################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AocI ApaI ApaLI AscI Asp718I AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 351 CGCG CG^CG AcsI 379 GAATTT [GA]^AATT[CT] AhdI 140 GACTCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 229 GATCC GGATC(4/5) AlwNI 178 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 85 AGCGCT AGC^GCT ApoI 379 GAATTT [GA]^AATT[CT] AseI 399 ATTAAT AT^TAAT AsnI 399 ATTAAT AT^TAAT Asp700I 198 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 140 GACTCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 35 CCCGGG C^[CT]CG[GA]G BbsI 586 GAAGAC GAAGAC(2/6) BpuAI 586 GAAGAC GAAGAC(2/6) BsaI 281 GAGACC GGTCTC(1/5) BsgI 625 CTGCAC GTGCAG(16/14) BsiWI 638 CGTACG C^GTACG BsmFI 191 GGGAC GGGAC(10/14) BsoBI 35 CCCGGG C^[CT]CG[GA]G BspMI 450 GCAGGT ACCTGC(4/8) BsrFI 30 GCCGGC [GA]^CCGG[CT] BstUI 351 CGCG CG^CG BstXI 108 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 30 GCCGGC [GA]^CCGG[CT] DpnII 229 GATC ^GATC DraII 191 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 647 CGGCCA [CT]^GGCC[GA] Eam1105I 140 GACTCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 85 AGCGCT AGC^GCT EcoNI 348 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 191 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 617 CCAAGG C^C[AT][AT]GG FauI 52 GCGGG CCCGC(4/6) MaeII 146 ACGT A^CGT MboI 229 GATC ^GATC MboII 586 GAAGA GAAGA(8/7) MslI 504 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 78 CCGCTG C[AC]G^C[GT]G MvnI 351 CGCG CG^CG NaeI 30 GCCGGC GCC^GGC NdeII 229 GATC ^GATC NgoMIV 30 GCCGGC G^CCGGC NspI 465 GCATGT [GA]CATG^[CT] PpuMI 191 GGGACCC [GA]G^G[AT]CC[CT] PshBI 399 ATTAAT AT^TAAT Sau3AI 229 GATC ^GATC SmaI 35 CCCGGG CCC^GGG SmlI 693 CTCAAG C^T[CT][GA]AG StyI 617 CCAAGG C^C[AT][AT]GG TfiI 9 GAATC G^A[AT]TC XmaCI 35 CCCGGG C^CCGGG XmaI 35 CCCGGG C^CCGGG XmnI 198 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 97 GGCGCC G[GA]^CG[CT]C 262 GGCGCC AspHI 19 GAGCTC G[AT]GC[AT]^C 499 GAGCTC AvaII 135 GGTCC G^G[AT]CC 192 GGACC BbeI 97 GGCGCC GGCGC^C 262 GGCGCC BsaHI 97 GGCGCC G[GA]^CG[CT]C 262 GGCGCC BsiHKAI 19 GAGCTC G[AT]GC[AT]^C 499 GAGCTC BstF5I 252 GGATG GGATG(2/0) 698 GGATG DdeI 243 CTCAG C^T[ACGT]AG 316 CTGAG FokI 252 GGATG GGATG(9/13) 698 GGATG Hin1I 97 GGCGCC G[GA]^CG[CT]C 262 GGCGCC KasI 97 GGCGCC G^GCGCC 262 GGCGCC NarI 97 GGCGCC GG^CGCC 262 GGCGCC NlaIII 466 CATG CATG^ 522 CATG PleI 140 GACTC GAGTC(4/5) 241 GACTC PstI 81 CTGCAG CTGCA^G 481 CTGCAG SacI 19 GAGCTC GAGCT^C 499 GAGCTC SfaNI 44 GCATC GCATC(5/9) 557 GCATC SfcI 81 CTGCAG C^T[GA][CT]AG 481 CTGCAG SfoI 97 GGCGCC GGC^GCC 262 GGCGCC TaqI 275 TCGA T^CGA 542 TCGA Tsp509I 380 AATT ^AATT 423 AATT TspRI 13 CACTG CA[GC]TG[ACGT][ACGT]^ 484 CAGTG TthHB8I 275 TCGA T^CGA 542 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MnlI MseI MspI MvaI MwoI NciI NlaIV RsaI Sau96I ScrFI Tru9I TseI