(Circular) (Six-base) MAPSORT of: pFN21AE3269.seq Check: 3918 from: 1 to: 6715 pFN21AE3269 6715 bp With 182 enzymes: SgfI * February 10, 2017 15:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3766 3766 Size: 6715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2076 3021 3677 4525 Size: 53 83 186 936 540 945 656 848 Cuts at: 4525 278 Size: 2468 Fragments arranged by size: 2468 945 936 848 656 540 186 83 53 AccI GT'mk_AC Cuts at: 1937 3216 3690 4058 1937 Size: 1279 474 368 4594 Fragments arranged by size: 4594 1279 474 368 Acc65I G'GTAC_C Cuts at: 4036 4036 Size: 6715 AclI AA'CG_TT Cuts at: 1563 4842 5215 1563 Size: 3279 373 3063 Fragments arranged by size: 3279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2885 3223 3466 3515 3554 3838 4778 6162 Size: 1253 338 243 49 39 284 940 1384 Cuts at: 6162 6430 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1253 940 338 284 268 243 49 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6518 AflIII A'CryG_T Cuts at: 1203 2438 2448 3278 3750 5614 6591 1203 Size: 1235 10 830 472 1864 977 1327 Fragments arranged by size: 1864 1327 1235 977 830 472 10 AgeI A'CCGG_T Cuts at: 3870 5546 3870 Size: 1676 5039 AhdI GACnn_n'nnGTC Cuts at: 3097 3763 5444 3097 Size: 666 1681 4368 Fragments arranged by size: 4368 1681 666 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2327 1184 Size: 1143 5572 AlwNI CAG_nnn'CTG Cuts at: 3409 3885 3919 3983 4458 6030 3409 Size: 476 34 64 475 1572 4094 Fragments arranged by size: 4094 1572 476 475 64 34 ApaI G_GGCC'C Cuts at: 2052 3197 3436 3580 2052 Size: 1145 239 144 5187 Fragments arranged by size: 5187 1145 239 144 ApaLI G'TGCA_C Cuts at: 2800 3451 4772 5928 2800 Size: 651 1321 1156 3587 Fragments arranged by size: 3587 1321 1156 651 ApoI r'AATT_y Cuts at: 1493 4024 4250 4402 6511 1493 Size: 2531 226 152 2109 1697 Fragments arranged by size: 2531 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5269 160 Size: 872 4237 1606 Fragments arranged by size: 4237 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 AvaI C'yCGr_G Cuts at: 1870 1943 2333 2625 3960 4040 1870 Size: 73 390 292 1335 80 4545 Fragments arranged by size: 4545 1335 390 292 80 73 AvrII C'CTAG_G Cuts at: 3133 3133 Size: 6715 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3265 3298 3265 Size: 33 6682 BamHI G'GATC_C Cuts at: 4045 4412 4045 Size: 367 6348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2631 3728 3803 4036 5491 Size: 325 217 627 844 1097 75 233 1455 Cuts at: 5491 618 Size: 1842 Fragments arranged by size: 1842 1455 1097 844 627 325 233 217 75 BanII G_rGCy'C Cuts at: 1391 2012 2052 2556 3009 3197 3436 3552 3580 Size: 621 40 504 453 188 239 116 28 Cuts at: 3580 4034 1391 Size: 454 4072 Fragments arranged by size: 4072 621 504 454 453 239 188 116 40 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2407 3308 3844 928 Size: 971 508 901 536 3799 Fragments arranged by size: 3799 971 901 536 508 BbvCI CC'TCA_GC Cuts at: 3150 3150 Size: 6715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2791 2825 4906 4940 1903 Size: 34 854 34 2081 34 3678 Fragments arranged by size: 3678 2081 854 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5817 4607 Size: 1210 5505 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2574 3192 3321 Size: 107 122 71 631 605 902 618 129 Cuts at: 3321 3584 3801 5326 136 Size: 263 217 1525 1525 Fragments arranged by size: 1525 1525 902 631 618 605 263 217 129 122 107 71 BglII A'GATC_T Cuts at: 3156 3630 6710 3156 Size: 474 3080 3161 Fragments arranged by size: 3161 3080 474 BlpI GC'TnA_GC Cuts at: 2651 3044 4132 2651 Size: 393 1088 5234 Fragments arranged by size: 5234 1088 393 Bme1580I G_kGCm'C Cuts at: 1790 2026 2052 2448 2636 2804 3197 3436 3455 Size: 236 26 396 188 168 393 239 19 Cuts at: 3455 3527 3580 3806 4776 5932 1790 Size: 72 53 226 970 1156 2573 Fragments arranged by size: 2573 1156 970 396 393 239 236 226 188 168 72 53 26 19 BmgBI CAC'GTC Cuts at: 1318 3664 1318 Size: 2346 4369 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3577 4446 5404 Size: 416 53 83 399 24 2160 869 958 Cuts at: 5404 442 Size: 1753 Fragments arranged by size: 2160 1753 958 869 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6715 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2395 2395 Size: 6715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2765 2861 3023 3329 3907 5375 1239 Size: 937 589 96 162 306 578 1468 2579 Fragments arranged by size: 2579 1468 937 589 578 306 162 96 Bpu10I CC'TnA_GC Cuts at: 2011 2732 3150 2011 Size: 721 418 5576 Fragments arranged by size: 5576 721 418 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2741 2993 4840 5705 6003 2741 Size: 252 1847 865 298 3453 Fragments arranged by size: 3453 1847 865 298 252 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2825 3466 5378 882 Size: 617 1326 641 1912 2219 Fragments arranged by size: 2219 1912 1326 641 617 BsaAI yAC'GTr Cuts at: 493 1204 2439 2911 3751 3799 4506 493 Size: 711 1235 472 840 48 707 2702 Fragments arranged by size: 2702 1235 840 711 707 472 48 BsaBI GATnn'nnATC Cuts at: 6709 6709 Size: 6715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 3018 3674 3833 Size: 53 83 186 936 540 945 656 159 Cuts at: 3833 4522 4904 5539 275 Size: 689 382 635 1451 Fragments arranged by size: 1451 945 936 689 656 635 540 382 186 159 83 53 BsaWI w'CCGG_w Cuts at: 3870 5148 5546 5820 5967 6378 3870 Size: 1278 398 274 147 411 4207 Fragments arranged by size: 4207 1278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 2996 3026 3596 Size: 30 165 30 621 30 942 30 570 Cuts at: 3596 3626 1178 Size: 30 4267 Fragments arranged by size: 4267 942 621 570 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2176 2179 2237 2351 2354 2357 2360 3238 Size: 990 3 58 114 3 3 3 878 Cuts at: 3238 3454 1186 Size: 216 4447 Fragments arranged by size: 4447 990 878 216 114 58 3 3 3 3 BseYI C'CCAG_C Cuts at: 2051 2714 2959 5918 6414 2051 Size: 663 245 2959 496 2352 Fragments arranged by size: 2959 2352 663 496 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 3364 3892 1163 Size: 573 537 1091 528 3986 Fragments arranged by size: 3986 1091 573 537 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3871 4165 4926 5954 1540 Size: 275 186 1870 294 761 1028 2301 Fragments arranged by size: 2301 1870 1028 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2556 2772 2804 3009 3455 3480 3552 4776 Size: 544 216 32 205 446 25 72 1224 Cuts at: 4776 4861 5932 2012 Size: 85 1071 2795 Fragments arranged by size: 2795 1224 1071 544 446 216 205 85 72 32 25 BsmI GAATG_Cn' Cuts at: 4226 4319 4226 Size: 93 6622 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2026 2052 2448 2556 2636 2772 Size: 399 222 14 26 396 108 80 136 Cuts at: 2772 2804 3009 3197 3436 3455 3480 3527 3552 Size: 32 205 188 239 19 25 47 25 Cuts at: 3552 3580 3806 4034 4776 4861 5932 1391 Size: 28 226 228 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 239 228 226 222 205 188 136 108 85 80 47 32 28 26 25 25 19 14 BspEI T'CCGG_A Cuts at: 6378 6378 Size: 6715 BspHI T'CATG_A Cuts at: 4604 6334 4604 Size: 1730 4985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3240 3417 3766 4070 844 Size: 2396 177 349 304 3489 Fragments arranged by size: 3489 2396 349 304 177 BsrBI CCG'CTC Cuts at: 1394 1823 2259 2283 4602 1394 Size: 429 436 24 2319 3507 Fragments arranged by size: 3507 2319 436 429 24 BsrDI GCAATG_nn' Cuts at: 65 1248 3696 5210 5384 65 Size: 1183 2448 1514 174 1396 Fragments arranged by size: 2448 1514 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2568 3870 5359 5546 1700 Size: 110 758 1302 1489 187 2869 Fragments arranged by size: 2869 1489 1302 758 187 110 BsrGI T'GTAC_A Cuts at: 96 3603 96 Size: 3507 3208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6715 BssSI C'ACGA_G Cuts at: 2669 2900 4775 5787 2669 Size: 231 1875 1012 3597 Fragments arranged by size: 3597 1875 1012 231 BstAPI GCAn_nnn'nTGC Cuts at: 794 3919 794 Size: 3125 3590 BstBI TT'CG_AA Cuts at: 5519 5519 Size: 6715 BstEII G'GTnAC_C Cuts at: 1184 3227 1184 Size: 2043 4672 BstYI r'GATC_y Cuts at: 1972 3156 3630 4045 4412 4797 4814 6266 6352 Size: 1184 474 415 367 385 17 1452 86 Cuts at: 6352 6364 6710 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1184 474 415 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3878 3878 Size: 6715 BtgI C'CryG_G Cuts at: 513 1065 2213 2490 2511 3222 513 Size: 552 1148 277 21 711 4006 Fragments arranged by size: 4006 1148 711 552 277 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2512 487 Size: 683 56 1286 4690 Fragments arranged by size: 4690 1286 683 56 BtsI GCAGTG_nn' Cuts at: 2170 2327 2563 3943 4231 5025 5045 2170 Size: 157 236 1380 288 794 20 3840 Fragments arranged by size: 3840 1380 794 288 236 157 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6715 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6680 DraI TTT'AAA Cuts at: 4019 4019 Size: 6715 DraIII CAC_nnn'GTG Cuts at: 2439 3475 3571 2439 Size: 1036 96 5583 Fragments arranged by size: 5583 1036 96 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5722 809 Size: 507 4406 1802 Fragments arranged by size: 4406 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2535 4162 5051 Size: 54 1425 50 275 18 705 1627 889 Cuts at: 5051 6680 8 Size: 1629 43 Fragments arranged by size: 1629 1627 1425 889 705 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4162 1537 Size: 275 2350 4090 Fragments arranged by size: 4090 2350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2561 2990 3242 3485 3977 4645 1353 Size: 1208 429 252 243 492 668 3423 Fragments arranged by size: 3423 1208 668 492 429 252 243 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 5302 5676 5822 387 Size: 1064 138 668 3045 374 146 1280 Fragments arranged by size: 3045 1280 1064 668 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2554 3007 3550 2010 Size: 544 453 543 5175 Fragments arranged by size: 5175 544 543 453 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2765 2861 2885 3023 3223 3329 Size: 393 544 589 96 24 138 200 106 Cuts at: 3329 3466 3515 3554 3838 3907 4778 5375 6162 Size: 137 49 39 284 69 871 597 787 Cuts at: 6162 6430 1239 Size: 268 1524 Fragments arranged by size: 1524 871 787 597 589 544 393 284 268 200 138 137 106 96 69 49 39 24 EcoNI CCTnn'n_nnAGG Cuts at: 3413 3413 Size: 6715 EcoO109I rG'GnC_Cy Cuts at: 2048 2860 3052 3174 3193 3194 3577 2048 Size: 812 192 122 19 1 383 5186 Fragments arranged by size: 5186 812 383 192 122 19 1 EcoRI G'AATT_C Cuts at: 4024 4402 4024 Size: 378 6337 FspI TGC'GCA Cuts at: 5221 5221 Size: 6715 HaeII r_GCGC'y Cuts at: 1678 2658 5862 1678 Size: 980 3204 2531 Fragments arranged by size: 3204 2531 980 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3083 3115 3749 3781 5356 Size: 32 135 32 1511 32 634 32 1575 Cuts at: 5356 5388 5430 5462 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1575 1511 634 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4059 4305 669 Size: 988 281 2121 246 3079 Fragments arranged by size: 3079 2121 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6715 HpaI GTT'AAC Cuts at: 4305 4305 Size: 6715 KpnI G_GTAC'C Cuts at: 4040 4040 Size: 6715 MfeI C'AATT_G Cuts at: 4314 4314 Size: 6715 MluI A'CGCG_T Cuts at: 6591 6591 Size: 6715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 5829 6013 1305 Size: 981 3543 184 2007 Fragments arranged by size: 3543 2007 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4893 Fragments arranged by size: 4893 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 3266 3389 3701 3749 4673 5032 Size: 648 1365 735 123 312 48 924 359 Cuts at: 5032 5191 518 Size: 159 2042 Fragments arranged by size: 2042 1365 924 735 648 359 312 159 123 48 MspA1I CmG'CkG Cuts at: 1606 2215 2595 2679 2718 2728 3366 3486 3609 Size: 609 380 84 39 10 638 120 123 Cuts at: 3609 4131 4808 5956 6201 1606 Size: 522 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 638 609 522 380 245 123 120 84 39 10 NaeI GCC'GGC Cuts at: 2570 2570 Size: 6715 NcoI C'CATG_G Cuts at: 513 1065 3222 513 Size: 552 2157 4006 Fragments arranged by size: 4006 2157 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6715 NgoMIV G'CCGG_C Cuts at: 2568 2568 Size: 6715 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6715 NotI GC'GGCC_GC Cuts at: 4162 4162 Size: 6715 NspI r_CATG'y Cuts at: 1132 1229 2236 2308 3282 4073 5618 1132 Size: 97 1007 72 974 791 1545 2229 Fragments arranged by size: 2229 1545 1007 974 791 97 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6715 PciI A'CATG_T Cuts at: 3278 5614 3278 Size: 2336 4379 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6715 PfoI T'CCnGG_A Cuts at: 3021 3021 Size: 6715 PmeI GTTT'AAAC Cuts at: 4019 4019 Size: 6715 PmlI CAC'GTG Cuts at: 2439 2911 3751 3799 2439 Size: 472 840 48 5355 Fragments arranged by size: 5355 840 472 48 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4784 4816 6297 6329 4784 Size: 32 1481 32 5170 Fragments arranged by size: 5170 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3052 3174 3052 Size: 122 6593 PshAI GACnn'nnGTC Cuts at: 1613 3848 1613 Size: 2235 4480 PsiI TTA'TAA Cuts at: 4285 4285 Size: 6715 PspOMI G'GGCC_C Cuts at: 2048 3193 3432 3576 2048 Size: 1145 239 144 5187 Fragments arranged by size: 5187 1145 239 144 PstI C_TGCA'G Cuts at: 830 2177 2411 2880 3376 3414 3672 4067 830 Size: 1347 234 469 496 38 258 395 3478 Fragments arranged by size: 3478 1347 496 469 395 258 234 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6715 PvuII CAG'CTG Cuts at: 2679 2718 2679 Size: 39 6676 SacI G_AGCT'C Cuts at: 2012 2556 3009 3552 2012 Size: 544 453 543 5175 Fragments arranged by size: 5175 544 543 453 SacII CC_GC'GG Cuts at: 2216 2216 Size: 6715 SalI G'TCGA_C Cuts at: 1936 4057 1936 Size: 2121 4594 SapI GCTCTTCn'nnn_ Cuts at: 2561 2990 3242 3485 2561 Size: 429 252 243 5791 Fragments arranged by size: 5791 429 252 243 SbfI CC_TGCA'GG Cuts at: 4067 4067 Size: 6715 ScaI AGT'ACT Cuts at: 4963 6675 4963 Size: 1712 5003 SfcI C'TryA_G Cuts at: 826 1045 2173 2407 2455 2686 2876 3361 3372 Size: 219 1128 234 48 231 190 485 11 Cuts at: 3372 3410 3668 4063 5198 5879 6070 826 Size: 38 258 395 1135 681 191 1471 Fragments arranged by size: 1471 1135 1128 681 485 395 258 234 231 219 191 190 48 38 11 SfiI GGCCn_nnn'nGGCC Cuts at: 3192 3192 Size: 6715 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6715 SmaI CCC'GGG Cuts at: 1872 3962 4042 1872 Size: 2090 80 4545 Fragments arranged by size: 4545 2090 80 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 3008 4819 5720 5982 820 Size: 197 926 813 252 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 813 262 252 197 SnaBI TAC'GTA Cuts at: 493 4506 493 Size: 4013 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6715 SphI G_CATG'C Cuts at: 1132 2236 4073 1132 Size: 1104 1837 3774 Fragments arranged by size: 3774 1837 1104 SspI AAT'ATT Cuts at: 5 52 4639 5 Size: 47 4587 2081 Fragments arranged by size: 4587 2081 47 StuI AGG'CCT Cuts at: 1517 2692 3558 1517 Size: 1175 866 4674 Fragments arranged by size: 4674 1175 866 StyI C'CwwG_G Cuts at: 513 1065 3133 3222 3768 4154 513 Size: 552 2068 89 546 386 3074 Fragments arranged by size: 3074 2068 552 546 386 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3215 3559 4742 4759 4912 5097 5542 Size: 1255 1635 344 1183 17 153 185 445 Cuts at: 5542 325 Size: 1498 Fragments arranged by size: 1635 1498 1255 1183 445 344 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3603 4961 6673 Size: 274 80 33 51 1443 1626 1358 1712 Cuts at: 6673 96 Size: 138 Fragments arranged by size: 1712 1626 1443 1358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2282 2314 6675 6707 2282 Size: 32 4361 32 2290 Fragments arranged by size: 4361 2290 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2498 1316 Size: 1182 5533 XbaI T'CTAG_A Cuts at: 4051 4051 Size: 6715 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2614 3722 2614 Size: 1108 5607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6715 XmaI C'CCGG_G Cuts at: 1870 3960 4040 1870 Size: 2090 80 4545 Fragments arranged by size: 4545 2090 80 XmnI GAAnn'nnTTC Cuts at: 4844 4844 Size: 6715 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2074 3019 3675 4523 Size: 53 83 186 936 540 945 656 848 Cuts at: 4523 276 Size: 2468 Fragments arranged by size: 2468 945 936 848 656 540 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI