################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BstBI BstYI Bsu36I CelII ClaI CpoI DpnII DraI DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MboI MfeI MflI MluI MroI MseI MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 170 GTATAC GT^[AC][GT]AC AlwNI 454 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 464 GGGCCC GGGCC^C BalI 425 TGGCCA TGG^CCA BbvI 757 GCTGC GCAGC(8/12) BglI 119 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 552 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 695 TCATGA T^CATGA BspMI 583 ACCTGC ACCTGC(4/8) BsrBI 573 GAGCGG CCGCTC(-3/-3) BsrFI 31 GCCGGC [GA]^CCGG[CT] BssSI 469 CTCGTG CACGAG(-5/-1) Bst1107I 170 GTATAC GTA^TAC BstAPI 596 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 474 GGTCACC G^GT[ACGT]ACC BstPI 474 GGTCACC G^GT[ACGT]ACC BstXI 95 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 170 GTATAC GTA^TAC Cfr10I 31 GCCGGC [GA]^CCGG[CT] DraIII 597 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 11 CGGCCG C^GGCCG EclXI 11 CGGCCG C^GGCCG Eco52I 11 CGGCCG C^GGCCG EcoO65I 474 GGTCACC G^GT[ACGT]ACC KspI 219 CCGCGG CCGC^GG MaeIII 475 GTCAC ^GT[ACGT]AC MamI 552 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 425 TGGCCA TGG^CCA MscI 425 TGGCCA TGG^CCA MslI 267 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 219 CCGCGG C[AC]G^C[GT]G NaeI 31 GCCGGC GCC^GGC NgoMIV 31 GCCGGC G^CCGGC NspI 179 GCATGC [GA]CATG^[CT] PflMI 453 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 695 TCATGA T^CATGA SacII 219 CCGCGG CCGC^GG SexAI 383 ACCTGGT A^CC[AT]GGT SfaNI 257 GCATC GCATC(5/9) SfcI 528 CTGTAG C^T[GA][CT]AG SphI 179 GCATGC GCATG^C TseI 757 GCTGC G^C[AT]GC Tsp45I 475 GTCAC ^GT[GC]AC Van91I 453 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 34 GGCGTC G[GA]^CG[CT]C 795 GACGCC Alw44I 205 GTGCAC G^TGCAC 603 GTGCAC ApaLI 205 GTGCAC G^TGCAC 603 GTGCAC BanI 228 GGCACC G^G[CT][GA]CC 657 GGTGCC BanII 464 GGGCCC G[GA]GC[CT]^C 744 GAGCCC BsaHI 34 GGCGTC G[GA]^CG[CT]C 795 GACGCC BseRI 634 GAGGAG GAGGAG(10/8) 709 GAGGAG BsgI 594 CTGCAC GTGCAG(16/14) 758 CTGCAC BsiEI 11 CGGCCG CG[GA][CT]^CG 216 CGACCG BsrI 167 CCAGT ACTGG(1/-1) 210 CCAGT DdeI 60 CTCAG C^T[ACGT]AG 666 CTTAG EcoNI 524 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 705 CCTGGAGGAGG Hin1I 34 GGCGTC G[GA]^CG[CT]C 795 GACGCC MaeII 359 ACGT A^CGT 506 ACGT PpuMI 406 GGGACCT [GA]G^G[AT]CC[CT] 700 AGGACCC SmaI 402 CCCGGG CCC^GGG 739 CCCGGG XmaCI 402 CCCGGG C^CCGGG 739 CCCGGG XmaI 402 CCCGGG C^CCGGG 739 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AluI AspHI AvaI AvaII BbsI BcnI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TfiI TspRI