################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 184 GTCTAC GT^[AC][GT]AC AflIII 426 ACATGT A^C[GA][CT]GT Aor51HI 83 AGCGCT AGC^GCT ApaI 177 GGGCCC GGGCC^C AspHI 333 GTGCTC G[AT]GC[AT]^C BanII 177 GGGCCC G[GA]GC[CT]^C BsaI 412 GAGACC GGTCTC(1/5) BsgI 268 CTGCAC GTGCAG(16/14) BsiHKAI 333 GTGCTC G[AT]GC[AT]^C BsmAI 412 GAGAC GTCTC(1/5) BspLU11I 426 ACATGT A^CATGT BsrFI 212 GCCGGT [GA]^CCGG[CT] BsrI 301 CCAGT ACTGG(1/-1) BstEII 318 GGTCACC G^GT[ACGT]ACC BstPI 318 GGTCACC G^GT[ACGT]ACC CfoI 84 GCGC GCG^C Cfr10I 212 GCCGGT [GA]^CCGG[CT] DdeI 8 CTGAG C^T[ACGT]AG Eco47III 83 AGCGCT AGC^GCT EcoO65I 318 GGTCACC G^GT[ACGT]ACC HaeII 83 AGCGCT [GA]GCGC^[CT] HhaI 84 GCGC GCG^C HinP1I 84 GCGC G^CGC HindIII 44 AAGCTT A^AGCTT HinfI 194 GAGTC G^A[ACGT]TC MaeII 110 ACGT A^CGT MaeIII 319 GTCAC ^GT[ACGT]AC MboII 263 TCTTC GAAGA(8/7) MspA1I 242 CAGCTG C[AC]G^C[GT]G MwoI 77 GCCGTGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 225 CCATGG C^CATGG NspI 426 ACATGT [GA]CATG^[CT] PflMI 241 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 194 GAGTC GAGTC(4/5) PpuMI 145 AGGACCT [GA]G^G[AT]CC[CT] PstI 361 CTGCAG CTGCA^G PvuII 242 CAGCTG CAG^CTG SexAI 415 ACCTGGT A^CC[AT]GGT SfcI 361 CTGCAG C^T[GA][CT]AG Tsp45I 319 GTCAC ^GT[GC]AC Van91I 241 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspI 313 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 414 GACCTGGTC BanI 393 GGCACC G^G[CT][GA]CC 400 GGCACC BbvI 57 GCTGC GCAGC(8/12) 360 GCTGC BmyI 177 GGGCCC G[AGT]GC[ACT]^C 333 GTGCTC BsmFI 115 GTCCC GGGAC(10/14) 298 GTCCC Bsp1286I 177 GGGCCC G[AGT]GC[ACT]^C 333 GTGCTC BstF5I 92 CATCC GGATG(2/0) 339 CATCC Cac8I 165 GCGGGC GC[ACGT]^[ACGT]GC 363 GCAGGC DsaI 225 CCATGG C^C[GA][CT]GG 368 CCGTGG EcoT14I 218 CCAAGG C^C[AT][AT]GG 225 CCATGG FauI 102 GCGGG CCCGC(4/6) 165 GCGGG FokI 92 CATCC GGATG(9/13) 339 CATCC HapII 138 CCGG C^CGG 213 CCGG HpaII 138 CCGG C^CGG 213 CCGG MspI 138 CCGG C^CGG 213 CCGG PflFI 313 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 414 GACCTGGTC StyI 218 CCAAGG C^C[AT][AT]GG 225 CCATGG TseI 57 GCTGC G^C[AT]GC 360 GCTGC TspRI 125 CACTG CA[GC]TG[ACGT][ACGT]^ 302 CAGTG Tth111I 313 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 414 GACCTGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI AluI AvaII BsaJI BsiYI BslI BssKI BstNI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI MnlI MvaI NlaIII NlaIV Sau96I ScrFI