################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstF5I BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AlwI 300 GATCC GGATC(4/5) Aor51HI 602 AGCGCT AGC^GCT ApaI 213 GGGCCC GGGCC^C AscI 867 GGCGCGCC GG^CGCGCC Asp700I 462 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 284 TGGCCA TGG^CCA BbrPI 835 CACGTG CAC^GTG BcgI 1213 CGATGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1120 CTAG C^TAG BsiEI 274 CGGCCG CG[GA][CT]^CG BsmBI 525 CGTCTC CGTCTC(1/5) BsrBI 271 GAGCGG CCGCTC(-3/-3) BsrFI 414 GCCGGT [GA]^CCGG[CT] BsrI 1162 ACTGG ACTGG(1/-1) BstEII 996 GGTCACC G^GT[ACGT]ACC BstPI 996 GGTCACC G^GT[ACGT]ACC BstXI 80 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 299 AGATCC [GA]^GATC[CT] Cfr10I 414 GCCGGT [GA]^CCGG[CT] DraII 212 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 928 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 274 CGGCCG C^GGCCG EclXI 274 CGGCCG C^GGCCG Eco47III 602 AGCGCT AGC^GCT Eco52I 274 CGGCCG C^GGCCG EcoO109I 212 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 996 GGTCACC G^GT[ACGT]ACC HincII 937 GTCAAC GT[CT]^[GA]AC HindII 937 GTCAAC GT[CT]^[GA]AC MaeI 1120 CTAG C^TAG MflI 299 AGATCC [GA]^GATC[CT] MluNI 284 TGGCCA TGG^CCA MscI 284 TGGCCA TGG^CCA NotI 273 GCGGCCGC GC^GGCCGC NspI 423 GCATGC [GA]CATG^[CT] PaeR7I 628 CTCGAG C^TCGAG PmaCI 835 CACGTG CAC^GTG PmlI 835 CACGTG CAC^GTG SgrAI 413 CGCCGGTG C[GA]^CCGG[CT]G SphI 423 GCATGC GCATG^C XcmI 923 CCAACCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 628 CTCGAG C^TCGAG XhoII 299 AGATCC [GA]^GATC[CT] XmnI 462 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 523 GGCGTC G[GA]^CG[CT]C 772 GACGCC Alw44I 219 GTGCAC G^TGCAC 946 GTGCAC ApaLI 219 GTGCAC G^TGCAC 946 GTGCAC AvaII 235 GGACC G^G[AT]CC 710 GGTCC AviII 1034 TGCGCA TGC^GCA 1043 TGCGCA BbvCI 242 CCTCAGC CCTCAGC(-5/-2) 1167 CCTCAGC BsaAI 835 CACGTG [CT]AC^GT[GA] 1096 TACGTG BsaHI 523 GGCGTC G[GA]^CG[CT]C 772 GACGCC BseRI 1123 GAGGAG GAGGAG(10/8) 1348 GAGGAG BsgI 1111 CTGCAC GTGCAG(16/14) 1330 GTGCAG BsmAI 526 GTCTC GTCTC(1/5) 1281 GTCTC BsmFI 192 GTCCC GGGAC(10/14) 1191 GTCCC DpnII 300 GATC ^GATC 918 GATC DsaI 287 CCACGG C^C[GA][CT]GG 927 CCACGG EarI 313 CTCTTC CTCTTC(1/4) 1390 CTCTTC FauI 1296 CCCGC CCCGC(4/6) 1418 CCCGC FspI 1034 TGCGCA TGC^GCA 1043 TGCGCA Hin1I 523 GGCGTC G[GA]^CG[CT]C 772 GACGCC Ksp632I 313 CTCTTC CTCTTC(1/4) 1390 CTCTTC MboI 300 GATC ^GATC 918 GATC NdeII 300 GATC ^GATC 918 GATC PflMI 392 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 927 CCACGGCGTGG PvuII 793 CAGCTG CAG^CTG 1333 CAGCTG SapI 312 GCTCTTC GCTCTTC(1/4) 1389 GCTCTTC Sau3AI 300 GATC ^GATC 918 GATC SbfI 477 CCTGCAGG CCTGCA^GG 822 CCTGCAGG SmlI 598 CTCAAG C^T[CT][GA]AG 628 CTCGAG Sse8387I 477 CCTGCAGG CCTGCA^GG 822 CCTGCAGG TspRI 188 CACTG CA[GC]TG[ACGT][ACGT]^ 1161 CACTG Van91I 392 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 927 CCACGGCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI BanI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BspMI BssHII BssKI BssSI BstAPI BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI EaeI EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI RsaI SacI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TseI Tsp45I TthHB8I XmaCI XmaI