(Linear) (Six-base) MAPSORT of: KIEE5441.seq Check: 9960 from: 1 to: 951 KIEE5441.orf With 182 enzymes: SgfI * January 29, 2014 12:00 .. AccI GT'mk_AC Cuts at: 0 76 821 951 Size: 76 745 130 Fragments arranged by size: 745 130 76 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 951 Size: 613 338 AfeI AGC'GCT Cuts at: 0 445 951 Size: 445 506 AlwNI CAG_nnn'CTG Cuts at: 0 138 248 582 591 951 Size: 138 110 334 9 360 Fragments arranged by size: 360 334 138 110 9 ApaI G_GGCC'C Cuts at: 0 733 909 951 Size: 733 176 42 Fragments arranged by size: 733 176 42 AvaI C'yCGr_G Cuts at: 0 439 814 951 Size: 439 375 137 Fragments arranged by size: 439 375 137 BanI G'GyrC_C Cuts at: 0 46 409 754 915 951 Size: 46 363 345 161 36 Fragments arranged by size: 363 345 161 46 36 BanII G_rGCy'C Cuts at: 0 383 733 909 931 951 Size: 383 350 176 22 20 Fragments arranged by size: 383 350 176 22 20 BbeI G_GCGC'C Cuts at: 0 758 951 Size: 758 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 778 812 951 Size: 778 34 139 Fragments arranged by size: 778 139 34 BciVI GTATCCnnnnn_n' Cuts at: 0 95 951 Size: 95 856 BclI T'GATC_A Cuts at: 0 254 951 Size: 254 697 BglI GCCn_nnn'nGGC Cuts at: 0 913 951 Size: 913 38 Bme1580I G_kGCm'C Cuts at: 0 733 909 918 951 Size: 733 176 9 33 Fragments arranged by size: 733 176 33 9 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 951 Size: 32 919 Bpu10I CC'TnA_GC Cuts at: 0 893 951 Size: 893 58 BsaHI Gr'CG_yC Cuts at: 0 73 755 951 Size: 73 682 196 Fragments arranged by size: 682 196 73 BseYI C'CCAG_C Cuts at: 0 21 114 131 248 315 330 536 782 Size: 21 93 17 117 67 15 206 246 Cuts at: 782 951 Size: 169 Fragments arranged by size: 246 206 169 117 93 67 21 17 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 895 951 Size: 437 458 56 Fragments arranged by size: 458 437 56 BsmBI CGTCTCn'nnnn_ Cuts at: 0 442 951 Size: 442 509 Bsp1286I G_dGCh'C Cuts at: 0 383 733 909 918 931 951 Size: 383 350 176 9 13 20 Fragments arranged by size: 383 350 176 20 13 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 217 951 Size: 217 734 BsrBI CCG'CTC Cuts at: 0 465 702 845 951 Size: 465 237 143 106 Fragments arranged by size: 465 237 143 106 BsrDI GCAATG_nn' Cuts at: 0 422 951 Size: 422 529 BsrFI r'CCGG_y Cuts at: 0 682 769 951 Size: 682 87 182 Fragments arranged by size: 682 182 87 BssHII G'CGCG_C Cuts at: 0 888 943 951 Size: 888 55 8 Fragments arranged by size: 888 55 8 BstAPI GCAn_nnn'nTGC Cuts at: 0 424 873 951 Size: 424 449 78 Fragments arranged by size: 449 424 78 BstXI CCAn_nnnn'nTGG Cuts at: 0 208 931 951 Size: 208 723 20 Fragments arranged by size: 723 208 20 BstYI r'GATC_y Cuts at: 0 527 951 Size: 527 424 Bsu36I CC'TnA_GG Cuts at: 0 910 951 Size: 910 41 BtgI C'CryG_G Cuts at: 0 882 951 Size: 882 69 BtsI GCAGTG_nn' Cuts at: 0 219 257 519 951 Size: 219 38 262 432 Fragments arranged by size: 432 262 219 38 DraIII CAC_nnn'GTG Cuts at: 0 390 951 Size: 390 561 DrdI GACnn_nn'nnGTC Cuts at: 0 616 951 Size: 616 335 EaeI y'GGCC_r Cuts at: 0 95 767 951 Size: 95 672 184 Fragments arranged by size: 672 184 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 613 951 Size: 32 581 338 Fragments arranged by size: 581 338 32 EcoO109I rG'GnC_Cy Cuts at: 0 212 320 468 655 729 730 905 906 Size: 212 108 148 187 74 1 175 1 Cuts at: 906 951 Size: 45 Fragments arranged by size: 212 187 175 148 108 74 45 1 1 FspI TGC'GCA Cuts at: 0 225 839 951 Size: 225 614 112 Fragments arranged by size: 614 225 112 HaeII r_GCGC'y Cuts at: 0 19 447 758 951 Size: 19 428 311 193 Fragments arranged by size: 428 311 193 19 HincII GTy'rAC Cuts at: 0 77 951 Size: 77 874 KasI G'GCGC_C Cuts at: 0 754 951 Size: 754 197 MspA1I CmG'CkG Cuts at: 0 138 248 330 782 884 951 Size: 138 110 82 452 102 67 Fragments arranged by size: 452 138 110 102 82 67 NaeI GCC'GGC Cuts at: 0 684 771 951 Size: 684 87 180 Fragments arranged by size: 684 180 87 NarI GG'CG_CC Cuts at: 0 755 951 Size: 755 196 NgoMIV G'CCGG_C Cuts at: 0 682 769 951 Size: 682 87 182 Fragments arranged by size: 682 182 87 NspI r_CATG'y Cuts at: 0 675 951 Size: 675 276 PasI CC'CwG_GG Cuts at: 0 27 951 Size: 27 924 PflMI CCAn_nnn'nTGG Cuts at: 0 121 248 360 951 Size: 121 127 112 591 Fragments arranged by size: 591 127 121 112 PfoI T'CCnGG_A Cuts at: 0 397 951 Size: 397 554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 328 360 951 Size: 328 32 591 Fragments arranged by size: 591 328 32 PpuMI rG'GwC_Cy Cuts at: 0 212 468 655 951 Size: 212 256 187 296 Fragments arranged by size: 296 256 212 187 PspOMI G'GGCC_C Cuts at: 0 729 905 951 Size: 729 176 46 Fragments arranged by size: 729 176 46 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 280 312 951 Size: 280 32 639 Fragments arranged by size: 639 280 32 PstI C_TGCA'G Cuts at: 0 587 625 951 Size: 587 38 326 Fragments arranged by size: 587 326 38 PvuII CAG'CTG Cuts at: 0 138 330 782 951 Size: 138 192 452 169 Fragments arranged by size: 452 192 169 138 SacII CC_GC'GG Cuts at: 0 885 951 Size: 885 66 SalI G'TCGA_C Cuts at: 0 75 951 Size: 75 876 SanDI GG'GwC_CC Cuts at: 0 212 655 951 Size: 212 443 296 Fragments arranged by size: 443 296 212 SfcI C'TryA_G Cuts at: 0 583 621 951 Size: 583 38 330 Fragments arranged by size: 583 330 38 SfoI GGC'GCC Cuts at: 0 756 951 Size: 756 195 SmaI CCC'GGG Cuts at: 0 816 951 Size: 816 135 SmlI C'TyrA_G Cuts at: 0 439 951 Size: 439 512 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 240 338 352 363 532 840 951 Size: 19 221 98 14 11 169 308 111 Fragments arranged by size: 308 221 169 111 98 19 14 11 TatI w'GTAC_w Cuts at: 0 632 951 Size: 632 319 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 393 425 661 693 951 Size: 393 32 236 32 258 Fragments arranged by size: 393 258 236 32 32 XhoI C'TCGA_G Cuts at: 0 439 951 Size: 439 512 XmaI C'CCGG_G Cuts at: 0 814 951 Size: 814 137 Enzymes that do cut: AccI AcuI AfeI AlwNI ApaI AvaI BanI BanII BbeI BcgI BciVI BclI BglI Bme1580I BpmI Bpu10I BsaHI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI Eco57MI EcoO109I FspI HaeII HincII KasI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacII SalI SanDI SfcI SfoI SmaI SmlI TaqII TatI TstI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI XmnI ZraI