################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaHI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 833 GGTACC G^GTACC AccI 760 GTCTAC GT^[AC][GT]AC AccII 500 CGCG CG^CG AflIII 182 ACGTGT A^C[GA][CT]GT AlwNI 824 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 636 GGGCCC GGGCC^C Asp718I 833 GGTACC G^GTACC BbrPI 181 CACGTG CAC^GTG BfaI 889 CTAG C^TAG BlpI 201 GCTCAGC GC^T[ACGT]AGC Bpu1102I 201 GCTCAGC GC^T[ACGT]AGC BsaI 568 GAGACC GGTCTC(1/5) BsaWI 470 TCCGGT [AT]^CCGG[AT] BssSI 767 CTCGTG CACGAG(-5/-1) BstEII 480 GGTGACC G^GT[ACGT]ACC BstPI 480 GGTGACC G^GT[ACGT]ACC BstUI 500 CGCG CG^CG CelII 201 GCTCAGC GC^T[ACGT]AGC CfoI 140 GCGC GCG^C EarI 877 CTCTTC CTCTTC(1/4) EcoO65I 480 GGTGACC G^GT[ACGT]ACC FseI 79 GGCCGGCC GGCCGG^CC HhaI 140 GCGC GCG^C HinP1I 140 GCGC G^CGC KpnI 833 GGTACC GGTAC^C Ksp632I 877 CTCTTC CTCTTC(1/4) MaeI 889 CTAG C^TAG MslI 143 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 500 CGCG CG^CG NcoI 148 CCATGG C^CATGG NotI 737 GCGGCCGC GC^GGCCGC NspI 620 ACATGC [GA]CATG^[CT] PflMI 70 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 181 CACGTG CAC^GTG PmlI 181 CACGTG CAC^GTG PpuMI 244 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 224 CAGCTG CAG^CTG SacI 388 GAGCTC GAGCT^C SexAI 266 ACCAGGT A^CC[AT]GGT SfiI 3 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 443 AGGCCT AGG^CCT Van91I 70 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 178 GTGCAC G^TGCAC 271 GTGCAC AocI 54 CCTCAGG CC^T[ACGT]AGG 347 CCTCAGG ApaLI 178 GTGCAC G^TGCAC 271 GTGCAC AvaII 245 GGTCC G^G[AT]CC 362 GGACC BalI 145 TGGCCA TGG^CCA 908 TGGCCA BanI 694 GGTGCC G^G[CT][GA]CC 833 GGTACC BglI 4 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 593 GCCCCGGGGGC BsaAI 181 CACGTG [CT]AC^GT[GA] 781 TACGTG BspMI 571 ACCTGC ACCTGC(4/8) 87 GCAGGT Bsu36I 54 CCTCAGG CC^T[ACGT]AGG 347 CCTCAGG DsaI 148 CCATGG C^C[GA][CT]GG 554 CCGTGG Eco81I 54 CCTCAGG CC^T[ACGT]AGG 347 CCTCAGG EcoNI 435 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 534 CCTGCGGGAGG EcoT14I 6 CCTTGG C^C[AT][AT]GG 148 CCATGG MaeII 182 ACGT A^CGT 782 ACGT MboII 863 TCTTC GAAGA(8/7) 878 TCTTC MluNI 145 TGGCCA TGG^CCA 908 TGGCCA MscI 145 TGGCCA TGG^CCA 908 TGGCCA MspA1I 224 CAGCTG C[AC]G^C[GT]G 366 CAGCGG NaeI 80 GCCGGC GCC^GGC 136 GCCGGC NgoMIV 80 GCCGGC G^CCGGC 136 GCCGGC StyI 6 CCTTGG C^C[AT][AT]GG 148 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI BamHI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TseI Tsp45I TspRI XhoII XmaCI XmaI