(Circular) (Six-base) MAPSORT of: pFN21AE5441.seq Check: 5839 from: 1 to: 5656 pFN21AE5441 5656 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2081 2826 2999 1937 Size: 144 745 173 4594 Fragments arranged by size: 4594 745 173 144 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 3719 5103 5371 1632 Size: 986 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1101 986 268 AfeI AGC'GCT Cuts at: 2450 2450 Size: 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2143 2253 2587 2596 3399 4971 2143 Size: 110 334 9 803 1572 2828 Fragments arranged by size: 2828 1572 803 334 110 9 ApaI G_GGCC'C Cuts at: 2738 2914 2738 Size: 176 5480 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2444 2819 2981 1870 Size: 73 501 375 162 4545 Fragments arranged by size: 4545 501 375 162 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2414 2759 2920 2977 Size: 325 217 627 264 363 345 161 57 Cuts at: 2977 4432 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 363 345 325 264 217 161 57 BanII G_rGCy'C Cuts at: 1391 2388 2738 2914 2936 2975 1391 Size: 997 350 176 22 39 4072 Fragments arranged by size: 4072 997 350 176 39 22 BbeI G_GCGC'C Cuts at: 2763 2763 Size: 5656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2783 2817 3847 3881 1903 Size: 34 846 34 1030 34 3678 Fragments arranged by size: 3678 1030 846 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2100 3548 4758 2100 Size: 1448 1210 2998 Fragments arranged by size: 2998 1448 1210 BclI T'GATC_A Cuts at: 1548 2259 1548 Size: 711 4945 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2918 4267 136 Size: 107 122 71 631 605 1246 1349 1525 Fragments arranged by size: 1525 1349 1246 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2738 2914 2923 3717 4873 1790 Size: 948 176 9 794 1156 2573 Fragments arranged by size: 2573 1156 948 794 176 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 4316 1239 Size: 798 2279 2579 Fragments arranged by size: 2579 2279 798 Bpu10I CC'TnA_GC Cuts at: 2898 2898 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2760 3463 3845 Size: 53 83 186 936 545 682 703 382 Cuts at: 3845 4480 275 Size: 635 1451 Fragments arranged by size: 1451 936 703 682 635 545 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5656 BseYI C'CCAG_C Cuts at: 2026 2119 2136 2253 2320 2335 2541 2787 4859 Size: 93 17 117 67 15 206 246 2072 Cuts at: 4859 5355 2026 Size: 496 2327 Fragments arranged by size: 2327 2072 496 246 206 117 93 67 17 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 2900 1163 Size: 573 706 458 3919 Fragments arranged by size: 3919 706 573 458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3717 3802 4873 3717 Size: 85 1071 4500 Fragments arranged by size: 4500 1071 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 BsmBI CGTCTCn'nnnn_ Cuts at: 2447 2447 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2388 2738 2914 2923 2936 2975 3717 Size: 399 598 350 176 9 13 39 742 Cuts at: 3717 3802 4873 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 598 399 350 176 85 39 13 9 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2222 3011 844 Size: 1378 789 3489 Fragments arranged by size: 3489 1378 789 BsrBI CCG'CTC Cuts at: 1394 1823 2470 2707 2850 3543 1394 Size: 429 647 237 143 693 3507 Fragments arranged by size: 3507 693 647 429 237 143 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 4151 4325 65 Size: 1183 1179 1724 174 1396 Fragments arranged by size: 1724 1396 1183 1179 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2687 2774 4300 4487 1700 Size: 110 877 87 1526 187 2869 Fragments arranged by size: 2869 1526 877 187 110 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 2893 2948 1925 Size: 968 55 4633 Fragments arranged by size: 4633 968 55 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 2429 2878 794 Size: 1635 449 3572 Fragments arranged by size: 3572 1635 449 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstXI CCAn_nnnn'nTGG Cuts at: 2213 2936 2213 Size: 723 4933 BstYI r'GATC_y Cuts at: 1972 2532 2986 3353 3738 3755 5207 5293 5305 Size: 560 454 367 385 17 1452 86 12 Cuts at: 5305 5651 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 560 454 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2915 2915 Size: 5656 BtgI C'CryG_G Cuts at: 513 1065 2004 2887 513 Size: 552 939 883 3282 Fragments arranged by size: 3282 939 883 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2224 2262 2524 3172 3966 3986 2224 Size: 38 262 648 794 20 3894 Fragments arranged by size: 3894 794 648 262 38 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DraIII CAC_nnn'GTG Cuts at: 2395 2395 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4663 809 Size: 507 1305 2042 1802 Fragments arranged by size: 2042 1802 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2772 3103 Size: 54 1425 50 275 18 270 672 331 Cuts at: 3103 3992 5621 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 672 331 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3586 1353 Size: 2233 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2618 3719 4316 5103 5371 1239 Size: 393 405 581 1101 597 787 268 1524 Fragments arranged by size: 1524 1101 787 597 581 405 393 268 EcoO109I rG'GnC_Cy Cuts at: 2217 2325 2473 2660 2734 2735 2910 2911 2217 Size: 108 148 187 74 1 175 1 4962 Fragments arranged by size: 4962 187 175 148 108 74 1 1 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 2230 2844 4162 2230 Size: 614 1318 3724 Fragments arranged by size: 3724 1318 614 HaeII r_GCGC'y Cuts at: 1678 2024 2452 2763 4803 1678 Size: 346 428 311 2040 2531 Fragments arranged by size: 2531 2040 428 346 311 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2082 3000 3246 669 Size: 988 281 144 918 246 3079 Fragments arranged by size: 3079 988 918 281 246 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KasI G'GCGC_C Cuts at: 2759 2759 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3973 4132 518 Size: 648 2448 359 159 2042 Fragments arranged by size: 2448 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2253 2335 2787 2889 3072 3749 4897 Size: 537 110 82 452 102 183 677 1148 Cuts at: 4897 5142 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 537 452 245 183 110 102 82 NaeI GCC'GGC Cuts at: 2689 2776 2689 Size: 87 5569 NarI GG'CG_CC Cuts at: 2760 2760 Size: 5656 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NgoMIV G'CCGG_C Cuts at: 2687 2774 2687 Size: 87 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2680 3014 4559 1132 Size: 97 1451 334 1545 2229 Fragments arranged by size: 2229 1545 1451 334 97 PasI CC'CwG_GG Cuts at: 1348 2032 1348 Size: 684 4972 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2126 2253 2365 1108 Size: 1018 127 112 4399 Fragments arranged by size: 4399 1018 127 112 PfoI T'CCnGG_A Cuts at: 2402 2402 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2333 2365 3725 3757 5238 5270 2333 Size: 32 1360 32 1481 32 2719 Fragments arranged by size: 2719 1481 1360 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2217 2473 2660 2217 Size: 256 187 5213 Fragments arranged by size: 5213 256 187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PspOMI G'GGCC_C Cuts at: 2734 2910 2734 Size: 176 5480 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2285 2317 2285 Size: 32 5624 PstI C_TGCA'G Cuts at: 830 2592 2630 3008 830 Size: 1762 38 378 3478 Fragments arranged by size: 3478 1762 378 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2143 2335 2787 2143 Size: 192 452 5012 Fragments arranged by size: 5012 452 192 SacII CC_GC'GG Cuts at: 2890 2890 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2080 2998 1936 Size: 144 918 4594 Fragments arranged by size: 4594 918 144 SanDI GG'GwC_CC Cuts at: 2217 2660 2217 Size: 443 5213 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2588 2626 3004 4139 4820 5011 826 Size: 219 1543 38 378 1135 681 191 1471 Fragments arranged by size: 1543 1471 1135 681 378 219 191 38 SfoI GGC'GCC Cuts at: 2761 2761 Size: 5656 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2821 2983 1872 Size: 949 162 4545 Fragments arranged by size: 4545 949 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 3760 4661 4923 820 Size: 197 926 501 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 501 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2004 3095 513 Size: 552 939 1091 3074 Fragments arranged by size: 3074 1091 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2245 2343 2357 2368 2537 2845 Size: 1255 444 221 98 14 11 169 308 Cuts at: 2845 3683 3700 3853 4038 4483 325 Size: 838 17 153 185 445 1498 Fragments arranged by size: 1498 1255 838 445 444 308 221 185 169 153 98 17 14 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 3902 5614 Size: 274 80 33 51 1443 660 1265 1712 Cuts at: 5614 96 Size: 138 Fragments arranged by size: 1712 1443 1265 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2398 2430 2666 2698 5616 5648 2398 Size: 32 236 32 2918 32 2406 Fragments arranged by size: 2918 2406 236 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 2444 1943 Size: 501 5155 XmaI C'CCGG_G Cuts at: 1870 2819 2981 1870 Size: 949 162 4545 Fragments arranged by size: 4545 949 162 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI