(Circular) (Six-base) MAPSORT of: pFN21AE0909.seq Check: 8228 from: 1 to: 5380 pFN21AE0909 5380 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2269 2269 Size: 5380 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3190 278 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2496 2723 1937 Size: 559 227 4594 Fragments arranged by size: 4594 559 227 Acc65I G'GTAC_C Cuts at: 2701 2701 Size: 5380 AclI AA'CG_TT Cuts at: 1563 3507 3880 1563 Size: 1944 373 3063 Fragments arranged by size: 3063 1944 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2399 3443 4827 5095 1632 Size: 746 21 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 746 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5183 AflIII A'CryG_T Cuts at: 1203 2019 4279 5256 1203 Size: 816 2260 977 1327 Fragments arranged by size: 2260 1327 977 816 AgeI A'CCGG_T Cuts at: 4211 4211 Size: 5380 AhdI GACnn_n'nnGTC Cuts at: 4109 4109 Size: 5380 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5380 AlwNI CAG_nnn'CTG Cuts at: 2044 3123 4695 2044 Size: 1079 1572 2729 Fragments arranged by size: 2729 1572 1079 ApaI G_GGCC'C Cuts at: 2241 2322 2588 2241 Size: 81 266 5033 Fragments arranged by size: 5033 266 81 ApaLI G'TGCA_C Cuts at: 3437 4593 3437 Size: 1156 4224 ApoI r'AATT_y Cuts at: 1493 2689 2915 3067 5176 1493 Size: 1196 226 152 2109 1697 Fragments arranged by size: 2109 1697 1196 226 152 AseI AT'TA_AT Cuts at: 160 1032 3934 160 Size: 872 2902 1606 Fragments arranged by size: 2902 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2141 2518 2705 1870 Size: 73 130 68 377 187 4545 Fragments arranged by size: 4545 377 187 130 73 68 BamHI G'GATC_C Cuts at: 2710 3077 2710 Size: 367 5013 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 2701 4156 618 Size: 325 217 627 365 549 1455 1842 Fragments arranged by size: 1842 1455 627 549 365 325 217 BanII G_rGCy'C Cuts at: 1391 2241 2322 2588 2699 1391 Size: 850 81 266 111 4072 Fragments arranged by size: 4072 850 266 111 81 BbeI G_GCGC'C Cuts at: 2156 2156 Size: 5380 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3571 3605 1903 Size: 34 50 34 1550 34 3678 Fragments arranged by size: 3678 1550 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3272 4482 3272 Size: 1210 4170 BclI T'GATC_A Cuts at: 1548 1548 Size: 5380 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3991 136 Size: 107 122 71 631 605 2319 1525 Fragments arranged by size: 2319 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2406 5375 2406 Size: 2969 2411 BlpI GC'TnA_GC Cuts at: 2797 2797 Size: 5380 Bme1580I G_kGCm'C Cuts at: 1790 2142 2241 2322 2588 3441 4597 1790 Size: 352 99 81 266 853 1156 2573 Fragments arranged by size: 2573 1156 853 352 266 99 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5380 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 4069 442 Size: 416 53 83 399 24 1694 958 1753 Fragments arranged by size: 1753 1694 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 4040 1239 Size: 827 1974 2579 Fragments arranged by size: 2579 1974 827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2429 3505 4370 4668 2429 Size: 1076 865 298 3141 Fragments arranged by size: 3141 1076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4043 882 Size: 617 1158 1386 2219 Fragments arranged by size: 2219 1386 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 3171 493 Size: 711 1967 2702 Fragments arranged by size: 2702 1967 711 BsaBI GATnn'nnATC Cuts at: 5374 5374 Size: 5380 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2153 3187 3569 4204 Size: 53 83 186 936 620 1034 382 635 Cuts at: 4204 275 Size: 1451 Fragments arranged by size: 1451 1034 936 635 620 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2548 3813 4211 4485 4632 5043 2548 Size: 1265 398 274 147 411 2885 Fragments arranged by size: 2885 1265 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2522 2552 1178 Size: 30 165 30 1119 30 4006 Fragments arranged by size: 4006 1119 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5380 BseYI C'CCAG_C Cuts at: 2008 2029 2233 4583 5079 2008 Size: 21 204 2350 496 2309 Fragments arranged by size: 2350 2309 496 204 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2438 1163 Size: 573 561 141 4105 Fragments arranged by size: 4105 573 561 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 2830 3591 4619 1540 Size: 275 186 252 577 761 1028 2301 Fragments arranged by size: 2301 1028 761 577 275 252 186 BsiHKAI G_wGCw'C Cuts at: 3441 3526 4597 3441 Size: 85 1071 4224 Fragments arranged by size: 4224 1071 85 BsmI GAATG_Cn' Cuts at: 2891 2984 2891 Size: 93 5287 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2241 2322 2588 2699 3441 3526 Size: 399 352 99 81 266 111 742 85 Cuts at: 3526 4597 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 266 111 99 85 81 BspEI T'CCGG_A Cuts at: 5043 5043 Size: 5380 BspHI T'CATG_A Cuts at: 3269 4999 3269 Size: 1730 3650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2269 2735 844 Size: 1425 466 3489 Fragments arranged by size: 3489 1425 466 BsrBI CCG'CTC Cuts at: 1394 1823 3267 1394 Size: 429 1444 3507 Fragments arranged by size: 3507 1444 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3875 4049 65 Size: 1183 2627 174 1396 Fragments arranged by size: 2627 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2248 4024 4211 1700 Size: 110 297 141 1776 187 2869 Fragments arranged by size: 2869 1776 297 187 141 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5380 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5380 BssSI C'ACGA_G Cuts at: 3440 4452 3440 Size: 1012 4368 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5380 BstBI TT'CG_AA Cuts at: 4184 4184 Size: 5380 BstEII G'GTnAC_C Cuts at: 1184 2281 1184 Size: 1097 4283 BstYI r'GATC_y Cuts at: 1972 2178 2332 2406 2710 3077 3462 3479 4931 Size: 206 154 74 304 367 385 17 1452 Cuts at: 4931 5017 5029 5375 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 304 206 154 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2253 513 Size: 552 1188 3640 Fragments arranged by size: 3640 1188 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4641 Fragments arranged by size: 4641 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2896 3690 3710 2118 Size: 778 794 20 3788 Fragments arranged by size: 3788 794 778 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2080 2115 529 Size: 35 1516 35 3794 Fragments arranged by size: 3794 1516 35 35 DraI TTT'AAA Cuts at: 2684 2684 Size: 5380 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2299 2492 4387 809 Size: 507 983 193 1895 1802 Fragments arranged by size: 1895 1802 983 507 193 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2250 2827 Size: 54 1425 50 275 18 381 39 577 Cuts at: 2827 3716 5345 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 577 381 275 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 2827 1537 Size: 275 438 577 4090 Fragments arranged by size: 4090 577 438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2159 2612 3310 1353 Size: 806 453 698 3423 Fragments arranged by size: 3423 806 698 453 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3967 4341 4487 387 Size: 1064 138 2378 374 146 1280 Fragments arranged by size: 2378 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2378 2399 3443 4040 4827 5095 Size: 393 434 312 21 1044 597 787 268 Cuts at: 5095 1239 Size: 1524 Fragments arranged by size: 1524 1044 787 597 434 393 312 268 21 EcoO109I rG'GnC_Cy Cuts at: 2237 2238 2318 2344 2473 2584 2585 2237 Size: 1 80 26 129 111 1 5032 Fragments arranged by size: 5032 129 111 80 26 1 1 EcoRI G'AATT_C Cuts at: 2689 3067 2689 Size: 378 5002 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2428 2188 Size: 240 5140 FspI TGC'GCA Cuts at: 3886 3886 Size: 5380 HaeII r_GCGC'y Cuts at: 1678 2066 2156 2526 2564 4527 1678 Size: 388 90 370 38 1963 2531 Fragments arranged by size: 2531 1963 388 370 90 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2391 2423 4021 4053 4095 Size: 32 135 32 819 32 1598 32 42 Cuts at: 4095 4127 1373 Size: 32 2626 Fragments arranged by size: 2626 1598 819 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2724 2970 669 Size: 988 281 786 246 3079 Fragments arranged by size: 3079 988 786 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5380 HpaI GTT'AAC Cuts at: 2970 2970 Size: 5380 KasI G'GCGC_C Cuts at: 2152 2152 Size: 5380 KpnI G_GTAC'C Cuts at: 2705 2705 Size: 5380 MfeI C'AATT_G Cuts at: 2979 2979 Size: 5380 MluI A'CGCG_T Cuts at: 2019 5256 2019 Size: 3237 2143 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 2283 4494 4678 1305 Size: 801 177 2211 184 2007 Fragments arranged by size: 2211 2007 801 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 10 Size: 54 1425 343 381 3177 Fragments arranged by size: 3177 1425 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3338 3697 3856 518 Size: 648 2172 359 159 2042 Fragments arranged by size: 2172 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2188 2439 2796 3473 4621 4866 1606 Size: 435 147 251 357 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 435 357 251 245 147 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5380 NarI GG'CG_CC Cuts at: 2153 2153 Size: 5380 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4828 NdeI CA'TA_TG Cuts at: 387 387 Size: 5380 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5380 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5380 NotI GC'GGCC_GC Cuts at: 2827 2827 Size: 5380 NspI r_CATG'y Cuts at: 1132 1229 2738 4283 1132 Size: 97 1509 1545 2229 Fragments arranged by size: 2229 1545 1509 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5380 PciI A'CATG_T Cuts at: 4279 4279 Size: 5380 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5380 PmeI GTTT'AAAC Cuts at: 2684 2684 Size: 5380 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 4962 4994 3449 Size: 32 1481 32 3835 Fragments arranged by size: 3835 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2344 2344 Size: 5380 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5380 PsiI TTA'TAA Cuts at: 2950 2950 Size: 5380 PspOMI G'GGCC_C Cuts at: 2237 2318 2584 2237 Size: 81 266 5033 Fragments arranged by size: 5033 266 81 PstI C_TGCA'G Cuts at: 830 2732 830 Size: 1902 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5380 PvuII CAG'CTG Cuts at: 2041 2188 2041 Size: 147 5233 SalI G'TCGA_C Cuts at: 1936 2722 1936 Size: 786 4594 SapI GCTCTTCn'nnn_ Cuts at: 2159 2159 Size: 5380 SbfI CC_TGCA'GG Cuts at: 2732 2732 Size: 5380 ScaI AGT'ACT Cuts at: 3628 5340 3628 Size: 1712 3668 SexAI A'CCwGG_T Cuts at: 2364 2364 Size: 5380 SfcI C'TryA_G Cuts at: 826 1045 2728 3863 4544 4735 826 Size: 219 1683 1135 681 191 1471 Fragments arranged by size: 1683 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2154 2154 Size: 5380 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5380 SmaI CCC'GGG Cuts at: 1872 2707 1872 Size: 835 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 3484 4385 4647 820 Size: 197 926 501 1040 901 262 1553 Fragments arranged by size: 1553 1040 926 901 501 262 197 SnaBI TAC'GTA Cuts at: 493 3171 493 Size: 2678 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5380 SphI G_CATG'C Cuts at: 1132 2738 1132 Size: 1606 3774 SspI AAT'ATT Cuts at: 5 52 3304 5 Size: 47 3252 2081 Fragments arranged by size: 3252 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5380 StyI C'CwwG_G Cuts at: 513 1065 2819 513 Size: 552 1754 3074 Fragments arranged by size: 3074 1754 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3407 3424 3577 3762 4207 325 Size: 1255 1827 17 153 185 445 1498 Fragments arranged by size: 1827 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 3626 5338 Size: 274 80 33 51 1443 240 1409 1712 Cuts at: 5338 96 Size: 138 Fragments arranged by size: 1712 1443 1409 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2621 2653 5340 5372 2621 Size: 32 2687 32 2629 Fragments arranged by size: 2687 2629 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5380 XbaI T'CTAG_A Cuts at: 2716 2716 Size: 5380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5380 XmaI C'CCGG_G Cuts at: 1870 2705 1870 Size: 835 4545 XmnI GAAnn'nnTTC Cuts at: 3509 3509 Size: 5380 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3188 276 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI