################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BalI BamHI BbrPI BbsI BbvCI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HindIII HpaI KpnI Ksp632I MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 633 GTAC GT^AC AocI 909 CCTCAGG CC^T[ACGT]AGG Aor51HI 443 AGCGCT AGC^GCT BbeI 754 GGCGCC GGCGC^C BcgI 789 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 101 GGATAC GTATCC(6/5) BclI 254 TGATCA T^GATCA BglI 907 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 11 CTGGAG CTGGAG(16/14) BsmBI 436 CGTCTC CGTCTC(1/5) BspMI 226 GCAGGT ACCTGC(4/8) BsrDI 423 CATTGC GCAATG(2/0) BsrI 635 ACTGG ACTGG(1/-1) BstYI 527 GGATCT [GA]^GATC[CT] Bsu36I 909 CCTCAGG CC^T[ACGT]AGG DraIII 385 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 610 GACTCGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 882 CCGCGG C^C[GA][CT]GG Eco47III 443 AGCGCT AGC^GCT Eco81I 909 CCTCAGG CC^T[ACGT]AGG FbaI 254 TGATCA T^GATCA HincII 75 GTCGAC GT[CT]^[GA]AC HindII 75 GTCGAC GT[CT]^[GA]AC KasI 754 GGCGCC G^GCGCC KspI 882 CCGCGG CCGC^GG MflI 527 GGATCT [GA]^GATC[CT] NarI 754 GGCGCC GG^CGCC NspI 671 ACATGC [GA]CATG^[CT] PaeR7I 439 CTCGAG C^TCGAG RsaI 633 GTAC GT^AC SacII 882 CCGCGG CCGC^GG SalI 75 GTCGAC G^TCGAC SfoI 754 GGCGCC GGC^GCC SmaI 814 CCCGGG CCC^GGG SmlI 439 CTCGAG C^T[CT][GA]AG XhoI 439 CTCGAG C^TCGAG XhoII 527 GGATCT [GA]^GATC[CT] XmaCI 814 CCCGGG C^CCGGG XmaI 814 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 75 GTCGAC GT^[AC][GT]AC 820 GTCTAC AcyI 72 GGCGTC G[GA]^CG[CT]C 754 GGCGCC AlwI 188 GGATC GGATC(4/5) 527 GGATC ApaI 729 GGGCCC GGGCC^C 905 GGGCCC AvaI 439 CTCGAG C^[CT]CG[GA]G 814 CCCGGG AviII 223 TGCGCA TGC^GCA 837 TGCGCA BsaHI 72 GGCGTC G[GA]^CG[CT]C 754 GGCGCC BsgI 416 GTGCAG GTGCAG(16/14) 874 GTGCAG BsmAI 41 GTCTC GTCTC(1/5) 437 GTCTC BsoBI 439 CTCGAG C^[CT]CG[GA]G 814 CCCGGG BsrFI 682 GCCGGC [GA]^CCGG[CT] 769 GCCGGC BssHII 888 GCGCGC G^CGCGC 943 GCGCGC BstAPI 418 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 867 GCAAGGGGTGC BstXI 201 CCAGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 924 CCAGGGCTCTGG Cfr10I 682 GCCGGC [GA]^CCGG[CT] 769 GCCGGC EaeI 95 CGGCCA [CT]^GGCC[GA] 767 TGGCCG FspI 223 TGCGCA TGC^GCA 837 TGCGCA HgaI 73 GCGTC GACGC(5/10) 435 GCGTC Hin1I 72 GGCGTC G[GA]^CG[CT]C 754 GGCGCC HinfI 610 GACTC G^A[ACGT]TC 721 GACTC MboII 204 GAAGA GAAGA(8/7) 858 GAAGA NaeI 682 GCCGGC GCC^GGC 769 GCCGGC NgoMIV 682 GCCGGC G^CCGGC 769 GCCGGC NlaIII 672 CATG CATG^ 738 CATG PleI 610 GACTC GAGTC(4/5) 721 GACTC PstI 583 CTGCAG CTGCA^G 621 CTGCAG SfcI 583 CTGCAG C^T[GA][CT]AG 621 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BanI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PflMI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I Van91I