################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AflIII 206 ACACGT A^C[GA][CT]GT Alw44I 616 GTGCAC G^TGCAC ApaLI 616 GTGCAC G^TGCAC BamHI 266 GGATCC G^GATCC BcgI 336 CGATGAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 730 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 18 TCCGGT [AT]^CCGG[AT] BsmAI 601 GTCTC GTCTC(1/5) BsmBI 600 CGTCTC CGTCTC(1/5) BspDI 334 ATCGAT AT^CGAT BspHI 701 TCATGA T^CATGA BstXI 164 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 334 ATCGAT AT^CGAT DdeI 260 CTCAG C^T[ACGT]AG EarI 24 GAAGAG CTCTTC(1/4) EcoNI 245 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 256 GGCGCT [GA]GCGC^[CT] HindIII 631 AAGCTT A^AGCTT Ksp632I 24 GAAGAG CTCTTC(1/4) MslI 609 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 179 GCCGGC GCC^GGC NgoMIV 179 GCCGGC G^CCGGC NspI 342 GCATGC [GA]CATG^[CT] PflMI 290 CCAACCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 701 TCATGA T^CATGA SacI 196 GAGCTC GAGCT^C SapI 24 GAAGAGC GCTCTTC(1/4) SfiI 729 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 342 GCATGC GCATG^C Tsp45I 646 GTCAC ^GT[GC]AC TspRI 638 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 290 CCAACCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 161 CCACCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 126 GTAC GT^AC 429 GTAC BalI 680 TGGCCA TGG^CCA 728 TGGCCA BglII 545 AGATCT A^GATCT 836 AGATCT BsiWI 125 CGTACG C^GTACG 428 CGTACG BspMI 38 ACCTGC ACCTGC(4/8) 402 GCAGGT BstF5I 165 CATCC GGATG(2/0) 786 CATCC EagI 177 CGGCCG C^GGCCG 711 CGGCCG EclXI 177 CGGCCG C^GGCCG 711 CGGCCG Eco52I 177 CGGCCG C^GGCCG 711 CGGCCG FokI 165 CATCC GGATG(9/13) 786 CATCC MluNI 680 TGGCCA TGG^CCA 728 TGGCCA MscI 680 TGGCCA TGG^CCA 728 TGGCCA PstI 100 CTGCAG CTGCA^G 574 CTGCAG RsaI 126 GTAC GT^AC 429 GTAC SbfI 99 CCTGCAGG CCTGCA^GG 573 CCTGCAGG Sse8387I 99 CCTGCAGG CCTGCA^GG 573 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI TthHB8I XhoII