Restriction map for
pFN21AE6184
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstF5I BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HinfI HpaI HphI
KasI KpnI Ksp632I MaeI MamI
MboI MfeI MflI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AccI 70 GTCTAC GT^[AC][GT]AC
AfaI 420 GTAC GT^AC
AflIII 263 ACGCGT A^C[GA][CT]GT
AlwNI 298 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 120 GAAGAC GAAGAC(2/6)
BcgI 56 GCAGCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 120 GAAGAC GAAGAC(2/6)
BseRI 156 CTCCTC GAGGAG(10/8)
BsgI 259 CTGCAC GTGCAG(16/14)
BsmAI 347 GAGAC GTCTC(1/5)
BspMI 257 ACCTGC ACCTGC(4/8)
BssHII 33 GCGCGC G^CGCGC
BstEII 321 GGTAACC G^GT[ACGT]ACC
BstPI 321 GGTAACC G^GT[ACGT]ACC
EcoO65I 321 GGTAACC G^GT[ACGT]ACC
KspI 11 CCGCGG CCGC^GG
MaeIII 322 GTAAC ^GT[ACGT]AC
MluI 263 ACGCGT A^CGCGT
PpuMI 94 AGGACCT [GA]G^G[AT]CC[CT]
PstI 364 CTGCAG CTGCA^G
PvuII 271 CAGCTG CAG^CTG
RsaI 420 GTAC GT^AC
SacII 11 CCGCGG CCGC^GG
SfcI 364 CTGCAG C^T[GA][CT]AG
TspRI 310 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcyI 67 GGCGTC G[GA]^CG[CT]C
369 GGCGTC
BanII 147 GAGCCC G[GA]GC[CT]^C
170 GAGCCC
BlpI 198 GCTGAGC GC^T[ACGT]AGC
249 GCTGAGC
BmyI 147 GAGCCC G[AGT]GC[ACT]^C
170 GAGCCC
Bpu1102I 198 GCTGAGC GC^T[ACGT]AGC
249 GCTGAGC
BsaHI 67 GGCGTC G[GA]^CG[CT]C
369 GGCGTC
Bsp1286I 147 GAGCCC G[AGT]GC[ACT]^C
170 GAGCCC
CelII 198 GCTGAGC GC^T[ACGT]AGC
249 GCTGAGC
DraII 83 AGGCCCT [GA]G^G[ACGT]CC[CT]
94 AGGACCT
EaeI 177 CGGCCA [CT]^GGCC[GA]
287 CGGCCA
EcoO109I 83 AGGCCCT [GA]G^G[ACGT]CC[CT]
94 AGGACCT
FauI 112 CCCGC CCCGC(4/6)
358 CCCGC
Hin1I 67 GGCGTC G[GA]^CG[CT]C
369 GGCGTC
MaeII 62 ACGT A^CGT
395 ACGT
MboII 45 GAAGA GAAGA(8/7)
120 GAAGA
MspA1I 11 CCGCGG C[AC]G^C[GT]G
271 CAGCTG
NlaIV 154 GGCTCC GG[ACGT]^[ACGT]CC
282 GGACCC
PflMI 180 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
297 CCAGCTCCTGG
Van91I 180 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
297 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BsaJI BsiYI BslI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EcoRII Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MnlI MspI MvaI
MvnI MwoI NciI Sau96I ScrFI
TseI