(Circular) (Six-base) MAPSORT of: pFN21AE3888.seq Check: 273 from: 1 to: 5617 pFN21AE3888 5617 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3427 278 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2960 1937 Size: 1023 4594 Acc65I G'GTAC_C Cuts at: 2125 2938 2125 Size: 813 4804 AclI AA'CG_TT Cuts at: 1563 3744 4117 1563 Size: 2181 373 3063 Fragments arranged by size: 3063 2181 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 3680 5064 5332 1632 Size: 972 1076 1384 268 1917 Fragments arranged by size: 1917 1384 1076 972 268 AfeI AGC'GCT Cuts at: 2342 2342 Size: 5617 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5420 AflIII A'CryG_T Cuts at: 1203 2736 4516 5493 1203 Size: 1533 1780 977 1327 Fragments arranged by size: 1780 1533 1327 977 AgeI A'CCGG_T Cuts at: 2553 4448 2553 Size: 1895 3722 AhdI GACnn_n'nnGTC Cuts at: 4346 4346 Size: 5617 AleI CACnn'nnGTG Cuts at: 1166 2756 2822 1166 Size: 1590 66 3961 Fragments arranged by size: 3961 1590 66 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2437 1184 Size: 1253 4364 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 2270 Size: 32 5585 AlwNI CAG_nnn'CTG Cuts at: 2888 3360 4932 2888 Size: 472 1572 3573 Fragments arranged by size: 3573 1572 472 ApaLI G'TGCA_C Cuts at: 2493 2871 3674 4830 2493 Size: 378 803 1156 3280 Fragments arranged by size: 3280 1156 803 378 ApoI r'AATT_y Cuts at: 1493 2375 2426 2537 2926 3152 3304 5413 1493 Size: 882 51 111 389 226 152 2109 1697 Fragments arranged by size: 2109 1697 882 389 226 152 111 51 AseI AT'TA_AT Cuts at: 160 1032 4171 160 Size: 872 3139 1606 Fragments arranged by size: 3139 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 AvaI C'yCGr_G Cuts at: 1870 1943 2218 2701 2721 2903 2942 1870 Size: 73 275 483 20 182 39 4545 Fragments arranged by size: 4545 483 275 182 73 39 20 BamHI G'GATC_C Cuts at: 2947 3314 2947 Size: 367 5250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2938 4393 618 Size: 325 217 627 338 813 1455 1842 Fragments arranged by size: 1842 1455 813 627 338 325 217 BanII G_rGCy'C Cuts at: 1391 2076 2936 1391 Size: 685 860 4072 Fragments arranged by size: 4072 860 685 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 928 Size: 971 354 4292 Fragments arranged by size: 4292 971 354 BbvCI CC'TCA_GC Cuts at: 2774 2774 Size: 5617 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 1903 Size: 34 1871 34 3678 Fragments arranged by size: 3678 1871 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3509 4719 3509 Size: 1210 4407 BclI T'GATC_A Cuts at: 1548 1548 Size: 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2195 4228 136 Size: 107 122 71 631 605 523 2033 1525 Fragments arranged by size: 2033 1525 631 605 523 122 107 71 BglII A'GATC_T Cuts at: 5612 5612 Size: 5617 BlpI GC'TnA_GC Cuts at: 3034 3034 Size: 5617 Bme1580I G_kGCm'C Cuts at: 1790 2497 2875 3678 4834 1790 Size: 707 378 803 1156 2573 Fragments arranged by size: 2573 1156 803 707 378 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5617 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3348 4306 442 Size: 416 53 83 399 24 1931 958 1753 Fragments arranged by size: 1931 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5617 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2174 2405 2716 2891 2174 Size: 231 311 175 4900 Fragments arranged by size: 4900 311 231 175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4277 1239 Size: 3038 2579 Bpu10I CC'TnA_GC Cuts at: 2774 2881 2774 Size: 107 5510 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3742 4607 4905 3742 Size: 865 298 4454 Fragments arranged by size: 4454 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2058 4280 882 Size: 617 559 2222 2219 Fragments arranged by size: 2222 2219 617 559 BsaAI yAC'GTr Cuts at: 493 1204 3408 493 Size: 711 2204 2702 Fragments arranged by size: 2702 2204 711 BsaBI GATnn'nnATC Cuts at: 5611 5611 Size: 5617 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3424 3806 4441 275 Size: 53 83 186 936 1891 382 635 1451 Fragments arranged by size: 1891 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2553 2659 4050 4448 4722 4869 5280 2553 Size: 106 1391 398 274 147 411 2890 Fragments arranged by size: 2890 1391 411 398 274 147 106 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 1178 Size: 30 165 30 825 30 4537 Fragments arranged by size: 4537 825 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2270 2921 1186 Size: 946 138 651 3882 Fragments arranged by size: 3882 946 651 138 BseYI C'CCAG_C Cuts at: 2075 2228 4820 5316 2075 Size: 153 2592 496 2376 Fragments arranged by size: 2592 2376 496 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2490 1163 Size: 573 388 366 4290 Fragments arranged by size: 4290 573 388 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3067 3828 4856 1540 Size: 275 186 1066 761 1028 2301 Fragments arranged by size: 2301 1066 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2497 2875 3678 3763 4834 2497 Size: 378 803 85 1071 3280 Fragments arranged by size: 3280 1071 803 378 85 BsmI GAATG_Cn' Cuts at: 3128 3221 3128 Size: 93 5524 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2497 2875 2936 3678 3763 4834 Size: 399 286 421 378 61 742 85 1071 Cuts at: 4834 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 421 399 378 286 85 61 BspEI T'CCGG_A Cuts at: 5280 5280 Size: 5617 BspHI T'CATG_A Cuts at: 3506 5236 3506 Size: 1730 3887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2113 2972 844 Size: 1269 859 3489 Fragments arranged by size: 3489 1269 859 BsrBI CCG'CTC Cuts at: 1394 1823 2308 3504 1394 Size: 429 485 1196 3507 Fragments arranged by size: 3507 1196 485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2356 2619 4112 4286 65 Size: 1183 1108 263 1493 174 1396 Fragments arranged by size: 1493 1396 1183 1108 263 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2553 4261 4448 1700 Size: 110 743 1708 187 2869 Fragments arranged by size: 2869 1708 743 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5617 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5617 BssSI C'ACGA_G Cuts at: 3677 4689 3677 Size: 1012 4605 BstAPI GCAn_nnn'nTGC Cuts at: 794 2468 794 Size: 1674 3943 BstBI TT'CG_AA Cuts at: 4421 4421 Size: 5617 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5617 BstYI r'GATC_y Cuts at: 1972 2545 2947 3314 3699 3716 5168 5254 5266 Size: 573 402 367 385 17 1452 86 12 Cuts at: 5266 5612 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 573 402 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2889 2889 Size: 5617 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4126 Fragments arranged by size: 4126 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4878 Fragments arranged by size: 4878 683 56 BtsI GCAGTG_nn' Cuts at: 2600 3133 3927 3947 2600 Size: 533 794 20 4270 Fragments arranged by size: 4270 794 533 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2128 2163 529 Size: 35 1564 35 3983 Fragments arranged by size: 3983 1564 35 35 DraI TTT'AAA Cuts at: 2921 2921 Size: 5617 DraIII CAC_nnn'GTG Cuts at: 2025 2135 2025 Size: 110 5507 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4624 809 Size: 507 3308 1802 Fragments arranged by size: 3308 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2801 3064 3953 Size: 54 1425 50 275 18 971 263 889 Cuts at: 3953 5582 8 Size: 1629 43 Fragments arranged by size: 1629 1425 971 889 275 263 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3064 1537 Size: 275 1252 4090 Fragments arranged by size: 4090 1252 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3547 1353 Size: 2194 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4204 4578 4724 387 Size: 1064 138 2615 374 146 1280 Fragments arranged by size: 2615 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2604 3680 4277 5064 5332 1239 Size: 393 972 1076 597 787 268 1524 Fragments arranged by size: 1524 1076 972 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2885 2885 Size: 5617 EcoRI G'AATT_C Cuts at: 2926 3304 2926 Size: 378 5239 EcoRV GAT'ATC Cuts at: 2155 2155 Size: 5617 FspI TGC'GCA Cuts at: 4123 4123 Size: 5617 HaeII r_GCGC'y Cuts at: 1678 2296 2344 4764 1678 Size: 618 48 2420 2531 Fragments arranged by size: 2531 2420 618 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 4258 4290 4332 Size: 32 135 32 656 32 1998 32 42 Cuts at: 4332 4364 1373 Size: 32 2626 Fragments arranged by size: 2626 1998 656 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 2365 2961 3207 669 Size: 988 281 226 201 596 246 3079 Fragments arranged by size: 3079 988 596 281 246 226 201 HindIII A'AGCT_T Cuts at: 748 748 Size: 5617 HpaI GTT'AAC Cuts at: 3207 3207 Size: 5617 KpnI G_GTAC'C Cuts at: 2129 2942 2129 Size: 813 4804 MfeI C'AATT_G Cuts at: 3216 3216 Size: 5617 MluI A'CGCG_T Cuts at: 5493 5493 Size: 5617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4731 4915 1305 Size: 3426 184 2007 Fragments arranged by size: 3426 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2803 10 Size: 54 1425 343 971 2824 Fragments arranged by size: 2824 1425 971 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 2822 3575 3934 4093 518 Size: 648 1590 66 753 359 159 2042 Fragments arranged by size: 2042 1590 753 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2206 2596 3033 3710 4858 5103 1606 Size: 600 390 437 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 600 437 390 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4126 Fragments arranged by size: 4126 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5617 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5617 NotI GC'GGCC_GC Cuts at: 3064 3064 Size: 5617 NspI r_CATG'y Cuts at: 1132 1229 2740 2975 4520 1132 Size: 97 1511 235 1545 2229 Fragments arranged by size: 2229 1545 1511 235 97 PasI CC'CwG_GG Cuts at: 1348 2047 1348 Size: 699 4918 PciI A'CATG_T Cuts at: 2736 4516 2736 Size: 1780 3837 PflMI CCAn_nnn'nTGG Cuts at: 1108 2135 1108 Size: 1027 4590 PmeI GTTT'AAAC Cuts at: 2921 2921 Size: 5617 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3686 3718 5199 5231 3686 Size: 32 1481 32 4072 Fragments arranged by size: 4072 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5617 PsiI TTA'TAA Cuts at: 3187 3187 Size: 5617 PstI C_TGCA'G Cuts at: 830 2969 830 Size: 2139 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5617 PvuII CAG'CTG Cuts at: 2206 2206 Size: 5617 SalI G'TCGA_C Cuts at: 1936 2959 1936 Size: 1023 4594 SbfI CC_TGCA'GG Cuts at: 2969 2969 Size: 5617 ScaI AGT'ACT Cuts at: 3865 5577 3865 Size: 1712 3905 SfcI C'TryA_G Cuts at: 826 1045 2782 2965 4100 4781 4972 826 Size: 219 1737 183 1135 681 191 1471 Fragments arranged by size: 1737 1471 1135 681 219 191 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5617 SmaI CCC'GGG Cuts at: 1872 2723 2944 1872 Size: 851 221 4545 Fragments arranged by size: 4545 851 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 3721 4622 4884 820 Size: 197 926 1778 901 262 1553 Fragments arranged by size: 1778 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3408 493 Size: 2915 2702 SpeI A'CTAG_T Cuts at: 152 2635 152 Size: 2483 3134 SphI G_CATG'C Cuts at: 1132 2975 1132 Size: 1843 3774 SspI AAT'ATT Cuts at: 5 52 3541 5 Size: 47 3489 2081 Fragments arranged by size: 3489 2081 47 StuI AGG'CCT Cuts at: 1517 2198 2798 1517 Size: 681 600 4336 Fragments arranged by size: 4336 681 600 StyI C'CwwG_G Cuts at: 513 1065 2004 3056 513 Size: 552 939 1052 3074 Fragments arranged by size: 3074 1052 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3644 3661 3814 3999 4444 325 Size: 1255 2064 17 153 185 445 1498 Fragments arranged by size: 2064 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3863 5575 96 Size: 274 80 33 51 1443 1886 1712 138 Fragments arranged by size: 1886 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5577 5609 5577 Size: 32 5585 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5617 XbaI T'CTAG_A Cuts at: 2953 2953 Size: 5617 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5617 XmaI C'CCGG_G Cuts at: 1870 2721 2942 1870 Size: 851 221 4545 Fragments arranged by size: 4545 851 221 XmnI GAAnn'nnTTC Cuts at: 2738 3746 2738 Size: 1008 4609 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3425 276 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI