(Circular) (Six-base) MAPSORT of: pFN21AE3379.seq Check: 495 from: 1 to: 8095 pFN21AE3379 8095 bp With 182 enzymes: SgfI * February 28, 2012 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5370 5370 Size: 8095 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2954 5241 5905 278 Size: 53 83 186 936 1418 2287 664 2468 Fragments arranged by size: 2468 2287 1418 936 664 186 83 53 AccI GT'mk_AC Cuts at: 1937 5438 1937 Size: 3501 4594 Acc65I G'GTAC_C Cuts at: 4377 5416 4377 Size: 1039 7056 AclI AA'CG_TT Cuts at: 1563 6222 6595 1563 Size: 4659 373 3063 Fragments arranged by size: 4659 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 4403 4712 4799 5066 6158 7542 7810 Size: 857 1914 309 87 267 1092 1384 268 Cuts at: 7810 1632 Size: 1917 Fragments arranged by size: 1917 1914 1384 1092 857 309 268 267 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7898 AflIII A'CryG_T Cuts at: 1203 3045 4540 6994 7971 1203 Size: 1842 1495 2454 977 1327 Fragments arranged by size: 2454 1842 1495 1327 977 AgeI A'CCGG_T Cuts at: 6926 6926 Size: 8095 AhdI GACnn_n'nnGTC Cuts at: 2511 3926 5218 6824 2511 Size: 1415 1292 1606 3782 Fragments arranged by size: 3782 1606 1415 1292 AleI CACnn'nnGTG Cuts at: 1166 2501 1166 Size: 1335 6760 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5298 1184 Size: 4114 3981 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4025 4057 4813 4845 4025 Size: 32 756 32 7275 Fragments arranged by size: 7275 756 32 32 AlwNI CAG_nnn'CTG Cuts at: 2888 3406 3673 4423 4969 5838 7410 2888 Size: 518 267 750 546 869 1572 3573 Fragments arranged by size: 3573 1572 869 750 546 518 267 ApaI G_GGCC'C Cuts at: 2091 3425 3973 4162 4901 5272 2091 Size: 1334 548 189 739 371 4914 Fragments arranged by size: 4914 1334 739 548 371 189 ApaLI G'TGCA_C Cuts at: 6152 7308 6152 Size: 1156 6939 ApoI r'AATT_y Cuts at: 1493 4464 5404 5630 5782 7891 1493 Size: 2971 940 226 152 2109 1697 Fragments arranged by size: 2971 2109 1697 940 226 152 AseI AT'TA_AT Cuts at: 160 1032 6649 160 Size: 872 5617 1606 Fragments arranged by size: 5617 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8095 AvaI C'yCGr_G Cuts at: 1870 1943 2925 3651 3965 4325 5420 1870 Size: 73 982 726 314 360 1095 4545 Fragments arranged by size: 4545 1095 982 726 360 314 73 BamHI G'GATC_C Cuts at: 4938 5425 5792 4938 Size: 487 367 7241 Fragments arranged by size: 7241 487 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 3053 3556 4377 4514 Size: 325 217 627 995 271 503 821 137 Cuts at: 4514 5096 5416 6871 618 Size: 582 320 1455 1842 Fragments arranged by size: 1842 1455 995 821 627 582 503 325 320 271 217 137 BanII G_rGCy'C Cuts at: 1391 2052 2091 2196 2493 2724 3063 3425 3570 Size: 661 39 105 297 231 339 362 145 Cuts at: 3570 3758 3787 3973 4162 4203 4321 4491 4857 Size: 188 29 186 189 41 118 170 366 Cuts at: 4857 4901 5272 5414 1391 Size: 44 371 142 4072 Fragments arranged by size: 4072 661 371 366 362 339 297 231 189 188 186 170 145 142 118 105 44 41 39 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2311 3340 4797 4845 928 Size: 971 412 1029 1457 48 4178 Fragments arranged by size: 4178 1457 1029 971 412 48 BbvCI CC'TCA_GC Cuts at: 3198 3198 Size: 8095 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6286 6320 1903 Size: 34 50 34 4265 34 3678 Fragments arranged by size: 4265 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5987 7197 5987 Size: 1210 6885 BclI T'GATC_A Cuts at: 1548 1548 Size: 8095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2719 3075 3939 Size: 107 122 71 631 605 1047 356 864 Cuts at: 3939 3992 4448 4457 4562 6706 136 Size: 53 456 9 105 2144 1525 Fragments arranged by size: 2144 1525 1047 864 631 605 456 356 122 107 105 71 53 9 BglII A'GATC_T Cuts at: 8090 8090 Size: 8095 BlpI GC'TnA_GC Cuts at: 2487 5150 5512 2487 Size: 2663 362 5070 Fragments arranged by size: 5070 2663 362 Bme1580I G_kGCm'C Cuts at: 1790 2091 2787 3425 3973 4162 4517 4901 5272 Size: 301 696 638 548 189 355 384 371 Cuts at: 5272 6156 7312 1790 Size: 884 1156 2573 Fragments arranged by size: 2573 1156 884 696 638 548 384 371 355 301 189 BmgBI CAC'GTC Cuts at: 1318 4057 5179 1318 Size: 2739 1122 4234 Fragments arranged by size: 4234 2739 1122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 3172 3328 Size: 416 53 83 399 24 1388 367 156 Cuts at: 3328 4556 5826 6784 442 Size: 1228 1270 958 1753 Fragments arranged by size: 1753 1388 1270 1228 958 416 399 367 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8095 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2608 3589 4340 5032 5214 5319 2215 Size: 393 981 751 692 182 105 4991 Fragments arranged by size: 4991 981 751 692 393 182 105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2196 2357 2626 2630 2740 2829 2997 Size: 890 67 161 269 4 110 89 168 Cuts at: 2997 3134 3158 3899 4277 4445 4904 6755 1239 Size: 137 24 741 378 168 459 1851 2579 Fragments arranged by size: 2579 1851 890 741 459 378 269 168 168 161 137 110 89 67 24 4 Bpu10I CC'TnA_GC Cuts at: 2345 3009 3198 3674 3757 5144 2345 Size: 664 189 476 83 1387 5296 Fragments arranged by size: 5296 1387 664 476 189 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4690 6220 7085 7383 4690 Size: 1530 865 298 5402 Fragments arranged by size: 5402 1530 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2267 2758 2989 3466 4709 6758 882 Size: 617 768 491 231 477 1243 2049 2219 Fragments arranged by size: 2219 2049 1243 768 617 491 477 231 BsaAI yAC'GTr Cuts at: 493 1204 3048 3546 3736 4477 5886 493 Size: 711 1844 498 190 741 1409 2702 Fragments arranged by size: 2702 1844 1409 741 711 498 190 BsaBI GATnn'nnATC Cuts at: 8089 8089 Size: 8095 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2951 3687 3894 5238 Size: 53 83 186 936 1418 736 207 1344 Cuts at: 5238 5330 5902 6284 6919 275 Size: 92 572 382 635 1451 Fragments arranged by size: 1451 1418 1344 936 736 635 572 382 207 186 92 83 53 BsaWI w'CCGG_w Cuts at: 5209 6528 6926 7200 7347 7758 5209 Size: 1319 398 274 147 411 5546 Fragments arranged by size: 5546 1319 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4025 4055 4473 4503 1178 Size: 30 165 30 2622 30 418 30 4770 Fragments arranged by size: 4770 2622 418 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 3133 3524 3623 3626 3785 4469 4754 Size: 1279 668 391 99 3 159 684 285 Cuts at: 4754 1186 Size: 4527 Fragments arranged by size: 4527 1279 684 668 391 285 159 99 3 BseYI C'CCAG_C Cuts at: 2030 2331 2897 3259 3293 3937 4003 4130 4280 Size: 301 566 362 34 644 66 127 150 Cuts at: 4280 4847 4894 5040 5271 7298 7794 2030 Size: 567 47 146 231 2027 496 2331 Fragments arranged by size: 2331 2027 644 567 566 496 362 301 231 150 146 127 66 47 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3122 3521 3966 4418 4829 1163 Size: 573 1386 399 445 452 411 4429 Fragments arranged by size: 4429 1386 573 452 445 411 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3233 3444 4498 5545 6306 7334 Size: 275 186 1232 211 1054 1047 761 1028 Cuts at: 7334 1540 Size: 2301 Fragments arranged by size: 2301 1232 1054 1047 1028 761 275 211 186 BsiHKAI G_wGCw'C Cuts at: 2196 2946 3225 3758 3774 4203 4491 4857 6156 Size: 750 279 533 16 429 288 366 1299 Cuts at: 6156 6241 7312 2196 Size: 85 1071 2979 Fragments arranged by size: 2979 1299 1071 750 533 429 366 288 279 85 16 BsmI GAATG_Cn' Cuts at: 5606 5699 5606 Size: 93 8002 BsmBI CGTCTCn'nnnn_ Cuts at: 5306 5306 Size: 8095 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2091 2196 2493 2724 2787 2946 Size: 399 262 39 105 297 231 63 159 Cuts at: 2946 3063 3225 3425 3570 3758 3774 3787 3973 Size: 117 162 200 145 188 16 13 186 Cuts at: 3973 4162 4203 4321 4491 4517 4857 4901 5272 Size: 189 41 118 170 26 340 44 371 Cuts at: 5272 5414 6156 6241 7312 1391 Size: 142 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 340 297 262 231 200 189 188 186 170 162 159 145 142 118 117 105 85 63 44 41 39 26 16 13 BspEI T'CCGG_A Cuts at: 5209 7758 5209 Size: 2549 5546 BspHI T'CATG_A Cuts at: 5984 7714 5984 Size: 1730 6365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2901 2920 5370 5450 844 Size: 2057 19 2450 80 3489 Fragments arranged by size: 3489 2450 2057 80 19 BsrBI CCG'CTC Cuts at: 1394 1823 3033 3142 5982 1394 Size: 429 1210 109 2840 3507 Fragments arranged by size: 3507 2840 1210 429 109 BsrDI GCAATG_nn' Cuts at: 65 1248 3364 4151 6590 6764 65 Size: 1183 2116 787 2439 174 1396 Fragments arranged by size: 2439 2116 1396 1183 787 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2850 3013 3225 3324 3553 3559 3593 Size: 110 1040 163 212 99 229 6 34 Cuts at: 3593 3804 6739 6926 1700 Size: 211 2935 187 2869 Fragments arranged by size: 2935 2869 1040 229 212 211 187 163 110 99 34 6 BsrGI T'GTAC_A Cuts at: 96 5157 96 Size: 5061 3034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8095 BssSI C'ACGA_G Cuts at: 3243 3434 4445 4631 5199 6155 7167 3243 Size: 191 1011 186 568 956 1012 4171 Fragments arranged by size: 4171 1012 1011 956 568 191 186 BstAPI GCAn_nnn'nTGC Cuts at: 794 3406 4366 794 Size: 2612 960 4523 Fragments arranged by size: 4523 2612 960 BstBI TT'CG_AA Cuts at: 6899 6899 Size: 8095 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8095 BstXI CCAn_nnnn'nTGG Cuts at: 2083 2083 Size: 8095 BstYI r'GATC_y Cuts at: 1972 3094 3403 4938 5425 5792 6177 6194 7646 Size: 1122 309 1535 487 367 385 17 1452 Cuts at: 7646 7732 7744 8090 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1535 1452 1122 487 385 367 346 309 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2673 2673 Size: 8095 BtgI C'CryG_G Cuts at: 513 1065 2076 2716 2773 2827 3714 4100 513 Size: 552 1011 640 57 54 887 386 4508 Fragments arranged by size: 4508 1011 887 640 552 386 57 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2139 4085 5336 487 Size: 683 56 913 1946 1251 3246 Fragments arranged by size: 3246 1946 1251 913 683 56 BtsI GCAGTG_nn' Cuts at: 2561 3035 3306 3858 4403 4435 5611 6405 6425 Size: 474 271 552 545 32 1176 794 20 Cuts at: 6425 2561 Size: 4231 Fragments arranged by size: 4231 1176 794 552 545 474 271 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8095 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8060 DraI TTT'AAA Cuts at: 5399 5399 Size: 8095 DraIII CAC_nnn'GTG Cuts at: 2069 2069 Size: 8095 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 5012 7102 809 Size: 507 999 2697 2090 1802 Fragments arranged by size: 2697 2090 1802 999 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2596 2852 3150 Size: 54 1425 50 275 18 766 256 298 Cuts at: 3150 3230 3322 3441 3591 3806 3984 4074 4495 Size: 80 92 119 150 215 178 90 421 Cuts at: 4495 5542 6431 8060 8 Size: 1047 889 1629 43 Fragments arranged by size: 1629 1425 1047 889 766 421 298 275 256 215 178 150 119 92 90 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3230 3441 4495 5542 1537 Size: 275 1418 211 1054 1047 4090 Fragments arranged by size: 4090 1418 1054 1047 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2265 3416 3464 4229 6025 1353 Size: 825 87 1151 48 765 1796 3423 Fragments arranged by size: 3423 1796 1151 825 765 87 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3578 6682 7056 7202 387 Size: 1064 138 1989 3104 374 146 1280 Fragments arranged by size: 3104 1989 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 3756 4201 4489 4855 2194 Size: 1562 445 288 366 5434 Fragments arranged by size: 5434 1562 445 366 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2196 2357 2489 2626 2630 2740 Size: 393 497 67 161 132 137 4 110 Cuts at: 2740 2829 2997 3134 3158 3899 4277 4403 4445 Size: 89 168 137 24 741 378 126 42 Cuts at: 4445 4712 4799 4904 5066 6158 6755 7542 7810 Size: 267 87 105 162 1092 597 787 268 Cuts at: 7810 1239 Size: 1524 Fragments arranged by size: 1524 1092 787 741 597 497 393 378 268 267 168 162 161 137 137 132 126 110 105 89 87 67 42 24 4 EcoNI CCTnn'n_nnAGG Cuts at: 2348 3086 3197 3677 4031 4905 2348 Size: 738 111 480 354 874 5538 Fragments arranged by size: 5538 874 738 480 354 111 EcoO109I rG'GnC_Cy Cuts at: 2034 2087 2088 2341 2678 3871 3969 3970 4062 Size: 53 1 253 337 1193 98 1 92 Cuts at: 4062 4158 4159 4239 4870 4898 4910 5116 5268 Size: 96 1 80 631 28 12 206 152 Cuts at: 5268 2034 Size: 4861 Fragments arranged by size: 4861 1193 631 337 253 206 152 98 96 92 80 53 28 12 1 1 1 EcoRI G'AATT_C Cuts at: 5404 5782 5404 Size: 378 7717 FseI GG_CCGG'CC Cuts at: 3597 3597 Size: 8095 FspI TGC'GCA Cuts at: 6601 6601 Size: 8095 HaeII r_GCGC'y Cuts at: 1678 4688 7242 1678 Size: 3010 2554 2531 Fragments arranged by size: 3010 2554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2497 2529 2808 2840 3912 Size: 32 135 32 925 32 279 32 1072 Cuts at: 3912 3944 5204 5236 6736 6768 6810 6842 1373 Size: 32 1260 32 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 1260 1072 925 279 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5439 5685 669 Size: 988 281 3501 246 3079 Fragments arranged by size: 3501 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2993 4802 5152 748 Size: 2245 1809 350 3691 Fragments arranged by size: 3691 2245 1809 350 HpaI GTT'AAC Cuts at: 5685 5685 Size: 8095 KpnI G_GTAC'C Cuts at: 4381 5420 4381 Size: 1039 7056 MfeI C'AATT_G Cuts at: 5694 5694 Size: 8095 MluI A'CGCG_T Cuts at: 7971 7971 Size: 8095 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3012 4114 7209 7393 1305 Size: 1707 1102 3095 184 2007 Fragments arranged by size: 3095 2007 1707 1102 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3152 3986 4076 10 Size: 54 1425 343 1320 834 90 4029 Fragments arranged by size: 4029 1425 1320 834 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2154 2501 3083 6053 6412 6571 518 Size: 648 988 347 582 2970 359 159 2042 Fragments arranged by size: 2970 2042 988 648 582 359 347 159 MspA1I CmG'CkG Cuts at: 1606 2335 2486 2975 3178 4102 4308 4420 4644 Size: 729 151 489 203 924 206 112 224 Cuts at: 4644 4847 5044 5511 6188 7336 7581 1606 Size: 203 197 467 677 1148 245 2120 Fragments arranged by size: 2120 1148 924 729 677 489 467 245 224 206 203 203 197 151 112 NaeI GCC'GGC Cuts at: 3326 3595 3326 Size: 269 7826 NcoI C'CATG_G Cuts at: 513 1065 2076 2716 2773 3714 513 Size: 552 1011 640 57 941 4894 Fragments arranged by size: 4894 1011 941 640 552 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 8095 NgoMIV G'CCGG_C Cuts at: 3324 3593 3324 Size: 269 7826 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8095 NotI GC'GGCC_GC Cuts at: 3230 5542 3230 Size: 2312 5783 NspI r_CATG'y Cuts at: 1132 1229 2905 4544 5453 6998 1132 Size: 97 1676 1639 909 1545 2229 Fragments arranged by size: 2229 1676 1639 1545 909 97 PasI CC'CwG_GG Cuts at: 1348 3418 1348 Size: 2070 6025 PciI A'CATG_T Cuts at: 4540 6994 4540 Size: 2454 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8095 PfoI T'CCnGG_A Cuts at: 2322 3798 5352 2322 Size: 1476 1554 5065 Fragments arranged by size: 5065 1554 1476 PmeI GTTT'AAAC Cuts at: 5399 5399 Size: 8095 PmlI CAC'GTG Cuts at: 3546 4477 3546 Size: 931 7164 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 4730 4762 6164 6196 7677 7709 4025 Size: 32 673 32 1402 32 1481 32 4411 Fragments arranged by size: 4411 1481 1402 673 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2034 2678 4062 4870 2034 Size: 644 1384 808 5259 Fragments arranged by size: 5259 1384 808 644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8095 PsiI TTA'TAA Cuts at: 5665 5665 Size: 8095 PspOMI G'GGCC_C Cuts at: 2087 3421 3969 4158 4897 5268 2087 Size: 1334 548 189 739 371 4914 Fragments arranged by size: 4914 1334 739 548 371 189 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5237 5269 5237 Size: 32 8063 PstI C_TGCA'G Cuts at: 830 3042 3402 4395 4965 5447 830 Size: 2212 360 993 570 482 3478 Fragments arranged by size: 3478 2212 993 570 482 360 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8095 PvuII CAG'CTG Cuts at: 2335 2486 2975 3178 4420 4644 4847 5044 2335 Size: 151 489 203 1242 224 203 197 5386 Fragments arranged by size: 5386 1242 489 224 203 203 197 151 RsrII CG'GwC_CG Cuts at: 3551 3551 Size: 8095 SacI G_AGCT'C Cuts at: 2196 3758 4203 4491 4857 2196 Size: 1562 445 288 366 5434 Fragments arranged by size: 5434 1562 445 366 288 SacII CC_GC'GG Cuts at: 4103 4103 Size: 8095 SalI G'TCGA_C Cuts at: 1936 5437 1936 Size: 3501 4594 SanDI GG'GwC_CC Cuts at: 2678 2678 Size: 8095 SapI GCTCTTCn'nnn_ Cuts at: 3416 3416 Size: 8095 SbfI CC_TGCA'GG Cuts at: 3402 5447 3402 Size: 2045 6050 ScaI AGT'ACT Cuts at: 6343 8055 6343 Size: 1712 6383 SfcI C'TryA_G Cuts at: 826 1045 3038 3398 4028 4391 4961 5443 6578 Size: 219 1993 360 630 363 570 482 1135 Cuts at: 6578 7259 7450 826 Size: 681 191 1471 Fragments arranged by size: 1993 1471 1135 681 630 570 482 363 360 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3939 3992 3939 Size: 53 8042 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8095 SgrAI Cr'CCGG_yG Cuts at: 1700 3225 3559 1700 Size: 1525 334 6236 Fragments arranged by size: 6236 1525 334 SmaI CCC'GGG Cuts at: 1872 3653 3967 5422 1872 Size: 1781 314 1455 4545 Fragments arranged by size: 4545 1781 1455 314 SmlI C'TyrA_G Cuts at: 820 1017 1943 4669 6199 7100 7362 820 Size: 197 926 2726 1530 901 262 1553 Fragments arranged by size: 2726 1553 1530 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5886 493 Size: 5393 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8095 SphI G_CATG'C Cuts at: 1132 2905 5453 1132 Size: 1773 2548 3774 Fragments arranged by size: 3774 2548 1773 SspI AAT'ATT Cuts at: 5 52 6019 5 Size: 47 5967 2081 Fragments arranged by size: 5967 2081 47 StuI AGG'CCT Cuts at: 1517 3192 4556 1517 Size: 1675 1364 5056 Fragments arranged by size: 5056 1675 1364 StyI C'CwwG_G Cuts at: 513 1065 2053 2076 2716 2773 3184 3216 3714 Size: 552 988 23 640 57 411 32 498 Cuts at: 3714 4226 4557 5052 5534 513 Size: 512 331 495 482 3074 Fragments arranged by size: 3074 988 640 552 512 498 495 482 411 331 57 32 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2975 4110 5084 6122 6139 6292 6477 Size: 1255 1395 1135 974 1038 17 153 185 Cuts at: 6477 6922 325 Size: 445 1498 Fragments arranged by size: 1498 1395 1255 1135 1038 974 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4543 5157 5244 Size: 274 80 33 51 1443 2566 614 87 Cuts at: 5244 6341 8053 96 Size: 1097 1712 138 Fragments arranged by size: 2566 1712 1443 1097 614 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2936 2968 4791 4823 8055 8087 2936 Size: 32 1823 32 3232 32 2944 Fragments arranged by size: 3232 2944 1823 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3344 1316 Size: 2028 6067 XbaI T'CTAG_A Cuts at: 5431 5431 Size: 8095 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2080 2011 Size: 69 8026 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8095 XmaI C'CCGG_G Cuts at: 1870 3651 3965 5420 1870 Size: 1781 314 1455 4545 Fragments arranged by size: 4545 1781 1455 314 XmnI GAAnn'nnTTC Cuts at: 6224 6224 Size: 8095 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2952 5239 5903 276 Size: 53 83 186 936 1418 2287 664 2468 Fragments arranged by size: 2468 2287 1418 936 664 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PspXI SexAI SfoI SrfI SwaI