(Linear) (Six-base) MAPSORT of: KIEE1632.seq  Check: 5241  from: 1  to: 441



KIEE1632.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AccI GT'mk_AC

Cuts at:      0     185     441 
   Size:        185     256
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     441 
   Size:         32     409
 
AfeI AGC'GCT

Cuts at:      0      85     441 
   Size:         85     356
 
AflIII A'CryG_T

Cuts at:      0     426     441 
   Size:        426      15
 
ApaI G_GGCC'C

Cuts at:      0     181     441 
   Size:        181     260
 
BanI G'GyrC_C

Cuts at:      0     393     400     441 
   Size:        393       7      41

  Fragments arranged by size: 

                 393      41       7
 
BanII G_rGCy'C

Cuts at:      0     181     441 
   Size:        181     260
 
Bme1580I G_kGCm'C

Cuts at:      0     181     441 
   Size:        181     260
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     295     441 
   Size:        295     146
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     406     441 
   Size:        406      35
 
BseYI C'CCAG_C

Cuts at:      0     240     441 
   Size:        240     201
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     441 
   Size:        253     188
 
BsiHKAI G_wGCw'C

Cuts at:      0     337     441 
   Size:        337     104
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     337     441 
   Size:        181     156     104

  Fragments arranged by size: 

                 181     156     104
 
BsrFI r'CCGG_y

Cuts at:      0     212     441 
   Size:        212     229
 
BstEII G'GTnAC_C

Cuts at:      0     318     441 
   Size:        318     123
 
BtgI C'CryG_G

Cuts at:      0     225     368     441 
   Size:        225     143      73

  Fragments arranged by size: 

                 225     143      73
 
BtsI GCAGTG_nn'

Cuts at:      0     124     441 
   Size:        124     317
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     441 
   Size:         32     409
 
EcoO109I rG'GnC_Cy

Cuts at:      0     146     177     178     441 
   Size:        146      31       1     263

  Fragments arranged by size: 

                 263     146      31       1
 
HaeII r_GCGC'y

Cuts at:      0      87     441 
   Size:         87     354
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     403     416     435     441 
   Size:        403      13      19       6

  Fragments arranged by size: 

                 403      19      13       6
 
HindIII A'AGCT_T

Cuts at:      0      44     441 
   Size:         44     397
 
MspA1I CmG'CkG

Cuts at:      0     244     441 
   Size:        244     197
 
NcoI C'CATG_G

Cuts at:      0     225     441 
   Size:        225     216
 
NspI r_CATG'y

Cuts at:      0     430     441 
   Size:        430      11
 
PciI A'CATG_T

Cuts at:      0     426     441 
   Size:        426      15
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     247     441 
   Size:        247     194
 
PpuMI rG'GwC_Cy

Cuts at:      0     146     441 
   Size:        146     295
 
PspOMI G'GGCC_C

Cuts at:      0     177     441 
   Size:        177     264
 
PstI C_TGCA'G

Cuts at:      0     365     441 
   Size:        365      76
 
PvuII CAG'CTG

Cuts at:      0     244     441 
   Size:        244     197
 
SexAI A'CCwGG_T

Cuts at:      0     415     441 
   Size:        415      26
 
SfcI C'TryA_G

Cuts at:      0     361     441 
   Size:        361      80
 
StyI C'CwwG_G

Cuts at:      0     218     225     441 
   Size:        218       7     216

  Fragments arranged by size: 

                 218     216       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      52     360     441 
   Size:         52     308      81

  Fragments arranged by size: 

                 308      81      52
 
Tth111I GACn'n_nGTC

Cuts at:      0     316     417     441 
   Size:        316     101      24

  Fragments arranged by size: 

                 316     101      24
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     ApaI     BanI    BanII Bme1580I
     BmrI     BsaI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI   BstEII
     BtgI     BtsI  Eco57MI EcoO109I    HaeII    Hin4I  HindIII   MspA1I
     NcoI     NspI     PciI    PflMI    PpuMI   PspOMI     PstI    PvuII
    SexAI     SfcI     StyI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI