATCA T^GATCA 1928 TGATCA BglII 651 AGATCT A^GATCT 2903 AGATCT BsaWI 3077 TCCGGA [AT]^CCGG[AT] 3186 TCCGGA BseAI 3077 TCCGGA T^CCGGA 3186 TCCGGA BseRI 939 CTCCTC GAGGAG(10/8) 1675 GAGGAG BsmBI 1431 CGTCTC CGTCTC(1/5) 2658 CGTCTC BspEI 3077 TCCGGA T^CCGGA 3186 TCCGGA BstAPI 87 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 291 GCAGCGTCTGC FbaI 1058 TGATCA T^GATCA 1928 TGATCA KspI 3161 CCGCGG CCGC^GG 3197 CCGCGG MroI 3077 TCCGGA T^CCGGA 3186 TCCGGA NspI 2627 ACATGC [GA]CATG^[CT] 2958 ACATGT SacI 686 GAGCTC GAGCT^C 3350 GAGCTC SacII 3161 CCGCGG CCGC^GG 3197 CCGCGG SapI 1941 GAAGAGC GCTCTTC(1/4) 2832 GAAGAGC SmlI 1037 CTCAAG C^T[CT][GA]AG 2185 CTCAAG StuI 1379 AGGCCT AGG^CCT 1669 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII