(Circular) (Six-base) MAPSORT of: pFN21AE6356.seq Check: 8467 from: 1 to: 5668 pFN21AE6356 5668 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2766 3011 1937 Size: 829 245 4594 Fragments arranged by size: 4594 829 245 Acc65I G'GTAC_C Cuts at: 2838 2989 2838 Size: 151 5517 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 3731 5115 5383 1632 Size: 968 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1131 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 2187 4567 5544 1203 Size: 984 2380 977 1327 Fragments arranged by size: 2380 1327 984 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2952 1184 Size: 1768 3900 AlwNI CAG_nnn'CTG Cuts at: 2834 3411 4983 2834 Size: 577 1572 3519 Fragments arranged by size: 3519 1572 577 ApaI G_GGCC'C Cuts at: 2645 2645 Size: 5668 ApaLI G'TGCA_C Cuts at: 2183 2276 3725 4881 2183 Size: 93 1449 1156 2970 Fragments arranged by size: 2970 1449 1156 93 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2600 2637 2645 2993 1870 Size: 73 657 37 8 348 4545 Fragments arranged by size: 4545 657 348 73 37 8 BamHI G'GATC_C Cuts at: 2424 2472 2796 2998 3365 2424 Size: 48 324 202 367 4727 Fragments arranged by size: 4727 367 324 202 48 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2699 2838 2989 4444 618 Size: 325 217 627 912 139 151 1455 1842 Fragments arranged by size: 1842 1455 912 627 325 217 151 139 BanII G_rGCy'C Cuts at: 1391 2397 2645 2828 2987 1391 Size: 1006 248 183 159 4072 Fragments arranged by size: 4072 1006 248 183 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2604 4279 Size: 107 122 71 631 605 343 589 1675 Cuts at: 4279 136 Size: 1525 Fragments arranged by size: 1675 1525 631 605 589 343 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 2207 3085 2207 Size: 878 4790 Bme1580I G_kGCm'C Cuts at: 1790 2049 2187 2280 2638 2645 3729 4885 1790 Size: 259 138 93 358 7 1084 1156 2573 Fragments arranged by size: 2573 1156 1084 358 259 138 93 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2209 3399 4357 Size: 416 53 83 399 24 792 1190 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1190 958 792 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2329 2401 4328 1239 Size: 1073 17 72 1927 2579 Fragments arranged by size: 2579 1927 1073 72 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2567 4331 882 Size: 617 1068 1764 2219 Fragments arranged by size: 2219 1764 1068 617 BsaAI yAC'GTr Cuts at: 493 1204 2188 2788 3459 493 Size: 711 984 600 671 2702 Fragments arranged by size: 2702 984 711 671 600 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2475 4101 4499 4773 4920 5331 2475 Size: 1626 398 274 147 411 2812 Fragments arranged by size: 2812 1626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 2290 2320 1178 Size: 30 165 30 825 30 32 30 4526 Fragments arranged by size: 4526 825 165 32 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5668 BseYI C'CCAG_C Cuts at: 2481 2493 2727 4871 5367 2481 Size: 12 234 2144 496 2782 Fragments arranged by size: 2782 2144 496 234 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 2729 2912 1163 Size: 573 588 405 183 3919 Fragments arranged by size: 3919 588 573 405 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2090 2113 2142 2746 3118 3879 Size: 275 186 89 23 29 604 372 761 Cuts at: 3879 4907 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 604 372 275 186 89 29 23 BsiHKAI G_wGCw'C Cuts at: 2187 2226 2280 2397 3729 3814 4885 2187 Size: 39 54 117 1332 85 1071 2970 Fragments arranged by size: 2970 1332 1071 117 85 54 39 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2187 2226 2280 2397 2638 2645 Size: 399 259 138 39 54 117 241 7 Cuts at: 2645 2828 2987 3729 3814 4885 1391 Size: 183 159 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 259 241 183 159 138 117 85 54 39 7 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 2585 3023 844 Size: 1239 502 438 3489 Fragments arranged by size: 3489 1239 502 438 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2141 2489 4312 4499 1700 Size: 110 275 56 348 1823 187 2869 Fragments arranged by size: 2869 1823 348 275 187 110 56 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 2772 3728 4740 2772 Size: 956 1012 3700 Fragments arranged by size: 3700 1012 956 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 2485 1184 Size: 1301 4367 BstYI r'GATC_y Cuts at: 1972 2424 2472 2796 2998 3365 3750 3767 5219 Size: 452 48 324 202 367 385 17 1452 Cuts at: 5219 5305 5317 5663 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 452 385 367 346 324 202 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 2060 2353 2060 Size: 293 5375 BtgI C'CryG_G Cuts at: 513 1065 2004 2153 2559 513 Size: 552 939 149 406 3622 Fragments arranged by size: 3622 939 552 406 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2365 487 Size: 683 56 1139 3790 Fragments arranged by size: 3790 1139 683 56 BtsI GCAGTG_nn' Cuts at: 2201 2236 3184 3978 3998 2201 Size: 35 948 794 20 3871 Fragments arranged by size: 3871 948 794 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2087 2110 Size: 54 1425 50 275 18 253 4 23 Cuts at: 2110 2139 2150 2743 2913 3115 4004 5633 8 Size: 29 11 593 170 202 889 1629 43 Fragments arranged by size: 1629 1425 889 593 275 253 202 170 54 50 43 29 23 18 11 4 EagI C'GGCC_G Cuts at: 1537 1812 2087 2110 2139 2743 3115 1537 Size: 275 275 23 29 604 372 4090 Fragments arranged by size: 4090 604 372 275 275 29 23 EarI CTCTTCn'nnn_ Cuts at: 1353 2888 3598 1353 Size: 1535 710 3423 Fragments arranged by size: 3423 1535 710 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 2789 4255 4629 4775 387 Size: 1064 138 570 630 1466 374 146 1280 Fragments arranged by size: 1466 1280 1064 630 570 374 146 138 EcoICRI GAG'CTC Cuts at: 2395 2395 Size: 5668 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2329 2401 2600 3731 4328 5115 Size: 393 680 17 72 199 1131 597 787 Cuts at: 5115 5383 1239 Size: 268 1524 Fragments arranged by size: 1524 1131 787 680 597 393 268 199 72 17 EcoNI CCTnn'n_nnAGG Cuts at: 2444 2543 2444 Size: 99 5569 EcoO109I rG'GnC_Cy Cuts at: 2016 2250 2641 2642 2675 2016 Size: 234 391 1 33 5009 Fragments arranged by size: 5009 391 234 33 1 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FseI GG_CCGG'CC Cuts at: 2089 2089 Size: 5668 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 4815 1678 Size: 3137 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373 Size: 32 135 32 2737 32 42 32 2626 Fragments arranged by size: 2737 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2842 2993 2842 Size: 151 5517 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 4782 4966 1305 Size: 984 2493 184 2007 Fragments arranged by size: 2493 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2152 2915 10 Size: 54 1425 343 320 763 2763 Fragments arranged by size: 2763 1425 763 343 320 54 MslI CAynn'nnrTG Cuts at: 518 1166 2152 3626 3985 4144 518 Size: 648 986 1474 359 159 2042 Fragments arranged by size: 2042 1474 986 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 2373 3084 3761 4909 5154 1606 Size: 625 142 711 677 1148 245 2120 Fragments arranged by size: 2120 1148 711 677 625 245 142 NaeI GCC'GGC Cuts at: 2087 2143 2087 Size: 56 5612 NcoI C'CATG_G Cuts at: 513 1065 2004 2153 513 Size: 552 939 149 4028 Fragments arranged by size: 4028 939 552 149 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NgoMIV G'CCGG_C Cuts at: 2085 2141 2085 Size: 56 5612 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 2743 3115 2743 Size: 372 5296 NspI r_CATG'y Cuts at: 1132 1229 2629 3026 4571 1132 Size: 97 1400 397 1545 2229 Fragments arranged by size: 2229 1545 1400 397 97 PasI CC'CwG_GG Cuts at: 1348 2468 1348 Size: 1120 4548 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2081 1108 Size: 973 4695 PfoI T'CCnGG_A Cuts at: 2442 2442 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PmlI CAC'GTG Cuts at: 2188 2188 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2250 2250 Size: 5668 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PspOMI G'GGCC_C Cuts at: 2641 2641 Size: 5668 PstI C_TGCA'G Cuts at: 830 3020 830 Size: 2190 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2231 2231 Size: 5668 SacI G_AGCT'C Cuts at: 2397 2397 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SanDI GG'GwC_CC Cuts at: 2250 2250 Size: 5668 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SexAI A'CCwGG_T Cuts at: 2271 2271 Size: 5668 SfcI C'TryA_G Cuts at: 826 1045 3016 4151 4832 5023 826 Size: 219 1971 1135 681 191 1471 Fragments arranged by size: 1971 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2015 2015 Size: 5668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2602 2639 2647 2995 1872 Size: 730 37 8 348 4545 Fragments arranged by size: 4545 730 348 37 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2450 1517 Size: 933 4735 StyI C'CwwG_G Cuts at: 513 1065 2004 2011 2153 3107 513 Size: 552 939 7 142 954 3074 Fragments arranged by size: 3074 954 939 552 142 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2209 2473 2695 2946 3695 3712 3865 Size: 1255 629 264 222 251 749 17 153 Cuts at: 3865 4050 4495 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 749 629 445 264 251 222 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 2290 2322 5628 5660 2228 Size: 32 30 32 3306 32 2236 Fragments arranged by size: 3306 2236 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2600 2637 2645 2993 1870 Size: 730 37 8 348 4545 Fragments arranged by size: 4545 730 348 37 8 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI XcmI