Restriction map for
pFN21AE0909
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BseRI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MaeII MamI MfeI MroI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 490 GTCTAC GT^[AC][GT]AC
AcyI 147 GGCGCC G[GA]^CG[CT]C
AflIII 14 ACGCGT A^C[GA][CT]GT
AlwNI 34 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 206 TGGCCA TGG^CCA
BanI 147 GGCGCC G^G[CT][GA]CC
BbeI 147 GGCGCC GGCGC^C
BglII 401 AGATCT A^GATCT
BpmI 40 CTGGAG CTGGAG(16/14)
BsaHI 147 GGCGCC G[GA]^CG[CT]C
BsaI 646 GGTCTC GGTCTC(1/5)
BsaWI 543 TCCGGT [AT]^CCGG[AT]
BsiEI 245 CGGCCG CG[GA][CT]^CG
BsmAI 647 GTCTC GTCTC(1/5)
BspMI 273 GCAGGT ACCTGC(4/8)
BsrI 665 ACTGG ACTGG(1/-1)
BstEII 276 GGTGACC G^GT[ACGT]ACC
BstF5I 123 GGATG GGATG(2/0)
BstPI 276 GGTGACC G^GT[ACGT]ACC
DsaI 248 CCGTGG C^C[GA][CT]GG
EagI 245 CGGCCG C^GGCCG
EclXI 245 CGGCCG C^GGCCG
Eco52I 245 CGGCCG C^GGCCG
EcoO65I 276 GGTGACC G^GT[ACGT]ACC
FokI 123 GGATG GGATG(9/13)
Hin1I 147 GGCGCC G[GA]^CG[CT]C
KasI 147 GGCGCC G^GCGCC
MluI 14 ACGCGT A^CGCGT
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
NaeI 102 GCCGGC GCC^GGC
NarI 147 GGCGCC GG^CGCC
NgoMIV 102 GCCGGC G^CCGGC
PpuMI 338 GGGACCT [GA]G^G[AT]CC[CT]
SapI 159 GAAGAGC GCTCTTC(1/4)
SexAI 359 ACCAGGT A^CC[AT]GGT
SfaNI 653 GCATC GCATC(5/9)
SfoI 147 GGCGCC GGC^GCC
SmlI 439 CTCAAG C^T[CT][GA]AG
TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 15 CGCG CG^CG
475 CGCG
AfaI 141 GTAC GT^AC
213 GTAC
AlwI 174 GATCC GGATC(4/5)
327 GGATC
BsgI 448 CTGCAC GTGCAG(16/14)
271 GTGCAG
BsmFI 338 GGGAC GGGAC(10/14)
597 GTCCC
BsrFI 102 GCCGGC [GA]^CCGG[CT]
243 ACCGGC
BstUI 15 CGCG CG^CG
475 CGCG
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
243 ACCGGC
DrdI 288 GACACTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
481 GACAAGACCGTC
EaeI 206 TGGCCA [CT]^GGCC[GA]
245 CGGCCG
EarI 601 CTCTTC CTCTTC(1/4)
159 GAAGAG
FauI 219 GCGGG CCCGC(4/6)
576 GCGGG
HgaI 13 GACGC GACGC(5/10)
621 GACGC
HinfI 71 GAGTC G^A[ACGT]TC
540 GACTC
HphI 276 GGTGA GGTGA(8/7)
605 TCACC
Ksp632I 601 CTCTTC CTCTTC(1/4)
159 GAAGAG
MaeIII 277 GTGAC ^GT[ACGT]AC
538 GTGAC
MvnI 15 CGCG CG^CG
475 CGCG
NlaIII 324 CATG CATG^
495 CATG
PleI 540 GACTC GAGTC(4/5)
71 GAGTC
PvuII 34 CAGCTG CAG^CTG
181 CAGCTG
RsaI 141 GTAC GT^AC
213 GTAC
Tsp45I 277 GTGAC ^GT[GC]AC
538 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI ApaI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsoBI Bsp1286I BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI XhoII