################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BciVI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AfaI 177 GTAC GT^AC AlwI 471 GATCC GGATC(4/5) AspHI 592 GTGCTC G[AT]GC[AT]^C BbsI 307 GTCTTC GAAGAC(2/6) BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BcgI 537 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 158 CCCGG CC^[GC]GG BfaI 224 CTAG C^TAG BlpI 758 GCTCAGC GC^T[ACGT]AGC Bpu1102I 758 GCTCAGC GC^T[ACGT]AGC BpuAI 307 GTCTTC GAAGAC(2/6) BsaWI 512 ACCGGA [AT]^CCGG[AT] BsgI 606 CTGCAC GTGCAG(16/14) BsiHKAI 592 GTGCTC G[AT]GC[AT]^C BsmAI 578 GTCTC GTCTC(1/5) BspHI 404 TCATGA T^CATGA BsrBI 684 CCGCTC CCGCTC(-3/-3) BsrDI 660 CATTGC GCAATG(2/0) BsrFI 380 GCCGGC [GA]^CCGG[CT] CelII 758 GCTCAGC GC^T[ACGT]AGC CfoI 896 GCGC GCG^C Cfr10I 380 GCCGGC [GA]^CCGG[CT] DraIII 327 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 848 CCATGG C^C[GA][CT]GG EaeI 785 CGGCCA [CT]^GGCC[GA] EcoNI 531 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 146 CCCGC CCCGC(4/6) HhaI 896 GCGC GCG^C HinP1I 896 GCGC G^CGC HincII 477 GTCAAC GT[CT]^[GA]AC HindII 477 GTCAAC GT[CT]^[GA]AC MaeI 224 CTAG C^TAG MaeIII 841 GTCAC ^GT[ACGT]AC MspA1I 64 CAGCTG C[AC]G^C[GT]G NaeI 380 GCCGGC GCC^GGC NciI 158 CCCGG CC^[GC]GG NcoI 848 CCATGG C^CATGG NgoMIV 380 GCCGGC G^CCGGC PshAI 571 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 64 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA RsaI 177 GTAC GT^AC SpeI 223 ACTAGT A^CTAGT Tsp45I 841 GTCAC ^GT[GC]AC Tsp509I 250 AATT ^AATT XcmI 548 CCATGATCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BanI 509 GGCACC G^G[CT][GA]CC 540 GGTGCC BclI 245 TGATCA T^GATCA 551 TGATCA BmyI 508 GGGCAC G[AGT]GC[ACT]^C 592 GTGCTC BpmI 67 CTGGAG CTGGAG(16/14) 151 CTGGAG BsaAI 367 TACGTG [CT]AC^GT[GA] 717 TACGTG BseRI 198 CTCCTC GAGGAG(10/8) 903 GAGGAG Bsp1286I 508 GGGCAC G[AGT]GC[ACT]^C 592 GTGCTC BsrI 558 ACTGG ACTGG(1/-1) 474 CCAGT EcoT14I 203 CCTTGG C^C[AT][AT]GG 848 CCATGG FbaI 245 TGATCA T^GATCA 551 TGATCA HinfI 527 GATTC G^A[ACGT]TC 855 GAATC MaeII 368 ACGT A^CGT 718 ACGT PflMI 499 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 729 CCATCTGCTGG SfaNI 335 GCATC GCATC(5/9) 635 GCATC StyI 203 CCTTGG C^C[AT][AT]GG 848 CCATGG TfiI 527 GATTC G^A[AT]TC 855 GAATC Van91I 499 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 729 CCATCTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TseI TspRI