(Linear) (Six-base) MAPSORT of: KIEE6184.seq  Check: 5016  from: 1  to: 426



KIEE6184.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     266     426 
   Size:        266     160
 
AccI GT'mk_AC

Cuts at:      0      71     426 
   Size:         71     355
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     426 
   Size:        336      90
 
AflIII A'CryG_T

Cuts at:      0     263     426 
   Size:        263     163
 
AlwNI CAG_nnn'CTG

Cuts at:      0     303     426 
   Size:        303     123
 
BanII G_rGCy'C

Cuts at:      0     151     174     426 
   Size:        151      23     252

  Fragments arranged by size: 

                 252     151      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     127     426 
   Size:        127     299
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      45      79     426 
   Size:         45      34     347

  Fragments arranged by size: 

                 347      45      34
 
BlpI GC'TnA_GC

Cuts at:      0     199     250     426 
   Size:        199      51     176

  Fragments arranged by size: 

                 199     176      51
 
Bpu10I CC'TnA_GC

Cuts at:      0     145     388     426 
   Size:        145     243      38

  Fragments arranged by size: 

                 243     145      38
 
BsaHI Gr'CG_yC

Cuts at:      0      68     370     426 
   Size:         68     302      56

  Fragments arranged by size: 

                 302      68      56
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     147     426 
   Size:        147     279
 
BseYI C'CCAG_C

Cuts at:      0      51     426 
   Size:         51     375
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     426 
   Size:        244     182
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     174     426 
   Size:        151      23     252

  Fragments arranged by size: 

                 252     151      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266     426 
   Size:        266     160
 
BssHII G'CGCG_C

Cuts at:      0      33     426 
   Size:         33     393
 
BstEII G'GTnAC_C

Cuts at:      0     321     426 
   Size:        321     105
 
BtgI C'CryG_G

Cuts at:      0      11     174     209     426 
   Size:         11     163      35     217

  Fragments arranged by size: 

                 217     163      35      11
 
BtsI GCAGTG_nn'

Cuts at:      0     309     426 
   Size:        309     117
 
EaeI y'GGCC_r

Cuts at:      0     177     287     426 
   Size:        177     110     139

  Fragments arranged by size: 

                 177     139     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     205     426 
   Size:        205     221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     426 
   Size:        336      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84      95     426 
   Size:         84      11     331

  Fragments arranged by size: 

                 331      84      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     144     426 
   Size:        144     282
 
HaeII r_GCGC'y

Cuts at:      0       7      28     219     409     426 
   Size:          7      21     191     190      17

  Fragments arranged by size: 

                 191     190      21      17       7
 
MluI A'CGCG_T

Cuts at:      0     263     426 
   Size:        263     163
 
MspA1I CmG'CkG

Cuts at:      0      13     273     426 
   Size:         13     260     153

  Fragments arranged by size: 

                 260     153      13
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     186     303     426 
   Size:        186     117     123

  Fragments arranged by size: 

                 186     123     117
 
PpuMI rG'GwC_Cy

Cuts at:      0      95     426 
   Size:         95     331
 
PstI C_TGCA'G

Cuts at:      0     368     426 
   Size:        368      58
 
PvuII CAG'CTG

Cuts at:      0     273     426 
   Size:        273     153
 
SacII CC_GC'GG

Cuts at:      0      14     426 
   Size:         14     412
 
SfcI C'TryA_G

Cuts at:      0     364     426 
   Size:        364      62
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     226     258     426 
   Size:        226      32     168

  Fragments arranged by size: 

                 226     168      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI    BanII     BbsI     BcgI
     BlpI   Bpu10I    BsaHI    BseRI    BseYI     BsgI Bsp1286I    BspMI
   BssHII   BstEII     BtgI     BtsI     EaeI     EciI  Eco57MI EcoO109I
     FalI    HaeII     MluI   MspA1I    PflMI    PpuMI     PstI    PvuII
    SacII     SfcI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI