(Linear) (Six-base) MAPSORT of: KIEE6356.seq Check: 1066 from: 1 to: 963 KIEE6356.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AccI GT'mk_AC Cuts at: 0 761 963 Size: 761 202 Acc65I G'GTAC_C Cuts at: 0 833 963 Size: 833 130 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 595 963 Size: 595 368 AflIII A'CryG_T Cuts at: 0 182 963 Size: 182 781 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 947 963 Size: 947 16 AlwNI CAG_nnn'CTG Cuts at: 0 829 963 Size: 829 134 ApaI G_GGCC'C Cuts at: 0 640 963 Size: 640 323 ApaLI G'TGCA_C Cuts at: 0 178 271 963 Size: 178 93 692 Fragments arranged by size: 692 178 93 AvaI C'yCGr_G Cuts at: 0 595 632 640 963 Size: 595 37 8 323 Fragments arranged by size: 595 323 37 8 BamHI G'GATC_C Cuts at: 0 419 467 791 963 Size: 419 48 324 172 Fragments arranged by size: 419 324 172 48 BanI G'GyrC_C Cuts at: 0 694 833 963 Size: 694 139 130 Fragments arranged by size: 694 139 130 BanII G_rGCy'C Cuts at: 0 392 640 823 963 Size: 392 248 183 140 Fragments arranged by size: 392 248 183 140 BglI GCCn_nnn'nGGC Cuts at: 0 10 599 963 Size: 10 589 364 Fragments arranged by size: 589 364 10 BlpI GC'TnA_GC Cuts at: 0 202 963 Size: 202 761 Bme1580I G_kGCm'C Cuts at: 0 44 182 275 633 640 963 Size: 44 138 93 358 7 323 Fragments arranged by size: 358 323 138 93 44 7 BmrI ACTGGGnnnn_n' Cuts at: 0 204 963 Size: 204 759 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 324 396 963 Size: 307 17 72 567 Fragments arranged by size: 567 307 72 17 BsaI GGTCTCn'nnnn_ Cuts at: 0 562 963 Size: 562 401 BsaAI yAC'GTr Cuts at: 0 183 783 963 Size: 183 600 180 Fragments arranged by size: 600 183 180 BsaWI w'CCGG_w Cuts at: 0 470 963 Size: 470 493 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 223 253 285 315 963 Size: 223 30 32 30 648 Fragments arranged by size: 648 223 32 30 30 BseYI C'CCAG_C Cuts at: 0 476 488 722 963 Size: 476 12 234 241 Fragments arranged by size: 476 241 234 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 724 907 963 Size: 319 405 183 56 Fragments arranged by size: 405 319 183 56 BsiEI CG_ry'CG Cuts at: 0 85 108 137 741 963 Size: 85 23 29 604 222 Fragments arranged by size: 604 222 85 29 23 BsiHKAI G_wGCw'C Cuts at: 0 182 221 275 392 963 Size: 182 39 54 117 571 Fragments arranged by size: 571 182 117 54 39 Bsp1286I G_dGCh'C Cuts at: 0 44 182 221 275 392 633 640 823 Size: 44 138 39 54 117 241 7 183 Cuts at: 823 963 Size: 140 Fragments arranged by size: 241 183 140 138 117 54 44 39 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 78 580 963 Size: 78 502 383 Fragments arranged by size: 502 383 78 BsrFI r'CCGG_y Cuts at: 0 80 136 484 963 Size: 80 56 348 479 Fragments arranged by size: 479 348 80 56 BssSI C'ACGA_G Cuts at: 0 767 963 Size: 767 196 BstEII G'GTnAC_C Cuts at: 0 480 963 Size: 480 483 BstYI r'GATC_y Cuts at: 0 419 467 791 963 Size: 419 48 324 172 Fragments arranged by size: 419 324 172 48 Bsu36I CC'TnA_GG Cuts at: 0 55 348 963 Size: 55 293 615 Fragments arranged by size: 615 293 55 BtgI C'CryG_G Cuts at: 0 148 554 963 Size: 148 406 409 Fragments arranged by size: 409 406 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 360 963 Size: 360 603 BtsI GCAGTG_nn' Cuts at: 0 196 231 963 Size: 196 35 732 Fragments arranged by size: 732 196 35 EaeI y'GGCC_r Cuts at: 0 78 82 105 134 145 738 908 963 Size: 78 4 23 29 11 593 170 55 Fragments arranged by size: 593 170 78 55 29 23 11 4 EagI C'GGCC_G Cuts at: 0 82 105 134 738 963 Size: 82 23 29 604 225 Fragments arranged by size: 604 225 82 29 23 EarI CTCTTCn'nnn_ Cuts at: 0 883 963 Size: 883 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 154 784 963 Size: 154 630 179 Fragments arranged by size: 630 179 154 EcoICRI GAG'CTC Cuts at: 0 390 963 Size: 390 573 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 324 396 595 963 Size: 307 17 72 199 368 Fragments arranged by size: 368 307 199 72 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 439 538 963 Size: 439 99 425 Fragments arranged by size: 439 425 99 EcoO109I rG'GnC_Cy Cuts at: 0 11 245 636 637 670 963 Size: 11 234 391 1 33 293 Fragments arranged by size: 391 293 234 33 11 1 FseI GG_CCGG'CC Cuts at: 0 84 963 Size: 84 879 KpnI G_GTAC'C Cuts at: 0 837 963 Size: 837 126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 284 963 Size: 284 679 MscI TGG'CCA Cuts at: 0 147 910 963 Size: 147 763 53 Fragments arranged by size: 763 147 53 MslI CAynn'nnrTG Cuts at: 0 147 963 Size: 147 816 MspA1I CmG'CkG Cuts at: 0 226 368 963 Size: 226 142 595 Fragments arranged by size: 595 226 142 NaeI GCC'GGC Cuts at: 0 82 138 963 Size: 82 56 825 Fragments arranged by size: 825 82 56 NcoI C'CATG_G Cuts at: 0 148 963 Size: 148 815 NgoMIV G'CCGG_C Cuts at: 0 80 136 963 Size: 80 56 827 Fragments arranged by size: 827 80 56 NotI GC'GGCC_GC Cuts at: 0 738 963 Size: 738 225 NspI r_CATG'y Cuts at: 0 624 963 Size: 624 339 PasI CC'CwG_GG Cuts at: 0 463 963 Size: 463 500 PflMI CCAn_nnn'nTGG Cuts at: 0 76 963 Size: 76 887 PfoI T'CCnGG_A Cuts at: 0 437 963 Size: 437 526 PmlI CAC'GTG Cuts at: 0 183 963 Size: 183 780 PpuMI rG'GwC_Cy Cuts at: 0 245 963 Size: 245 718 PspOMI G'GGCC_C Cuts at: 0 636 963 Size: 636 327 PvuII CAG'CTG Cuts at: 0 226 963 Size: 226 737 SacI G_AGCT'C Cuts at: 0 392 963 Size: 392 571 SanDI GG'GwC_CC Cuts at: 0 245 963 Size: 245 718 SexAI A'CCwGG_T Cuts at: 0 266 963 Size: 266 697 SfiI GGCCn_nnn'nGGCC Cuts at: 0 10 963 Size: 10 953 SmaI CCC'GGG Cuts at: 0 597 634 642 963 Size: 597 37 8 321 Fragments arranged by size: 597 321 37 8 StuI AGG'CCT Cuts at: 0 445 963 Size: 445 518 StyI C'CwwG_G Cuts at: 0 6 148 963 Size: 6 142 815 Fragments arranged by size: 815 142 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 204 468 690 941 963 Size: 204 264 222 251 22 Fragments arranged by size: 264 251 222 204 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 223 255 285 317 963 Size: 223 32 30 32 646 Fragments arranged by size: 646 223 32 32 30 XmaI C'CCGG_G Cuts at: 0 595 632 640 963 Size: 595 37 8 323 Fragments arranged by size: 595 323 37 8 Enzymes that do cut: AccI Acc65I AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BglI BlpI Bme1580I BmrI BpmI BsaI BsaAI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PvuII SacI SanDI SexAI SfiI SmaI StuI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI