(Circular) (Six-base) MAPSORT of: pFN21AE4109.seq Check: 9608 from: 1 to: 6142 pFN21AE4109 6142 bp With 182 enzymes: SgfI * March 28, 2012 13:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2225 3426 2225 Size: 1201 4941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3269 4205 5589 5857 1632 Size: 1637 936 1384 268 1917 Fragments arranged by size: 1917 1637 1384 936 268 AfeI AGC'GCT Cuts at: 2609 2609 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 2420 1166 Size: 1254 4888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2508 1184 Size: 1324 4818 AlwNI CAG_nnn'CTG Cuts at: 2803 3349 3418 3885 5457 2803 Size: 546 69 467 1572 3488 Fragments arranged by size: 3488 1572 546 467 69 ApaI G_GGCC'C Cuts at: 2222 2222 Size: 6142 ApaLI G'TGCA_C Cuts at: 2224 2951 4199 5355 2224 Size: 727 1248 1156 3011 Fragments arranged by size: 3011 1248 1156 727 ApoI r'AATT_y Cuts at: 1493 3451 3677 3829 5938 1493 Size: 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1697 226 152 AscI GG'CGCG_CC Cuts at: 2873 2873 Size: 6142 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2199 2633 2986 3377 3389 3467 Size: 73 71 185 434 353 391 12 78 Cuts at: 3467 1870 Size: 4545 Fragments arranged by size: 4545 434 391 353 185 78 73 71 12 BamHI G'GATC_C Cuts at: 3472 3839 3472 Size: 367 5775 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2257 2265 3069 3463 4918 Size: 325 217 627 470 8 804 394 1455 Cuts at: 4918 618 Size: 1842 Fragments arranged by size: 1842 1455 804 627 470 394 325 217 8 BanII G_rGCy'C Cuts at: 1391 2015 2222 2319 2442 3024 3135 3396 3461 Size: 624 207 97 123 582 111 261 65 Cuts at: 3461 1391 Size: 4072 Fragments arranged by size: 4072 624 582 261 207 123 111 97 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5171 BbvCI CC'TCA_GC Cuts at: 2248 3173 2248 Size: 925 5217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3207 3241 4333 4367 1903 Size: 34 1270 34 1092 34 3678 Fragments arranged by size: 3678 1270 1092 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 2786 2988 Size: 107 122 71 631 605 854 260 202 Cuts at: 2988 4753 136 Size: 1765 1525 Fragments arranged by size: 1765 1525 854 631 605 260 202 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 2744 2822 2978 3559 2744 Size: 78 156 581 5327 Fragments arranged by size: 5327 581 156 78 Bme1580I G_kGCm'C Cuts at: 1790 2120 2222 2228 2268 2955 3074 4203 5359 Size: 330 102 6 40 687 119 1129 1156 Cuts at: 5359 1790 Size: 2573 Fragments arranged by size: 2573 1156 1129 687 330 119 102 40 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2693 3116 3371 3380 4802 1239 Size: 1454 423 255 9 1422 2579 Fragments arranged by size: 2579 1454 1422 423 255 9 Bpu10I CC'TnA_GC Cuts at: 2248 2723 3173 2248 Size: 475 450 5217 Fragments arranged by size: 5217 475 450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2588 4267 5132 5430 2588 Size: 1679 865 298 3300 Fragments arranged by size: 3300 1679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2842 3103 3933 493 Size: 711 1638 261 830 2702 Fragments arranged by size: 2702 1638 830 711 261 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2529 2778 3949 4331 Size: 53 83 186 936 996 249 1171 382 Cuts at: 4331 4966 275 Size: 635 1451 Fragments arranged by size: 1451 1171 996 936 635 382 249 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 1178 Size: 30 165 30 748 30 5139 Fragments arranged by size: 5139 748 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3143 3368 1186 Size: 1957 225 3960 Fragments arranged by size: 3960 1957 225 BseYI C'CCAG_C Cuts at: 2177 2213 2269 2916 5345 5841 2177 Size: 36 56 647 2429 496 2478 Fragments arranged by size: 2478 2429 647 496 56 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3101 3356 1163 Size: 573 1365 255 3949 Fragments arranged by size: 3949 1365 573 255 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2282 3592 4353 5381 1540 Size: 275 186 281 1310 761 1028 2301 Fragments arranged by size: 2301 1310 1028 761 281 275 186 BsiHKAI G_wGCw'C Cuts at: 2228 2442 2955 3024 3135 3141 3396 4203 4288 Size: 214 513 69 111 6 255 807 85 Cuts at: 4288 5359 2228 Size: 1071 3011 Fragments arranged by size: 3011 1071 807 513 255 214 111 85 69 6 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 BsmBI CGTCTCn'nnnn_ Cuts at: 2536 2536 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2120 2222 2228 2268 2319 2442 Size: 399 225 105 102 6 40 51 123 Cuts at: 2442 2955 3024 3074 3135 3141 3396 3461 4203 Size: 513 69 50 61 6 255 65 742 Cuts at: 4203 4288 5359 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 399 255 225 123 105 102 85 69 65 61 51 50 40 6 6 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 2490 3045 3123 3222 3426 3497 844 Size: 1381 265 555 78 99 204 71 3489 Fragments arranged by size: 3489 1381 555 265 204 99 78 71 BsrBI CCG'CTC Cuts at: 1394 1823 2278 4029 1394 Size: 429 455 1751 3507 Fragments arranged by size: 3507 1751 455 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2419 4786 4973 1700 Size: 110 609 2367 187 2869 Fragments arranged by size: 2869 2367 609 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 2727 2729 2873 1925 Size: 802 2 144 5194 Fragments arranged by size: 5194 802 144 2 BssSI C'ACGA_G Cuts at: 3023 3064 3134 4202 5214 3023 Size: 41 70 1068 1012 3951 Fragments arranged by size: 3951 1068 1012 70 41 BstAPI GCAn_nnn'nTGC Cuts at: 794 2803 2899 2959 3418 794 Size: 2009 96 60 459 3518 Fragments arranged by size: 3518 2009 459 96 60 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 3001 1184 Size: 1817 4325 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2092 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2304 3472 3839 4224 4241 5693 5779 5791 Size: 332 1168 367 385 17 1452 86 12 Cuts at: 5791 6137 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1168 385 367 346 332 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2292 2932 513 Size: 552 1227 640 3723 Fragments arranged by size: 3723 1227 640 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2767 3232 487 Size: 683 56 1541 465 3397 Fragments arranged by size: 3397 1541 683 465 56 BtsI GCAGTG_nn' Cuts at: 3658 4452 4472 3658 Size: 794 20 5328 Fragments arranged by size: 5328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DraIII CAC_nnn'GTG Cuts at: 2938 2938 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2279 2289 Size: 54 1425 50 275 18 270 179 10 Cuts at: 2289 2769 2787 3027 3589 4478 6107 8 Size: 480 18 240 562 889 1629 43 Fragments arranged by size: 1629 1425 889 562 480 275 270 240 179 54 50 43 18 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2279 3589 1537 Size: 275 467 1310 4090 Fragments arranged by size: 4090 1310 467 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2324 3401 4072 1353 Size: 971 1077 671 3423 Fragments arranged by size: 3423 1077 971 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2831 4729 5103 5249 387 Size: 1064 138 1242 1898 374 146 1280 Fragments arranged by size: 1898 1280 1242 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2440 3022 3133 3394 2440 Size: 582 111 261 5188 Fragments arranged by size: 5188 582 261 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2693 3116 3269 3371 3380 4205 4802 Size: 393 1061 423 153 102 9 825 597 Cuts at: 4802 5589 5857 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1061 825 787 597 423 393 268 153 102 9 EcoO109I rG'GnC_Cy Cuts at: 2218 2218 Size: 6142 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 3041 3050 4648 3041 Size: 9 1598 4535 Fragments arranged by size: 4535 1598 9 HaeII r_GCGC'y Cuts at: 1678 2611 2858 3095 5289 1678 Size: 933 247 237 2194 2531 Fragments arranged by size: 2531 2194 933 247 237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4783 4815 4857 4889 1373 Size: 32 135 32 3211 32 42 32 2626 Fragments arranged by size: 3211 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2944 3486 3732 669 Size: 988 281 1006 542 246 3079 Fragments arranged by size: 3079 1006 988 542 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2559 5256 5440 1305 Size: 1254 2697 184 2007 Fragments arranged by size: 2697 2007 1254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 10 Size: 54 1425 343 459 3861 Fragments arranged by size: 3861 1425 459 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 2765 3086 4100 4459 4618 518 Size: 648 1254 345 321 1014 359 159 2042 Fragments arranged by size: 2042 1254 1014 648 359 345 321 159 MspA1I CmG'CkG Cuts at: 1606 2273 2287 2800 3304 3340 3426 3558 4235 Size: 667 14 513 504 36 86 132 677 Cuts at: 4235 5383 5628 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 667 513 504 245 132 86 36 14 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5590 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 2279 3589 2279 Size: 1310 4832 NspI r_CATG'y Cuts at: 1132 1229 2432 3500 5045 1132 Size: 97 1203 1068 1545 2229 Fragments arranged by size: 2229 1545 1203 1068 97 PasI CC'CwG_GG Cuts at: 1348 3265 1348 Size: 1917 4225 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2403 2938 1108 Size: 1295 535 4312 Fragments arranged by size: 4312 1295 535 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PmlI CAC'GTG Cuts at: 2842 2842 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 2488 4211 4243 5724 5756 2456 Size: 32 1723 32 1481 32 2842 Fragments arranged by size: 2842 1723 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 2218 2218 Size: 6142 PspXI vC'TCGA_Gb Cuts at: 2633 2633 Size: 6142 PstI C_TGCA'G Cuts at: 830 2487 2832 3345 3494 830 Size: 1657 345 513 149 3478 Fragments arranged by size: 3478 1657 513 345 149 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2800 3340 2800 Size: 540 5602 SacI G_AGCT'C Cuts at: 2442 3024 3135 3396 2442 Size: 582 111 261 5188 Fragments arranged by size: 5188 582 261 111 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SapI GCTCTTCn'nnn_ Cuts at: 2324 3401 2324 Size: 1077 5065 SbfI CC_TGCA'GG Cuts at: 2487 2832 3494 2487 Size: 345 662 5135 Fragments arranged by size: 5135 662 345 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2483 2828 3341 3490 4625 5306 5497 Size: 219 1438 345 513 149 1135 681 191 Cuts at: 5497 826 Size: 1471 Fragments arranged by size: 1471 1438 1135 681 513 345 219 191 149 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 2419 1700 Size: 719 5423 SmaI CCC'GGG Cuts at: 1872 2016 2201 3379 3469 1872 Size: 144 185 1178 90 4545 Fragments arranged by size: 4545 1178 185 144 90 SmlI C'TyrA_G Cuts at: 820 1017 1943 2603 2633 4246 5147 5409 820 Size: 197 926 660 30 1613 901 262 1553 Fragments arranged by size: 1613 1553 926 901 660 262 197 30 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 2432 3500 1132 Size: 1300 1068 3774 Fragments arranged by size: 3774 1300 1068 SrfI GCCC'GGGC Cuts at: 2016 2016 Size: 6142 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2372 2412 2631 2834 1517 Size: 855 40 219 203 4825 Fragments arranged by size: 4825 855 219 203 40 StyI C'CwwG_G Cuts at: 513 1065 2112 2253 2433 2997 3581 513 Size: 552 1047 141 180 564 584 3074 Fragments arranged by size: 3074 1047 584 564 552 180 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 2176 2191 2283 4169 4186 4339 Size: 1255 504 92 15 92 1886 17 153 Cuts at: 4339 4524 4969 325 Size: 185 445 1498 Fragments arranged by size: 1886 1498 1255 504 445 185 153 92 92 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4388 6100 96 Size: 274 80 33 51 1443 2411 1712 138 Fragments arranged by size: 2411 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2151 2183 6102 6134 2151 Size: 32 3919 32 2159 Fragments arranged by size: 3919 2159 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2935 2935 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 2633 1943 Size: 690 5452 XmaI C'CCGG_G Cuts at: 1870 2014 2199 3377 3467 1870 Size: 144 185 1178 90 4545 Fragments arranged by size: 4545 1178 185 144 90 XmnI GAAnn'nnTTC Cuts at: 2471 4271 2471 Size: 1800 4342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI