(Linear) (Six-base) MAPSORT of: KIEE4109.seq Check: 9947 from: 1 to: 1437 KIEE4109.orf With 182 enzymes: SgfI * March 22, 2012 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 220 1421 1437 Size: 220 1201 16 Fragments arranged by size: 1201 220 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1264 1437 Size: 1264 173 AfeI AGC'GCT Cuts at: 0 604 1437 Size: 604 833 AleI CACnn'nnGTG Cuts at: 0 415 1437 Size: 415 1022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 503 1437 Size: 503 934 AlwNI CAG_nnn'CTG Cuts at: 0 798 1344 1413 1437 Size: 798 546 69 24 Fragments arranged by size: 798 546 69 24 ApaI G_GGCC'C Cuts at: 0 217 1437 Size: 217 1220 ApaLI G'TGCA_C Cuts at: 0 219 946 1437 Size: 219 727 491 Fragments arranged by size: 727 491 219 AscI GG'CGCG_CC Cuts at: 0 868 1437 Size: 868 569 AvaI C'yCGr_G Cuts at: 0 9 194 628 981 1372 1384 1437 Size: 9 185 434 353 391 12 53 Fragments arranged by size: 434 391 353 185 53 12 9 BanI G'GyrC_C Cuts at: 0 252 260 1064 1437 Size: 252 8 804 373 Fragments arranged by size: 804 373 252 8 BanII G_rGCy'C Cuts at: 0 10 217 314 437 1019 1130 1391 1437 Size: 10 207 97 123 582 111 261 46 Fragments arranged by size: 582 261 207 123 111 97 46 10 BbvCI CC'TCA_GC Cuts at: 0 243 1168 1437 Size: 243 925 269 Fragments arranged by size: 925 269 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1202 1236 1437 Size: 1202 34 201 Fragments arranged by size: 1202 201 34 BglI GCCn_nnn'nGGC Cuts at: 0 521 781 983 1437 Size: 521 260 202 454 Fragments arranged by size: 521 454 260 202 BlpI GC'TnA_GC Cuts at: 0 739 817 973 1437 Size: 739 78 156 464 Fragments arranged by size: 739 464 156 78 Bme1580I G_kGCm'C Cuts at: 0 115 217 223 263 950 1069 1437 Size: 115 102 6 40 687 119 368 Fragments arranged by size: 687 368 119 115 102 40 6 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 1111 1366 1375 1437 Size: 688 423 255 9 62 Fragments arranged by size: 688 423 255 62 9 Bpu10I CC'TnA_GC Cuts at: 0 243 718 1168 1437 Size: 243 475 450 269 Fragments arranged by size: 475 450 269 243 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 1437 Size: 583 854 BsaAI yAC'GTr Cuts at: 0 837 1098 1437 Size: 837 261 339 Fragments arranged by size: 837 339 261 BsaHI Gr'CG_yC Cuts at: 0 524 773 1437 Size: 524 249 664 Fragments arranged by size: 664 524 249 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 146 176 1437 Size: 146 30 1261 Fragments arranged by size: 1261 146 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1138 1363 1437 Size: 1138 225 74 Fragments arranged by size: 1138 225 74 BseYI C'CCAG_C Cuts at: 0 172 208 264 911 1437 Size: 172 36 56 647 526 Fragments arranged by size: 647 526 172 56 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1096 1351 1437 Size: 1096 255 86 Fragments arranged by size: 1096 255 86 BsiEI CG_ry'CG Cuts at: 0 277 1437 Size: 277 1160 BsiHKAI G_wGCw'C Cuts at: 0 223 437 950 1019 1130 1136 1391 1437 Size: 223 214 513 69 111 6 255 46 Fragments arranged by size: 513 255 223 214 111 69 46 6 BsmBI CGTCTCn'nnnn_ Cuts at: 0 531 1437 Size: 531 906 Bsp1286I G_dGCh'C Cuts at: 0 10 115 217 223 263 314 437 950 Size: 10 105 102 6 40 51 123 513 Cuts at: 950 1019 1069 1130 1136 1391 1437 Size: 69 50 61 6 255 46 Fragments arranged by size: 513 255 123 105 102 69 61 51 50 46 40 10 6 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 220 485 1040 1118 1217 1421 1437 Size: 220 265 555 78 99 204 16 Fragments arranged by size: 555 265 220 204 99 78 16 BsrBI CCG'CTC Cuts at: 0 273 1437 Size: 273 1164 BsrFI r'CCGG_y Cuts at: 0 414 1437 Size: 414 1023 BssHII G'CGCG_C Cuts at: 0 722 724 868 1437 Size: 722 2 144 569 Fragments arranged by size: 722 569 144 2 BssSI C'ACGA_G Cuts at: 0 1018 1059 1129 1437 Size: 1018 41 70 308 Fragments arranged by size: 1018 308 70 41 BstAPI GCAn_nnn'nTGC Cuts at: 0 798 894 954 1413 1437 Size: 798 96 60 459 24 Fragments arranged by size: 798 459 96 60 24 BstEII G'GTnAC_C Cuts at: 0 996 1437 Size: 996 441 BstXI CCAn_nnnn'nTGG Cuts at: 0 87 1437 Size: 87 1350 BstYI r'GATC_y Cuts at: 0 299 1437 Size: 299 1138 BtgI C'CryG_G Cuts at: 0 287 927 1437 Size: 287 640 510 Fragments arranged by size: 640 510 287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 762 1227 1437 Size: 762 465 210 Fragments arranged by size: 762 465 210 DraIII CAC_nnn'GTG Cuts at: 0 933 1437 Size: 933 504 EaeI y'GGCC_r Cuts at: 0 95 274 284 764 782 1022 1437 Size: 95 179 10 480 18 240 415 Fragments arranged by size: 480 415 240 179 95 18 10 EagI C'GGCC_G Cuts at: 0 274 1437 Size: 274 1163 EarI CTCTTCn'nnn_ Cuts at: 0 319 1396 1437 Size: 319 1077 41 Fragments arranged by size: 1077 319 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 826 1437 Size: 826 611 EcoICRI GAG'CTC Cuts at: 0 435 1017 1128 1389 1437 Size: 435 582 111 261 48 Fragments arranged by size: 582 435 261 111 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 1111 1264 1366 1375 1437 Size: 688 423 153 102 9 62 Fragments arranged by size: 688 423 153 102 62 9 EcoO109I rG'GnC_Cy Cuts at: 0 213 1437 Size: 213 1224 FspI TGC'GCA Cuts at: 0 1036 1045 1437 Size: 1036 9 392 Fragments arranged by size: 1036 392 9 HaeII r_GCGC'y Cuts at: 0 606 853 1090 1437 Size: 606 247 237 347 Fragments arranged by size: 606 347 247 237 HincII GTy'rAC Cuts at: 0 939 1437 Size: 939 498 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 554 1437 Size: 554 883 MscI TGG'CCA Cuts at: 0 286 1437 Size: 286 1151 MslI CAynn'nnrTG Cuts at: 0 415 760 1081 1437 Size: 415 345 321 356 Fragments arranged by size: 415 356 345 321 MspA1I CmG'CkG Cuts at: 0 268 282 795 1299 1335 1421 1437 Size: 268 14 513 504 36 86 16 Fragments arranged by size: 513 504 268 86 36 16 14 NotI GC'GGCC_GC Cuts at: 0 274 1437 Size: 274 1163 NspI r_CATG'y Cuts at: 0 427 1437 Size: 427 1010 PasI CC'CwG_GG Cuts at: 0 1260 1437 Size: 1260 177 PflMI CCAn_nnn'nTGG Cuts at: 0 398 933 1437 Size: 398 535 504 Fragments arranged by size: 535 504 398 PmlI CAC'GTG Cuts at: 0 837 1437 Size: 837 600 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 451 483 1437 Size: 451 32 954 Fragments arranged by size: 954 451 32 PspOMI G'GGCC_C Cuts at: 0 213 1437 Size: 213 1224 PspXI vC'TCGA_Gb Cuts at: 0 628 1437 Size: 628 809 PstI C_TGCA'G Cuts at: 0 482 827 1340 1437 Size: 482 345 513 97 Fragments arranged by size: 513 482 345 97 PvuII CAG'CTG Cuts at: 0 795 1335 1437 Size: 795 540 102 Fragments arranged by size: 795 540 102 SacI G_AGCT'C Cuts at: 0 437 1019 1130 1391 1437 Size: 437 582 111 261 46 Fragments arranged by size: 582 437 261 111 46 SapI GCTCTTCn'nnn_ Cuts at: 0 319 1396 1437 Size: 319 1077 41 Fragments arranged by size: 1077 319 41 SbfI CC_TGCA'GG Cuts at: 0 482 827 1437 Size: 482 345 610 Fragments arranged by size: 610 482 345 SfcI C'TryA_G Cuts at: 0 478 823 1336 1437 Size: 478 345 513 101 Fragments arranged by size: 513 478 345 101 SgrAI Cr'CCGG_yG Cuts at: 0 414 1437 Size: 414 1023 SmaI CCC'GGG Cuts at: 0 11 196 1374 1437 Size: 11 185 1178 63 Fragments arranged by size: 1178 185 63 11 SmlI C'TyrA_G Cuts at: 0 598 628 1437 Size: 598 30 809 Fragments arranged by size: 809 598 30 SphI G_CATG'C Cuts at: 0 427 1437 Size: 427 1010 SrfI GCCC'GGGC Cuts at: 0 11 1437 Size: 11 1426 StuI AGG'CCT Cuts at: 0 367 407 626 829 1437 Size: 367 40 219 203 608 Fragments arranged by size: 608 367 219 203 40 StyI C'CwwG_G Cuts at: 0 107 248 428 992 1437 Size: 107 141 180 564 445 Fragments arranged by size: 564 445 180 141 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 79 171 186 278 1437 Size: 79 92 15 92 1159 Fragments arranged by size: 1159 92 92 79 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 146 178 1437 Size: 146 32 1259 Fragments arranged by size: 1259 146 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 930 1437 Size: 930 507 XhoI C'TCGA_G Cuts at: 0 628 1437 Size: 628 809 XmaI C'CCGG_G Cuts at: 0 9 194 1372 1437 Size: 9 185 1178 65 Fragments arranged by size: 1178 185 65 9 XmnI GAAnn'nnTTC Cuts at: 0 466 1437 Size: 466 971 Enzymes that do cut: AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbvCI BcgI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII HincII MmeI MscI MslI MspA1I NotI NspI PasI PflMI PmlI PpiI PspOMI PspXI PstI PvuII SacI SapI SbfI SfcI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AloI ApoI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI PfoI PmeI PpuMI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SspI SwaI TatI Tth111I XbaI ZraI