(Circular) (Six-base) MAPSORT of: pFN21AE4026.seq Check: 3743 from: 1 to: 5407 pFN21AE4026 5407 bp With 182 enzymes: SgfI * March 28, 2012 13:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2446 2446 Size: 5407 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3470 4854 5122 1632 Size: 1838 1384 268 1917 Fragments arranged by size: 1917 1838 1384 268 AfeI AGC'GCT Cuts at: 2092 2092 Size: 5407 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 2150 4136 2150 Size: 1986 3421 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5407 AlwNI CAG_nnn'CTG Cuts at: 2188 3150 4722 2188 Size: 962 1572 2873 Fragments arranged by size: 2873 1572 962 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2384 2716 2942 3094 5203 1493 Size: 891 332 226 152 2109 1697 Fragments arranged by size: 2109 1697 891 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 2405 3961 160 Size: 872 1373 1556 1606 Fragments arranged by size: 1606 1556 1373 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2732 1870 Size: 73 97 692 4545 Fragments arranged by size: 4545 692 97 73 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2159 2267 2458 2728 Size: 325 217 627 315 57 108 191 270 Cuts at: 2728 4183 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 315 270 217 191 108 57 BanII G_rGCy'C Cuts at: 1391 2028 2100 2508 2664 2726 1391 Size: 637 72 408 156 62 4072 Fragments arranged by size: 4072 637 408 156 72 62 BbeI G_GCGC'C Cuts at: 2106 2271 2106 Size: 165 5242 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2598 928 Size: 971 699 3737 Fragments arranged by size: 3737 971 699 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 1903 Size: 34 1661 34 3678 Fragments arranged by size: 3678 1661 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4018 136 Size: 107 122 71 631 605 2346 1525 Fragments arranged by size: 2346 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2824 2824 Size: 5407 Bme1580I G_kGCm'C Cuts at: 1790 3468 4624 1790 Size: 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4096 442 Size: 416 53 83 399 24 1721 958 1753 Fragments arranged by size: 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4067 1239 Size: 2828 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2683 3532 4397 4695 2683 Size: 849 865 298 3395 Fragments arranged by size: 3395 865 849 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2280 4070 882 Size: 617 781 1790 2219 Fragments arranged by size: 2219 1790 781 617 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2268 3214 3596 Size: 53 83 186 936 570 165 946 382 Cuts at: 3596 4231 275 Size: 635 1451 Fragments arranged by size: 1451 946 936 635 570 382 186 165 83 53 BsaWI w'CCGG_w Cuts at: 3840 4238 4512 4659 5070 3840 Size: 398 274 147 411 4177 Fragments arranged by size: 4177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5182 Fragments arranged by size: 5182 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2516 2560 1186 Size: 1327 3 44 4033 Fragments arranged by size: 4033 1327 44 3 BseYI C'CCAG_C Cuts at: 2062 2093 2112 2663 4610 5106 2062 Size: 31 19 551 1947 496 2363 Fragments arranged by size: 2363 1947 551 496 31 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2615 1163 Size: 573 879 3955 Fragments arranged by size: 3955 879 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3618 4646 1540 Size: 275 186 856 761 1028 2301 Fragments arranged by size: 2301 1028 856 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2508 3468 3553 4624 2028 Size: 480 960 85 1071 2811 Fragments arranged by size: 2811 1071 960 480 85 BsiWI C'GTAC_G Cuts at: 2643 2643 Size: 5407 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2100 2508 2664 2726 3468 3553 Size: 399 238 72 408 156 62 742 85 Cuts at: 3553 4624 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 238 156 85 72 62 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 2762 844 Size: 1602 316 3489 Fragments arranged by size: 3489 1602 316 BsrBI CCG'CTC Cuts at: 1394 1823 3294 1394 Size: 429 1471 3507 Fragments arranged by size: 3507 1471 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 4051 4238 1700 Size: 110 225 2016 187 2869 Fragments arranged by size: 2869 2016 225 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5407 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5407 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstXI CCAn_nnnn'nTGG Cuts at: 2120 2120 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402 Size: 765 367 385 17 1452 86 12 346 Cuts at: 5402 1972 Size: 1977 Fragments arranged by size: 1977 1452 765 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3916 Fragments arranged by size: 3916 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2436 487 Size: 683 56 1210 3458 Fragments arranged by size: 3458 1210 683 56 BtsI GCAGTG_nn' Cuts at: 2017 2495 2923 3717 3737 2017 Size: 478 428 794 20 3687 Fragments arranged by size: 3687 794 478 428 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2281 2316 529 Size: 35 1717 35 3620 Fragments arranged by size: 3620 1717 35 35 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2652 2854 3743 Size: 54 1425 50 275 18 822 202 889 Cuts at: 3743 5372 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 822 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2854 1537 Size: 275 1042 4090 Fragments arranged by size: 4090 1042 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 1353 Size: 1984 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3994 4368 4514 387 Size: 1064 138 435 1970 374 146 1280 Fragments arranged by size: 1970 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 2506 2026 Size: 480 4927 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3470 4067 4854 5122 1239 Size: 393 1838 597 787 268 1524 Fragments arranged by size: 1838 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2357 Size: 5407 EcoO109I rG'GnC_Cy Cuts at: 2197 2197 Size: 5407 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2094 2106 2119 2271 4554 1678 Size: 416 12 13 152 2283 2531 Fragments arranged by size: 2531 2283 416 152 13 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2136 2168 2189 Size: 32 135 32 443 32 89 32 21 Cuts at: 2189 2221 2231 2263 4048 4080 4122 4154 1373 Size: 32 10 32 1785 32 42 32 2626 Fragments arranged by size: 2626 1785 443 135 89 42 32 32 32 32 32 32 32 32 21 10 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2102 2267 2102 Size: 165 5242 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2167 2352 2556 4521 4705 1305 Size: 862 185 204 1965 184 2007 Fragments arranged by size: 2007 1965 862 204 185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3365 3724 3883 518 Size: 648 1347 852 359 159 2042 Fragments arranged by size: 2042 1347 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2823 3500 4648 4893 1606 Size: 479 738 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 479 245 NaeI GCC'GGC Cuts at: 2037 2037 Size: 5407 NarI GG'CG_CC Cuts at: 2103 2268 2103 Size: 165 5242 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3916 Fragments arranged by size: 3916 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NgoMIV G'CCGG_C Cuts at: 2035 2035 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2474 2765 4310 1132 Size: 97 1245 291 1545 2229 Fragments arranged by size: 2229 1545 1245 291 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5407 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2197 Size: 5407 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PstI C_TGCA'G Cuts at: 830 2090 2490 2759 830 Size: 1260 400 269 3478 Fragments arranged by size: 3478 1260 400 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 SacI G_AGCT'C Cuts at: 2028 2508 2028 Size: 480 4927 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SanDI GG'GwC_CC Cuts at: 2197 2197 Size: 5407 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2086 2486 2755 3890 4571 4762 826 Size: 219 1041 400 269 1135 681 191 1471 Fragments arranged by size: 1471 1135 1041 681 400 269 219 191 SfoI GGC'GCC Cuts at: 2104 2269 2104 Size: 165 5242 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2042 2734 1872 Size: 170 692 4545 Fragments arranged by size: 4545 692 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2698 3511 4412 4674 820 Size: 197 926 755 813 901 262 1553 Fragments arranged by size: 1553 926 901 813 755 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5407 StyI C'CwwG_G Cuts at: 513 1065 2004 2622 2846 513 Size: 552 939 618 224 3074 Fragments arranged by size: 3074 939 618 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3434 3451 3604 3789 4234 325 Size: 1255 1854 17 153 185 445 1498 Fragments arranged by size: 1854 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3653 5365 96 Size: 274 80 33 51 1443 1676 1712 138 Fragments arranged by size: 1712 1676 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2040 2732 1870 Size: 170 692 4545 Fragments arranged by size: 4545 692 170 XmnI GAAnn'nnTTC Cuts at: 2207 3536 2207 Size: 1329 4078 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI