Restriction map for
pFN21AE2766
(Circular) (Six-base) MAPSORT of: pFN21AE2766.seq Check: 8435 from: 1 to: 5518
pFN21AE2766 5518 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2597 2597
Size: 5518
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2176 2861 1937
Size: 239 685 4594
Fragments arranged by size:
4594 685 239
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2225 1184
Size: 1041 4477
AlwNI CAG_nnn'CTG
Cuts at: 2464 3261 4833 2464
Size: 797 1572 3149
Fragments arranged by size:
3149 1572 797
ApaI G_GGCC'C
Cuts at: 2473 2473
Size: 5518
ApaLI G'TGCA_C
Cuts at: 2210 2608 3575 4731 2210
Size: 398 967 1156 2997
Fragments arranged by size:
2997 1156 967 398
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 2407 2744 2843 1870
Size: 73 246 218 337 99 4545
Fragments arranged by size:
4545 337 246 218 99 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2662 2839 4294 618
Size: 325 217 627 446 429 177 1455 1842
Fragments arranged by size:
1842 1455 627 446 429 325 217 177
BanII G_rGCy'C
Cuts at: 1391 2473 2753 2837 1391
Size: 1082 280 84 4072
Fragments arranged by size:
4072 1082 280 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2233 2546 2611 928
Size: 971 334 313 65 3835
Fragments arranged by size:
3835 971 334 313 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2130 4129
Size: 107 122 71 631 605 337 121 1999
Cuts at: 4129 136
Size: 1525
Fragments arranged by size:
1999 1525 631 605 337 122 121 107
71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2236 2473 2612 2667 2685 3579 4735
Size: 424 22 237 139 55 18 894 1156
Cuts at: 4735 1790
Size: 2573
Fragments arranged by size:
2573 1156 894 424 237 139 55 22
18
BmgBI CAC'GTC
Cuts at: 1318 2365 2512 1318
Size: 1047 147 4324
Fragments arranged by size:
4324 1047 147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2166 3249 4207
Size: 416 53 83 399 24 749 1083 958
Cuts at: 4207 442
Size: 1753
Fragments arranged by size:
1753 1083 958 749 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2608 2732 4178 1239
Size: 1073 296 124 1446 2579
Fragments arranged by size:
2579 1446 1073 296 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 2561 5512 2561
Size: 2951 2567
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2801 3325 3707
Size: 53 83 186 936 507 761 524 382
Cuts at: 3707 4342 275
Size: 635 1451
Fragments arranged by size:
1451 936 761 635 524 507 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2729 1186
Size: 1468 75 3975
Fragments arranged by size:
3975 1468 75
BseYI C'CCAG_C
Cuts at: 2229 2649 4721 5217 2229
Size: 420 2072 496 2530
Fragments arranged by size:
2530 2072 496 420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2584 2748 1163
Size: 573 848 164 3933
Fragments arranged by size:
3933 848 573 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2224 2968 3729 4757 1540
Size: 275 186 18 205 744 761 1028 2301
Fragments arranged by size:
2301 1028 761 744 275 205 186 18
BsiHKAI G_wGCw'C
Cuts at: 2214 2427 2612 3579 3664 4735 2214
Size: 213 185 967 85 1071 2997
Fragments arranged by size:
2997 1071 967 213 185 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2236 2427 2473 2612 2667 2685
Size: 399 424 22 191 46 139 55 18
Cuts at: 2685 2753 2837 3579 3664 4735 1391
Size: 68 84 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 191 139 85
84 68 55 46 22 18
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 2700 3407 5137 2700
Size: 707 1730 3081
Fragments arranged by size:
3081 1730 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 2873 844
Size: 1753 276 3489
Fragments arranged by size:
3489 1753 276
BsrBI CCG'CTC
Cuts at: 1394 1823 2580 3405 1394
Size: 429 757 825 3507
Fragments arranged by size:
3507 825 757 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 4162 4349 1700
Size: 110 226 2126 187 2869
Fragments arranged by size:
2869 2126 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 2474 3578 4590 2474
Size: 1104 1012 3402
Fragments arranged by size:
3402 1104 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2607 794
Size: 1813 3705
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2107
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2177 2177
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2224 2310 2502 513
Size: 552 939 96 124 86 192 3529
Fragments arranged by size:
3529 939 552 192 124 96 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 2596 3034 3828 3848 2117
Size: 479 438 794 20 3787
Fragments arranged by size:
3787 794 479 438 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DraIII CAC_nnn'GTG
Cuts at: 2607 2607
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2034 2430
Size: 54 1425 50 275 18 186 18 396
Cuts at: 2430 2965 3854 5483 8
Size: 535 889 1629 43
Fragments arranged by size:
1629 1425 889 535 396 275 186 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2965 1537
Size: 275 204 949 4090
Fragments arranged by size:
4090 949 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 1353
Size: 2095 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2649 4105 4479 4625 387
Size: 1064 138 1060 1456 374 146 1280
Fragments arranged by size:
1456 1280 1064 1060 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2608 2732 3581 4178 4965 5233
Size: 393 680 296 124 849 597 787 268
Cuts at: 5233 1239
Size: 1524
Fragments arranged by size:
1524 849 787 680 597 393 296 268
124
EcoNI CCTnn'n_nnAGG
Cuts at: 2533 2714 2533
Size: 181 5337
EcoO109I rG'GnC_Cy
Cuts at: 2324 2412 2469 2470 2706 2324
Size: 88 57 1 236 5136
Fragments arranged by size:
5136 236 88 57 1
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 5518
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 4665 1678
Size: 2987 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373
Size: 32 135 32 2587 32 42 32 2626
Fragments arranged by size:
2626 2587 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 4632 4816 1305
Size: 1080 2247 184 2007
Fragments arranged by size:
2247 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 3096
Fragments arranged by size:
3096 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3476 3835 3994 518
Size: 648 1110 1200 359 159 2042
Fragments arranged by size:
2042 1200 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2226 2934 3611 4759 5004 1606
Size: 620 708 677 1148 245 2120
Fragments arranged by size:
2120 1148 708 677 620 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 5518
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2188 2876 4421 1132
Size: 97 959 688 1545 2229
Fragments arranged by size:
2229 1545 959 688 97
PasI CC'CwG_GG
Cuts at: 1348 2464 1348
Size: 1116 4402
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 1108
Size: 1356 4162
PfoI T'CCnGG_A
Cuts at: 2148 2244 2733 2148
Size: 96 489 4933
Fragments arranged by size:
4933 489 96
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2706 2412
Size: 294 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2469 2469
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
SacII CC_GC'GG
Cuts at: 2227 2227
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2388 2388
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2533 2866 4001 4682 4873 826
Size: 219 1488 333 1135 681 191 1471
Fragments arranged by size:
1488 1471 1135 681 333 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2409 2746 2845 1872
Size: 537 337 99 4545
Fragments arranged by size:
4545 537 337 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2188 2876 1132
Size: 1056 688 3774
Fragments arranged by size:
3774 1056 688
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2957 513
Size: 552 939 953 3074
Fragments arranged by size:
3074 953 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 5478 5510 2226
Size: 32 3220 32 2234
Fragments arranged by size:
3220 2234 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2407 2744 2843 1870
Size: 537 337 99 4545
Fragments arranged by size:
4545 537 337 99
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI
SrfI SwaI XcmI