Restriction map for
pFN21AE0909
(Linear) (Six-base) MAPSORT of: KIEE0909.seq Check: 4888 from: 1 to: 675
KIEE0909.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 264 675
Size: 264 411
AccI GT'mk_AC
Cuts at: 0 491 675
Size: 491 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 394 675
Size: 373 21 281
Fragments arranged by size:
373 281 21
AflIII A'CryG_T
Cuts at: 0 14 675
Size: 14 661
AlwNI CAG_nnn'CTG
Cuts at: 0 39 675
Size: 39 636
ApaI G_GGCC'C
Cuts at: 0 236 317 583 675
Size: 236 81 266 92
Fragments arranged by size:
266 236 92 81
AvaI C'yCGr_G
Cuts at: 0 68 136 513 675
Size: 68 68 377 162
Fragments arranged by size:
377 162 68 68
BanI G'GyrC_C
Cuts at: 0 147 675
Size: 147 528
BanII G_rGCy'C
Cuts at: 0 236 317 583 675
Size: 236 81 266 92
Fragments arranged by size:
266 236 92 81
BbeI G_GCGC'C
Cuts at: 0 151 675
Size: 151 524
BglII A'GATC_T
Cuts at: 0 401 675
Size: 401 274
Bme1580I G_kGCm'C
Cuts at: 0 137 236 317 583 675
Size: 137 99 81 266 92
Fragments arranged by size:
266 137 99 92 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 675
Size: 61 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 675
Size: 424 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 675
Size: 652 23
BsaHI Gr'CG_yC
Cuts at: 0 148 675
Size: 148 527
BsaWI w'CCGG_w
Cuts at: 0 543 675
Size: 543 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 675
Size: 517 30 128
Fragments arranged by size:
517 128 30
BseYI C'CCAG_C
Cuts at: 0 3 24 228 675
Size: 3 21 204 447
Fragments arranged by size:
447 204 21 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 433 675
Size: 292 141 242
Fragments arranged by size:
292 242 141
BsiEI CG_ry'CG
Cuts at: 0 248 675
Size: 248 427
Bsp1286I G_dGCh'C
Cuts at: 0 137 236 317 583 675
Size: 137 99 81 266 92
Fragments arranged by size:
266 137 99 92 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 264 675
Size: 264 411
BsrFI r'CCGG_y
Cuts at: 0 102 243 675
Size: 102 141 432
Fragments arranged by size:
432 141 102
BstEII G'GTnAC_C
Cuts at: 0 276 675
Size: 276 399
BstYI r'GATC_y
Cuts at: 0 173 327 401 675
Size: 173 154 74 274
Fragments arranged by size:
274 173 154 74
BtgI C'CryG_G
Cuts at: 0 248 675
Size: 248 427
BtsI GCAGTG_nn'
Cuts at: 0 113 675
Size: 113 562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 75 110 675
Size: 75 35 565
Fragments arranged by size:
565 75 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 294 487 675
Size: 294 193 188
Fragments arranged by size:
294 193 188
EaeI y'GGCC_r
Cuts at: 0 206 245 675
Size: 206 39 430
Fragments arranged by size:
430 206 39
EagI C'GGCC_G
Cuts at: 0 245 675
Size: 245 430
EarI CTCTTCn'nnn_
Cuts at: 0 154 607 675
Size: 154 453 68
Fragments arranged by size:
453 154 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 373 394 675
Size: 61 312 21 281
Fragments arranged by size:
312 281 61 21
EcoO109I rG'GnC_Cy
Cuts at: 0 232 233 313 339 468 579 580 675
Size: 232 1 80 26 129 111 1 95
Fragments arranged by size:
232 129 111 95 80 26 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 423 675
Size: 183 240 252
Fragments arranged by size:
252 240 183
HaeII r_GCGC'y
Cuts at: 0 61 151 521 559 675
Size: 61 90 370 38 116
Fragments arranged by size:
370 116 90 61 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 386 418 675
Size: 386 32 257
Fragments arranged by size:
386 257 32
KasI G'GCGC_C
Cuts at: 0 147 675
Size: 147 528
MluI A'CGCG_T
Cuts at: 0 14 675
Size: 14 661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 278 675
Size: 101 177 397
Fragments arranged by size:
397 177 101
MscI TGG'CCA
Cuts at: 0 208 675
Size: 208 467
MspA1I CmG'CkG
Cuts at: 0 36 183 434 675
Size: 36 147 251 241
Fragments arranged by size:
251 241 147 36
NaeI GCC'GGC
Cuts at: 0 104 675
Size: 104 571
NarI GG'CG_CC
Cuts at: 0 148 675
Size: 148 527
NgoMIV G'CCGG_C
Cuts at: 0 102 675
Size: 102 573
PpuMI rG'GwC_Cy
Cuts at: 0 339 675
Size: 339 336
PspOMI G'GGCC_C
Cuts at: 0 232 313 579 675
Size: 232 81 266 96
Fragments arranged by size:
266 232 96 81
PvuII CAG'CTG
Cuts at: 0 36 183 675
Size: 36 147 492
Fragments arranged by size:
492 147 36
SapI GCTCTTCn'nnn_
Cuts at: 0 154 675
Size: 154 521
SexAI A'CCwGG_T
Cuts at: 0 359 675
Size: 359 316
SfoI GGC'GCC
Cuts at: 0 149 675
Size: 149 526
SmlI C'TyrA_G
Cuts at: 0 439 675
Size: 439 236
TatI w'GTAC_w
Cuts at: 0 212 675
Size: 212 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 616 648 675
Size: 616 32 27
Fragments arranged by size:
616 32 27
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI AvaI BanI
BanII BbeI BglII Bme1580I BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI Bsp1286I BspMI BsrFI
BstEII BstYI BtgI BtsI CspCI DrdI EaeI EagI
EarI Eco57MI EcoO109I FalI HaeII Hin4I KasI MluI
MmeI MscI MspA1I NaeI NarI NgoMIV PpuMI PspOMI
PvuII SapI SexAI SfoI SmlI TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI
BsaBI BseRI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI