(Circular) (Six-base) MAPSORT of: pFN21AE2743.seq Check: 9037 from: 1 to: 8122 pFN21AE2743 8122 bp With 182 enzymes: SgfI * June 21, 2019 12:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3472 3472 Size: 8122 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5932 278 Size: 53 83 186 936 4396 2468 Fragments arranged by size: 4396 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5465 1937 Size: 3528 4594 Acc65I G'GTAC_C Cuts at: 4800 5443 4800 Size: 643 7479 AclI AA'CG_TT Cuts at: 1563 6249 6622 1563 Size: 4686 373 3063 Fragments arranged by size: 4686 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 3975 4625 4856 5013 6185 7569 7837 Size: 1994 349 650 231 157 1172 1384 268 Cuts at: 7837 1632 Size: 1917 Fragments arranged by size: 1994 1917 1384 1172 650 349 268 231 157 AfeI AGC'GCT Cuts at: 5079 5079 Size: 8122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7925 AflIII A'CryG_T Cuts at: 1203 2190 4963 7021 7998 1203 Size: 987 2773 2058 977 1327 Fragments arranged by size: 2773 2058 1327 987 977 AgeI A'CCGG_T Cuts at: 6953 6953 Size: 8122 AhdI GACnn_n'nnGTC Cuts at: 6851 6851 Size: 8122 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4194 5149 1184 Size: 3010 955 4157 Fragments arranged by size: 4157 3010 955 AlwNI CAG_nnn'CTG Cuts at: 2302 2320 2515 3424 3637 4018 4149 4834 5865 Size: 18 195 909 213 381 131 685 1031 Cuts at: 5865 7437 2302 Size: 1572 2987 Fragments arranged by size: 2987 1572 1031 909 685 381 213 195 131 18 ApaI G_GGCC'C Cuts at: 4507 4507 Size: 8122 ApaLI G'TGCA_C Cuts at: 3233 6179 7335 3233 Size: 2946 1156 4020 Fragments arranged by size: 4020 2946 1156 ApoI r'AATT_y Cuts at: 1493 2083 2233 4364 4916 5431 5657 5809 7918 Size: 590 150 2131 552 515 226 152 2109 Cuts at: 7918 1493 Size: 1697 Fragments arranged by size: 2131 2109 1697 590 552 515 226 152 150 AseI AT'TA_AT Cuts at: 160 1032 6676 160 Size: 872 5644 1606 Fragments arranged by size: 5644 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8122 AvaI C'yCGr_G Cuts at: 1870 1943 2607 3224 4771 5447 1870 Size: 73 664 617 1547 676 4545 Fragments arranged by size: 4545 1547 676 664 617 73 BamHI G'GATC_C Cuts at: 5008 5452 5819 5008 Size: 444 367 7311 Fragments arranged by size: 7311 444 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4800 5276 5443 6898 618 Size: 325 217 627 3013 476 167 1455 1842 Fragments arranged by size: 3013 1842 1455 627 476 325 217 167 BanII G_rGCy'C Cuts at: 1391 2462 2695 4507 5359 5441 1391 Size: 1071 233 1812 852 82 4072 Fragments arranged by size: 4072 1812 1071 852 233 82 BbeI G_GCGC'C Cuts at: 5280 5280 Size: 8122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 2391 3513 4539 928 Size: 971 446 46 1122 1026 4511 Fragments arranged by size: 4511 1122 1026 971 446 46 BbvCI CC'TCA_GC Cuts at: 2555 3485 4218 4590 4721 2555 Size: 930 733 372 131 5956 Fragments arranged by size: 5956 930 733 372 131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4013 4047 6313 6347 1903 Size: 34 2076 34 2266 34 3678 Fragments arranged by size: 3678 2266 2076 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3460 3491 4811 4835 6014 7224 3460 Size: 31 1320 24 1179 1210 4358 Fragments arranged by size: 4358 1320 1210 1179 31 24 BclI T'GATC_A Cuts at: 1548 3063 3933 1548 Size: 1515 870 5737 Fragments arranged by size: 5737 1515 870 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3038 3354 4502 Size: 107 122 71 631 605 1366 316 1148 Cuts at: 4502 6733 136 Size: 2231 1525 Fragments arranged by size: 2231 1525 1366 1148 631 605 316 122 107 71 BglII A'GATC_T Cuts at: 2656 4908 8117 2656 Size: 2252 3209 2661 Fragments arranged by size: 3209 2661 2252 BlpI GC'TnA_GC Cuts at: 5539 5539 Size: 8122 Bme1580I G_kGCm'C Cuts at: 1790 3237 3927 4507 6183 7339 1790 Size: 1447 690 580 1676 1156 2573 Fragments arranged by size: 2573 1676 1447 1156 690 580 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3436 3844 3910 Size: 416 53 83 399 24 2019 408 66 Cuts at: 3910 5853 6811 442 Size: 1943 958 1753 Fragments arranged by size: 2019 1943 1753 958 416 408 399 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 5313 1056 Size: 4257 3865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3651 3651 Size: 8122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2345 2516 2546 2564 2951 4529 4666 Size: 1073 33 171 30 18 387 1578 137 Cuts at: 4666 4727 5243 5345 6782 1239 Size: 61 516 102 1437 2579 Fragments arranged by size: 2579 1578 1437 1073 516 387 171 137 102 61 33 30 18 Bpu10I CC'TnA_GC Cuts at: 2555 2910 3485 4167 4218 4590 4721 4748 2555 Size: 355 575 682 51 372 131 27 5929 Fragments arranged by size: 5929 682 575 372 355 131 51 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3027 4175 6247 7112 7410 3027 Size: 1148 2072 865 298 3739 Fragments arranged by size: 3739 2072 1148 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3152 3268 4229 6785 882 Size: 617 1653 116 961 2556 2219 Fragments arranged by size: 2556 2219 1653 961 617 116 BsaAI yAC'GTr Cuts at: 493 1204 2191 5913 493 Size: 711 987 3722 2702 Fragments arranged by size: 3722 2702 987 711 BsaBI GATnn'nnATC Cuts at: 2100 8116 2100 Size: 6016 2106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2346 3099 5277 5929 Size: 53 83 186 936 813 753 2178 652 Cuts at: 5929 6311 6946 275 Size: 382 635 1451 Fragments arranged by size: 2178 1451 936 813 753 652 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5082 5191 6555 6953 7227 7374 7785 5082 Size: 109 1364 398 274 147 411 5419 Fragments arranged by size: 5419 1364 411 398 274 147 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4709 4739 1178 Size: 30 165 30 3306 30 4561 Fragments arranged by size: 4561 3306 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2935 3695 1186 Size: 1749 760 5613 Fragments arranged by size: 5613 1749 760 BseYI C'CCAG_C Cuts at: 2261 2349 2986 3368 3468 3695 3907 4415 4500 Size: 88 637 382 100 227 212 508 85 Cuts at: 4500 4561 5220 7325 7821 2261 Size: 61 659 2105 496 2562 Fragments arranged by size: 2562 2105 659 637 508 496 382 227 212 100 88 85 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2288 3091 3861 4028 1163 Size: 573 552 803 770 167 5257 Fragments arranged by size: 5257 803 770 573 552 167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5572 6333 7361 1540 Size: 275 186 3571 761 1028 2301 Fragments arranged by size: 3571 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2695 3237 3746 5359 6183 6268 7339 2695 Size: 542 509 1613 824 85 1071 3478 Fragments arranged by size: 3478 1613 1071 824 542 509 85 BsmI GAATG_Cn' Cuts at: 2158 5633 5726 2158 Size: 3475 93 4554 Fragments arranged by size: 4554 3475 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3442 4669 3442 Size: 1227 6895 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2462 2695 3237 3746 3927 4507 5359 Size: 399 672 233 542 509 181 580 852 Cuts at: 5359 5441 6183 6268 7339 1391 Size: 82 742 85 1071 2174 Fragments arranged by size: 2174 1071 852 742 672 580 542 509 399 233 181 85 82 BspEI T'CCGG_A Cuts at: 5082 5191 7785 5082 Size: 109 2594 5419 Fragments arranged by size: 5419 2594 109 BspHI T'CATG_A Cuts at: 6011 7741 6011 Size: 1730 6392 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2622 3409 3472 3728 4074 4524 5477 844 Size: 1778 787 63 256 346 450 953 3489 Fragments arranged by size: 3489 1778 953 787 450 346 256 63 BsrBI CCG'CTC Cuts at: 1394 1823 2599 6009 1394 Size: 429 776 3410 3507 Fragments arranged by size: 3507 3410 776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3129 4029 4677 5052 6617 6791 65 Size: 1183 1881 900 648 375 1565 174 1396 Fragments arranged by size: 1881 1565 1396 1183 900 648 375 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4081 4584 5226 5315 6766 6953 1700 Size: 110 2271 503 642 89 1451 187 2869 Fragments arranged by size: 2869 2271 1451 642 503 187 110 89 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8122 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8122 BssSI C'ACGA_G Cuts at: 6182 7194 6182 Size: 1012 7110 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 2302 794 Size: 1304 204 6614 Fragments arranged by size: 6614 1304 204 BstBI TT'CG_AA Cuts at: 6926 6926 Size: 8122 BstEII G'GTnAC_C Cuts at: 1184 5209 1184 Size: 4025 4097 BstXI CCAn_nnnn'nTGG Cuts at: 2884 3310 4644 2884 Size: 426 1334 6362 Fragments arranged by size: 6362 1334 426 BstYI r'GATC_y Cuts at: 1972 2164 2538 2656 4104 4314 4410 4908 5008 Size: 192 374 118 1448 210 96 498 100 Cuts at: 5008 5452 5819 6204 6221 7673 7759 7771 8117 Size: 444 367 385 17 1452 86 12 346 Cuts at: 8117 1972 Size: 1977 Fragments arranged by size: 1977 1452 1448 498 444 385 374 367 346 210 192 118 100 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2333 2566 3080 3678 3998 2333 Size: 233 514 598 320 6457 Fragments arranged by size: 6457 598 514 320 233 BtgI C'CryG_G Cuts at: 513 1065 2056 2277 3135 4441 4551 5166 5202 Size: 552 991 221 858 1306 110 615 36 Cuts at: 5202 513 Size: 3433 Fragments arranged by size: 3433 1306 991 858 615 552 221 110 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7383 Fragments arranged by size: 7383 683 56 BtsI GCAGTG_nn' Cuts at: 2156 2361 2652 3359 3510 4019 4188 4408 5240 Size: 205 291 707 151 509 169 220 832 Cuts at: 5240 5638 6432 6452 2156 Size: 398 794 20 3826 Fragments arranged by size: 3826 832 794 707 509 398 291 220 205 169 151 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8122 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8087 DraI TTT'AAA Cuts at: 5426 5426 Size: 8122 DraIII CAC_nnn'GTG Cuts at: 5019 5019 Size: 8122 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7129 809 Size: 507 5813 1802 Fragments arranged by size: 5813 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4341 4482 4932 Size: 54 1425 50 275 18 2511 141 450 Cuts at: 4932 5406 5569 6458 8087 8 Size: 474 163 889 1629 43 Fragments arranged by size: 2511 1629 1425 889 474 450 275 163 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5569 1537 Size: 275 3757 4090 Fragments arranged by size: 4090 3757 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3941 4465 4832 6052 1353 Size: 2588 524 367 1220 3423 Fragments arranged by size: 3423 2588 1220 524 367 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2138 6709 7083 7229 387 Size: 1064 138 549 4571 374 146 1280 Fragments arranged by size: 4571 1280 1064 549 374 146 138 EcoICRI GAG'CTC Cuts at: 2693 5357 2693 Size: 2664 5458 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2345 2516 2546 2564 2951 3626 Size: 393 680 33 171 30 18 387 675 Cuts at: 3626 3975 4529 4625 4666 4727 4856 5013 5243 Size: 349 554 96 41 61 129 157 230 Cuts at: 5243 5345 6185 6782 7569 7837 1239 Size: 102 840 597 787 268 1524 Fragments arranged by size: 1524 840 787 680 675 597 554 393 387 349 268 230 171 157 129 102 96 61 41 33 30 18 EcoNI CCTnn'n_nnAGG Cuts at: 2546 2618 3257 5261 2546 Size: 72 639 2004 5407 Fragments arranged by size: 5407 2004 639 72 EcoO109I rG'GnC_Cy Cuts at: 2329 2562 2611 3421 4253 4347 4504 4513 4831 Size: 233 49 810 832 94 157 9 318 Cuts at: 4831 4944 2329 Size: 113 5507 Fragments arranged by size: 5507 832 810 318 233 157 113 94 49 9 EcoRI G'AATT_C Cuts at: 2233 4364 4916 5431 5809 2233 Size: 2131 552 515 378 4546 Fragments arranged by size: 4546 2131 552 515 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 3068 2416 Size: 652 7470 FspI TGC'GCA Cuts at: 6628 6628 Size: 8122 HaeII r_GCGC'y Cuts at: 1678 5081 5227 5270 5280 5316 7269 1678 Size: 3403 146 43 10 36 1953 2531 Fragments arranged by size: 3403 2531 1953 146 43 36 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2657 2689 4622 Size: 32 135 32 620 32 433 32 1933 Cuts at: 4622 4654 4709 4741 4809 4841 6763 6795 6837 Size: 32 55 32 68 32 1922 32 42 Cuts at: 6837 6869 1373 Size: 32 2626 Fragments arranged by size: 2626 1933 1922 620 433 135 68 55 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5466 5712 669 Size: 988 281 3528 246 3079 Fragments arranged by size: 3528 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8122 HpaI GTT'AAC Cuts at: 5712 5712 Size: 8122 KasI G'GCGC_C Cuts at: 5276 5276 Size: 8122 KpnI G_GTAC'C Cuts at: 4804 5447 4804 Size: 643 7479 MfeI C'AATT_G Cuts at: 5721 5721 Size: 8122 MluI A'CGCG_T Cuts at: 7998 7998 Size: 8122 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 3156 3477 3936 7236 7420 1305 Size: 1668 183 321 459 3300 184 2007 Fragments arranged by size: 3300 2007 1668 459 321 184 183 MscI TGG'CCA Cuts at: 10 64 1489 1832 4343 4934 5408 10 Size: 54 1425 343 2511 591 474 2724 Fragments arranged by size: 2724 2511 1425 591 474 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2366 3134 3732 6080 6439 6598 518 Size: 648 1200 768 598 2348 359 159 2042 Fragments arranged by size: 2348 2042 1200 768 648 598 359 159 MspA1I CmG'CkG Cuts at: 1606 2884 3703 4594 4714 5060 5168 5204 5248 Size: 1278 819 891 120 346 108 36 44 Cuts at: 5248 5538 6215 7363 7608 1606 Size: 290 677 1148 245 2120 Fragments arranged by size: 2120 1278 1148 891 819 677 346 290 245 120 108 44 36 NaeI GCC'GGC Cuts at: 4083 4586 5228 4083 Size: 503 642 6977 Fragments arranged by size: 6977 642 503 NarI GG'CG_CC Cuts at: 5277 5277 Size: 8122 NcoI C'CATG_G Cuts at: 513 1065 2056 3135 4441 513 Size: 552 991 1079 1306 4194 Fragments arranged by size: 4194 1306 1079 991 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8122 NgoMIV G'CCGG_C Cuts at: 4081 4584 5226 4081 Size: 503 642 6977 Fragments arranged by size: 6977 642 503 NheI G'CTAG_C Cuts at: 1052 5309 1052 Size: 4257 3865 NotI GC'GGCC_GC Cuts at: 5569 5569 Size: 8122 NspI r_CATG'y Cuts at: 1132 1229 4636 4967 5480 7025 1132 Size: 97 3407 331 513 1545 2229 Fragments arranged by size: 3407 2229 1545 513 331 97 PasI CC'CwG_GG Cuts at: 1348 3163 4113 4669 5185 1348 Size: 1815 950 556 516 4285 Fragments arranged by size: 4285 1815 950 556 516 PciI A'CATG_T Cuts at: 4963 7021 4963 Size: 2058 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 4119 5019 1108 Size: 1878 1133 900 4211 Fragments arranged by size: 4211 1878 1133 900 PfoI T'CCnGG_A Cuts at: 2237 2702 3123 3768 4827 2237 Size: 465 421 645 1059 5532 Fragments arranged by size: 5532 1059 645 465 421 PmeI GTTT'AAAC Cuts at: 5426 5426 Size: 8122 PmlI CAC'GTG Cuts at: 2191 2191 Size: 8122 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6191 6223 7704 7736 6191 Size: 32 1481 32 6577 Fragments arranged by size: 6577 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2329 2562 2611 3421 4253 4347 4513 4831 4944 Size: 233 49 810 832 94 166 318 113 Cuts at: 4944 2329 Size: 5507 Fragments arranged by size: 5507 832 810 318 233 166 113 94 49 PshAI GACnn'nnGTC Cuts at: 1613 3992 1613 Size: 2379 5743 PsiI TTA'TAA Cuts at: 4904 5692 4904 Size: 788 7334 PspOMI G'GGCC_C Cuts at: 4503 4503 Size: 8122 PstI C_TGCA'G Cuts at: 830 2271 2647 2724 3395 4183 4958 5247 5253 Size: 1441 376 77 671 788 775 289 6 Cuts at: 5253 5474 830 Size: 221 3478 Fragments arranged by size: 3478 1441 788 775 671 376 289 221 77 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8122 PvuII CAG'CTG Cuts at: 2884 3703 4594 4714 5060 5248 2884 Size: 819 891 120 346 188 5758 Fragments arranged by size: 5758 891 819 346 188 120 SacI G_AGCT'C Cuts at: 2695 5359 2695 Size: 2664 5458 SacII CC_GC'GG Cuts at: 5169 5205 5169 Size: 36 8086 SalI G'TCGA_C Cuts at: 1936 5464 1936 Size: 3528 4594 SanDI GG'GwC_CC Cuts at: 4253 4944 4253 Size: 691 7431 SapI GCTCTTCn'nnn_ Cuts at: 3941 4832 3941 Size: 891 7231 SbfI CC_TGCA'GG Cuts at: 2271 5474 2271 Size: 3203 4919 ScaI AGT'ACT Cuts at: 4266 6370 8082 4266 Size: 2104 1712 4306 Fragments arranged by size: 4306 2104 1712 SexAI A'CCwGG_T Cuts at: 3258 3258 Size: 8122 SfcI C'TryA_G Cuts at: 826 1045 2267 2643 2720 2838 3199 3391 3597 Size: 219 1222 376 77 118 361 192 206 Cuts at: 3597 4179 4293 4954 5243 5249 5470 6605 7286 Size: 582 114 661 289 6 221 1135 681 Cuts at: 7286 7477 826 Size: 191 1471 Fragments arranged by size: 1471 1222 1135 681 661 582 376 361 289 221 219 206 192 191 118 114 77 6 SfoI GGC'GCC Cuts at: 5278 5278 Size: 8122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8122 SgrAI Cr'CCGG_yG Cuts at: 1700 5226 1700 Size: 3526 4596 SmaI CCC'GGG Cuts at: 1872 4773 5449 1872 Size: 2901 676 4545 Fragments arranged by size: 4545 2901 676 SmlI C'TyrA_G Cuts at: 820 1017 1943 3042 4190 6226 7127 7389 820 Size: 197 926 1099 1148 2036 901 262 1553 Fragments arranged by size: 2036 1553 1148 1099 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5913 493 Size: 5420 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8122 SphI G_CATG'C Cuts at: 1132 5480 1132 Size: 4348 3774 SspI AAT'ATT Cuts at: 5 52 6046 5 Size: 47 5994 2081 Fragments arranged by size: 5994 2081 47 StuI AGG'CCT Cuts at: 1517 3386 3676 1517 Size: 1869 290 5963 Fragments arranged by size: 5963 1869 290 StyI C'CwwG_G Cuts at: 513 1065 2056 3135 3963 4441 5561 513 Size: 552 991 1079 828 478 1120 3074 Fragments arranged by size: 3074 1120 1079 991 828 552 478 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3395 6149 6166 6319 6504 6949 325 Size: 1255 1815 2754 17 153 185 445 1498 Fragments arranged by size: 2754 1815 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4041 4264 6368 Size: 274 80 33 51 1443 2064 223 2104 Cuts at: 6368 8080 96 Size: 1712 138 Fragments arranged by size: 2104 2064 1712 1443 274 223 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4291 4323 4387 4419 4505 4537 8082 8114 4291 Size: 32 64 32 86 32 3545 32 4299 Fragments arranged by size: 4299 3545 86 64 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2891 1316 Size: 1575 6547 XbaI T'CTAG_A Cuts at: 5458 5458 Size: 8122 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 2983 4246 4735 2881 Size: 102 1263 489 6268 Fragments arranged by size: 6268 1263 489 102 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8122 XmaI C'CCGG_G Cuts at: 1870 4771 5447 1870 Size: 2901 676 4545 Fragments arranged by size: 4545 2901 676 XmnI GAAnn'nnTTC Cuts at: 3785 6251 3785 Size: 2466 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5930 276 Size: 53 83 186 936 4396 2468 Fragments arranged by size: 4396 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SfiI SrfI SwaI