Restriction map for
pFN21AE2743
(Linear) (Six-base) MAPSORT of: KIEE2743.seq Check: 8584 from: 1 to: 3417
KIEE2743.orf
With 182 enzymes: SgfI *
June 21, 2019 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1467 3417
Size: 1467 1950
Acc65I G'GTAC_C
Cuts at: 0 2795 3417
Size: 2795 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 1970 2620 2851 3008 3417
Size: 1621 349 650 231 157 409
Fragments arranged by size:
1621 650 409 349 231 157
AfeI AGC'GCT
Cuts at: 0 3074 3417
Size: 3074 343
AflIII A'CryG_T
Cuts at: 0 185 2958 3417
Size: 185 2773 459
Fragments arranged by size:
2773 459 185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2189 3144 3417
Size: 2189 955 273
Fragments arranged by size:
2189 955 273
AlwNI CAG_nnn'CTG
Cuts at: 0 297 315 510 1419 1632 2013 2144 2829
Size: 297 18 195 909 213 381 131 685
Cuts at: 2829 3417
Size: 588
Fragments arranged by size:
909 685 588 381 297 213 195 131
18
ApaI G_GGCC'C
Cuts at: 0 2502 3417
Size: 2502 915
ApaLI G'TGCA_C
Cuts at: 0 1228 3417
Size: 1228 2189
ApoI r'AATT_y
Cuts at: 0 78 228 2359 2911 3417
Size: 78 150 2131 552 506
Fragments arranged by size:
2131 552 506 150 78
AvaI C'yCGr_G
Cuts at: 0 602 1219 2766 3417
Size: 602 617 1547 651
Fragments arranged by size:
1547 651 617 602
BamHI G'GATC_C
Cuts at: 0 3003 3417
Size: 3003 414
BanI G'GyrC_C
Cuts at: 0 2795 3271 3417
Size: 2795 476 146
Fragments arranged by size:
2795 476 146
BanII G_rGCy'C
Cuts at: 0 457 690 2502 3354 3417
Size: 457 233 1812 852 63
Fragments arranged by size:
1812 852 457 233 63
BbeI G_GCGC'C
Cuts at: 0 3275 3417
Size: 3275 142
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 386 1508 2534 3417
Size: 340 46 1122 1026 883
Fragments arranged by size:
1122 1026 883 340 46
BbvCI CC'TCA_GC
Cuts at: 0 550 1480 2213 2585 2716 3417
Size: 550 930 733 372 131 701
Fragments arranged by size:
930 733 701 550 372 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2008 2042 3417
Size: 2008 34 1375
Fragments arranged by size:
2008 1375 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1455 1486 2806 2830 3417
Size: 1455 31 1320 24 587
Fragments arranged by size:
1455 1320 587 31 24
BclI T'GATC_A
Cuts at: 0 1058 1928 3417
Size: 1058 870 1489
Fragments arranged by size:
1489 1058 870
BglI GCCn_nnn'nGGC
Cuts at: 0 1033 1349 2497 3417
Size: 1033 316 1148 920
Fragments arranged by size:
1148 1033 920 316
BglII A'GATC_T
Cuts at: 0 651 2903 3417
Size: 651 2252 514
Fragments arranged by size:
2252 651 514
Bme1580I G_kGCm'C
Cuts at: 0 1232 1922 2502 3417
Size: 1232 690 580 915
Fragments arranged by size:
1232 915 690 580
BmrI ACTGGGnnnn_n'
Cuts at: 0 1431 1839 1905 3417
Size: 1431 408 66 1512
Fragments arranged by size:
1512 1431 408 66
BmtI G_CTAG'C
Cuts at: 0 3308 3417
Size: 3308 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1646 3417
Size: 1646 1771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 340 511 541 559 946 2524 2661
Size: 307 33 171 30 18 387 1578 137
Cuts at: 2661 2722 3238 3340 3417
Size: 61 516 102 77
Fragments arranged by size:
1578 516 387 307 171 137 102 77
61 33 30 18
Bpu10I CC'TnA_GC
Cuts at: 0 550 905 1480 2162 2213 2585 2716 2743
Size: 550 355 575 682 51 372 131 27
Cuts at: 2743 3417
Size: 674
Fragments arranged by size:
682 674 575 550 372 355 131 51
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 2170 3417
Size: 1022 1148 1247
Fragments arranged by size:
1247 1148 1022
BsaI GGTCTCn'nnnn_
Cuts at: 0 1147 1263 2224 3417
Size: 1147 116 961 1193
Fragments arranged by size:
1193 1147 961 116
BsaAI yAC'GTr
Cuts at: 0 186 3417
Size: 186 3231
BsaBI GATnn'nnATC
Cuts at: 0 95 3417
Size: 95 3322
BsaHI Gr'CG_yC
Cuts at: 0 341 1094 3272 3417
Size: 341 753 2178 145
Fragments arranged by size:
2178 753 341 145
BsaWI w'CCGG_w
Cuts at: 0 3077 3186 3417
Size: 3077 109 231
Fragments arranged by size:
3077 231 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2704 2734 3417
Size: 2704 30 683
Fragments arranged by size:
2704 683 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 930 1690 3417
Size: 930 760 1727
Fragments arranged by size:
1727 930 760
BseYI C'CCAG_C
Cuts at: 0 256 344 981 1363 1463 1690 1902 2410
Size: 256 88 637 382 100 227 212 508
Cuts at: 2410 2495 2556 3215 3417
Size: 85 61 659 202
Fragments arranged by size:
659 637 508 382 256 227 212 202
100 88 85 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1086 1856 2023 3417
Size: 283 803 770 167 1394
Fragments arranged by size:
1394 803 770 283 167
BsiHKAI G_wGCw'C
Cuts at: 0 690 1232 1741 3354 3417
Size: 690 542 509 1613 63
Fragments arranged by size:
1613 690 542 509 63
BsmI GAATG_Cn'
Cuts at: 0 153 3417
Size: 153 3264
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1437 2664 3417
Size: 1437 1227 753
Fragments arranged by size:
1437 1227 753
Bsp1286I G_dGCh'C
Cuts at: 0 457 690 1232 1741 1922 2502 3354 3417
Size: 457 233 542 509 181 580 852 63
Fragments arranged by size:
852 580 542 509 457 233 181 63
BspEI T'CCGG_A
Cuts at: 0 3077 3186 3417
Size: 3077 109 231
Fragments arranged by size:
3077 231 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 617 1404 1467 1723 2069 2519 3417
Size: 617 787 63 256 346 450 898
Fragments arranged by size:
898 787 617 450 346 256 63
BsrBI CCG'CTC
Cuts at: 0 594 3417
Size: 594 2823
BsrDI GCAATG_nn'
Cuts at: 0 1124 2024 2672 3047 3417
Size: 1124 900 648 375 370
Fragments arranged by size:
1124 900 648 375 370
BsrFI r'CCGG_y
Cuts at: 0 2076 2579 3221 3310 3417
Size: 2076 503 642 89 107
Fragments arranged by size:
2076 642 503 107 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 297 3417
Size: 93 204 3120
Fragments arranged by size:
3120 204 93
BstEII G'GTnAC_C
Cuts at: 0 3204 3417
Size: 3204 213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 879 1305 2639 3417
Size: 879 426 1334 778
Fragments arranged by size:
1334 879 778 426
BstYI r'GATC_y
Cuts at: 0 159 533 651 2099 2309 2405 2903 3003
Size: 159 374 118 1448 210 96 498 100
Cuts at: 3003 3417
Size: 414
Fragments arranged by size:
1448 498 414 374 210 159 118 100
96
Bsu36I CC'TnA_GG
Cuts at: 0 328 561 1075 1673 1993 3417
Size: 328 233 514 598 320 1424
Fragments arranged by size:
1424 598 514 328 320 233
BtgI C'CryG_G
Cuts at: 0 51 272 1130 2436 2546 3161 3197 3417
Size: 51 221 858 1306 110 615 36 220
Fragments arranged by size:
1306 858 615 221 220 110 51 36
BtsI GCAGTG_nn'
Cuts at: 0 151 356 647 1354 1505 2014 2183 2403
Size: 151 205 291 707 151 509 169 220
Cuts at: 2403 3235 3417
Size: 832 182
Fragments arranged by size:
832 707 509 291 220 205 182 169
151 151
DraIII CAC_nnn'GTG
Cuts at: 0 3014 3417
Size: 3014 403
EaeI y'GGCC_r
Cuts at: 0 2336 2477 2927 3401 3417
Size: 2336 141 450 474 16
Fragments arranged by size:
2336 474 450 141 16
EarI CTCTTCn'nnn_
Cuts at: 0 1936 2460 2827 3417
Size: 1936 524 367 590
Fragments arranged by size:
1936 590 524 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 3417
Size: 133 3284
EcoICRI GAG'CTC
Cuts at: 0 688 3352 3417
Size: 688 2664 65
Fragments arranged by size:
2664 688 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 340 511 541 559 946 1621 1970
Size: 307 33 171 30 18 387 675 349
Cuts at: 1970 2524 2620 2661 2722 2851 3008 3238 3340
Size: 554 96 41 61 129 157 230 102
Cuts at: 3340 3417
Size: 77
Fragments arranged by size:
675 554 387 349 307 230 171 157
129 102 96 77 61 41 33 30
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 541 613 1252 3256 3417
Size: 541 72 639 2004 161
Fragments arranged by size:
2004 639 541 161 72
EcoO109I rG'GnC_Cy
Cuts at: 0 324 557 606 1416 2248 2342 2499 2508
Size: 324 233 49 810 832 94 157 9
Cuts at: 2508 2826 2939 3417
Size: 318 113 478
Fragments arranged by size:
832 810 478 324 318 233 157 113
94 49 9
EcoRI G'AATT_C
Cuts at: 0 228 2359 2911 3417
Size: 228 2131 552 506
Fragments arranged by size:
2131 552 506 228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 411 1063 3417
Size: 411 652 2354
Fragments arranged by size:
2354 652 411
HaeII r_GCGC'y
Cuts at: 0 3076 3222 3265 3275 3311 3417
Size: 3076 146 43 10 36 106
Fragments arranged by size:
3076 146 106 43 36 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 652 684 2617 2649 2704 2736
Size: 187 32 433 32 1933 32 55 32
Cuts at: 2736 2804 2836 3417
Size: 68 32 581
Fragments arranged by size:
1933 581 433 187 68 55 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 3271 3417
Size: 3271 146
KpnI G_GTAC'C
Cuts at: 0 2799 3417
Size: 2799 618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1151 1472 1931 3417
Size: 968 183 321 459 1486
Fragments arranged by size:
1486 968 459 321 183
MscI TGG'CCA
Cuts at: 0 2338 2929 3403 3417
Size: 2338 591 474 14
Fragments arranged by size:
2338 591 474 14
MslI CAynn'nnrTG
Cuts at: 0 361 1129 1727 3417
Size: 361 768 598 1690
Fragments arranged by size:
1690 768 598 361
MspA1I CmG'CkG
Cuts at: 0 879 1698 2589 2709 3055 3163 3199 3243
Size: 879 819 891 120 346 108 36 44
Cuts at: 3243 3417
Size: 174
Fragments arranged by size:
891 879 819 346 174 120 108 44
36
NaeI GCC'GGC
Cuts at: 0 2078 2581 3223 3417
Size: 2078 503 642 194
Fragments arranged by size:
2078 642 503 194
NarI GG'CG_CC
Cuts at: 0 3272 3417
Size: 3272 145
NcoI C'CATG_G
Cuts at: 0 51 1130 2436 3417
Size: 51 1079 1306 981
Fragments arranged by size:
1306 1079 981 51
NgoMIV G'CCGG_C
Cuts at: 0 2076 2579 3221 3417
Size: 2076 503 642 196
Fragments arranged by size:
2076 642 503 196
NheI G'CTAG_C
Cuts at: 0 3304 3417
Size: 3304 113
NspI r_CATG'y
Cuts at: 0 2631 2962 3417
Size: 2631 331 455
Fragments arranged by size:
2631 455 331
PasI CC'CwG_GG
Cuts at: 0 1158 2108 2664 3180 3417
Size: 1158 950 556 516 237
Fragments arranged by size:
1158 950 556 516 237
PciI A'CATG_T
Cuts at: 0 2958 3417
Size: 2958 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 2114 3014 3417
Size: 981 1133 900 403
Fragments arranged by size:
1133 981 900 403
PfoI T'CCnGG_A
Cuts at: 0 232 697 1118 1763 2822 3417
Size: 232 465 421 645 1059 595
Fragments arranged by size:
1059 645 595 465 421 232
PmlI CAC'GTG
Cuts at: 0 186 3417
Size: 186 3231
PpuMI rG'GwC_Cy
Cuts at: 0 324 557 606 1416 2248 2342 2508 2826
Size: 324 233 49 810 832 94 166 318
Cuts at: 2826 2939 3417
Size: 113 478
Fragments arranged by size:
832 810 478 324 318 233 166 113
94 49
PshAI GACnn'nnGTC
Cuts at: 0 1987 3417
Size: 1987 1430
PsiI TTA'TAA
Cuts at: 0 2899 3417
Size: 2899 518
PspOMI G'GGCC_C
Cuts at: 0 2498 3417
Size: 2498 919
PstI C_TGCA'G
Cuts at: 0 266 642 719 1390 2178 2953 3242 3248
Size: 266 376 77 671 788 775 289 6
Cuts at: 3248 3417
Size: 169
Fragments arranged by size:
788 775 671 376 289 266 169 77
6
PvuII CAG'CTG
Cuts at: 0 879 1698 2589 2709 3055 3243 3417
Size: 879 819 891 120 346 188 174
Fragments arranged by size:
891 879 819 346 188 174 120
SacI G_AGCT'C
Cuts at: 0 690 3354 3417
Size: 690 2664 63
Fragments arranged by size:
2664 690 63
SacII CC_GC'GG
Cuts at: 0 3164 3200 3417
Size: 3164 36 217
Fragments arranged by size:
3164 217 36
SanDI GG'GwC_CC
Cuts at: 0 2248 2939 3417
Size: 2248 691 478
Fragments arranged by size:
2248 691 478
SapI GCTCTTCn'nnn_
Cuts at: 0 1936 2827 3417
Size: 1936 891 590
Fragments arranged by size:
1936 891 590
SbfI CC_TGCA'GG
Cuts at: 0 266 3417
Size: 266 3151
ScaI AGT'ACT
Cuts at: 0 2261 3417
Size: 2261 1156
SexAI A'CCwGG_T
Cuts at: 0 1253 3417
Size: 1253 2164
SfcI C'TryA_G
Cuts at: 0 262 638 715 833 1194 1386 1592 2174
Size: 262 376 77 118 361 192 206 582
Cuts at: 2174 2288 2949 3238 3244 3417
Size: 114 661 289 6 173
Fragments arranged by size:
661 582 376 361 289 262 206 192
173 118 114 77 6
SfoI GGC'GCC
Cuts at: 0 3273 3417
Size: 3273 144
SgrAI Cr'CCGG_yG
Cuts at: 0 3221 3417
Size: 3221 196
SmaI CCC'GGG
Cuts at: 0 2768 3417
Size: 2768 649
SmlI C'TyrA_G
Cuts at: 0 1037 2185 3417
Size: 1037 1148 1232
Fragments arranged by size:
1232 1148 1037
StuI AGG'CCT
Cuts at: 0 1381 1671 3417
Size: 1381 290 1746
Fragments arranged by size:
1746 1381 290
StyI C'CwwG_G
Cuts at: 0 51 1130 1958 2436 3417
Size: 51 1079 828 478 981
Fragments arranged by size:
1079 981 828 478 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1390 3417
Size: 1390 2027
TatI w'GTAC_w
Cuts at: 0 2036 2259 3417
Size: 2036 223 1158
Fragments arranged by size:
2036 1158 223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2286 2318 2382 2414 2500 2532 3417
Size: 2286 32 64 32 86 32 885
Fragments arranged by size:
2286 885 86 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 886 3417
Size: 886 2531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 978 2241 2730 3417
Size: 876 102 1263 489 687
Fragments arranged by size:
1263 876 687 489 102
XmaI C'CCGG_G
Cuts at: 0 2766 3417
Size: 2766 651
XmnI GAAnn'nnTTC
Cuts at: 0 1780 3417
Size: 1780 1637
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PstI
PvuII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AloI
AscI AseI AsiSI AvrII BaeI BfrBI BlpI BmgBI
BsiEI BsiWI BspHI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoRV FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NdeI
NotI NruI NsiI PacI PmeI PpiI PspXI PsrI
PvuI RsrII SalI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI ZraI